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The map label for this gene is azoR [H]

Identifier: 120597108

GI number: 120597108

Start: 302970

End: 303566

Strand: Direct

Name: azoR [H]

Synonym: Sputw3181_0277

Alternate gene names: 120597108

Gene position: 302970-303566 (Clockwise)

Preceding gene: 120597107

Following gene: 120597110

Centisome position: 6.43

GC content: 45.56

Gene sequence:

>597_bases
ATGTCTAAAGTCTTAATTTTAAAATCAAGCATTCTTGGCGGTTATTCACAATCTGCCGTGTTAATTGACCACTTAGCGAG
CCACTGGGAAAACCAAGGCGCCGCTATTACAGTACGTGATTTAGGCGGTAAAGACGTACTGCCTATGGTAGACGGTGAAA
TCGCTTCTGGGTTACGCGGTGGTGCAGAATTATCGGCACGTCAGCAAGAAATGCTCGCCTTATCAGATACCTTAGTCGCT
GAATTAAAAGCGAACGACACTATCGTTATCGCGGCACCTATGTATAACTTTACCATTCCAGCCCAACTTAAAAACTGGAT
CGATTTTATTGCCCGCGCCGGTGTGACTTTTACTTACACTGAAACCGGTTCAAAAGGATTAGTGGAAGGTAAACGCGCCG
TGTTGGTGACGACTCGTGGCGGCGCCCACAAAGATGGCCCAACCGATCACGTTGTACCTTACTTGAAAACGGTTTTAGCT
TTTATAGGTATCACCAATGTTGAAGTGGTTTACGCCGAAGCACTGAATATGGGGCCAGAAGCACACGATAAAGGCATGAG
CGAAGCCAAACACAGTATTGATTTGTTAACTGCTTAA

Upstream 100 bases:

>100_bases
TTATCCGCTTTATTTCTTTTTTATGAGCTAATACACTAGCGTCATAGAGTTGAGATATTCCATCTGACTCTGAGTCATTT
ACCTATTTTCGGAGCAGATT

Downstream 100 bases:

>100_bases
TAGTCAAATAAATTTAATAAAATGCCAGCTTATATAAGCTGCATTTTTGTTTGAGCAGTGCCAATAACCTATCTCACTTC
GGTTGCACCCTTGGTTTACC

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSKVLILKSSILGGYSQSAVLIDHLASHWENQGAAITVRDLGGKDVLPMVDGEIASGLRGGAELSARQQEMLALSDTLVA
ELKANDTIVIAAPMYNFTIPAQLKNWIDFIARAGVTFTYTETGSKGLVEGKRAVLVTTRGGAHKDGPTDHVVPYLKTVLA
FIGITNVEVVYAEALNMGPEAHDKGMSEAKHSIDLLTA

Sequences:

>Translated_198_residues
MSKVLILKSSILGGYSQSAVLIDHLASHWENQGAAITVRDLGGKDVLPMVDGEIASGLRGGAELSARQQEMLALSDTLVA
ELKANDTIVIAAPMYNFTIPAQLKNWIDFIARAGVTFTYTETGSKGLVEGKRAVLVTTRGGAHKDGPTDHVVPYLKTVLA
FIGITNVEVVYAEALNMGPEAHDKGMSEAKHSIDLLTA
>Mature_197_residues
SKVLILKSSILGGYSQSAVLIDHLASHWENQGAAITVRDLGGKDVLPMVDGEIASGLRGGAELSARQQEMLALSDTLVAE
LKANDTIVIAAPMYNFTIPAQLKNWIDFIARAGVTFTYTETGSKGLVEGKRAVLVTTRGGAHKDGPTDHVVPYLKTVLAF
IGITNVEVVYAEALNMGPEAHDKGMSEAKHSIDLLTA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=197, Percent_Identity=59.8984771573604, Blast_Score=233, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21058; Mature: 20927

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVLILKSSILGGYSQSAVLIDHLASHWENQGAAITVRDLGGKDVLPMVDGEIASGLRG
CCEEEEEEHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCC
GAELSARQQEMLALSDTLVAELKANDTIVIAAPMYNFTIPAQLKNWIDFIARAGVTFTYT
CCHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCEEEEE
ETGSKGLVEGKRAVLVTTRGGAHKDGPTDHVVPYLKTVLAFIGITNVEVVYAEALNMGPE
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCC
AHDKGMSEAKHSIDLLTA
HHHCCHHHHHHHEEEECC
>Mature Secondary Structure 
SKVLILKSSILGGYSQSAVLIDHLASHWENQGAAITVRDLGGKDVLPMVDGEIASGLRG
CEEEEEEHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCC
GAELSARQQEMLALSDTLVAELKANDTIVIAAPMYNFTIPAQLKNWIDFIARAGVTFTYT
CCHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCEEEEE
ETGSKGLVEGKRAVLVTTRGGAHKDGPTDHVVPYLKTVLAFIGITNVEVVYAEALNMGPE
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCC
AHDKGMSEAKHSIDLLTA
HHHCCHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA