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The map label for this gene is frdA [H]

Identifier: 120597331

GI number: 120597331

Start: 538022

End: 540028

Strand: Direct

Name: frdA [H]

Synonym: Sputw3181_0500

Alternate gene names: 120597331

Gene position: 538022-540028 (Clockwise)

Preceding gene: 120597330

Following gene: 120597332

Centisome position: 11.43

GC content: 48.73

Gene sequence:

>2007_bases
GTGAAACTGATTTATACCGATTCATTAGTGGTAGGAGCCGGGCTTGCCGGTTTAAGGGTCGCCATTGCATCGAAAGAGCG
TGGGCTGGATACCTTAGTTCTATCGCTTATTCCTGCGAAACGTTCACACTCGGCGGCGGCACAAGGGGGAATGCAGGCGA
GTTTAGGGAATGCCGTTAAGGGGATGGGTGATGATGAAGATGTCCACTTTCAAGACACTGTAAAAGGTTCCGATTGGGGC
TGCGATCAAGAAGTGGCGCGTATGTTTGCTCATTGTGCACCTAAGGCCGTGCGTGAATTAGCAAATTGGGGCGTGCCTTG
GTCAAGGGTGAGTGCTGGCCCGAGAGAAGTGATAGTCAATGCGCAAAAGGTCACTCTCCATGAGGCCGAGGAAGCTCATG
GTCTTATCAACGCCCGTGATTTTGGCGGGACTAAAAAGTGGCGAACCTGTTACACCGCAGATGGGACTGGTCACTCATTA
TTGTATGCGATGGACAACAAAGCGATTTCTATGGACATTCCCGTCCATGAACGTGTTGAAGCCTTGGCGCTTATTCACGA
TGGCAAACGCTGTCACGGCGTAGTGGCTCGTTGTTTGATTACGGGTGAACTGCGTGCTTATGTGGCTAAATCCACCACCA
TTGCTACTGGTGGTTATGGCCGTATCTATGAAGTATCAACAAACGCCATTATCTGTGAAGGTGTAGGGCACGCGCTCGCT
TTAGAAACTGGTGTTGCGACCTTAGGTAATATGGAGGCCGTGCAATTCCATCCGACGGCTATTGTACCTGTGGGCATTCT
CACGACAGAGGGATGCCGTGGTGATGGTGGCTTATTGCGCGATAAAGATGGTTATCGCTTTATGCCAGACTATGAACCCG
ATAAAAAAGAGTTGGCATCGCGGGACGTAGTATCACGCCGTATGACAGAGCATATGCGTAAAGGCAAAGGGGTTGACAGT
CCTTATGGACCACATTTATGGCTCGATATTACCCTCTTAGGTCGTAAACATATTGAAACAAACCTTCGGGAAGTACAGGA
AATTTGTGAGAACTTTCTGGGTATTGATCCCGCTAAAGATTGGATTCCTGTACGCCCAACTCAGCATTATTCTATGGGCG
GTATTCGGACTAATGCCACAGGTGAAAGTCCACAGCTTAAAGGGTTATTTAGCGTGGGTGAGGCGGCTTGTTGGGATATG
CACGGCTTTAACCGTTTAGGCGGTAATTCGCTAGCCGAAACTGTGGTCGGGGGCATGATTATCGGTAAATATGTTGCTGA
TTTTTGTGAAAATAACAGTTTAGAAATCAATACCCAACTTGCTGAAAAGTTTATGAAGCAAGTGCAAGATGAAATCGATA
CGTTAATCGAAGGGGACGGCACAGAGAGCCCCTTCGAGCTTAAGCATGAAATGCAGCGTATTATGATGGATTACGTGGGG
ATATTCCGTAATGGCCCCGAGCTTGAAAAAGCGGTTGCCGAGCTTAAAGTCTTGCTCGAGCGTTCACGTAAACTCGGGCT
TAAGTGTAAAAAACGTCACGCTAATCCTGAGTTAGTCGAAGCATTACGGGTCAAGCGGATGTTGAAAGTCGCGTTAACCG
TAGCCTGCGGCGCAGCAGCGCGTACCGAAAGCCGCGGCGCCCATGCTCGCGAGGATTACCCGCAGCGAAATGACCGAGAT
TGGCTGAATCGCACGTTGGCAAGCTGGCCTGATCCCAATGCACTTGAACCCATGCTTAATTATGAAGCCCTCGATGTGAT
GAAGATGGAATTACCGCCTGGCTATCGCGGTTATGGGATCGACAACGCCATAGTGCATCCCGATACCCAAAAACGTGAGC
AGCAAATCGCTGAGATTTTAGCAGGGCTGGGTGATGATACAGATCGCTATCAAAGGCAGCGAGCCCTTATGCCATTTGAG
TTACCCGAGAGTTTGCAACCTAATAATGAACGTTTAAGTGACACGTTAAAAAAACCATCAGCCAATGCGTTAGGAGAAAA
ATCATAA

Upstream 100 bases:

>100_bases
CCTCACGCAAGTTAAATAAATTCTAAGAAAAACATAGTTGTAGTTGATGTGTTATGAGAGAAAACCCGACAATAATAGCA
AATCCCGTTAAGGAGCAGGC

Downstream 100 bases:

>100_bases
TGAGCCAAGGTCGCCAGTTAACCTTTAATATCTTTCGTTATGATCCGCAGGAGCCAAACGATAAGCCGAAAATGGTACGT
TATCAGATCACCGAAACACC

Product: fumarate reductase flavoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKLIYTDSLVVGAGLAGLRVAIASKERGLDTLVLSLIPAKRSHSAAAQGGMQASLGNAVKGMGDDEDVHFQDTVKGSDWG
CDQEVARMFAHCAPKAVRELANWGVPWSRVSAGPREVIVNAQKVTLHEAEEAHGLINARDFGGTKKWRTCYTADGTGHSL
LYAMDNKAISMDIPVHERVEALALIHDGKRCHGVVARCLITGELRAYVAKSTTIATGGYGRIYEVSTNAIICEGVGHALA
LETGVATLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGYRFMPDYEPDKKELASRDVVSRRMTEHMRKGKGVDS
PYGPHLWLDITLLGRKHIETNLREVQEICENFLGIDPAKDWIPVRPTQHYSMGGIRTNATGESPQLKGLFSVGEAACWDM
HGFNRLGGNSLAETVVGGMIIGKYVADFCENNSLEINTQLAEKFMKQVQDEIDTLIEGDGTESPFELKHEMQRIMMDYVG
IFRNGPELEKAVAELKVLLERSRKLGLKCKKRHANPELVEALRVKRMLKVALTVACGAAARTESRGAHAREDYPQRNDRD
WLNRTLASWPDPNALEPMLNYEALDVMKMELPPGYRGYGIDNAIVHPDTQKREQQIAEILAGLGDDTDRYQRQRALMPFE
LPESLQPNNERLSDTLKKPSANALGEKS

Sequences:

