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The map label for this gene is rumB

Identifier: 120597721

GI number: 120597721

Start: 1011634

End: 1012803

Strand: Direct

Name: rumB

Synonym: Sputw3181_0891

Alternate gene names: 120597721

Gene position: 1011634-1012803 (Clockwise)

Preceding gene: 120597720

Following gene: 120597722

Centisome position: 21.49

GC content: 45.38

Gene sequence:

>1170_bases
GTGAGCGCTGTTATTGTAAATACCGTCGAGCAATGTGGTTATTTTAATCGAGGTCAGTGTCAATCCTGTCGCCATATTCA
AGTGCCTATGGCGCAACAGTTGATGGCTAAATCCCTAGAATTACAGCAATTACTCAAACCATTTGTGGCACCATCCGCAG
CTATTTTTTACCCTCCAGTGACTGGCGAGGCGACGGCTTTTCGCAATAAAGCCAAAATGGTGGTGTTAGGTGCGGCTCAT
GCACCTGTACTTGGGATTGTTAGTCCAAGTGGTGAGGCGGTAAGTCTTTGTGATTGCTTACTCTATCCATCTGATATGCA
AAAGTTATTGCACCGTTTAACGCAATTTGTACAGCAGGCGGGTATTCCGCCGTATCGTGTTGATAAAGCGAAGGGTGAGC
TTAAGTTTATTCTGCTAACGCGTAGCCAAGTTCGCGGCGAATATCTACTGCGTTTTGTATTGCGCTCGCACAATAGTATT
GAGCGTATCGAGAGAGCATTGCCTACATTACTGGCCGAATACCCACAAATTAACGTGGTTTCAGTCAATATTCAGCCTAT
TCATATGGCGATCCTTGAGGGGGATGAGGAAATTTTCTTAACTGAAAATACCCGCCTCGAAGAGCGTTTTAACGATGTGC
CTTTGTTTATTCGACCTAAAAGTTTTTTCCAAACCAATCCTCAAGTCGCTGCGCAGTTATACCAAACAGCCCGTGAGTGG
GTTGCTGAGTTTGCTCCTAAGTCGCTCTGGGATCTCTTTTGCGGAGTCGGTGGCTTTGGCTTGCACTGTGCGACTAAGGA
TATCGCGCTTACAGGCATTGAAATTGAAGCAGAGGCCATTAGTTGTGCACAAATATCGGCTAACTTGATGGGGCTTGAGA
AGGTGCAATTTATGGCGCTTGATTCAACTGATTTTGCTCAGGGCAAAAACGCCGCCGATAAGCCCGATTTGATTATTGTC
AATCCGCCACGGCGCGGGATTGGAGAGGCATTGTGTCAATCCTTAAGCGAGTTTGCGCCTAGGGCGATTCTTTATTCCAG
TTGTAACCCAAAGACCTTAGCTAAAGATCTCGAGCACATTCAAGGTTATCACTTAACTAAAGTGCAACTGTTTGATTTAT
TTCCGCACACAGATCACTTCGAAGTATTAGCCATGTTAGTGAAGGATTAA

Upstream 100 bases:

>100_bases
GTTGACTCACTCACTTTGAGCTGAATCGCGTGACTTTGGGTGATTTTTACCTGTAAAAACGCTACAGTGTGGCGCTTTTT
ATTGATATCTAGGAACTGCT

Downstream 100 bases:

>100_bases
CAGTATCAATGGTGCTGGTTGTCGGTGACGTCAGTGGCATCTTTTATGTATTTCATCAAAATGTCTTTTTAGTGACATAC
TCTTTAATACGTTAGGTCAA

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSAVIVNTVEQCGYFNRGQCQSCRHIQVPMAQQLMAKSLELQQLLKPFVAPSAAIFYPPVTGEATAFRNKAKMVVLGAAH
APVLGIVSPSGEAVSLCDCLLYPSDMQKLLHRLTQFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNSI
ERIERALPTLLAEYPQINVVSVNIQPIHMAILEGDEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAQLYQTAREW
VAEFAPKSLWDLFCGVGGFGLHCATKDIALTGIEIEAEAISCAQISANLMGLEKVQFMALDSTDFAQGKNAADKPDLIIV
NPPRRGIGEALCQSLSEFAPRAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHTDHFEVLAMLVKD

Sequences:

>Translated_389_residues
MSAVIVNTVEQCGYFNRGQCQSCRHIQVPMAQQLMAKSLELQQLLKPFVAPSAAIFYPPVTGEATAFRNKAKMVVLGAAH
APVLGIVSPSGEAVSLCDCLLYPSDMQKLLHRLTQFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNSI
ERIERALPTLLAEYPQINVVSVNIQPIHMAILEGDEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAQLYQTAREW
VAEFAPKSLWDLFCGVGGFGLHCATKDIALTGIEIEAEAISCAQISANLMGLEKVQFMALDSTDFAQGKNAADKPDLIIV
NPPRRGIGEALCQSLSEFAPRAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHTDHFEVLAMLVKD
>Mature_388_residues
SAVIVNTVEQCGYFNRGQCQSCRHIQVPMAQQLMAKSLELQQLLKPFVAPSAAIFYPPVTGEATAFRNKAKMVVLGAAHA
PVLGIVSPSGEAVSLCDCLLYPSDMQKLLHRLTQFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNSIE
RIERALPTLLAEYPQINVVSVNIQPIHMAILEGDEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAQLYQTAREWV
AEFAPKSLWDLFCGVGGFGLHCATKDIALTGIEIEAEAISCAQISANLMGLEKVQFMALDSTDFAQGKNAADKPDLIIVN
PPRRGIGEALCQSLSEFAPRAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHTDHFEVLAMLVKD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI51173878, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI269784680, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI269784676, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI20149688, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI269784678, Length=190, Percent_Identity=27.8947368421053, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787083, Length=378, Percent_Identity=54.2328042328042, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1789148, Length=184, Percent_Identity=32.6086956521739, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=23.696682464455, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 43300; Mature: 43169

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVIVNTVEQCGYFNRGQCQSCRHIQVPMAQQLMAKSLELQQLLKPFVAPSAAIFYPPV
CCEEEEEHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
TGEATAFRNKAKMVVLGAAHAPVLGIVSPSGEAVSLCDCLLYPSDMQKLLHRLTQFVQQA
CCCHHHHCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GIPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNSIERIERALPTLLAEYPQINVV
CCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEE
SVNIQPIHMAILEGDEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAQLYQTAREW
EEEEEEEEEEEEECCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHH
VAEFAPKSLWDLFCGVGGFGLHCATKDIALTGIEIEAEAISCAQISANLMGLEKVQFMAL
HHHHCCHHHHHHHHCCCCCCEEEECCCEEEEEEEEEHHHHHHHHHHHHHHCHHHEEEEEE
DSTDFAQGKNAADKPDLIIVNPPRRGIGEALCQSLSEFAPRAILYSSCNPKTLAKDLEHI
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
QGYHLTKVQLFDLFPHTDHFEVLAMLVKD
CCCCEEEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAVIVNTVEQCGYFNRGQCQSCRHIQVPMAQQLMAKSLELQQLLKPFVAPSAAIFYPPV
CEEEEEHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
TGEATAFRNKAKMVVLGAAHAPVLGIVSPSGEAVSLCDCLLYPSDMQKLLHRLTQFVQQA
CCCHHHHCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GIPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNSIERIERALPTLLAEYPQINVV
CCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEE
SVNIQPIHMAILEGDEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAQLYQTAREW
EEEEEEEEEEEEECCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHH
VAEFAPKSLWDLFCGVGGFGLHCATKDIALTGIEIEAEAISCAQISANLMGLEKVQFMAL
HHHHCCHHHHHHHHCCCCCCEEEECCCEEEEEEEEEHHHHHHHHHHHHHHCHHHEEEEEE
DSTDFAQGKNAADKPDLIIVNPPRRGIGEALCQSLSEFAPRAILYSSCNPKTLAKDLEHI
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
QGYHLTKVQLFDLFPHTDHFEVLAMLVKD
CCCCEEEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA