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The map label for this gene is glpF [H]

Identifier: 119946773

GI number: 119946773

Start: 3897806

End: 3898384

Strand: Direct

Name: glpF [H]

Synonym: Ping_3166

Alternate gene names: 119946773

Gene position: 3897806-3898384 (Clockwise)

Preceding gene: 119946772

Following gene: 119946774

Centisome position: 85.49

GC content: 43.18

Gene sequence:

>579_bases
ATGCATCGCGAGCTTATAGGCACGGGCCTGCTGATATTTTTTGTTGTCGGTTGTATTGCCACGCTAGTGTTAACAGGGGT
AAGTTTTGGCCAATGGAAAATCAGTATCATGTTGGATATGAGCGGTGCCATATGCTGTACTGCCGGTATTTTTGGTGCGC
ATATAAATCCTGCAGCGACCATCACGCTGGCTGCTTTTCTTGGTTTTGAAAAAGAAAAGTCTTACCTTTATATAGCTGCG
CAACTGGCAGCCACATTTTGTAGTGCAGCTTTAATTTATGCTTTCAGCAATCTGTTTACTGACGATGAAATAGCTCACGG
CTTTTTACACAATAGTGAGGCGGCATTATCAACTGCCGGTATTTTCTCAACCTACCCGCTCCCGTCATTCTTTATTCTTG
GCACATTTTCAATCAAATTTTTCATTACTGCTGTAATTATATTCGCTATTTTAGCGCTCAATAATCAAAGCAACGGTGCA
CCGCAGGGTGTAATGCCCTCTTTTAATCGGTCTTGTAGTTGCAGTTATCGGCCCTTCTTTAGACCCGCGAACGGGTTTTG
TGATAAATCTGGCGAGTGA

Upstream 100 bases:

>100_bases
ATGTTTCATAAATGTTAAAATAACGTACATTTGCGCAATATAGAATGCATTAACCTTAAATAAAGATCATATCCAAAGGC
AATATACACTGCAGGGGGTA

Downstream 100 bases:

>100_bases
TTTGGATCCTAAGTTATTTACTTACTTCACCAGCCGGGCTTATTCGAAAATGATTGCACAATATTTAGCAAACGTCGGTC
ACAGCAGCACAATTCCAAAC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MHRELIGTGLLIFFVVGCIATLVLTGVSFGQWKISIMLDMSGAICCTAGIFGAHINPAATITLAAFLGFEKEKSYLYIAA
QLAATFCSAALIYAFSNLFTDDEIAHGFLHNSEAALSTAGIFSTYPLPSFFILGTFSIKFFITAVIIFAILALNNQSNGA
PQGVMPSFNRSCSCSYRPFFRPANGFCDKSGE

Sequences:

>Translated_192_residues
MHRELIGTGLLIFFVVGCIATLVLTGVSFGQWKISIMLDMSGAICCTAGIFGAHINPAATITLAAFLGFEKEKSYLYIAA
QLAATFCSAALIYAFSNLFTDDEIAHGFLHNSEAALSTAGIFSTYPLPSFFILGTFSIKFFITAVIIFAILALNNQSNGA
PQGVMPSFNRSCSCSYRPFFRPANGFCDKSGE
>Mature_192_residues
MHRELIGTGLLIFFVVGCIATLVLTGVSFGQWKISIMLDMSGAICCTAGIFGAHINPAATITLAAFLGFEKEKSYLYIAA
QLAATFCSAALIYAFSNLFTDDEIAHGFLHNSEAALSTAGIFSTYPLPSFFILGTFSIKFFITAVIIFAILALNNQSNGA
PQGVMPSFNRSCSCSYRPFFRPANGFCDKSGE

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Escherichia coli, GI1790362, Length=163, Percent_Identity=47.239263803681, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 20606; Mature: 20606

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRELIGTGLLIFFVVGCIATLVLTGVSFGQWKISIMLDMSGAICCTAGIFGAHINPAAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEHHHHHHCCCCCCHHH
ITLAAFLGFEKEKSYLYIAAQLAATFCSAALIYAFSNLFTDDEIAHGFLHNSEAALSTAG
HHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCC
IFSTYPLPSFFILGTFSIKFFITAVIIFAILALNNQSNGAPQGVMPSFNRSCSCSYRPFF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPANGFCDKSGE
CCCCCCCCCCCC
>Mature Secondary Structure
MHRELIGTGLLIFFVVGCIATLVLTGVSFGQWKISIMLDMSGAICCTAGIFGAHINPAAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEHHHHHHCCCCCCHHH
ITLAAFLGFEKEKSYLYIAAQLAATFCSAALIYAFSNLFTDDEIAHGFLHNSEAALSTAG
HHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCC
IFSTYPLPSFFILGTFSIKFFITAVIIFAILALNNQSNGAPQGVMPSFNRSCSCSYRPFF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPANGFCDKSGE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]