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The map label for this gene is fruB [H]

Identifier: 119947153

GI number: 119947153

Start: 4368602

End: 4369723

Strand: Direct

Name: fruB [H]

Synonym: Ping_3551

Alternate gene names: 119947153

Gene position: 4368602-4369723 (Clockwise)

Preceding gene: 119947151

Following gene: 119947154

Centisome position: 95.81

GC content: 42.07

Gene sequence:

>1122_bases
ATGTTATCAATTAATACCCACGATATTATTCTTTCCCAAGCCGCAGAGAATAAACAAGATGCAATTAAATCTATCGCGAA
CGGCCTGATGGATAAGGGTTATGTCGAAATCGGTTATGTTAACGGTATGCTCGCTCGTGAAGCCCAAAACACCACTTTTT
TAGGTAATGGTATCGCCATCCCTCATGGCACAACGGATACCCGTGATCAGATAAAACAAACCGGTGTGATTTTGCATCAC
TTTCCTCAAGGTGTGAATTGGGGTGATGATAATATGGTTTATTTAGCAATAGGTATCGCAGCAAAATCAGATCAGCATTT
AGAAATTTTAAAAATGCTGACGAAAGTACTTTCTGCTGAGGGTGTTGAACAACAGTTACAAAATGCAACCACTGCTGAAA
CCATTGTTGATTTATTAACCGGCAAAGTGCAAACCGAAATGTTGTTAACGACTGACTTGATTCAACTCGAGACTGCAGCA
CAGGATATCTCTCAACTAACAGCGCTTGCAGCCGATTTAATCAAAAATCAGGGAGCGGTCGGTGATACTTTTGCTGATGC
AATAGCGGCTAACAATCCAACGCATTTAGGCCAAGGTCTGTGGTTAACTGCCAGCAACCAAGGTGTTAATAAAACTGCCC
TCTCTTTTGTAAAAGTATCCCAATCATTCCAGCATTTAGATAAACCAGTCAGCGGCCTTCTCTGTATCGCCAGTAACAGT
GAACTGCATTTAAAAACGCTGGATTTTCTGATTGATATGATTGCCAAACAAAAAGTAGATAAGTTCTTCAGTTCGAATCA
AGAACAAGTTATCAACTTACTGACAAAAGAAACGCTCGAAGGTACTCAAAAAACATTCACTATCCGTAATCCGCATGGTT
TACATGCCCGCCCGGGTGCGATGCTGGTTAATACGGCGAAAAAATTTGCAGCGACCATTCAGCTGGCAAAAACAGATACG
CCGGACAAGACAGTCAATGCAAAAAGTTTAATGAAAGTGATGACCTTGGGGGTCAAGTTTAAAGACCAGTTACAGGTTAC
CGCACAGGGTGAAGATGCCGATCAGGCACTGCTTGCCATTGGTCAAGCGATTGAGGACGGCCTTGGAGAAAAAATCCAAT
GA

Upstream 100 bases:

>100_bases
ATTGGCTCACAATTATACTGACGACTAAGCGCTCATTTTTAAACCATTTGTGTCTGCGTTAATAAGCGCGACAGTCACTA
ACATTAGTAAGGTACCCTGC

Downstream 100 bases:

>100_bases
GTAAAAATCTAAAAGTGGTTACTGTCACCTTAAACCCGGCCTTAGATCTTACCGGCTCACTTGAGGCATTACAGACAGGG
TCTGTGAATTTAATTGAAAC

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLSINTHDIILSQAAENKQDAIKSIANGLMDKGYVEIGYVNGMLAREAQNTTFLGNGIAIPHGTTDTRDQIKQTGVILHH
FPQGVNWGDDNMVYLAIGIAAKSDQHLEILKMLTKVLSAEGVEQQLQNATTAETIVDLLTGKVQTEMLLTTDLIQLETAA
QDISQLTALAADLIKNQGAVGDTFADAIAANNPTHLGQGLWLTASNQGVNKTALSFVKVSQSFQHLDKPVSGLLCIASNS
ELHLKTLDFLIDMIAKQKVDKFFSSNQEQVINLLTKETLEGTQKTFTIRNPHGLHARPGAMLVNTAKKFAATIQLAKTDT
PDKTVNAKSLMKVMTLGVKFKDQLQVTAQGEDADQALLAIGQAIEDGLGEKIQ