>Translated_668_residues
MKLIYTDSLVVGAGLAGLRVAIASKERGLDTLVLSLIPAKRSHSAAAQGGMQASLGNAVKGMGDDEDVHFQDTVKGSDWG
CDQEVARMFAHCAPKAVRELANWGVPWSRVSAGPREVIVNAQKVTLHEAEEAHGLINARDFGGTKKWRTCYTADGTGHSL
LYAMDNKAISMDIPVHERVEALALIHDGKRCHGVVARCLITGELRAYVAKSTTIATGGYGRIYEVSTNAIICEGVGHALA
LETGVATLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGYRFMPDYEPDKKELASRDVVSRRMTEHMRKGKGVDS
PYGPHLWLDITLLGRKHIETNLREVQEICENFLGIDPAKDWIPVRPTQHYSMGGIRTNATGESPQLKGLFSVGEAACWDM
HGFNRLGGNSLAETVVGGMIIGKYVADFCENNSLEINTQLAEKFMKQVQDEIDTLIEGDGTESPFELKHEMQRIMMDYVG
IFRNGPELEKAVAELKVLLERSRKLGLKCKKRHANPELVEALRVKRMLKVALTVACGAAARTESRGAHAREDYPQRNDRD
WLNRTLASWPDPNALEPMLNYEALDVMKMELPPGYRGYGIDNAIVHPDTQKREQQIAEILAGLGDDTDRYQRQRALMPFE
LPESLQPNNERLSDTLKKPSANALGEKS
>Mature_668_residues
MKLIYTDSLVVGAGLAGLRVAIASKERGLDTLVLSLIPAKRSHSAAAQGGMQASLGNAVKGMGDDEDVHFQDTVKGSDWG
CDQEVARMFAHCAPKAVRELANWGVPWSRVSAGPREVIVNAQKVTLHEAEEAHGLINARDFGGTKKWRTCYTADGTGHSL
LYAMDNKAISMDIPVHERVEALALIHDGKRCHGVVARCLITGELRAYVAKSTTIATGGYGRIYEVSTNAIICEGVGHALA
LETGVATLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGYRFMPDYEPDKKELASRDVVSRRMTEHMRKGKGVDS
PYGPHLWLDITLLGRKHIETNLREVQEICENFLGIDPAKDWIPVRPTQHYSMGGIRTNATGESPQLKGLFSVGEAACWDM
HGFNRLGGNSLAETVVGGMIIGKYVADFCENNSLEINTQLAEKFMKQVQDEIDTLIEGDGTESPFELKHEMQRIMMDYVG
IFRNGPELEKAVAELKVLLERSRKLGLKCKKRHANPELVEALRVKRMLKVALTVACGAAARTESRGAHAREDYPQRNDRD
WLNRTLASWPDPNALEPMLNYEALDVMKMELPPGYRGYGIDNAIVHPDTQKREQQIAEILAGLGDDTDRYQRQRALMPFE
LPESLQPNNERLSDTLKKPSANALGEKS

Specific function: The fumarate reductase enzyme complex is required for fumarate respiration using formate or sulfide as electron donor [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=544, Percent_Identity=37.8676470588235, Blast_Score=325, Evalue=1e-88,
Organism=Escherichia coli, GI1786942, Length=604, Percent_Identity=36.2582781456954, Blast_Score=329, Evalue=4e-91,
Organism=Escherichia coli, GI1790597, Length=587, Percent_Identity=36.4565587734242, Blast_Score=318, Evalue=9e-88,
Organism=Escherichia coli, GI1788928, Length=568, Percent_Identity=29.4014084507042, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17505833, Length=550, Percent_Identity=38.5454545454545, Blast_Score=328, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17550100, Length=545, Percent_Identity=38.5321100917431, Blast_Score=314, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6322701, Length=597, Percent_Identity=36.6834170854271, Blast_Score=328, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6322416, Length=593, Percent_Identity=36.256323777403, Blast_Score=319, Evalue=7e-88,
Organism=Drosophila melanogaster, GI17137288, Length=603, Percent_Identity=36.318407960199, Blast_Score=328, Evalue=8e-90,
Organism=Drosophila melanogaster, GI24655642, Length=603, Percent_Identity=36.318407960199, Blast_Score=328, Evalue=8e-90,
Organism=Drosophila melanogaster, GI24655647, Length=603, Percent_Identity=36.318407960199, Blast_Score=328, Evalue=8e-90,
Organism=Drosophila melanogaster, GI24663005, Length=571, Percent_Identity=35.0262697022767, Blast_Score=284, Evalue=1e-76,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 73587; Mature: 73587

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00504 FRD_SDH_FAD_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIYTDSLVVGAGLAGLRVAIASKERGLDTLVLSLIPAKRSHSAAAQGGMQASLGNAVK
CEEEEECCHHHHCCHHHHEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHC
GMGDDEDVHFQDTVKGSDWGCDQEVARMFAHCAPKAVRELANWGVPWSRVSAGPREVIVN
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCHHHHCCCCHHHEEE
AQKVTLHEAEEAHGLINARDFGGTKKWRTCYTADGTGHSLLYAMDNKAISMDIPVHERVE
CHHEEEHHHHHHCCCEECCCCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCHHHHHH
ALALIHDGKRCHGVVARCLITGELRAYVAKSTTIATGGYGRIYEVSTNAIICEGVGHALA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCEEEECCCCCHHH
LETGVATLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGYRFMPDYEPDKKELAS
HHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
RDVVSRRMTEHMRKGKGVDSPYGPHLWLDITLLGRKHIETNLREVQEICENFLGIDPAKD
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
WIPVRPTQHYSMGGIRTNATGESPQLKGLFSVGEAACWDMHGFNRLGGNSLAETVVGGMI
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCHHHHCCCCCHHCCCHHHHHHHHHHHH
IGKYVADFCENNSLEINTQLAEKFMKQVQDEIDTLIEGDGTESPFELKHEMQRIMMDYVG
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IFRNGPELEKAVAELKVLLERSRKLGLKCKKRHANPELVEALRVKRMLKVALTVACGAAA
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
RTESRGAHAREDYPQRNDRDWLNRTLASWPDPNALEPMLNYEALDVMKMELPPGYRGYGI
CCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCHHHHEEEEECCCCCCCCCC
DNAIVHPDTQKREQQIAEILAGLGDDTDRYQRQRALMPFELPESLQPNNERLSDTLKKPS
CCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
ANALGEKS
CCCCCCCC
>Mature Secondary Structure
MKLIYTDSLVVGAGLAGLRVAIASKERGLDTLVLSLIPAKRSHSAAAQGGMQASLGNAVK
CEEEEECCHHHHCCHHHHEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHC
GMGDDEDVHFQDTVKGSDWGCDQEVARMFAHCAPKAVRELANWGVPWSRVSAGPREVIVN
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCHHHHCCCCHHHEEE
AQKVTLHEAEEAHGLINARDFGGTKKWRTCYTADGTGHSLLYAMDNKAISMDIPVHERVE
CHHEEEHHHHHHCCCEECCCCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCHHHHHH
ALALIHDGKRCHGVVARCLITGELRAYVAKSTTIATGGYGRIYEVSTNAIICEGVGHALA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCEEEECCCCCHHH
LETGVATLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGYRFMPDYEPDKKELAS
HHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
RDVVSRRMTEHMRKGKGVDSPYGPHLWLDITLLGRKHIETNLREVQEICENFLGIDPAKD
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
WIPVRPTQHYSMGGIRTNATGESPQLKGLFSVGEAACWDMHGFNRLGGNSLAETVVGGMI
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCHHHHCCCCCHHCCCHHHHHHHHHHHH
IGKYVADFCENNSLEINTQLAEKFMKQVQDEIDTLIEGDGTESPFELKHEMQRIMMDYVG
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IFRNGPELEKAVAELKVLLERSRKLGLKCKKRHANPELVEALRVKRMLKVALTVACGAAA
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
RTESRGAHAREDYPQRNDRDWLNRTLASWPDPNALEPMLNYEALDVMKMELPPGYRGYGI
CCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCHHHHEEEEECCCCCCCCCC
DNAIVHPDTQKREQQIAEILAGLGDDTDRYQRQRALMPFELPESLQPNNERLSDTLKKPS
CCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
ANALGEKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2244791; 9492313; 14500908; 3609021; 10586875; 11248702 [H]