Click here to switch to the map view.

The map label for this gene is pyrF

Identifier: 119946630

GI number: 119946630

Start: 3709462

End: 3710166

Strand: Reverse

Name: pyrF

Synonym: Ping_3009

Alternate gene names: 119946630

Gene position: 3710166-3709462 (Counterclockwise)

Preceding gene: 119946631

Following gene: 119946629

Centisome position: 81.37

GC content: 42.7

Gene sequence:

>705_bases
ATGAATCAGTTAGTAGATCCTAAAGTCGTTATTGCATTAGATTATCAGGATAAACAGCAGGCATTGCACTTTATCAGCCA
GTTGGATCCAAAACATTGCCGCTTAAAAGTTGGTAAAGAGATGTTTACCCATTTTGGTCCTGAATTTGTTAAGCAGTTAG
TTGCCAAGGAGTTTGATGTTTTTTTAGATCTCAAATTTCACGATATTCCCAATACCGTTGCCAGTGCTGTTAAAGTGGCT
GCTGATTTAGGTGTGTGGATGGTGAATGTGCATGCCAGCGGAGGACGGCGCATGATGGAGGCGGCAAAAAAAATTCTAGA
GCCTTATGGTACTAATGCACCGCTATTAATTGCAGTGACAGTGTTAACCAGTATGGATCAAAGCGACTTACAAGAGTTGG
GTATTGAATTATCCCCCGCAGAGCAGGTAAAGCGTCTCGCTAAGCTGGCTAAATTAAGCGGTCTGGATGGTGTCGTATGT
TCGGCCCATGAAGCGCAGGAATTAAAAACCTTATTAGGTGCTGAGTTTAAGTTAATAACACCAGGAATTCGTCCAATGGG
CTCTGATGCCGGTGATCAGCGCCGAATTATGACACCCAAGCAAGCAATTGATGCCGGTTCTGATTATCTGGTTATTGGCC
GACCAATCACACGGGCGACAAATCCTGTTGAAGTATTAAAAAATATTAATGACAGTCTAGTTTGA

Upstream 100 bases:

>100_bases
AATGAATGCTTAAAGGCTGGAGCGCTGGAAAGTAGGAAGGCTGGAACGCCTTCTAGCTTTCCAGCCTTCTCGCCTTAATA
TGTAAATTATAGGAAATAGA

Downstream 100 bases:

>100_bases
CGGTGTTGCGTGACAAGCACATATCTTCCACCATTAACTTGGAATTGTTAAATGAAAAAGTTTGATATGAATAAATATAA
AATTGAATTTATGGAAAAAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MNQLVDPKVVIALDYQDKQQALHFISQLDPKHCRLKVGKEMFTHFGPEFVKQLVAKEFDVFLDLKFHDIPNTVASAVKVA
ADLGVWMVNVHASGGRRMMEAAKKILEPYGTNAPLLIAVTVLTSMDQSDLQELGIELSPAEQVKRLAKLAKLSGLDGVVC
SAHEAQELKTLLGAEFKLITPGIRPMGSDAGDQRRIMTPKQAIDAGSDYLVIGRPITRATNPVEVLKNINDSLV

Sequences:

>Translated_234_residues
MNQLVDPKVVIALDYQDKQQALHFISQLDPKHCRLKVGKEMFTHFGPEFVKQLVAKEFDVFLDLKFHDIPNTVASAVKVA
ADLGVWMVNVHASGGRRMMEAAKKILEPYGTNAPLLIAVTVLTSMDQSDLQELGIELSPAEQVKRLAKLAKLSGLDGVVC
SAHEAQELKTLLGAEFKLITPGIRPMGSDAGDQRRIMTPKQAIDAGSDYLVIGRPITRATNPVEVLKNINDSLV
>Mature_234_residues
MNQLVDPKVVIALDYQDKQQALHFISQLDPKHCRLKVGKEMFTHFGPEFVKQLVAKEFDVFLDLKFHDIPNTVASAVKVA
ADLGVWMVNVHASGGRRMMEAAKKILEPYGTNAPLLIAVTVLTSMDQSDLQELGIELSPAEQVKRLAKLAKLSGLDGVVC
SAHEAQELKTLLGAEFKLITPGIRPMGSDAGDQRRIMTPKQAIDAGSDYLVIGRPITRATNPVEVLKNINDSLV

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=66.2222222222222, Blast_Score=311, Evalue=2e-86,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PSYIN (A1SYZ6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944310.1
- ProteinModelPortal:   A1SYZ6
- SMR:   A1SYZ6
- STRING:   A1SYZ6
- GeneID:   4623793
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3009
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   A1SYZ6
- BioCyc:   PING357804:PING_3009-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25651; Mature: 25651

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 65-65 BINDING 14-14 BINDING 36-36 BINDING 123-123 BINDING 184-184 BINDING 193-193 BINDING 213-213 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLVDPKVVIALDYQDKQQALHFISQLDPKHCRLKVGKEMFTHFGPEFVKQLVAKEFDV
CCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLDLKFHDIPNTVASAVKVAADLGVWMVNVHASGGRRMMEAAKKILEPYGTNAPLLIAVT
EEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VLTSMDQSDLQELGIELSPAEQVKRLAKLAKLSGLDGVVCSAHEAQELKTLLGAEFKLIT
HHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCEEEEC
PGIRPMGSDAGDQRRIMTPKQAIDAGSDYLVIGRPITRATNPVEVLKNINDSLV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCHHHHHHHCHHCCC
>Mature Secondary Structure
MNQLVDPKVVIALDYQDKQQALHFISQLDPKHCRLKVGKEMFTHFGPEFVKQLVAKEFDV
CCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLDLKFHDIPNTVASAVKVAADLGVWMVNVHASGGRRMMEAAKKILEPYGTNAPLLIAVT
EEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VLTSMDQSDLQELGIELSPAEQVKRLAKLAKLSGLDGVVCSAHEAQELKTLLGAEFKLIT
HHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCEEEEC
PGIRPMGSDAGDQRRIMTPKQAIDAGSDYLVIGRPITRATNPVEVLKNINDSLV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCHHHHHHHCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA