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The map label for this gene is murE

Identifier: 120597216

GI number: 120597216

Start: 415496

End: 416998

Strand: Direct

Name: murE

Synonym: Sputw3181_0385

Alternate gene names: 120597216

Gene position: 415496-416998 (Clockwise)

Preceding gene: 120597215

Following gene: 120597217

Centisome position: 8.82

GC content: 49.43

Gene sequence:

>1503_bases
ATGATGTTGCTACGGGACCTGTTAGCGCCTTGGTTTATATATTCGGGTGATCAGTCTTTCAATGAGTTGACCTTAGATAG
CCGCGCAATACGCCGCGGCGATGTGTTTTTGGCGTTGCCAGGGTATAGGGTCGATGGCAGACAGTTTATTGATAAAGCCT
TAGATTTAGGTGCCGCAGGCGTATTAGTTCACACGGATGATGCTGACCAACATGGCAAAGTGTTGCTTGGCGATAATGCT
GAGCGTGGTGTGCAGATTTATTTTTTCCAATTAAATCGTCAGGTATCAGCGATAGCAGCCCAGCGTTATCCCTTAGCGCA
CCAGTTTGCCGTTACGCCATCCCTTGGCATTATCGGCATTACCGGCACCAATGGTAAAACCTCAACTAGTCAACTCATTG
CACAGCTAGTGACGTCATTGAGGCGAAAAGCTGCTGTTATGGGTACATTGGGAAATGGCCTATGGGGTGAACTAGTTGAT
AGCGGTAATACCACTGCCGATGCGATTACCCTAATGCGTCAGTTGCATGAATTTGAAGCCCAAGGCGCGAGCGTTTGTGC
TATGGAAGTGTCGAGCCACGGGCTAGTGCAAGGCCGCGTCGATGCAGTACCATTCGATGTCGCCGTGTTCACTAACTTAA
CGCGCGATCACCTCGATTACCACGGCGACATGGAAAGCTATGCCACCGCTAAGCAGAGCCTATTTCGTTTCAGTACCTTA
GGTCACGGTTTATTGAATCTCGATGACCCCGTTGGTGCATCTTGGTTGATTGAGCTTAAAGACATGGCTGCACAGATCTG
GGGCTTTAGTATCGAAGGCCACCAAGCCGCCGCTTTTTATACCAAAAATACTCAGTTTGACGATCAAGGCGTAAGTACAA
CTTTAGTGTGGCCCGAGGGCGAAACTCAAATTCACTCACCACTTCTTGGTGCCTTTAATCTCTCTAATTTACTCGCGGCG
TTATCGGCCTTGTATCTGCAAGGTTTTGATATGGAAGCCTTAGCCGAGCAAGTGCAATATTTAACGCCTGTTGCGGGTCG
TATGGAGCGTTTTACTACGGCGGATAACATCACCTTAGTCGTCGATTATGCACATACACCCGATGCGATAGAGCAGGCAT
TAACGGCCTTGCGCCGCCATTGTGCGGGGGATTTATGGTGCGTGTTTGGTTGCGGTGGAGACCGCGATAAGGGCAAGCGC
CCTTTGATGGGGCGTGCCGCTGAGCAATATGCAGACCGTATTATGGTGACCAGTGATAATGCACGCAGCGAAGATCCTGC
ACAAATTATTAGCGATATTATCCAAGGGCTTGTCACTCCAGAACGAGCATTGACTCAGGTTGACCGTATCACAGCAATTA
AAGACGTTGTGGGATTGGCTAAATCTGGCGATCTGATCTTACTTGCGGGCAAAGGCCACGAAACTTACCAAGAGGCGGCA
GGCGTGCGTCATAACTATGATGAACGCGCTTTGGCACGCCAGTTAGCGGAGCAATCAAAATGA

Upstream 100 bases:

>100_bases
CATTTGCCAAGGTAATGTCTGGCGCACTGCAAATGTTAAATGTCGAACCTATTTCCGATAAAGAACAGGTGCAATTGGCC
GGAATTGCAGGGAGAGCAGA

Downstream 100 bases:

>100_bases
TCCCTCTTTCACTCGAAGTGCTAAGTCAACATTTAGGTGCGCATCGAGTCGGTAACGACGTTACCATCCAAGATTTAAGC
AGTGATAGCCGCAAAGTCGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MMLLRDLLAPWFIYSGDQSFNELTLDSRAIRRGDVFLALPGYRVDGRQFIDKALDLGAAGVLVHTDDADQHGKVLLGDNA
ERGVQIYFFQLNRQVSAIAAQRYPLAHQFAVTPSLGIIGITGTNGKTSTSQLIAQLVTSLRRKAAVMGTLGNGLWGELVD
SGNTTADAITLMRQLHEFEAQGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYATAKQSLFRFSTL
GHGLLNLDDPVGASWLIELKDMAAQIWGFSIEGHQAAAFYTKNTQFDDQGVSTTLVWPEGETQIHSPLLGAFNLSNLLAA
LSALYLQGFDMEALAEQVQYLTPVAGRMERFTTADNITLVVDYAHTPDAIEQALTALRRHCAGDLWCVFGCGGDRDKGKR
PLMGRAAEQYADRIMVTSDNARSEDPAQIISDIIQGLVTPERALTQVDRITAIKDVVGLAKSGDLILLAGKGHETYQEAA
GVRHNYDERALARQLAEQSK