Sequences:

>Translated_373_residues
MLSINTHDIILSQAAENKQDAIKSIANGLMDKGYVEIGYVNGMLAREAQNTTFLGNGIAIPHGTTDTRDQIKQTGVILHH
FPQGVNWGDDNMVYLAIGIAAKSDQHLEILKMLTKVLSAEGVEQQLQNATTAETIVDLLTGKVQTEMLLTTDLIQLETAA
QDISQLTALAADLIKNQGAVGDTFADAIAANNPTHLGQGLWLTASNQGVNKTALSFVKVSQSFQHLDKPVSGLLCIASNS
ELHLKTLDFLIDMIAKQKVDKFFSSNQEQVINLLTKETLEGTQKTFTIRNPHGLHARPGAMLVNTAKKFAATIQLAKTDT
PDKTVNAKSLMKVMTLGVKFKDQLQVTAQGEDADQALLAIGQAIEDGLGEKIQ
>Mature_373_residues
MLSINTHDIILSQAAENKQDAIKSIANGLMDKGYVEIGYVNGMLAREAQNTTFLGNGIAIPHGTTDTRDQIKQTGVILHH
FPQGVNWGDDNMVYLAIGIAAKSDQHLEILKMLTKVLSAEGVEQQLQNATTAETIVDLLTGKVQTEMLLTTDLIQLETAA
QDISQLTALAADLIKNQGAVGDTFADAIAANNPTHLGQGLWLTASNQGVNKTALSFVKVSQSFQHLDKPVSGLLCIASNS
ELHLKTLDFLIDMIAKQKVDKFFSSNQEQVINLLTKETLEGTQKTFTIRNPHGLHARPGAMLVNTAKKFAATIQLAKTDT
PDKTVNAKSLMKVMTLGVKFKDQLQVTAQGEDADQALLAIGQAIEDGLGEKIQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=374, Percent_Identity=45.1871657754011, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1790027, Length=144, Percent_Identity=36.1111111111111, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 40265; Mature: 40265

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSINTHDIILSQAAENKQDAIKSIANGLMDKGYVEIGYVNGMLAREAQNTTFLGNGIAI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCEEECCCEEC
PHGTTDTRDQIKQTGVILHHFPQGVNWGDDNMVYLAIGIAAKSDQHLEILKMLTKVLSAE
CCCCCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCC
GVEQQLQNATTAETIVDLLTGKVQTEMLLTTDLIQLETAAQDISQLTALAADLIKNQGAV
CHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDTFADAIAANNPTHLGQGLWLTASNQGVNKTALSFVKVSQSFQHLDKPVSGLLCIASNS
CHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
ELHLKTLDFLIDMIAKQKVDKFFSSNQEQVINLLTKETLEGTQKTFTIRNPHGLHARPGA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCH
MLVNTAKKFAATIQLAKTDTPDKTVNAKSLMKVMTLGVKFKDQLQVTAQGEDADQALLAI
HHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
GQAIEDGLGEKIQ
HHHHHHCCCCCCC
>Mature Secondary Structure
MLSINTHDIILSQAAENKQDAIKSIANGLMDKGYVEIGYVNGMLAREAQNTTFLGNGIAI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCEEECCCEEC
PHGTTDTRDQIKQTGVILHHFPQGVNWGDDNMVYLAIGIAAKSDQHLEILKMLTKVLSAE
CCCCCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCC
GVEQQLQNATTAETIVDLLTGKVQTEMLLTTDLIQLETAAQDISQLTALAADLIKNQGAV
CHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDTFADAIAANNPTHLGQGLWLTASNQGVNKTALSFVKVSQSFQHLDKPVSGLLCIASNS
CHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
ELHLKTLDFLIDMIAKQKVDKFFSSNQEQVINLLTKETLEGTQKTFTIRNPHGLHARPGA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCH
MLVNTAKKFAATIQLAKTDTPDKTVNAKSLMKVMTLGVKFKDQLQVTAQGEDADQALLAI
HHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
GQAIEDGLGEKIQ
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]