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The map label for this gene is fliF

Identifier: 120598250

GI number: 120598250

Start: 1614079

End: 1615761

Strand: Direct

Name: fliF

Synonym: Sputw3181_1427

Alternate gene names: 120598250

Gene position: 1614079-1615761 (Clockwise)

Preceding gene: 120598249

Following gene: 120598251

Centisome position: 34.28

GC content: 45.45

Gene sequence:

>1683_bases
ATGATTGTCGGGTCTAATTCCGATTTAGCAGACCAAGGTGTCCAAGAAGAACATAAGTCAGGTGTGATGGGAAGCCTTGG
CGGTGTCGATATGATGCGCCAAATTACCATGATCCTTGCGTTGGCAATCTGCTTAGCCCTAGCCGTTTTTGTGATGTTAT
GGGCTCAGGAACCTGAGTATCGTCCATTAGGAAAAATGGAAACTCAGGAAATGGTACAAGTACTCGATGTCTTGGATAAG
AATAAGATCAAGTATCAGATCGAGGTTGATGTTATTAAAGTACCTGAGGATAAATATCAAGAAGTCAAACTGATGTTAAG
CCGAGCAGGGATTGACGGTGGCACTACAAGTAAAGATTTTTTAACCCAAGACAGTGGTTTTGGTGTCAGCCAAAGGATGG
AACAAGCGCGTCTTAAACACAGCCAAGAAGAAAACCTTGCCCGCGCTATTGAGCAGCTACAAAGTGTCAGTCGCGCAAAA
GTGATTCTCGCACTTCCCAAAGAAAACGTCTTTGCCCGTAATACTTCCCAACCTAGTGCAACCGTGGTTATCAATACCCG
TCGTGGCGGTTTAGGCCAAGGAGAGGTGGATGCCATTGTTGATATTGTTGCCTCAGCCGTGCAAGGGTTGGAACCATCGC
GTGTTACCGTCACTGATTCAAATGGTCGTTTGCTTAACTCTGGGAGCCAAGATGGCGTTTCCGCTAGGGCGCGTAGAGAA
CTCGAATTAGTACAACAAAAAGAAGCTGAATATCGCACAAAAATTGAATCAATTTTAGTTCCCATTTTAGGGCCTGATAA
TTTTACTTCCCAAGTCGATGTTAGTATGGACTTTACCGCAGTCGAACAAACAGCCAAGCGCTTTAATCCTGATTTACCAG
CATTACGCAGTGAAATGACGGTTGAGAATAACTCTACCGGTGGCACTACGGGGGGGATCCCTGGCGCTCTGTCAAATCAA
CCGCCAATGGAGTCAGATATTCCTGAAGATGCAACCAATGCATCTGAAAAAGTGGTTGCGGGTAACTCTCACCGTGAAGC
AACACGAAATTTTGAGCTCGATACCACCATTAGCCATACCCGTCAGCAAATTGGGGTAGTCAGACGAATTAGTGTTTCAG
TTGCTGTGGATTTTAAACCCGGTGCTGCAGATGAAAATGGGCAAGTGGCACGCGTTGCCCGTACTGAGCAAGAGTTAACC
AACATTCGTCGTTTACTAGAAGGTGCGGTAGGATTCAGCACACAACGTGGTGATGTGTTGGAAGTGGTTACCGTTCCTTT
TATGGATCAACTGATGGAAGACATACCATCTCCTGAATTATGGGAACAGCCGTGGTTCTGGCGTGCGGTAAAATTGGGTG
TTGGCGCCTTAGTGATCCTAGTATTGATATTGGCCGTGGTTCGTCCAATGCTAAAACGCCTTATTTACCCAGATAGCGTC
AATATGCCAGATGATTCAAGACTGGGTAATGAACTGGCGGAAATTGAAGATCAGTACGCTGCCGACACCTTAGGTATGCT
TAATACCAAAGAAGCAGAATACAGTTATGCCGATGATGGCTCAATTCTTATCCCTAATCTTCATAAAGATGATGATATGA
TTAAAGCTATCCGTGCGCTTGTGGCAAATGAGCCCGAGCTTTCCACTCAAGTCGTGAAAAACTGGTTACAAGACAATGGC
TGA

Upstream 100 bases:

>100_bases
AGTGCGTAATAAACTTGTCGAAGCTTATAAAGAAATAATGAGTATGCCTGTTTAGGCCCGAGAATAACCCAATCAGCAGG
TACGAATCGTGAGCACAGAA

Downstream 100 bases:

>100_bases
GAATAAAACAAAAGAAGCCGCTGAAGTCTCAAGCTTTAATGTGAAGGACATGAGCGGTATCGAGAAGACGGCAATTTTAC
TCTTAAGTTTAAGCGAAGCC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MIVGSNSDLADQGVQEEHKSGVMGSLGGVDMMRQITMILALAICLALAVFVMLWAQEPEYRPLGKMETQEMVQVLDVLDK
NKIKYQIEVDVIKVPEDKYQEVKLMLSRAGIDGGTTSKDFLTQDSGFGVSQRMEQARLKHSQEENLARAIEQLQSVSRAK
VILALPKENVFARNTSQPSATVVINTRRGGLGQGEVDAIVDIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGVSARARRE
LELVQQKEAEYRTKIESILVPILGPDNFTSQVDVSMDFTAVEQTAKRFNPDLPALRSEMTVENNSTGGTTGGIPGALSNQ
PPMESDIPEDATNASEKVVAGNSHREATRNFELDTTISHTRQQIGVVRRISVSVAVDFKPGAADENGQVARVARTEQELT
NIRRLLEGAVGFSTQRGDVLEVVTVPFMDQLMEDIPSPELWEQPWFWRAVKLGVGALVILVLILAVVRPMLKRLIYPDSV
NMPDDSRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSILIPNLHKDDDMIKAIRALVANEPELSTQVVKNWLQDNG

Sequences:

>Translated_560_residues
MIVGSNSDLADQGVQEEHKSGVMGSLGGVDMMRQITMILALAICLALAVFVMLWAQEPEYRPLGKMETQEMVQVLDVLDK
NKIKYQIEVDVIKVPEDKYQEVKLMLSRAGIDGGTTSKDFLTQDSGFGVSQRMEQARLKHSQEENLARAIEQLQSVSRAK
VILALPKENVFARNTSQPSATVVINTRRGGLGQGEVDAIVDIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGVSARARRE
LELVQQKEAEYRTKIESILVPILGPDNFTSQVDVSMDFTAVEQTAKRFNPDLPALRSEMTVENNSTGGTTGGIPGALSNQ
PPMESDIPEDATNASEKVVAGNSHREATRNFELDTTISHTRQQIGVVRRISVSVAVDFKPGAADENGQVARVARTEQELT
NIRRLLEGAVGFSTQRGDVLEVVTVPFMDQLMEDIPSPELWEQPWFWRAVKLGVGALVILVLILAVVRPMLKRLIYPDSV
NMPDDSRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSILIPNLHKDDDMIKAIRALVANEPELSTQVVKNWLQDNG
>Mature_560_residues
MIVGSNSDLADQGVQEEHKSGVMGSLGGVDMMRQITMILALAICLALAVFVMLWAQEPEYRPLGKMETQEMVQVLDVLDK
NKIKYQIEVDVIKVPEDKYQEVKLMLSRAGIDGGTTSKDFLTQDSGFGVSQRMEQARLKHSQEENLARAIEQLQSVSRAK
VILALPKENVFARNTSQPSATVVINTRRGGLGQGEVDAIVDIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGVSARARRE
LELVQQKEAEYRTKIESILVPILGPDNFTSQVDVSMDFTAVEQTAKRFNPDLPALRSEMTVENNSTGGTTGGIPGALSNQ
PPMESDIPEDATNASEKVVAGNSHREATRNFELDTTISHTRQQIGVVRRISVSVAVDFKPGAADENGQVARVARTEQELT
NIRRLLEGAVGFSTQRGDVLEVVTVPFMDQLMEDIPSPELWEQPWFWRAVKLGVGALVILVLILAVVRPMLKRLIYPDSV
NMPDDSRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSILIPNLHKDDDMIKAIRALVANEPELSTQVVKNWLQDNG

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=557, Percent_Identity=31.5978456014363, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 61466; Mature: 61466

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGSNSDLADQGVQEEHKSGVMGSLGGVDMMRQITMILALAICLALAVFVMLWAQEPEY
CEECCCCCHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPLGKMETQEMVQVLDVLDKNKIKYQIEVDVIKVPEDKYQEVKLMLSRAGIDGGTTSKDF
CCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHH
LTQDSGFGVSQRMEQARLKHSQEENLARAIEQLQSVSRAKVILALPKENVFARNTSQPSA
HCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCEECCCCCCCE
TVVINTRRGGLGQGEVDAIVDIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGVSARARRE
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCHHHHHHHH
LELVQQKEAEYRTKIESILVPILGPDNFTSQVDVSMDFTAVEQTAKRFNPDLPALRSEMT
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHE
VENNSTGGTTGGIPGALSNQPPMESDIPEDATNASEKVVAGNSHREATRNFELDTTISHT
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCHHHHHH
RQQIGVVRRISVSVAVDFKPGAADENGQVARVARTEQELTNIRRLLEGAVGFSTQRGDVL
HHHHHHHHEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
EVVTVPFMDQLMEDIPSPELWEQPWFWRAVKLGVGALVILVLILAVVRPMLKRLIYPDSV
EHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NMPDDSRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSILIPNLHKDDDMIKAIRAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
VANEPELSTQVVKNWLQDNG
HCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVGSNSDLADQGVQEEHKSGVMGSLGGVDMMRQITMILALAICLALAVFVMLWAQEPEY
CEECCCCCHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPLGKMETQEMVQVLDVLDKNKIKYQIEVDVIKVPEDKYQEVKLMLSRAGIDGGTTSKDF
CCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHH
LTQDSGFGVSQRMEQARLKHSQEENLARAIEQLQSVSRAKVILALPKENVFARNTSQPSA
HCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCEECCCCCCCE
TVVINTRRGGLGQGEVDAIVDIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGVSARARRE
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCHHHHHHHH
LELVQQKEAEYRTKIESILVPILGPDNFTSQVDVSMDFTAVEQTAKRFNPDLPALRSEMT
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHE
VENNSTGGTTGGIPGALSNQPPMESDIPEDATNASEKVVAGNSHREATRNFELDTTISHT
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCHHHHHH
RQQIGVVRRISVSVAVDFKPGAADENGQVARVARTEQELTNIRRLLEGAVGFSTQRGDVL
HHHHHHHHEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
EVVTVPFMDQLMEDIPSPELWEQPWFWRAVKLGVGALVILVLILAVVRPMLKRLIYPDSV
EHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NMPDDSRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSILIPNLHKDDDMIKAIRAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
VANEPELSTQVVKNWLQDNG
HCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]