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The map label for this gene is thrA

Identifier: 120598100

GI number: 120598100

Start: 1455045

End: 1457513

Strand: Direct

Name: thrA

Synonym: Sputw3181_1277

Alternate gene names: 120598100

Gene position: 1455045-1457513 (Clockwise)

Preceding gene: 120598098

Following gene: 120598101

Centisome position: 30.9

GC content: 49.25

Gene sequence:

>2469_bases
ATGAAAGTAATGAAATTTGGTGGTACCTCGCTTGCAAATTGGCAGCGATTCTCGATGGCGGCGGATATCGTTGCTAAGTC
ATCTAAGCTTGAGCCAATAGCCACAGTGCTATCGGCCCCGGCGACTGTGACAAATGCGTTACTAGAAATGGTTGATGTAG
CCGTTAAAGGTGAAGATTATTCGTCTGTGCTGCAGCATGTTGAGCGGGTATTTACCTCGCTATACCAAGATGCTGTTACT
TCAATGTTGTCGAGTGAGCAGAGTGAACAGCTCTTTTCTGGACTTAATGCGCAGCTGGTGATTTGGCAGGATAGATTACG
TGGCATTAGCTTACTGCAAGAGTGTCCCGATGGCATACGTGCTGAAATCGTGGTGGCAGGTGAGCGTTTATCTGCAGCCT
TGATGGAACAAGTGATGCTATCTAAGGGGATCACGTCTGCTCAACTCGATCCGCGCGCCTTGTTTCTAGGTCGAGGTCGG
CCGTTAGAATCCGTGGTCGATATCGCTATAAGTAAGCCAAGGTTTCGCGCGTTGGCGCTGGGTGAAAAGCGCGTATGGGT
AATGCCGGGTTTCACCGCCGCCGATGAACAGGGCAAAGTGGTGACGTTAGGCCGTAATGGTTCGGACTATTCTGCTGCCG
TGCTGGCCGCCTGTTTAGATGCTTCAAGTTGTGAAATCTGGACCGATGTTGACGGTGTTTATAATACCGATCCCCGGGTG
GTTGCCGATGCCAAGTTGTTGTCACAACTCAGTTATCAAGAGGCGATGGAACTCTCCTATTTTGGCGCTAAAGTGCTGCA
TCCTAAGACGATTGCGCCCATCGCTCAGTTCCATATTCCCTGTTATATCAAAAATAGTTTTAACCCCGATGCGCCCGGCA
CTCTGGTATCAAACCAAGCCGATGAAACTGGCTTGCAGGTTAAAGCTATCTCCAATTTAGATAACCAGACCATGTTCGAT
ATATCAGGCCCAGGCATGAAAGGCATGGTTGGCATGGCGAGCCGTACTCTCGCGGCGATTTCCCGCAGCGGCGTGTCAGT
CTCTTTAATCACCCAAAGCTCCTCTGAATACAGTATTAGCTTCTGTGTGGCGACGTCTGACTCGGCCAAAGTGAAATGGG
CCTTAGAGCAAGAGTTTGAATTAGAAATAAAAAGTGACTTGCTTGAACCTATTGCTATGCGTCATGAGCTGGCAATTGTG
TCTTTGATTGGTGATGGCATGCGCACCCACAAAGGCGTGGCAGCGCGTTTCTTCCAAGCGTTAGCGCAGGCGAGCGTGAA
CATTATCGCCATAGCCCAAGGTTCGTCCGAGCGCTCGATTTCGACTGTGATCGAGCAGCGTAAGACTAAGCATGCGGTGG
CGGCTTGTCATCAAGGCTTTTTCGATGTGCAGCAGTATTTAGATGTGTTTTTAGTGGGTTGCGGCAATGTGGGGGCTGGC
CTGCTTGAGCAGATAAAACAACAGTCCGCCATGCTTAAAGAGCAGCACATCAGTATCCGTGTTTGCGGTATAGTGAATTC
GAACAAGATGTTACTCGACAGTGCGGGCATCGATTTAAGCAATTGGTCTAGCTTGCTTGCCGATAGCCAGCAAGCTTGCG
ATATCCCAGCATTACTCGCATGGGCGAAAGAGCAACAATTGCTTAATCCAGTGCTGGTGGATTGCACTTCGAGCGAGCAA
GTATCGGATCTGTATTTGGATGTGATGAACTCAGGTTTGCATGTGGTCACGCCGAACAAAAAGTCGAACACTCGCGATTA
TGCTTATTATCAAGCACTGCGCCAAACGGCATTAAAACAGCGTCGCCAATTCTTATACGAAACCACAGTCGGTGCGGGTT
TACCTGTGATCGATAACCTGAAAAAACTGTTATTTGCGGGTGACAAACTGCATAAATTTAACGGTATTTTATCGGGCTCT
TTATCCTTTATCTTCGGCATGCTCGATGAAGGTATGACGCTTTCTGAAGCGACTGGTCTGGCGCGAGAAAAATGTTTTAC
TGAGCCCGATCCCCGTGATGATTTGAGTGGTATGGATGTGGCGCGTAAGGTGCTCATTCTTGCCCGTGAAGTGGGACTAA
AGTTAGAGCTCAGTGACGTGCAGGTTGAATCTGTATTGCCAGCCGAGTTTGATGATTCAGGTGATGTTGAAAGCTTTATG
GCACGATTACCGCAAGTAGATGCTGCAATTGCCGCAAAAGTGGCGCAGGCCAAAGCCCAAGGTAAAGTGCTGCGTTATGT
TGGGCAAATTGAGGATGGTGTGTGCCGTGTGCGTATCGCCGAAGTGGATGCACAGGATCCGCTTTATAGCGTTAAAGGCG
GTGAAAATGCCTTGGCGTTCTATAGCCGCTATTATCAGCCCATTCCCTTTGTGCTACGCGGCTATGGCGCAGGGACTGAA
GTGACCGCTGCTGGCGCCTTTGCCGATGTATTAAGAACCTTGAATTGGACTCGTGAGGTGAGTGTATGA

