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The map label for this gene is moaE [H]

Identifier: 120597077

GI number: 120597077

Start: 270393

End: 270854

Strand: Direct

Name: moaE [H]

Synonym: Sputw3181_0246

Alternate gene names: 120597077

Gene position: 270393-270854 (Clockwise)

Preceding gene: 120597076

Following gene: 120597078

Centisome position: 5.74

GC content: 49.57

Gene sequence:

>462_bases
ATGCGTTTACTACCCGCAGTTCGAGTGCAAGAGGTCGATTTTAGTGTTTCTGACGAATATCGCCAACTCGCCCAAGATGA
TAGCGATGGTGCCGTAGTCACTTTCGTCGGTAAAGTGCGTGATTTTAACGATGGCGCCGCGGTGACTGACTTAACCTTAG
AGCATTACCCTGGCATGACAGAAGCTGTACTCGAACAAATTGTTGCCGAAGCGCGTAGCCGTTGGCCGTTGAATCGGGTC
ACTGTTATCCACCGTGTCGGTACTATGTCGCTGGGCGAGCAAATTGTATTTATCGGCGTGACGAGTGCCCATCGTAAGGC
CGCTTTTGCTGCCTGTGAATTCTTAATCGACTTTTTAAAAACCAAAGCACCCTTCTGGAAATTAGAAGCGGGCGAGCAAG
GTAAAAGTTGGGTTGAGGCGAAAGATGCCGATGAGCAGGCGGCTAAATTGTGGCAAGCATAA

Upstream 100 bases:

>100_bases
AAATTGCTGGTGGCGGTAAATCAGACCATTAGCCAATGGGATACACCGGTAAAGGATGGTGATGAAGTCGCCTTTTTCCC
ACCAGTTACAGGAGGTTAAT

Downstream 100 bases:

>100_bases
AAGCTGGCGACAAACAGGCGTATATTGCTAACACAAATTAATGGAAGATTGACAACATGGTGGGTTTAACTCGTTTTTTT
AACTCAGTTTTTTTATCTGT

Product: molybdopterin biosynthesis MoaE

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MRLLPAVRVQEVDFSVSDEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMTEAVLEQIVAEARSRWPLNRV
TVIHRVGTMSLGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGEQGKSWVEAKDADEQAAKLWQA

Sequences:

>Translated_153_residues
MRLLPAVRVQEVDFSVSDEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMTEAVLEQIVAEARSRWPLNRV
TVIHRVGTMSLGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGEQGKSWVEAKDADEQAAKLWQA
>Mature_153_residues
MRLLPAVRVQEVDFSVSDEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMTEAVLEQIVAEARSRWPLNRV
TVIHRVGTMSLGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGEQGKSWVEAKDADEQAAKLWQA

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=120, Percent_Identity=37.5, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1787003, Length=138, Percent_Identity=63.768115942029, Blast_Score=189, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17509279, Length=125, Percent_Identity=36.8, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356259, Length=125, Percent_Identity=36.8, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 17025; Mature: 17025

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLPAVRVQEVDFSVSDEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMT
CCCCCCCEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHH
EAVLEQIVAEARSRWPLNRVTVIHRVGTMSLGEQIVFIGVTSAHRKAAFAACEFLIDFLK
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
TKAPFWKLEAGEQGKSWVEAKDADEQAAKLWQA
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MRLLPAVRVQEVDFSVSDEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMT
CCCCCCCEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHH
EAVLEQIVAEARSRWPLNRVTVIHRVGTMSLGEQIVFIGVTSAHRKAAFAACEFLIDFLK
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
TKAPFWKLEAGEQGKSWVEAKDADEQAAKLWQA
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]