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The map label for this gene is lcfB [H]

Identifier: 120598209

GI number: 120598209

Start: 1575311

End: 1576672

Strand: Direct

Name: lcfB [H]

Synonym: Sputw3181_1386

Alternate gene names: 120598209

Gene position: 1575311-1576672 (Clockwise)

Preceding gene: 120598208

Following gene: 120598210

Centisome position: 33.46

GC content: 41.78

Gene sequence:

>1362_bases
ATGTTGTCACATATAAAAGCTGATAATCCAAACTTAGCGTGGTGGGAACCTCAGCTCGGTTGGCTGACATATGGCGATAT
TCATCAGAAAGCGCTCCAGCTTCAGCAGACTTATCCAAGCTCAAAATGCTTAATTTACTTACCTTTTTTTAATCGTCTTT
CACACGTTCTTCATTACATTGTCGCTTTAAGACTGGGACATCCCCTCATGCTTGCTGACCCATTGATGAGTCACGAGCAG
CGGCAATTGACCATTGATAATTATGGTGTTAATGCCTGGATTGATGAGTCCGGTATGTTGCATGAGTTGACAAAAAGTAA
AACAACTCAGGCTAGGTTGCATCCTTCTCTTGCTTTGCTATTGCCAACCTCCGGAAGTACCGGTGCATTTAAATGGGTAC
GCTTATCTCACAAAAACCTCCAAAGTAACGCGCTAGCAATTGCTGAGTATTTACAGATAAATCAAGATGAGCGGGCAATT
ACAAGTTTGCCATTCTTTTACTCTTATGGTCTTTCTGTTCTCAATAGTCATCTTGCCGTTGGTGCCGCGTTAGTTCAAAG
CGAATATAGCACAATGGATCGCCAGTTTTGGTCCTTGCTTAGTAACTTGGAGGTAACTAGTTTTGCGGGGGTCCCTTTTA
CCTATCAAACATTAAATAAATTGAAGTTTGACTGGTCGAGCTACCCATGTCTTCGCACTATGACGCAAGCAGGCGGTCGG
CTTGATTCCGAATTAGCGCGGCACTTTGCTACCGAAGCTTTGAAACGACATAAACGCTTTTTTATTATGTACGGTCAAAC
CGAGGCAGCTCCAAGAATGGCTTGGATAGCTGAAAATGAAATCGTTGAGCATCCCAATTCTATCGGTCGTCCCATTCCTG
GCGGTGAATTTAATTTAAAACCAATTGACGGATTAGCGGAAGGGCAAGGTGAGCTTATCTACCGTGGACCAAACGTGATG
ATGGGTTATGCCGAAAGTGAGTTCGATCTTGCCAAGGGCGCTGAATATAAAGAGTTAGCTACTGGAGATGTTGCTTATTG
TGATACAGAGGGACGCTATTACCTTTGTGGGCGTTTAAAACGTTTTATCAAACTTTTCGGTAAACGTACTAACCTTGTAG
ACATTGAAACATTTCTCGAAAATAAATCTATTATTACTGCTTGCCTTAATAAAGATGATGAGTTGCTGATCGCATATGAG
CCAACGGATCAAGATATAGATGCTGTGAAAAAACAACTTTCACTCTGGTTACGTATTCCTATTAACGTGATTAAGTTCAT
CCTTGTTCCTGTTTTGCCTCGAACTTCAAATCTTAAGATTGACTATAATACGCTGCCGCAATTATTGGAGTCATCATCAT
GA

Upstream 100 bases:

>100_bases
TGGCCTCTAATATGGCAAGTTATGTCACGGGTCAGGTGATAGGTGTTGATGGGGGGATGCTTATTTGAATATCTGCCTTC
AAGGGTGTGGCGTCACTAAA

Downstream 100 bases:

>100_bases
ATTGGCTTTGGGATCACTCCATATTCGCTTTGCCACAGGTTGAGAAGGAAGCGCTGTTATTAGCTGAATTGAATAAGCTG
ACCTCTCATCACGAGGTCAA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MLSHIKADNPNLAWWEPQLGWLTYGDIHQKALQLQQTYPSSKCLIYLPFFNRLSHVLHYIVALRLGHPLMLADPLMSHEQ
RQLTIDNYGVNAWIDESGMLHELTKSKTTQARLHPSLALLLPTSGSTGAFKWVRLSHKNLQSNALAIAEYLQINQDERAI
TSLPFFYSYGLSVLNSHLAVGAALVQSEYSTMDRQFWSLLSNLEVTSFAGVPFTYQTLNKLKFDWSSYPCLRTMTQAGGR
LDSELARHFATEALKRHKRFFIMYGQTEAAPRMAWIAENEIVEHPNSIGRPIPGGEFNLKPIDGLAEGQGELIYRGPNVM
MGYAESEFDLAKGAEYKELATGDVAYCDTEGRYYLCGRLKRFIKLFGKRTNLVDIETFLENKSIITACLNKDDELLIAYE
PTDQDIDAVKKQLSLWLRIPINVIKFILVPVLPRTSNLKIDYNTLPQLLESSS

Sequences:

