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The map label for this gene is rrmJ

Identifier: 120597892

GI number: 120597892

Start: 1206520

End: 1207149

Strand: Direct

Name: rrmJ

Synonym: Sputw3181_1062

Alternate gene names: 120597892

Gene position: 1206520-1207149 (Clockwise)

Preceding gene: 120597890

Following gene: 120597893

Centisome position: 25.62

GC content: 48.1

Gene sequence:

>630_bases
ATGTCAGGTAAAAAACGTACGGCCAGTTCCAATCGCTGGATGCTGGAACACTTTGATGATCATTATGTCAAACTAGCACA
GAAACGGGGATTGCGTTCTCGCGCTGCGTTTAAGCTTGAAGAACTTCAGCAAAAGGATCAACTTATTCGCCCTGGTATGA
CGGTAGTCGACCTGGGAGCAGCACCAGGTGGCTGGTCACAGGTAGCGGTTAAGTTGGTTGGAGATAGGGGAAAGGTTATC
GCCTGTGATATTTTACCCATGGATCCCATCGTTGGGGTAGACTTTTTGCAAGGGGATTTTCGTGAAGAAAAAGTCCTTGA
AGCATTGCTTACCCGCGTCGGTGCAGATAAGGTTGACGTTGTGTTGTCAGACATGGCGCCTAACATGAGTGGTTCAGACG
GTGTCGATCAACCGCGTGCTATGTATTTAGTGGAACTCGCCTTAGATATGTGTCATCAAGTATTGGCACCTAATGGGAGT
TTTGCGGTGAAAGTCTTTCAGGGGGAGGGCTTTGACGAATACATGAAAGCGGTTAAAGAAGCGTTTAAAGTAGTTAAAAC
ACGTAAACCGGACTCTTCGCGGGCGCGTTCCCGGGAAGTCTATCTTGTGGCGACAGGGTACAAGTTGTAG

Upstream 100 bases:

>100_bases
TTAAGTTCATTTCTACCAACTTTTGCTGAGTTACTGTTGAAAAACGGCTATTCTACCCTTATATAGCCCTTGTGTAACTC
TCAATTGTTGCGGATTTTAA

Downstream 100 bases:

>100_bases
TACTCTAGCGTTAATCATCGCAAGACTTTATGAGGTCTAGTAATTTGAGTGACATGGCAAAAAATCTAATACTCTGGGTT
GTCATCGCCGTTGTGCTGAT

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSGKKRTASSNRWMLEHFDDHYVKLAQKRGLRSRAAFKLEELQQKDQLIRPGMTVVDLGAAPGGWSQVAVKLVGDRGKVI
ACDILPMDPIVGVDFLQGDFREEKVLEALLTRVGADKVDVVLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGS
FAVKVFQGEGFDEYMKAVKEAFKVVKTRKPDSSRARSREVYLVATGYKL

Sequences:

>Translated_209_residues
MSGKKRTASSNRWMLEHFDDHYVKLAQKRGLRSRAAFKLEELQQKDQLIRPGMTVVDLGAAPGGWSQVAVKLVGDRGKVI
ACDILPMDPIVGVDFLQGDFREEKVLEALLTRVGADKVDVVLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGS
FAVKVFQGEGFDEYMKAVKEAFKVVKTRKPDSSRARSREVYLVATGYKL
>Mature_208_residues
SGKKRTASSNRWMLEHFDDHYVKLAQKRGLRSRAAFKLEELQQKDQLIRPGMTVVDLGAAPGGWSQVAVKLVGDRGKVIA
CDILPMDPIVGVDFLQGDFREEKVLEALLTRVGADKVDVVLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGSF
AVKVFQGEGFDEYMKAVKEAFKVVKTRKPDSSRARSREVYLVATGYKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=36.6197183098592, Blast_Score=144, Evalue=5e-35,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=36.1702127659575, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.7894736842105, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.7894736842105, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.7894736842105, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=72.5961538461538, Blast_Score=320, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=32.2727272727273, Blast_Score=113, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=34.3915343915344, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=34.3915343915344, Blast_Score=109, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=34.3915343915344, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=33.5078534031414, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=201, Percent_Identity=36.318407960199, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=33.1578947368421, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=279, Percent_Identity=27.5985663082437, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=37.7659574468085, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21356387, Length=225, Percent_Identity=35.1111111111111, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=34.3434343434343, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24647580, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23163; Mature: 23032

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKKRTASSNRWMLEHFDDHYVKLAQKRGLRSRAAFKLEELQQKDQLIRPGMTVVDLGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQVAVKLVGDRGKVIACDILPMDPIVGVDFLQGDFREEKVLEALLTRVGADKVDV
CCCCHHHHHHEEECCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCHHHH
VLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGSFAVKVFQGEGFDEYMKAVKE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFKVVKTRKPDSSRARSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SGKKRTASSNRWMLEHFDDHYVKLAQKRGLRSRAAFKLEELQQKDQLIRPGMTVVDLGA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQVAVKLVGDRGKVIACDILPMDPIVGVDFLQGDFREEKVLEALLTRVGADKVDV
CCCCHHHHHHEEECCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCHHHH
VLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGSFAVKVFQGEGFDEYMKAVKE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFKVVKTRKPDSSRARSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA