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The map label for this gene is def2 [H]

Identifier: 119946929

GI number: 119946929

Start: 4105586

End: 4106134

Strand: Direct

Name: def2 [H]

Synonym: Ping_3323

Alternate gene names: 119946929

Gene position: 4105586-4106134 (Clockwise)

Preceding gene: 119946928

Following gene: 119946933

Centisome position: 90.04

GC content: 40.62

Gene sequence:

>549_bases
ATGAATCAAACTAAAAACAAGTCATATCCCATTGCTCAATTGGGCCATCCAGTATTAAGACAAAGAGCGACTGAGGTTGA
AAATGTGCTTGCCGATGAATGTCAGCAATTAATTAATCAAATGATGTTCGCTGTATCAGAAGCCGGTGGTGTCGGTATTG
CAGCACCACAAATTCATCATAGTGTGCGCATGTTTATTATGTGTTCTAAACCCAATGCTCGTTACCCGGACGCGCCATTA
ATGGCACCAACAGCGATCATTAACCCTGAAATATTGCATTATTCCAGTGACAAAGTGAAAGGCTGGGAAGGCTGTTTAAG
TGTACCTAGTATGCGCGGTTTAGTACCAAGGCATAGCCAAATCACTGTCCGTTATGTTGATCAACAAGGTAATAAACAGC
AGCAGGAATTAACGGGGTTTATTGCCCGTATTTTTCAGCACGAATTAGATCATTTGAACGGCTTAACCTTTATTGACCAG
TTAGAATCGACGCAAGATTTAATCAGTGAAGTTGAATGGTATCAACAGTTCGCTTCTATACAAAGTTAA

Upstream 100 bases:

>100_bases
CGGGTTGCCACCTGTTTTAGGATCTATCTACGTTCAAAAATGGTTTGCGGGCGAGAGTAAAAATATTGAAGTAACGATTA
AGAGAAAAGACTAAAGCGAA

Downstream 100 bases:

>100_bases
CTGTTTTCAGGCTCCACTCACTCGATAATTCATTTTTAAGCGGGCAGAGTAGAATCAATTAATTGCTTCTTCAACTGTTT
TTTTTCTAAACGGCGGCGCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNQTKNKSYPIAQLGHPVLRQRATEVENVLADECQQLINQMMFAVSEAGGVGIAAPQIHHSVRMFIMCSKPNARYPDAPL
MAPTAIINPEILHYSSDKVKGWEGCLSVPSMRGLVPRHSQITVRYVDQQGNKQQQELTGFIARIFQHELDHLNGLTFIDQ
LESTQDLISEVEWYQQFASIQS

Sequences:

>Translated_182_residues
MNQTKNKSYPIAQLGHPVLRQRATEVENVLADECQQLINQMMFAVSEAGGVGIAAPQIHHSVRMFIMCSKPNARYPDAPL
MAPTAIINPEILHYSSDKVKGWEGCLSVPSMRGLVPRHSQITVRYVDQQGNKQQQELTGFIARIFQHELDHLNGLTFIDQ
LESTQDLISEVEWYQQFASIQS
>Mature_182_residues
MNQTKNKSYPIAQLGHPVLRQRATEVENVLADECQQLINQMMFAVSEAGGVGIAAPQIHHSVRMFIMCSKPNARYPDAPL
MAPTAIINPEILHYSSDKVKGWEGCLSVPSMRGLVPRHSQITVRYVDQQGNKQQQELTGFIARIFQHELDHLNGLTFIDQ
LESTQDLISEVEWYQQFASIQS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=165, Percent_Identity=31.5151515151515, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1789682, Length=143, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24645728, Length=162, Percent_Identity=36.4197530864198, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645726, Length=165, Percent_Identity=35.1515151515151, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20533; Mature: 20533

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTKNKSYPIAQLGHPVLRQRATEVENVLADECQQLINQMMFAVSEAGGVGIAAPQIHH
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SVRMFIMCSKPNARYPDAPLMAPTAIINPEILHYSSDKVKGWEGCLSVPSMRGLVPRHSQ
CEEEEEEECCCCCCCCCCCCCCCHHHCCCHHEECCCCCCCCHHHHHCCCHHHCCCCCCCE
ITVRYVDQQGNKQQQELTGFIARIFQHELDHLNGLTFIDQLESTQDLISEVEWYQQFASI
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QS
CC
>Mature Secondary Structure
MNQTKNKSYPIAQLGHPVLRQRATEVENVLADECQQLINQMMFAVSEAGGVGIAAPQIHH
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SVRMFIMCSKPNARYPDAPLMAPTAIINPEILHYSSDKVKGWEGCLSVPSMRGLVPRHSQ
CEEEEEEECCCCCCCCCCCCCCCHHHCCCHHEECCCCCCCCHHHHHCCCHHHCCCCCCCE
ITVRYVDQQGNKQQQELTGFIARIFQHELDHLNGLTFIDQLESTQDLISEVEWYQQFASI
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]