>Translated_453_residues
MLSHIKADNPNLAWWEPQLGWLTYGDIHQKALQLQQTYPSSKCLIYLPFFNRLSHVLHYIVALRLGHPLMLADPLMSHEQ
RQLTIDNYGVNAWIDESGMLHELTKSKTTQARLHPSLALLLPTSGSTGAFKWVRLSHKNLQSNALAIAEYLQINQDERAI
TSLPFFYSYGLSVLNSHLAVGAALVQSEYSTMDRQFWSLLSNLEVTSFAGVPFTYQTLNKLKFDWSSYPCLRTMTQAGGR
LDSELARHFATEALKRHKRFFIMYGQTEAAPRMAWIAENEIVEHPNSIGRPIPGGEFNLKPIDGLAEGQGELIYRGPNVM
MGYAESEFDLAKGAEYKELATGDVAYCDTEGRYYLCGRLKRFIKLFGKRTNLVDIETFLENKSIITACLNKDDELLIAYE
PTDQDIDAVKKQLSLWLRIPINVIKFILVPVLPRTSNLKIDYNTLPQLLESSS
>Mature_453_residues
MLSHIKADNPNLAWWEPQLGWLTYGDIHQKALQLQQTYPSSKCLIYLPFFNRLSHVLHYIVALRLGHPLMLADPLMSHEQ
RQLTIDNYGVNAWIDESGMLHELTKSKTTQARLHPSLALLLPTSGSTGAFKWVRLSHKNLQSNALAIAEYLQINQDERAI
TSLPFFYSYGLSVLNSHLAVGAALVQSEYSTMDRQFWSLLSNLEVTSFAGVPFTYQTLNKLKFDWSSYPCLRTMTQAGGR
LDSELARHFATEALKRHKRFFIMYGQTEAAPRMAWIAENEIVEHPNSIGRPIPGGEFNLKPIDGLAEGQGELIYRGPNVM
MGYAESEFDLAKGAEYKELATGDVAYCDTEGRYYLCGRLKRFIKLFGKRTNLVDIETFLENKSIITACLNKDDELLIAYE
PTDQDIDAVKKQLSLWLRIPINVIKFILVPVLPRTSNLKIDYNTLPQLLESSS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789201, Length=311, Percent_Identity=27.3311897106109, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693145, Length=296, Percent_Identity=27.7027027027027, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=280, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559526, Length=305, Percent_Identity=28.1967213114754, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=290, Percent_Identity=26.551724137931, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859661, Length=285, Percent_Identity=28.7719298245614, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 51413; Mature: 51413

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHIKADNPNLAWWEPQLGWLTYGDIHQKALQLQQTYPSSKCLIYLPFFNRLSHVLHYI
CCCCCCCCCCCCEEECCCCCCEEECHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
VALRLGHPLMLADPLMSHEQRQLTIDNYGVNAWIDESGMLHELTKSKTTQARLHPSLALL
HHHHCCCCCEEECHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHCCCHHHHCCCCEEEE
LPTSGSTGAFKWVRLSHKNLQSNALAIAEYLQINQDERAITSLPFFYSYGLSVLNSHLAV
EECCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
GAALVQSEYSTMDRQFWSLLSNLEVTSFAGVPFTYQTLNKLKFDWSSYPCLRTMTQAGGR
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEHHHHHHHCCCCCCCHHHHHHHHHCCC
LDSELARHFATEALKRHKRFFIMYGQTEAAPRMAWIAENEIVEHPNSIGRPIPGGEFNLK
CHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEECCHHHHCCHHCCCCCCCCCCCCC
PIDGLAEGQGELIYRGPNVMMGYAESEFDLAKGAEYKELATGDVAYCDTEGRYYLCGRLK
CCCCCCCCCCCEEEECCCEEEECCCCCCHHHCCCCHHHHCCCCEEEECCCCCEEHHHHHH
RFIKLFGKRTNLVDIETFLENKSIITACLNKDDELLIAYEPTDQDIDAVKKQLSLWLRIP
HHHHHHCCCCCEEEHHHHHCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHEEEECH
INVIKFILVPVLPRTSNLKIDYNTLPQLLESSS
HHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCC
>Mature Secondary Structure
MLSHIKADNPNLAWWEPQLGWLTYGDIHQKALQLQQTYPSSKCLIYLPFFNRLSHVLHYI
CCCCCCCCCCCCEEECCCCCCEEECHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
VALRLGHPLMLADPLMSHEQRQLTIDNYGVNAWIDESGMLHELTKSKTTQARLHPSLALL
HHHHCCCCCEEECHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHCCCHHHHCCCCEEEE
LPTSGSTGAFKWVRLSHKNLQSNALAIAEYLQINQDERAITSLPFFYSYGLSVLNSHLAV
EECCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
GAALVQSEYSTMDRQFWSLLSNLEVTSFAGVPFTYQTLNKLKFDWSSYPCLRTMTQAGGR
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEHHHHHHHCCCCCCCHHHHHHHHHCCC
LDSELARHFATEALKRHKRFFIMYGQTEAAPRMAWIAENEIVEHPNSIGRPIPGGEFNLK
CHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEECCHHHHCCHHCCCCCCCCCCCCC
PIDGLAEGQGELIYRGPNVMMGYAESEFDLAKGAEYKELATGDVAYCDTEGRYYLCGRLK
CCCCCCCCCCCEEEECCCEEEECCCCCCHHHCCCCHHHHCCCCEEEECCCCCEEHHHHHH
RFIKLFGKRTNLVDIETFLENKSIITACLNKDDELLIAYEPTDQDIDAVKKQLSLWLRIP
HHHHHHCCCCCEEEHHHHHCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHEEEECH
INVIKFILVPVLPRTSNLKIDYNTLPQLLESSS
HHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]