Sequences:

>Translated_500_residues
MMLLRDLLAPWFIYSGDQSFNELTLDSRAIRRGDVFLALPGYRVDGRQFIDKALDLGAAGVLVHTDDADQHGKVLLGDNA
ERGVQIYFFQLNRQVSAIAAQRYPLAHQFAVTPSLGIIGITGTNGKTSTSQLIAQLVTSLRRKAAVMGTLGNGLWGELVD
SGNTTADAITLMRQLHEFEAQGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYATAKQSLFRFSTL
GHGLLNLDDPVGASWLIELKDMAAQIWGFSIEGHQAAAFYTKNTQFDDQGVSTTLVWPEGETQIHSPLLGAFNLSNLLAA
LSALYLQGFDMEALAEQVQYLTPVAGRMERFTTADNITLVVDYAHTPDAIEQALTALRRHCAGDLWCVFGCGGDRDKGKR
PLMGRAAEQYADRIMVTSDNARSEDPAQIISDIIQGLVTPERALTQVDRITAIKDVVGLAKSGDLILLAGKGHETYQEAA
GVRHNYDERALARQLAEQSK
>Mature_500_residues
MMLLRDLLAPWFIYSGDQSFNELTLDSRAIRRGDVFLALPGYRVDGRQFIDKALDLGAAGVLVHTDDADQHGKVLLGDNA
ERGVQIYFFQLNRQVSAIAAQRYPLAHQFAVTPSLGIIGITGTNGKTSTSQLIAQLVTSLRRKAAVMGTLGNGLWGELVD
SGNTTADAITLMRQLHEFEAQGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYATAKQSLFRFSTL
GHGLLNLDDPVGASWLIELKDMAAQIWGFSIEGHQAAAFYTKNTQFDDQGVSTTLVWPEGETQIHSPLLGAFNLSNLLAA
LSALYLQGFDMEALAEQVQYLTPVAGRMERFTTADNITLVVDYAHTPDAIEQALTALRRHCAGDLWCVFGCGGDRDKGKR
PLMGRAAEQYADRIMVTSDNARSEDPAQIISDIIQGLVTPERALTQVDRITAIKDVVGLAKSGDLILLAGKGHETYQEAA
GVRHNYDERALARQLAEQSK

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=498, Percent_Identity=45.9839357429719, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1786274, Length=350, Percent_Identity=24.8571428571429, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 54526; Mature: 54526

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLLRDLLAPWFIYSGDQSFNELTLDSRAIRRGDVFLALPGYRVDGRQFIDKALDLGAAG
CHHHHHHHHHHEEECCCCCCCCEECCHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCE
VLVHTDDADQHGKVLLGDNAERGVQIYFFQLNRQVSAIAAQRYPLAHQFAVTPSLGIIGI
EEEECCCCCCCCCEEECCCCCCCEEEEEEEECCHHHHHHHHCCCCHHEEEECCCCCEEEE
TGTNGKTSTSQLIAQLVTSLRRKAAVMGTLGNGLWGELVDSGNTTADAITLMRQLHEFEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHC
QGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYATAKQSLFRFSTL
CCCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GHGLLNLDDPVGASWLIELKDMAAQIWGFSIEGHQAAAFYTKNTQFDDQGVSTTLVWPEG
CCCCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCCEEEEEECCCCCCCCCCEEEEECCCC
ETQIHSPLLGAFNLSNLLAALSALYLQGFDMEALAEQVQYLTPVAGRMERFTTADNITLV
CCHHCCCHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VDYAHTPDAIEQALTALRRHCAGDLWCVFGCGGDRDKGKRPLMGRAAEQYADRIMVTSDN
EEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCEEEEECCC
ARSEDPAQIISDIIQGLVTPERALTQVDRITAIKDVVGLAKSGDLILLAGKGHETYQEAA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHC
GVRHNYDERALARQLAEQSK
CCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMLLRDLLAPWFIYSGDQSFNELTLDSRAIRRGDVFLALPGYRVDGRQFIDKALDLGAAG
CHHHHHHHHHHEEECCCCCCCCEECCHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCE
VLVHTDDADQHGKVLLGDNAERGVQIYFFQLNRQVSAIAAQRYPLAHQFAVTPSLGIIGI
EEEECCCCCCCCCEEECCCCCCCEEEEEEEECCHHHHHHHHCCCCHHEEEECCCCCEEEE
TGTNGKTSTSQLIAQLVTSLRRKAAVMGTLGNGLWGELVDSGNTTADAITLMRQLHEFEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHC
QGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYATAKQSLFRFSTL
CCCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GHGLLNLDDPVGASWLIELKDMAAQIWGFSIEGHQAAAFYTKNTQFDDQGVSTTLVWPEG
CCCCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCCEEEEEECCCCCCCCCCEEEEECCCC
ETQIHSPLLGAFNLSNLLAALSALYLQGFDMEALAEQVQYLTPVAGRMERFTTADNITLV
CCHHCCCHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VDYAHTPDAIEQALTALRRHCAGDLWCVFGCGGDRDKGKRPLMGRAAEQYADRIMVTSDN
EEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCEEEEECCC
ARSEDPAQIISDIIQGLVTPERALTQVDRITAIKDVVGLAKSGDLILLAGKGHETYQEAA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHC
GVRHNYDERALARQLAEQSK
CCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]