Upstream 100 bases:

>100_bases
ACCCTAAAGAATTTTAACGATGAGCCCGCTTCCCACAAAGAAGCGGGCTTTTTTGTTTCTAGCTCTTATTTCTAAGTCGG
GCGCGGTACAGGAGAACAAG

Downstream 100 bases:

>100_bases
GTTTGACTGTTTACGCTCCAGCCTCTATGGGTAACGTGGGCGTCGGATTTGATTTACTCGGTGCTGCATTAGCGCCGATT
GATGGTAGTTTACTGGGCGA

Product: bifunctional aspartokinase I/homoserine dehydrogenase I

Products: NA

Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 822; Mature: 822

Protein sequence:

>822_residues
MKVMKFGGTSLANWQRFSMAADIVAKSSKLEPIATVLSAPATVTNALLEMVDVAVKGEDYSSVLQHVERVFTSLYQDAVT
SMLSSEQSEQLFSGLNAQLVIWQDRLRGISLLQECPDGIRAEIVVAGERLSAALMEQVMLSKGITSAQLDPRALFLGRGR
PLESVVDIAISKPRFRALALGEKRVWVMPGFTAADEQGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRV
VADAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQFHIPCYIKNSFNPDAPGTLVSNQADETGLQVKAISNLDNQTMFD
ISGPGMKGMVGMASRTLAAISRSGVSVSLITQSSSEYSISFCVATSDSAKVKWALEQEFELEIKSDLLEPIAMRHELAIV
SLIGDGMRTHKGVAARFFQALAQASVNIIAIAQGSSERSISTVIEQRKTKHAVAACHQGFFDVQQYLDVFLVGCGNVGAG
LLEQIKQQSAMLKEQHISIRVCGIVNSNKMLLDSAGIDLSNWSSLLADSQQACDIPALLAWAKEQQLLNPVLVDCTSSEQ
VSDLYLDVMNSGLHVVTPNKKSNTRDYAYYQALRQTALKQRRQFLYETTVGAGLPVIDNLKKLLFAGDKLHKFNGILSGS
LSFIFGMLDEGMTLSEATGLAREKCFTEPDPRDDLSGMDVARKVLILAREVGLKLELSDVQVESVLPAEFDDSGDVESFM
ARLPQVDAAIAAKVAQAKAQGKVLRYVGQIEDGVCRVRIAEVDAQDPLYSVKGGENALAFYSRYYQPIPFVLRGYGAGTE
VTAAGAFADVLRTLNWTREVSV

Sequences:

>Translated_822_residues
MKVMKFGGTSLANWQRFSMAADIVAKSSKLEPIATVLSAPATVTNALLEMVDVAVKGEDYSSVLQHVERVFTSLYQDAVT
SMLSSEQSEQLFSGLNAQLVIWQDRLRGISLLQECPDGIRAEIVVAGERLSAALMEQVMLSKGITSAQLDPRALFLGRGR
PLESVVDIAISKPRFRALALGEKRVWVMPGFTAADEQGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRV
VADAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQFHIPCYIKNSFNPDAPGTLVSNQADETGLQVKAISNLDNQTMFD
ISGPGMKGMVGMASRTLAAISRSGVSVSLITQSSSEYSISFCVATSDSAKVKWALEQEFELEIKSDLLEPIAMRHELAIV
SLIGDGMRTHKGVAARFFQALAQASVNIIAIAQGSSERSISTVIEQRKTKHAVAACHQGFFDVQQYLDVFLVGCGNVGAG
LLEQIKQQSAMLKEQHISIRVCGIVNSNKMLLDSAGIDLSNWSSLLADSQQACDIPALLAWAKEQQLLNPVLVDCTSSEQ
VSDLYLDVMNSGLHVVTPNKKSNTRDYAYYQALRQTALKQRRQFLYETTVGAGLPVIDNLKKLLFAGDKLHKFNGILSGS
LSFIFGMLDEGMTLSEATGLAREKCFTEPDPRDDLSGMDVARKVLILAREVGLKLELSDVQVESVLPAEFDDSGDVESFM
ARLPQVDAAIAAKVAQAKAQGKVLRYVGQIEDGVCRVRIAEVDAQDPLYSVKGGENALAFYSRYYQPIPFVLRGYGAGTE
VTAAGAFADVLRTLNWTREVSV
>Mature_822_residues
MKVMKFGGTSLANWQRFSMAADIVAKSSKLEPIATVLSAPATVTNALLEMVDVAVKGEDYSSVLQHVERVFTSLYQDAVT
SMLSSEQSEQLFSGLNAQLVIWQDRLRGISLLQECPDGIRAEIVVAGERLSAALMEQVMLSKGITSAQLDPRALFLGRGR
PLESVVDIAISKPRFRALALGEKRVWVMPGFTAADEQGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRV
VADAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQFHIPCYIKNSFNPDAPGTLVSNQADETGLQVKAISNLDNQTMFD
ISGPGMKGMVGMASRTLAAISRSGVSVSLITQSSSEYSISFCVATSDSAKVKWALEQEFELEIKSDLLEPIAMRHELAIV
SLIGDGMRTHKGVAARFFQALAQASVNIIAIAQGSSERSISTVIEQRKTKHAVAACHQGFFDVQQYLDVFLVGCGNVGAG
LLEQIKQQSAMLKEQHISIRVCGIVNSNKMLLDSAGIDLSNWSSLLADSQQACDIPALLAWAKEQQLLNPVLVDCTSSEQ
VSDLYLDVMNSGLHVVTPNKKSNTRDYAYYQALRQTALKQRRQFLYETTVGAGLPVIDNLKKLLFAGDKLHKFNGILSGS
LSFIFGMLDEGMTLSEATGLAREKCFTEPDPRDDLSGMDVARKVLILAREVGLKLELSDVQVESVLPAEFDDSGDVESFM
ARLPQVDAAIAAKVAQAKAQGKVLRYVGQIEDGVCRVRIAEVDAQDPLYSVKGGENALAFYSRYYQPIPFVLRGYGAGTE
VTAAGAFADVLRTLNWTREVSV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=820, Percent_Identity=57.3170731707317, Blast_Score=940, Evalue=0.0,
Organism=Escherichia coli, GI1790376, Length=817, Percent_Identity=30.9669522643819, Blast_Score=364, Evalue=1e-101,
Organism=Escherichia coli, GI1790455, Length=469, Percent_Identity=30.0639658848614, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322599, Length=338, Percent_Identity=31.0650887573964, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320893, Length=505, Percent_Identity=26.1386138613861, Blast_Score=144, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 89197; Mature: 89197

