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The map label for this gene is mraW

Identifier: 120597213

GI number: 120597213

Start: 412468

End: 413409

Strand: Direct

Name: mraW

Synonym: Sputw3181_0382

Alternate gene names: 120597213

Gene position: 412468-413409 (Clockwise)

Preceding gene: 120597212

Following gene: 120597214

Centisome position: 8.76

GC content: 46.82

Gene sequence:

>942_bases
ATGAGTCAGGAATTTGCCCATTTATCCGTTCTGTTAGCAGAAACGGTTGGCGGTTTAAACATCAAAGACGATGGCATCTA
TATCGATGGCACGTTTGGCCGCGGTGGTCATTCAAGACAGATATTGCAGCGGCTCGGTGACAATGGTCGATTGATTGCAA
TTGATCGCGATCCCCAAGCGATTGAGGCGGCAAAACAATTTAGTGATGATCCGCGCTTTCAAATTGTGCATGGCGGTTTT
GGCCAATTAGCCGATTACGTCGAAGATCTTGGGCTTGTGGGCAAAATTGATGGCGTATTACTCGATTTAGGTGTGTCATC
ACCTCAACTTGACGATGCCGAGCGCGGTTTTAGCTTTTTGCGAGATGGCCCACTCGATATGCGGATGGACAACAGCCAAG
GCCAAACAGCCGCACAATGGTTGGCTCACGCTGAAATTGAAGATATGGCTTGGGTATTTAAAACCTATGGTGAGGAAAAA
AACGCCCGTCATATCGCCCGTTGTATTGCTGCTGACCGTGACAAAACGCCATTTTTACGCACTAAAGATTTGGCCGATTT
AATTGCGCGTATCACGAAGAATAAAGAACGCAATAAGCACCCAGCAACCCGAGTGTTTCAGGCGATACGTATCTATATCA
ACAGTGAATTAGACCAAATTGATCAGGCGCTTGAAGGGGCATTAGCTGTACTCGCTCCCCAAGGGCGATTATCCATCATT
AGCTTTCATTCCTTAGAAGATCGTATCGTGAAGCGTTTTATCCGTCGTCATAGCCAAGGTGAGAGTGTGCCGTACGGTTT
GCCGATCACTGAAGATCAAATTAATAAGTCGCGTAAATTACGCGCCATTGGTAAGGCGATTATGCCATCCGATGAGGAAA
TTGAACGCAATGCCAGGGCTCGCAGCTCAGTATTACGCATTGCCGAGCGTTTAGATTACTAA

Upstream 100 bases:

>100_bases
GAATGTCAGGAAACTATTCGAAGTGAGGAGCTTGCTAATAACGAGCGCCTGGCAGATTTTTCACTCTAACGAGTCGCGCT
AATTATATTAAGAAGAGAGT

Downstream 100 bases:

>100_bases
GGGGCGAACTGTGAGTAAGCCCTCATTAAATTTACCACGAGTGGTACTACATGATTTGTGGTCACACAAATGGATTTTGT
TACTGGCTTTACTCGTGCTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQILQRLGDNGRLIAIDRDPQAIEAAKQFSDDPRFQIVHGGF
GQLADYVEDLGLVGKIDGVLLDLGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGQTAAQWLAHAEIEDMAWVFKTYGEEK
NARHIARCIAADRDKTPFLRTKDLADLIARITKNKERNKHPATRVFQAIRIYINSELDQIDQALEGALAVLAPQGRLSII
SFHSLEDRIVKRFIRRHSQGESVPYGLPITEDQINKSRKLRAIGKAIMPSDEEIERNARARSSVLRIAERLDY

Sequences:

>Translated_313_residues
MSQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQILQRLGDNGRLIAIDRDPQAIEAAKQFSDDPRFQIVHGGF
GQLADYVEDLGLVGKIDGVLLDLGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGQTAAQWLAHAEIEDMAWVFKTYGEEK
NARHIARCIAADRDKTPFLRTKDLADLIARITKNKERNKHPATRVFQAIRIYINSELDQIDQALEGALAVLAPQGRLSII
SFHSLEDRIVKRFIRRHSQGESVPYGLPITEDQINKSRKLRAIGKAIMPSDEEIERNARARSSVLRIAERLDY
>Mature_312_residues
SQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQILQRLGDNGRLIAIDRDPQAIEAAKQFSDDPRFQIVHGGFG
QLADYVEDLGLVGKIDGVLLDLGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGQTAAQWLAHAEIEDMAWVFKTYGEEKN
ARHIARCIAADRDKTPFLRTKDLADLIARITKNKERNKHPATRVFQAIRIYINSELDQIDQALEGALAVLAPQGRLSIIS
FHSLEDRIVKRFIRRHSQGESVPYGLPITEDQINKSRKLRAIGKAIMPSDEEIERNARARSSVLRIAERLDY

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=39.1176470588235, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=41.2371134020619, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=64.6496815286624, Blast_Score=413, Evalue=1e-117,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=39.0243902439024, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=39.0243902439024, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35056; Mature: 34925

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQILQRLGDNGRLIAIDRDPQA
CCCHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IEAAKQFSDDPRFQIVHGGFGQLADYVEDLGLVGKIDGVLLDLGVSSPQLDDAERGFSFL
HHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGQTAAQWLAHAEIEDMAWVFKTYGEEKNARHIARCIAADRDKTPFLR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
TKDLADLIARITKNKERNKHPATRVFQAIRIYINSELDQIDQALEGALAVLAPQGRLSII
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCEEEE
SFHSLEDRIVKRFIRRHSQGESVPYGLPITEDQINKSRKLRAIGKAIMPSDEEIERNARA
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RSSVLRIAERLDY
HHHHHHHHHHHCC
>Mature Secondary Structure 
SQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQILQRLGDNGRLIAIDRDPQA
CCHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IEAAKQFSDDPRFQIVHGGFGQLADYVEDLGLVGKIDGVLLDLGVSSPQLDDAERGFSFL
HHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGQTAAQWLAHAEIEDMAWVFKTYGEEKNARHIARCIAADRDKTPFLR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
TKDLADLIARITKNKERNKHPATRVFQAIRIYINSELDQIDQALEGALAVLAPQGRLSII
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCEEEE
SFHSLEDRIVKRFIRRHSQGESVPYGLPITEDQINKSRKLRAIGKAIMPSDEEIERNARA
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RSSVLRIAERLDY
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA