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The map label for this gene is waaA [H]

Identifier: 120596885

GI number: 120596885

Start: 65662

End: 66927

Strand: Direct

Name: waaA [H]

Synonym: Sputw3181_0052

Alternate gene names: 120596885

Gene position: 65662-66927 (Clockwise)

Preceding gene: 120596879

Following gene: 120596887

Centisome position: 1.39

GC content: 49.29

Gene sequence:

>1266_bases
ATGAATCGTTTTCTCTATTCCACGATCTTATATTTATTATCCCCTTTGCTAATCGTCTATTTAGCATTTCGTGCTATTAA
AAGCCCTGATTACCGCGGTCGTTGGGGGGAGCGTTTTGGCTTAACAACACTCAAATCGACGGATTTATTGATCCACTCAG
TGTCTATGGGGGAAACACTCGCAGCCATTCCATTAATTCGCTTGATCCAGCAATCACACCCAGAACTGAGTATTACCGTC
ACAACAACCAGCCCAACGGGATCCGCCGAAGTGCGCAAAGCCTTTGGTGACTCAGTGCAGCATTGCTATTTACCCTTTGA
CTTACCTTGGTGTGTGCAGCGTTTCTTAAGCCAAGTCTCGCCCAAGTGGTGCGTGATTATGGAGACTGAGCTGTGGCCTA
ACTTAGTCGCCATTGTGGCAAAACGAGGTGTACGCTTGATGTTGGCTAATGCTCGCCTATCGGCAAAGTCGGCGGCGCAA
TATACTAAGCGCCCTAAACTCAGTCGACCTATGTTGCAACGACTCGATGTCATTGCGGCGCAGACTCAAGTGGAAGCGCA
GCGTTTTATCGAATTAGGTGTCTCGCCAGAGAGGGTGACTGTATGCGGCAGCTTGAAGTTTGATCTCAGTATTACGCCTG
AGCGTTTAGCCAATGCTAAGTTGTTACGCCAGACTTGGGGGCGAGAAACCTCGCCAATTTGGGTTGCGGGCAGTGTGCAC
CCAGGTGAATTTGACGCCATACTCGCGGCCCATAGGCAATTATTAGCTCGATGGCCCGATGCTTTGCTGATCATAGCTCC
GCGCCATCCGGAAAAGTTTACTGCCGTTGTCGAGGTTGTTGCGAGTCAAGGTTTTGAATTTGTTCGGCGCAGTGATGATC
AGCCCGTTACAGTAACGACTCAAGTGCTAGTGGGCGATACTATGGGCGAACTCTTAACTTTTTATGGCGCGGGGGATCAA
GCCTTTGTGGGCGGGAGTTTGATTCACCATGGTGGTCACAATCCATTGGAACCTATTGCTATGGGTGTACCTGTTATGGT
TGGGCCAAATTACCGAGATTTTGCCCAAATTACCCAAATGTTGGCCGATGCTGGAGGGCTAAGAATCGTGGTCTCGGCAG
ATGAGTTAGGGGTTAACTTGATCGAATATTTTGCAGAGCCAGAGCTACGTCAACAGGCTGCTAACGCGGGATTAGCTGTG
GTTGAGGCAAATCGCGGTGCACTTGAGCGGCAATTCACACTGGTACAATCCTTATTAAGCCATTAA

Upstream 100 bases:

>100_bases
CTAACCAACGACCGCCGCACTGCTGGTAAATTATCCCTAATTTGGTGCATATGCTAACATTGCCGCTATTCATCAGGCAA
AACCCATAAGCACGTTTTTC

Downstream 100 bases:

>100_bases
TTCTGTTAATTAAGCGCTATTGAGCAGATATATACCTAAAGCTGGCGAATTTAAGCGCTTTGCATTGCAGCTTGGTAGCC
TTGCATTAGTGAGGTCCAGT

Product: 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNRFLYSTILYLLSPLLIVYLAFRAIKSPDYRGRWGERFGLTTLKSTDLLIHSVSMGETLAAIPLIRLIQQSHPELSITV
TTTSPTGSAEVRKAFGDSVQHCYLPFDLPWCVQRFLSQVSPKWCVIMETELWPNLVAIVAKRGVRLMLANARLSAKSAAQ
YTKRPKLSRPMLQRLDVIAAQTQVEAQRFIELGVSPERVTVCGSLKFDLSITPERLANAKLLRQTWGRETSPIWVAGSVH
PGEFDAILAAHRQLLARWPDALLIIAPRHPEKFTAVVEVVASQGFEFVRRSDDQPVTVTTQVLVGDTMGELLTFYGAGDQ
AFVGGSLIHHGGHNPLEPIAMGVPVMVGPNYRDFAQITQMLADAGGLRIVVSADELGVNLIEYFAEPELRQQAANAGLAV
VEANRGALERQFTLVQSLLSH