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMKFGGTSLANWQRFSMAADIVAKSSKLEPIATVLSAPATVTNALLEMVDVAVKGEDY
CCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
SSVLQHVERVFTSLYQDAVTSMLSSEQSEQLFSGLNAQLVIWQDRLRGISLLQECPDGIR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCE
AEIVVAGERLSAALMEQVMLSKGITSAQLDPRALFLGRGRPLESVVDIAISKPRFRALAL
EEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEE
GEKRVWVMPGFTAADEQGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRV
CCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCE
VADAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQFHIPCYIKNSFNPDAPGTLVSNQA
EHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCEECCCC
DETGLQVKAISNLDNQTMFDISGPGMKGMVGMASRTLAAISRSGVSVSLITQSSSEYSIS
CCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
FCVATSDSAKVKWALEQEFELEIKSDLLEPIAMRHELAIVSLIGDGMRTHKGVAARFFQA
EEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LAQASVNIIAIAQGSSERSISTVIEQRKTKHAVAACHQGFFDVQQYLDVFLVGCGNVGAG
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHH
LLEQIKQQSAMLKEQHISIRVCGIVNSNKMLLDSAGIDLSNWSSLLADSQQACDIPALLA
HHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCCHHHHHHHHHCCHHHCCCHHHHH
WAKEQQLLNPVLVDCTSSEQVSDLYLDVMNSGLHVVTPNKKSNTRDYAYYQALRQTALKQ
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
RRQFLYETTVGAGLPVIDNLKKLLFAGDKLHKFNGILSGSLSFIFGMLDEGMTLSEATGL
HHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AREKCFTEPDPRDDLSGMDVARKVLILAREVGLKLELSDVQVESVLPAEFDDSGDVESFM
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCCCCCCCCHHHHH
ARLPQVDAAIAAKVAQAKAQGKVLRYVGQIEDGVCRVRIAEVDAQDPLYSVKGGENALAF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCHHHHH
YSRYYQPIPFVLRGYGAGTEVTAAGAFADVLRTLNWTREVSV
HHHHCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MKVMKFGGTSLANWQRFSMAADIVAKSSKLEPIATVLSAPATVTNALLEMVDVAVKGEDY
CCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
SSVLQHVERVFTSLYQDAVTSMLSSEQSEQLFSGLNAQLVIWQDRLRGISLLQECPDGIR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCE
AEIVVAGERLSAALMEQVMLSKGITSAQLDPRALFLGRGRPLESVVDIAISKPRFRALAL
EEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEE
GEKRVWVMPGFTAADEQGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRV
CCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCE
VADAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQFHIPCYIKNSFNPDAPGTLVSNQA
EHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCEECCCC
DETGLQVKAISNLDNQTMFDISGPGMKGMVGMASRTLAAISRSGVSVSLITQSSSEYSIS
CCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
FCVATSDSAKVKWALEQEFELEIKSDLLEPIAMRHELAIVSLIGDGMRTHKGVAARFFQA
EEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LAQASVNIIAIAQGSSERSISTVIEQRKTKHAVAACHQGFFDVQQYLDVFLVGCGNVGAG
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHH
LLEQIKQQSAMLKEQHISIRVCGIVNSNKMLLDSAGIDLSNWSSLLADSQQACDIPALLA
HHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCCHHHHHHHHHCCHHHCCCHHHHH
WAKEQQLLNPVLVDCTSSEQVSDLYLDVMNSGLHVVTPNKKSNTRDYAYYQALRQTALKQ
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
RRQFLYETTVGAGLPVIDNLKKLLFAGDKLHKFNGILSGSLSFIFGMLDEGMTLSEATGL
HHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AREKCFTEPDPRDDLSGMDVARKVLILAREVGLKLELSDVQVESVLPAEFDDSGDVESFM
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCCCCCCCCHHHHH
ARLPQVDAAIAAKVAQAKAQGKVLRYVGQIEDGVCRVRIAEVDAQDPLYSVKGGENALAF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCHHHHH
YSRYYQPIPFVLRGYGAGTEVTAAGAFADVLRTLNWTREVSV
HHHHCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7003595; 1630901; 7610040; 9278503; 6277952; 2410621; 387092; 6298218; 390305 [H]