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The map label for this gene is rpfG [H]

Identifier: 120597633

GI number: 120597633

Start: 906688

End: 907704

Strand: Direct

Name: rpfG [H]

Synonym: Sputw3181_0802

Alternate gene names: 120597633

Gene position: 906688-907704 (Clockwise)

Preceding gene: 120597632

Following gene: 120597636

Centisome position: 19.26

GC content: 44.15

Gene sequence:

>1017_bases
ATGGATAAGGCAACAATATTAGTCGTTGATGATACTCCCGAGAATATCGACATCTTAGTGGGTATTCTTGGAGAAGATTA
TAAAGTCAAAGTGGCTATTGATGGACCTAGGGCGTTGGCACTTGTCGCTAAATCCTTGCCGGATCTCATTTTATTGGATG
TAATGATGCCGGGAATGAATGGTTATGAGGTTTGTAAGTTATTAAAACAAGAGCCACTGACATGCCATATCCCCGTTATT
TTTGTTACCGCATTATCTGAAGTGGCTGATGAAACCCAAGGTTTTGAGTTGGGTGCAGTCGATTACATTACTAAGCCTGT
CAGTGCGCCAGTTGTTAAGGCGCGAGTTAAAACGCATCTTGCATTGTATGATCAAAAGCGACTTTTAGAACAGCAAGTTA
AAGAAAGAACACAAGAGCTAGAAGATACGCGTTTCGAAATTATTCGTCGCCTTGGCCGTGCCGCAGAATATAAAGATAAT
GAAACAGGCTTACATGTGGTACGTATGAGTCACTATGCGCGGTTACTTGCGATAAGGCTTGGTTTACCAACGACTTTTTG
CGACTTGCTTTATAATGCAGCACCGATGCATGATATAGGCAAAATAGGTACACCCGATGCCGTATTAAAAAAACCGGGTA
AGCTAGACCCTGAAGAATGGGAAATTATGCAACAGCACGCCGCCATAGGTGCAGAAATTATCGGTGAGCATAGCGACCCT
CTATTACAAATGGCCCGTCGTATCGCCTTGACTCACCACGAAAAATGGGATGGTTCAGGCTACCCCAATGGGTTAATGGG
TGAGAATATCCCAATTGAAGGCCGTATCACCGCGATTGCGGACGTGTTCGATGCACTAACATCCCAGCGACCGTATAAAC
AGCCTTGGACCATAGAGGCAACAATGGAGTTACTCGAAAACCAAGCCGGTAAGCATTTTGACCCTAAGCTGGTGGAGGCA
TTTAAACAAATCCTGCCAGAAGTCATTGCGATTCGAGATACCTATTTAGAGCATTGA

Upstream 100 bases:

>100_bases
ACGTGTCGAATATGTGCCTGTGTTTGATATCGGCTATGGAATAGCGTTGGTAAAAGAGCGGACAAAATAGTTTGTTATTT
GGCATAAGTCGAGGTTGTGA

Downstream 100 bases:

>100_bases
GGATTAAATCTTAGTGAAAATAAAGGCGCACATGGCGCCTTTATCGTTTATAAAATAAAGGGTTATGCTTCATCTAGTGA
GTAAGGTAAGGCTTGAATTG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MDKATILVVDDTPENIDILVGILGEDYKVKVAIDGPRALALVAKSLPDLILLDVMMPGMNGYEVCKLLKQEPLTCHIPVI
FVTALSEVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKERTQELEDTRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAIRLGLPTTFCDLLYNAAPMHDIGKIGTPDAVLKKPGKLDPEEWEIMQQHAAIGAEIIGEHSDP
LLQMARRIALTHHEKWDGSGYPNGLMGENIPIEGRITAIADVFDALTSQRPYKQPWTIEATMELLENQAGKHFDPKLVEA
FKQILPEVIAIRDTYLEH

Sequences:

>Translated_338_residues
MDKATILVVDDTPENIDILVGILGEDYKVKVAIDGPRALALVAKSLPDLILLDVMMPGMNGYEVCKLLKQEPLTCHIPVI
FVTALSEVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKERTQELEDTRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAIRLGLPTTFCDLLYNAAPMHDIGKIGTPDAVLKKPGKLDPEEWEIMQQHAAIGAEIIGEHSDP
LLQMARRIALTHHEKWDGSGYPNGLMGENIPIEGRITAIADVFDALTSQRPYKQPWTIEATMELLENQAGKHFDPKLVEA
FKQILPEVIAIRDTYLEH
>Mature_338_residues
MDKATILVVDDTPENIDILVGILGEDYKVKVAIDGPRALALVAKSLPDLILLDVMMPGMNGYEVCKLLKQEPLTCHIPVI
FVTALSEVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKERTQELEDTRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAIRLGLPTTFCDLLYNAAPMHDIGKIGTPDAVLKKPGKLDPEEWEIMQQHAAIGAEIIGEHSDP
LLQMARRIALTHHEKWDGSGYPNGLMGENIPIEGRITAIADVFDALTSQRPYKQPWTIEATMELLENQAGKHFDPKLVEA
FKQILPEVIAIRDTYLEH

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=142, Percent_Identity=38.0281690140845, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=39.8373983739837, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=40.7079646017699, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=126, Percent_Identity=38.0952380952381, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=35.3448275862069, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=31.8965517241379, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=37.1900826446281, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=106, Percent_Identity=38.6792452830189, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=33.6206896551724, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37838; Mature: 37838

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKATILVVDDTPENIDILVGILGEDYKVKVAIDGPRALALVAKSLPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCC
GYEVCKLLKQEPLTCHIPVIFVTALSEVADETQGFELGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHH
ALYDQKRLLEQQVKERTQELEDTRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLAIRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
GLPTTFCDLLYNAAPMHDIGKIGTPDAVLKKPGKLDPEEWEIMQQHAAIGAEIIGEHSDP
CCCHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
LLQMARRIALTHHEKWDGSGYPNGLMGENIPIEGRITAIADVFDALTSQRPYKQPWTIEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH
TMELLENQAGKHFDPKLVEAFKQILPEVIAIRDTYLEH
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKATILVVDDTPENIDILVGILGEDYKVKVAIDGPRALALVAKSLPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCC
GYEVCKLLKQEPLTCHIPVIFVTALSEVADETQGFELGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHH
ALYDQKRLLEQQVKERTQELEDTRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLAIRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
GLPTTFCDLLYNAAPMHDIGKIGTPDAVLKKPGKLDPEEWEIMQQHAAIGAEIIGEHSDP
CCCHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
LLQMARRIALTHHEKWDGSGYPNGLMGENIPIEGRITAIADVFDALTSQRPYKQPWTIEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH
TMELLENQAGKHFDPKLVEAFKQILPEVIAIRDTYLEH
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]