Sequences:

>Translated_421_residues
MNRFLYSTILYLLSPLLIVYLAFRAIKSPDYRGRWGERFGLTTLKSTDLLIHSVSMGETLAAIPLIRLIQQSHPELSITV
TTTSPTGSAEVRKAFGDSVQHCYLPFDLPWCVQRFLSQVSPKWCVIMETELWPNLVAIVAKRGVRLMLANARLSAKSAAQ
YTKRPKLSRPMLQRLDVIAAQTQVEAQRFIELGVSPERVTVCGSLKFDLSITPERLANAKLLRQTWGRETSPIWVAGSVH
PGEFDAILAAHRQLLARWPDALLIIAPRHPEKFTAVVEVVASQGFEFVRRSDDQPVTVTTQVLVGDTMGELLTFYGAGDQ
AFVGGSLIHHGGHNPLEPIAMGVPVMVGPNYRDFAQITQMLADAGGLRIVVSADELGVNLIEYFAEPELRQQAANAGLAV
VEANRGALERQFTLVQSLLSH
>Mature_421_residues
MNRFLYSTILYLLSPLLIVYLAFRAIKSPDYRGRWGERFGLTTLKSTDLLIHSVSMGETLAAIPLIRLIQQSHPELSITV
TTTSPTGSAEVRKAFGDSVQHCYLPFDLPWCVQRFLSQVSPKWCVIMETELWPNLVAIVAKRGVRLMLANARLSAKSAAQ
YTKRPKLSRPMLQRLDVIAAQTQVEAQRFIELGVSPERVTVCGSLKFDLSITPERLANAKLLRQTWGRETSPIWVAGSVH
PGEFDAILAAHRQLLARWPDALLIIAPRHPEKFTAVVEVVASQGFEFVRRSDDQPVTVTTQVLVGDTMGELLTFYGAGDQ
AFVGGSLIHHGGHNPLEPIAMGVPVMVGPNYRDFAQITQMLADAGGLRIVVSADELGVNLIEYFAEPELRQQAANAGLAV
VEANRGALERQFTLVQSLLSH

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=421, Percent_Identity=47.0308788598575, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46501; Mature: 46501

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFLYSTILYLLSPLLIVYLAFRAIKSPDYRGRWGERFGLTTLKSTDLLIHSVSMGETL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCHH
AAIPLIRLIQQSHPELSITVTTTSPTGSAEVRKAFGDSVQHCYLPFDLPWCVQRFLSQVS
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCC
PKWCVIMETELWPNLVAIVAKRGVRLMLANARLSAKSAAQYTKRPKLSRPMLQRLDVIAA
CCEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
QTQVEAQRFIELGVSPERVTVCGSLKFDLSITPERLANAKLLRQTWGRETSPIWVAGSVH
HHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCC
PGEFDAILAAHRQLLARWPDALLIIAPRHPEKFTAVVEVVASQGFEFVRRSDDQPVTVTT
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEEEE
QVLVGDTMGELLTFYGAGDQAFVGGSLIHHGGHNPLEPIAMGVPVMVGPNYRDFAQITQM
EEEECCHHHHHHHHHCCCCCEEECCHHHCCCCCCCCCHHHCCCCEEECCCHHHHHHHHHH
LADAGGLRIVVSADELGVNLIEYFAEPELRQQAANAGLAVVEANRGALERQFTLVQSLLS
HHCCCCEEEEEECHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHC
H
C
>Mature Secondary Structure
MNRFLYSTILYLLSPLLIVYLAFRAIKSPDYRGRWGERFGLTTLKSTDLLIHSVSMGETL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCHH
AAIPLIRLIQQSHPELSITVTTTSPTGSAEVRKAFGDSVQHCYLPFDLPWCVQRFLSQVS
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCC
PKWCVIMETELWPNLVAIVAKRGVRLMLANARLSAKSAAQYTKRPKLSRPMLQRLDVIAA
CCEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
QTQVEAQRFIELGVSPERVTVCGSLKFDLSITPERLANAKLLRQTWGRETSPIWVAGSVH
HHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCC
PGEFDAILAAHRQLLARWPDALLIIAPRHPEKFTAVVEVVASQGFEFVRRSDDQPVTVTT
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEEEE
QVLVGDTMGELLTFYGAGDQAFVGGSLIHHGGHNPLEPIAMGVPVMVGPNYRDFAQITQM
EEEECCHHHHHHHHHCCCCCEEECCHHHCCCCCCCCCHHHCCCCEEECCCHHHHHHHHHH
LADAGGLRIVVSADELGVNLIEYFAEPELRQQAANAGLAVVEANRGALERQFTLVQSLLS
HHCCCCEEEEEECHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]