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The map label for this gene is rfaH [H]

Identifier: 120598282

GI number: 120598282

Start: 1647608

End: 1648114

Strand: Direct

Name: rfaH [H]

Synonym: Sputw3181_1460

Alternate gene names: 120598282

Gene position: 1647608-1648114 (Clockwise)

Preceding gene: 120598280

Following gene: 120598283

Centisome position: 34.99

GC content: 40.83

Gene sequence:

>507_bases
ATGAACGCATGGTACCTTTTGTACTGTAAACCTCGGAGTGAAGCGAGAGCACAGCAGAACTTAGCACTTCAAAATTTAGA
GACCTACTTACCAATGGTCTCTGAAGAAAAATCACAGCGTGGACAGAAACGGATCTGCCGCGTACCGTTGTTCCCAAATT
ATTTGTTTATCAAATTTGACCCAAGCCAGACAAGCGTCAGGCAGGTCCACTCTACCCGTGGAGTAAATCGAATCGTCAAT
TGTCAAGAAAAAATGACACCCATTGATGACCGTATTATTCACACCATTCGCATGAAAGAATTCACATCTTCCCAAACCGT
ATTAGCCGAAGAATCTGAGCTAAAAATGGGGGAAAGAATTCGCTTTAAAGATGGCCCATTCGTTGATTTAGAAGGTATTT
TTCAAGAAAAATGCCCAAATAAACGCTGCCATGTTTTATTTAGTCTTATGGGGCAAGTCAATACTATTACTGTTCCAGAA
GAATCACTCGAACGAGTTTGTATATAG

Upstream 100 bases:

>100_bases
TCCTCTATGGTGAAAAACTGCTTCCATCGACATTAACATCTTTGTTATGATTGCCGCCCGTGAATGGACGTCACTTGACA
TAGGGTAATGAGATAAACAA

Downstream 100 bases:

>100_bases
ACATAGCACCAGAGTTTTAATGTGAGAGTTTTGCCCTGTATTTTGTAAGACATTGGCTATAGTTGTTATGTTTTTAACTT
TTTTAGGACAAAGTGTAATT

Product: NusG antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MNAWYLLYCKPRSEARAQQNLALQNLETYLPMVSEEKSQRGQKRICRVPLFPNYLFIKFDPSQTSVRQVHSTRGVNRIVN
CQEKMTPIDDRIIHTIRMKEFTSSQTVLAEESELKMGERIRFKDGPFVDLEGIFQEKCPNKRCHVLFSLMGQVNTITVPE
ESLERVCI

Sequences:

>Translated_168_residues
MNAWYLLYCKPRSEARAQQNLALQNLETYLPMVSEEKSQRGQKRICRVPLFPNYLFIKFDPSQTSVRQVHSTRGVNRIVN
CQEKMTPIDDRIIHTIRMKEFTSSQTVLAEESELKMGERIRFKDGPFVDLEGIFQEKCPNKRCHVLFSLMGQVNTITVPE
ESLERVCI
>Mature_168_residues
MNAWYLLYCKPRSEARAQQNLALQNLETYLPMVSEEKSQRGQKRICRVPLFPNYLFIKFDPSQTSVRQVHSTRGVNRIVN
CQEKMTPIDDRIIHTIRMKEFTSSQTVLAEESELKMGERIRFKDGPFVDLEGIFQEKCPNKRCHVLFSLMGQVNTITVPE
ESLERVCI

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=152, Percent_Identity=30.2631578947368, Blast_Score=87, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 19613; Mature: 19613

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAWYLLYCKPRSEARAQQNLALQNLETYLPMVSEEKSQRGQKRICRVPLFPNYLFIKFD
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCEEEEEEC
PSQTSVRQVHSTRGVNRIVNCQEKMTPIDDRIIHTIRMKEFTSSQTVLAEESELKMGERI
CCHHHHHHHHHHCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
RFKDGPFVDLEGIFQEKCPNKRCHVLFSLMGQVNTITVPEESLERVCI
EECCCCEECHHHHHHHHCCCHHHHHHHHHHCCCCEEECCHHHHHHCCC
>Mature Secondary Structure
MNAWYLLYCKPRSEARAQQNLALQNLETYLPMVSEEKSQRGQKRICRVPLFPNYLFIKFD
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCEEEEEEC
PSQTSVRQVHSTRGVNRIVNCQEKMTPIDDRIIHTIRMKEFTSSQTVLAEESELKMGERI
CCHHHHHHHHHHCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
RFKDGPFVDLEGIFQEKCPNKRCHVLFSLMGQVNTITVPEESLERVCI
EECCCCEECHHHHHHHHCCCHHHHHHHHHHCCCCEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]