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The map label for this gene is rfbB [H]

Identifier: 120598285

GI number: 120598285

Start: 1653957

End: 1655036

Strand: Direct

Name: rfbB [H]

Synonym: Sputw3181_1463

Alternate gene names: 120598285

Gene position: 1653957-1655036 (Clockwise)

Preceding gene: 120598284

Following gene: 120598286

Centisome position: 35.13

GC content: 43.33

Gene sequence:

>1080_bases
GTGAAAATCCTAGTTACCGGTGGAGCCGGTTTTATTGGTTCTGCAGTAGTACGCCATATCATTGGCAATACCCAAGACAG
TGTGGTTAACGTAGACAAACTTACCTATGCAGGCAACTTAGAATCACTCACTAGCGTTGCGAATAATGCTCGTTATACAT
TCGAAAAAGTCGATATTTGTGATCGTGCTGAACTCGATAGAGTCTTTTTACAACACCAACCAGATGCAGTTATGCACCTA
GCTGCAGAGTCACATGTTGACCGCTCAATCACTGGCCCAAGCGACTTTATCCAAACCAATATTGTCGGTACTTATATGTT
ACTCGAAGCGGCTCGCAACTACTGGATGCAACTCGATACTGAGCGTAAATCAGCATTCCGCTTTCACCATATTTCAACCG
ATGAAGTGTATGGTGACTTGCCACATCCAGATGAACAAGAAGGACAAGTAGTAAATCAAGAACTGCCTTTATTTACCGAA
AACACGCCTTATGCACCTAGCAGCCCATATTCTGCTTCTAAAGCTTCAAGCGACCACCTAGTACGTGCATGGCTACGTAC
CTATGGTTTACCGACGATTGTCACAAATTGTTCCAACAACTATGGTCCTTATCACTTTCCAGAAAAGTTGATCCCATTGG
TAATTCTGAATGCACTCGAAGGTAAGCTACTGCCGATTTACGGTAAAGGCGACCAGATCCGCGATTGGCTATATGTCGAA
GACCATGCTCGTGCGTTATACAAAGTGGTCACTGAGGGCAAAATTGGCGAAACCTACAATATTGGTGGCCATAATGAGAA
ACAGAATTTAGAAGTAGTGCAAACCATCTGTAGCATCTTAGATTCACTAGTGCCTAAAGCCACGCCTTATGCTGAACAAA
TTACCTATGTCACCGATAGACCAGGTCATGATCGCCGCTATGCCATTGATGCTCGTAAAATGAGTAATGATCTAAATTGG
CGACCGCAAGAAACTTTTGAAACAGGTCTTCGTAAAACCATTGAATGGTATTTAGCGAATCAAGAGTGGTGTCAACATGT
ACAAGATGGCACTTACCAACGCGAGCGTTTAGGCATTTAA

Upstream 100 bases:

>100_bases
AGCTTTTTCGCTCTTACTTATAGCGGTTATTTATAGAGCTTTCCGCATTTATGCTGCTCAATTTTTTACTTAACATTTTA
TTTTATAGAGGTTTTTTGTT

Downstream 100 bases:

>100_bases
TCTATTTTATTTTAATGTCCTAGTGTTTCATAAACCTAGGGCCTATTTATCTAGGAACTGATTTTACTATGAAAGGAATT
ATTTTAGCTGGGGGCTCTGG

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKILVTGGAGFIGSAVVRHIIGNTQDSVVNVDKLTYAGNLESLTSVANNARYTFEKVDICDRAELDRVFLQHQPDAVMHL
AAESHVDRSITGPSDFIQTNIVGTYMLLEAARNYWMQLDTERKSAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTE
NTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKLLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGHDRRYAIDARKMSNDLNW
RPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI

Sequences:

>Translated_359_residues
MKILVTGGAGFIGSAVVRHIIGNTQDSVVNVDKLTYAGNLESLTSVANNARYTFEKVDICDRAELDRVFLQHQPDAVMHL
AAESHVDRSITGPSDFIQTNIVGTYMLLEAARNYWMQLDTERKSAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTE
NTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKLLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGHDRRYAIDARKMSNDLNW
RPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI
>Mature_359_residues
MKILVTGGAGFIGSAVVRHIIGNTQDSVVNVDKLTYAGNLESLTSVANNARYTFEKVDICDRAELDRVFLQHQPDAVMHL
AAESHVDRSITGPSDFIQTNIVGTYMLLEAARNYWMQLDTERKSAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTE
NTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKLLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGHDRRYAIDARKMSNDLNW
RPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=34.9854227405248, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI42516563, Length=347, Percent_Identity=24.4956772334294, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI56237023, Length=318, Percent_Identity=27.9874213836478, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI56118217, Length=318, Percent_Identity=27.9874213836478, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI189083684, Length=318, Percent_Identity=27.9874213836478, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=78.7535410764873, Blast_Score=592, Evalue=1e-171,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=71.5469613259668, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1786974, Length=361, Percent_Identity=24.6537396121884, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788366, Length=250, Percent_Identity=27.6, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=35.5029585798817, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=33.1360946745562, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=378, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=380, Percent_Identity=27.6315789473684, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=345, Percent_Identity=25.5072463768116, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=374, Percent_Identity=26.7379679144385, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=25.2199413489736, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=25.8152173913043, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40733; Mature: 40733

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIIGNTQDSVVNVDKLTYAGNLESLTSVANNARYTFEKVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHHHCCCCEEEECCCCC
DRAELDRVFLQHQPDAVMHLAAESHVDRSITGPSDFIQTNIVGTYMLLEAARNYWMQLDT
CHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
ERKSAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTENTPYAPSSPYSASKASSDHL
CHHHHEEEECCCHHHHCCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKLLPIYGKGDQIRDWLYVE
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEEH
DHARALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECC
PGHDRRYAIDARKMSNDLNWRPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI
CCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIIGNTQDSVVNVDKLTYAGNLESLTSVANNARYTFEKVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHHHCCCCEEEECCCCC
DRAELDRVFLQHQPDAVMHLAAESHVDRSITGPSDFIQTNIVGTYMLLEAARNYWMQLDT
CHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
ERKSAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTENTPYAPSSPYSASKASSDHL
CHHHHEEEECCCHHHHCCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKLLPIYGKGDQIRDWLYVE
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEEH
DHARALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECC
PGHDRRYAIDARKMSNDLNWRPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI
CCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]