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The map label for this gene is carA [H]

Identifier: 120597832

GI number: 120597832

Start: 1144371

End: 1145531

Strand: Direct

Name: carA [H]

Synonym: Sputw3181_1002

Alternate gene names: 120597832

Gene position: 1144371-1145531 (Clockwise)

Preceding gene: 120597831

Following gene: 120597833

Centisome position: 24.3

GC content: 47.55

Gene sequence:

>1161_bases
ATGGAGGTCGCGTTGACAAAGTCTGCCTTACTCGTACTCGAAGATGGAACCGTATTCACTGGCACAGCAATTGGTGCCGA
TGGACTCTCTGTTGGTGAAGTTGTTTTTAACACTTCCATGACAGGTTACCAAGAAATTCTAACTGATCCATCCTACTCAC
GCCAGATTGTAACATTAACCTACCCCCACATTGGTAATACTGGTACCAATAATGAAGATATAGAATCCTATGGGGTTCAC
GCTTGTGGTCTTATCATTCGCGATCTTCCACTCATCGCAAGTAGTTTCCGCAATCAACAAAGCTTAAGTGATTATCTCAA
AGCGAACAATGTCGTTGGCATAGCAGATATCGATACCCGCAAGTTAACGCGTATTCTCCGTGAAAAAGGTGCCCAAGCCG
GTTGTATTATGGCTGGTGCAGACTTAGACGAAGCAAAAGCCCTTGACGCTGCCAAAGCTTTCCCCGGTTTAAAGGGTATG
GATCTCGCTAAAGAAGTCACGACTAAAACTGCCTATCCATGGCGTAAAGGTAGCTGGCGTTTAGTGGGCGGCTTACCTGA
AGATACGCCAGCAAATGAGCTTAAATACAAAGTAGTAGCCTACGACTACGGCGTTAAGCAAAACATTTTGCGTATGTTGG
TGGATCGCGGCTGCGACGTCACCGTGGTTCCTGCTAAAACCTCTGCCGCAGAAGTGTTAGCGATGAACCCAGATGGCGTA
TTCCTCTCGAATGGCCCTGGCGATCCAGAGCCATGTGATTACGCGATTGAAGCGATTCAGCAGATCCTGAAAACCGACAC
GCCAATTTTCGGTATCTGTTTAGGTCATCAGCTACTCGCGCTGGCCTCGGGTGCAAAAACCTTAAAGATGAAGTTTGGTC
ACCACGGTGCAAATCATCCTGTGAGCAATATCGAGCAAGGTAACGTGATGATCACTAGCCAAAACCATGGTTTTGCTGCC
GATGAAACGACTTTACCCGCCAACATTAAAGTGACCCATAAGTCGCTATTTGATGGTTCACTGCAAGGTATTCATCTGAC
GGACAAACCTGCGTTCAGCTTCCAAGGTCACCCTGAAGCGAGCCCTGGTCCGAACGATGCCGCCCCGCTGTTCGATCACT
TTATCGATCTAATCGACATATACCGTCAGAACGCTAAGTAA

Upstream 100 bases:

>100_bases
TCCTGTAAACTCGCTGGAATTTGTCAAAATTTCGTAAAACAGCGGAAATTGGTATCTTAAAACAGCATAAAAACATTATA
TTTCAGTGGCTTGGCTCTTT

Downstream 100 bases:

>100_bases
TACGCAAGCTAGGAGAAGAGAGAAGCAATGCCAAAACGTACAGATATAAAGAGTATTCTTATCCTAGGTGCAGGCCCGAT
TGTCATTGGTCAGGCATGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDIESYGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALDAAKAFPGLKGM
DLAKEVTTKTAYPWRKGSWRLVGGLPEDTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAA
DETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIDLIDIYRQNAK

Sequences:

>Translated_386_residues
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDIESYGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALDAAKAFPGLKGM
DLAKEVTTKTAYPWRKGSWRLVGGLPEDTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAA
DETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIDLIDIYRQNAK
>Mature_386_residues
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDIESYGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALDAAKAFPGLKGM
DLAKEVTTKTAYPWRKGSWRLVGGLPEDTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAA
DETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIDLIDIYRQNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=38.4816753926702, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI21361331, Length=384, Percent_Identity=36.9791666666667, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI169790915, Length=384, Percent_Identity=36.9791666666667, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=76.1780104712042, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=37.597911227154, Blast_Score=232, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=401, Percent_Identity=39.4014962593516, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=406, Percent_Identity=35.4679802955665, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=39.5348837209302, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45555749, Length=391, Percent_Identity=39.386189258312, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41439; Mature: 41439

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEEECEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNNEDIESYGVHACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALDAAKAFPGLKGMDLAKEVTTKTAYPWRKGSWR
HHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEE
LVGGLPEDTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
EECCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSNIEQGNVMITSQNHGFAADETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIDLIDIYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEEECEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNNEDIESYGVHACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALDAAKAFPGLKGMDLAKEVTTKTAYPWRKGSWR
HHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEE
LVGGLPEDTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
EECCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSNIEQGNVMITSQNHGFAADETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIDLIDIYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]