| Definition | Psychromonas ingrahamii 37, complete genome. |
|---|---|
| Accession | NC_008709 |
| Length | 4,559,598 |
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The map label for this gene is cheR [C]
Identifier: 119946742
GI number: 119946742
Start: 3850842
End: 3853364
Strand: Direct
Name: cheR [C]
Synonym: Ping_3128
Alternate gene names: 119946742
Gene position: 3850842-3853364 (Clockwise)
Preceding gene: 119946740
Following gene: 119946743
Centisome position: 84.46
GC content: 44.71
Gene sequence:
>2523_bases GTGATTAATACCCCCGTTATTGTCGGCATAGGGGCTTCTGCTGGCGGCTTTGAAGCATTGGAAGAATTCTTCTCTCATGT GCTAACACCTTGTAATATCGCATTTGTTGTCATCCAGCATCTTGACCCCACTCACAAAGGGATGATGCCTGAGCTGTTGC AACGCGACACAACGATGAAAGTAAAACAAGCTCGCAACAATATGAAGGTAAAGCCCAACTGCGTGTATGTGATCCCACCT GACAAAGATTTATCAGTCCTTCACGGGGTATTATTTCTGTTAGAACCGGTGGTTAAGCACACGATTCGGTTACCCATTGA TTCATTCTTTCAATCTTTGGCCGCTGATCAGCATGAACGCGCTGTGGGTGTTATTTTGTCAGGCATGGGATCCGATGGCA CGATTGGTTTACGCGCCATTAAAGAAAATGCCGGACTGTCTCTAGTACAGTCGCCTGAGTCAGCTAAATTTGATTCAATG CCTCAAAGTGCTATTAATGCCGGGCTAGCTGACATCACAGCCCCTGCTGAAGAACTACCGGAAAGGATTATAGCGTTTCT CGAACATGGCCGACGAGGTGTTCCAAACAAACTTGAACCGATAGTGAAACGCAAGTCAATAAGCGCACTAGCGCAGATCA TGATGATACTGCGTGAACGCAATGGAAACGACTTCTCACTGTACAAGACAAATACCATAGATCGTCGTATTGAACGGCGT ATGGGTCTGCATCAGCTTAAAACCATCTCGCTCTATGCCCGTTATCTACGTGATAATCCGCAAGAACAAGACCTGTTATT CAAGGAGCTGTTAATTGGGGTAACCAATTTCTTTCGTGATAAAGACGTTTGGGCGCAGCTCAAGTCAACCTCACTGCCAG CACTACTGGCCAATTATCCTGAAGGTAAAGAACTGCGGGCATGGGTAACCGCTTGCTCATCTGGTGAAGAGGCTTACTCT CTCGCAATAACGGTAATGGATGTATTGGATGAAATTAAGCCCAAAGGGCATTTTACATTACAAATTTTTGCTACCGATCT TAATCAGGATGCCATCAATATTGCAAGGAAAGGCTATTATCCGGCAGGCATTGAGGCTGACATGTCCGCACAGCAATTGC AGCGTTATTTTATTAAAGATGGCAGTGGTTACCGCATAAACAAGAAAATTCGTGACATGGTGATCTTCGCACCACAGAAC ATCATTATGGATCCCCCTTTCACTCGATTAGATATTCTCACCTGTCGTAATCTTCTGATTTATTTTGCACCGGAATTACA AAAAAAGTTACTGCCACTGTTCCACTACACATTAACTTCTCATGGGCTTTTGATTCTGGGCCATTCCGAAACGATTGGTA ATGCCACCTCATTGTTCTCAGAAATCAAAGAAAACACCCGGATTTACACGCGCATTGACAGGCCTGGACAACAGATGGAA GTCAACTTTCCTACTAAGATATTTCCGATTATGCCCCTGGCAGAAAATGAACATGAGGGAACCCGTAAGATGACGAAAAA TATTAGCAATCTACAAACGCAGGCAGATCAAATTCTGTTGCAAAACTATTCGCCAGCAGCGGTGTTAGTCAATGCGGCAG GCGACATTATCTATATCAATGGTCGTACCGGAAAATATTTAGAGCCTGCTGCGGGCAAAGCCAACTGGAACATTCACGTA ATGGCACGTGAAGAACTACAACATCAGCTTGAGCTCGCTATCAAGAAAGCACAGATCCAGGTAGAGCCAGTGACGATTGA AAATATCACAGTAGATGGACACAGTGTCAATTTGACGGTACAAGCCATTATCAAGCCAAAAGAGCTGCTTGGCTTAATAA TGGTGGTGTTTACTGAAGTAGTGAGACCGACCAGGCGGCGACGCAAAAAACTGGGGGCAGTGGAGCAAGAAAGTCAGGCC GAACTACAAATGGCGCATGATGAAATAAAATCCCTCCGCGAACAAATGCAATCCTCTCATGAGGAACTAAAGTCCGCCAA TGAAGAGTTGCAATCAACGAATGAAGAGTTGCAATCCACCAATGAAGAATTGACGACCTCTAAAGAAGAGATGCAGTCAA TGAACGAAGAGTTGCAAACCGTCAACACCGAACTGCAATCCAACGTTGATGATTTGTCATGGGTCAACAATGACATGGAG AACCTGCTTAACAGTACAGAAATTGCCACCATTTTTCTGGACAAAGGACTGCATATACGGCGCTTCACCAACCATGCAAC TCACTTATTCAAGCTGATTGAAGGGGATGTGGGCCGACTATTGTCCGACATTGTTACGGATCTGGACTATAAAGATCTGC AAAAAGATGCGAAGGAGGTATTGAAGAGACTGGCCTTCGTAGAAAAAGAGATAACGGCTAACAATGAACGCTGCTTCAAA GTCCGGATCATGCCTTATCGTACCCAAGAGAATGTGATTGACGGCGTGGTGATAACCTTCACCAATATTAGTGAAGCCAA ACTGCTTGAGTCAGAATTACGCAAGACGAGGTGCAAAACATGA
Upstream 100 bases:
>100_bases ATTGTATCTGCAATTATTTGATAACTTATTAAGCAATATACCCTATGAAAAAACAAAGCAAAGCATTGCCTAAATCTCCT TGGATTCCTGTAGATTGGTA
Downstream 100 bases:
>100_bases AGACACCTGCAAAGTCAACCAAGCTATCCAGCAAAACGTTACGCCGGCGCGCGGAAGCACAACTGAAAACACAAACAGAA ATCAAACAAACTATTCCTAG
Product: MCP methyltransferase, CheR-type
Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]
Alternate protein names: ORF19 [H]
Number of amino acids: Translated: 840; Mature: 840
Protein sequence:
>840_residues MINTPVIVGIGASAGGFEALEEFFSHVLTPCNIAFVVIQHLDPTHKGMMPELLQRDTTMKVKQARNNMKVKPNCVYVIPP DKDLSVLHGVLFLLEPVVKHTIRLPIDSFFQSLAADQHERAVGVILSGMGSDGTIGLRAIKENAGLSLVQSPESAKFDSM PQSAINAGLADITAPAEELPERIIAFLEHGRRGVPNKLEPIVKRKSISALAQIMMILRERNGNDFSLYKTNTIDRRIERR MGLHQLKTISLYARYLRDNPQEQDLLFKELLIGVTNFFRDKDVWAQLKSTSLPALLANYPEGKELRAWVTACSSGEEAYS LAITVMDVLDEIKPKGHFTLQIFATDLNQDAINIARKGYYPAGIEADMSAQQLQRYFIKDGSGYRINKKIRDMVIFAPQN IIMDPPFTRLDILTCRNLLIYFAPELQKKLLPLFHYTLTSHGLLILGHSETIGNATSLFSEIKENTRIYTRIDRPGQQME VNFPTKIFPIMPLAENEHEGTRKMTKNISNLQTQADQILLQNYSPAAVLVNAAGDIIYINGRTGKYLEPAAGKANWNIHV MAREELQHQLELAIKKAQIQVEPVTIENITVDGHSVNLTVQAIIKPKELLGLIMVVFTEVVRPTRRRRKKLGAVEQESQA ELQMAHDEIKSLREQMQSSHEELKSANEELQSTNEELQSTNEELTTSKEEMQSMNEELQTVNTELQSNVDDLSWVNNDME NLLNSTEIATIFLDKGLHIRRFTNHATHLFKLIEGDVGRLLSDIVTDLDYKDLQKDAKEVLKRLAFVEKEITANNERCFK VRIMPYRTQENVIDGVVITFTNISEAKLLESELRKTRCKT
Sequences:
>Translated_840_residues MINTPVIVGIGASAGGFEALEEFFSHVLTPCNIAFVVIQHLDPTHKGMMPELLQRDTTMKVKQARNNMKVKPNCVYVIPP DKDLSVLHGVLFLLEPVVKHTIRLPIDSFFQSLAADQHERAVGVILSGMGSDGTIGLRAIKENAGLSLVQSPESAKFDSM PQSAINAGLADITAPAEELPERIIAFLEHGRRGVPNKLEPIVKRKSISALAQIMMILRERNGNDFSLYKTNTIDRRIERR MGLHQLKTISLYARYLRDNPQEQDLLFKELLIGVTNFFRDKDVWAQLKSTSLPALLANYPEGKELRAWVTACSSGEEAYS LAITVMDVLDEIKPKGHFTLQIFATDLNQDAINIARKGYYPAGIEADMSAQQLQRYFIKDGSGYRINKKIRDMVIFAPQN IIMDPPFTRLDILTCRNLLIYFAPELQKKLLPLFHYTLTSHGLLILGHSETIGNATSLFSEIKENTRIYTRIDRPGQQME VNFPTKIFPIMPLAENEHEGTRKMTKNISNLQTQADQILLQNYSPAAVLVNAAGDIIYINGRTGKYLEPAAGKANWNIHV MAREELQHQLELAIKKAQIQVEPVTIENITVDGHSVNLTVQAIIKPKELLGLIMVVFTEVVRPTRRRRKKLGAVEQESQA ELQMAHDEIKSLREQMQSSHEELKSANEELQSTNEELQSTNEELTTSKEEMQSMNEELQTVNTELQSNVDDLSWVNNDME NLLNSTEIATIFLDKGLHIRRFTNHATHLFKLIEGDVGRLLSDIVTDLDYKDLQKDAKEVLKRLAFVEKEITANNERCFK VRIMPYRTQENVIDGVVITFTNISEAKLLESELRKTRCKT >Mature_840_residues MINTPVIVGIGASAGGFEALEEFFSHVLTPCNIAFVVIQHLDPTHKGMMPELLQRDTTMKVKQARNNMKVKPNCVYVIPP DKDLSVLHGVLFLLEPVVKHTIRLPIDSFFQSLAADQHERAVGVILSGMGSDGTIGLRAIKENAGLSLVQSPESAKFDSM PQSAINAGLADITAPAEELPERIIAFLEHGRRGVPNKLEPIVKRKSISALAQIMMILRERNGNDFSLYKTNTIDRRIERR MGLHQLKTISLYARYLRDNPQEQDLLFKELLIGVTNFFRDKDVWAQLKSTSLPALLANYPEGKELRAWVTACSSGEEAYS LAITVMDVLDEIKPKGHFTLQIFATDLNQDAINIARKGYYPAGIEADMSAQQLQRYFIKDGSGYRINKKIRDMVIFAPQN IIMDPPFTRLDILTCRNLLIYFAPELQKKLLPLFHYTLTSHGLLILGHSETIGNATSLFSEIKENTRIYTRIDRPGQQME VNFPTKIFPIMPLAENEHEGTRKMTKNISNLQTQADQILLQNYSPAAVLVNAAGDIIYINGRTGKYLEPAAGKANWNIHV MAREELQHQLELAIKKAQIQVEPVTIENITVDGHSVNLTVQAIIKPKELLGLIMVVFTEVVRPTRRRRKKLGAVEQESQA ELQMAHDEIKSLREQMQSSHEELKSANEELQSTNEELQSTNEELTTSKEEMQSMNEELQTVNTELQSNVDDLSWVNNDME NLLNSTEIATIFLDKGLHIRRFTNHATHLFKLIEGDVGRLLSDIVTDLDYKDLQKDAKEVLKRLAFVEKEITANNERCFK VRIMPYRTQENVIDGVVITFTNISEAKLLESELRKTRCKT
Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]
COG id: COG1352
COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 cheR-type methyltransferase domain [H]
Homologues:
Organism=Escherichia coli, GI1788193, Length=263, Percent_Identity=29.6577946768061, Blast_Score=100, Evalue=3e-22, Organism=Escherichia coli, GI1788192, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=7e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022642 - InterPro: IPR000780 - InterPro: IPR022641 - InterPro: IPR000014 - InterPro: IPR013656 - InterPro: IPR000673 [H]
Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]
EC number: 2.1.1.80 [C]
Molecular weight: Translated: 94944; Mature: 94944
Theoretical pI: Translated: 6.56; Mature: 6.56
Prosite motif: PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB ; PS00639 THIOL_PROTEASE_HIS
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MINTPVIVGIGASAGGFEALEEFFSHVLTPCNIAFVVIQHLDPTHKGMMPELLQRDTTMK CCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHEECCCCCCCCCCHHHHCCCCHHH VKQARNNMKVKPNCVYVIPPDKDLSVLHGVLFLLEPVVKHTIRLPIDSFFQSLAADQHER HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHH AVGVILSGMGSDGTIGLRAIKENAGLSLVQSPESAKFDSMPQSAINAGLADITAPAEELP HHHHHEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHCCHHHCCCCHHHHH ERIIAFLEHGRRGVPNKLEPIVKRKSISALAQIMMILRERNGNDFSLYKTNTIDRRIERR HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHH MGLHQLKTISLYARYLRDNPQEQDLLFKELLIGVTNFFRDKDVWAQLKSTSLPALLANYP HCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCC EGKELRAWVTACSSGEEAYSLAITVMDVLDEIKPKGHFTLQIFATDLNQDAINIARKGYY CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHCCCC PAGIEADMSAQQLQRYFIKDGSGYRINKKIRDMVIFAPQNIIMDPPFTRLDILTCRNLLI CCCCCCCCCHHHHHHHHHCCCCCCEECHHHHCEEEECCCCEECCCCCCHHHHHHHHHHHH YFAPELQKKLLPLFHYTLTSHGLLILGHSETIGNATSLFSEIKENTRIYTRIDRPGQQME HCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEE VNFPTKIFPIMPLAENEHEGTRKMTKNISNLQTQADQILLQNYSPAAVLVNAAGDIIYIN ECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEE GRTGKYLEPAAGKANWNIHVMAREELQHQLELAIKKAQIQVEPVTIENITVDGHSVNLTV CCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCEEEEEE QAIIKPKELLGLIMVVFTEVVRPTRRRRKKLGAVEQESQAELQMAHDEIKSLREQMQSSH EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH EELKSANEELQSTNEELQSTNEELTTSKEEMQSMNEELQTVNTELQSNVDDLSWVNNDME HHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH NLLNSTEIATIFLDKGLHIRRFTNHATHLFKLIEGDVGRLLSDIVTDLDYKDLQKDAKEV HHHCCHHEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH LKRLAFVEKEITANNERCFKVRIMPYRTQENVIDGVVITFTNISEAKLLESELRKTRCKT HHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHCCEEEEEECCHHHHHHHHHHHHHCCCC >Mature Secondary Structure MINTPVIVGIGASAGGFEALEEFFSHVLTPCNIAFVVIQHLDPTHKGMMPELLQRDTTMK CCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHEECCCCCCCCCCHHHHCCCCHHH VKQARNNMKVKPNCVYVIPPDKDLSVLHGVLFLLEPVVKHTIRLPIDSFFQSLAADQHER HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHH AVGVILSGMGSDGTIGLRAIKENAGLSLVQSPESAKFDSMPQSAINAGLADITAPAEELP HHHHHEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHCCHHHCCCCHHHHH ERIIAFLEHGRRGVPNKLEPIVKRKSISALAQIMMILRERNGNDFSLYKTNTIDRRIERR HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHH MGLHQLKTISLYARYLRDNPQEQDLLFKELLIGVTNFFRDKDVWAQLKSTSLPALLANYP HCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCC EGKELRAWVTACSSGEEAYSLAITVMDVLDEIKPKGHFTLQIFATDLNQDAINIARKGYY CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHCCCC PAGIEADMSAQQLQRYFIKDGSGYRINKKIRDMVIFAPQNIIMDPPFTRLDILTCRNLLI CCCCCCCCCHHHHHHHHHCCCCCCEECHHHHCEEEECCCCEECCCCCCHHHHHHHHHHHH YFAPELQKKLLPLFHYTLTSHGLLILGHSETIGNATSLFSEIKENTRIYTRIDRPGQQME HCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEE VNFPTKIFPIMPLAENEHEGTRKMTKNISNLQTQADQILLQNYSPAAVLVNAAGDIIYIN ECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEE GRTGKYLEPAAGKANWNIHVMAREELQHQLELAIKKAQIQVEPVTIENITVDGHSVNLTV CCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCEEEEEE QAIIKPKELLGLIMVVFTEVVRPTRRRRKKLGAVEQESQAELQMAHDEIKSLREQMQSSH EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH EELKSANEELQSTNEELQSTNEELTTSKEEMQSMNEELQTVNTELQSNVDDLSWVNNDME HHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH NLLNSTEIATIFLDKGLHIRRFTNHATHLFKLIEGDVGRLLSDIVTDLDYKDLQKDAKEV HHHCCHHEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH LKRLAFVEKEITANNERCFKVRIMPYRTQENVIDGVVITFTNISEAKLLESELRKTRCKT HHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHCCEEEEEECCHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: Ca2+; Mg2+ [C]
Kcat value (1/min): 10 [C]
Specific activity: NA
Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]
Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]
Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade
General reaction: Methyl group transfer [C]
Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190 [H]