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The map label for this gene is rbsB [H]

Identifier: 119946430

GI number: 119946430

Start: 3449259

End: 3450200

Strand: Reverse

Name: rbsB [H]

Synonym: Ping_2804

Alternate gene names: 119946430

Gene position: 3450200-3449259 (Counterclockwise)

Preceding gene: 119946431

Following gene: 119946429

Centisome position: 75.67

GC content: 41.83

Gene sequence:

>942_bases
ATGAAAAATATCATTAGCAAGATTATGATCGCTGGTATTGTGTTGTTGGGAAGCGTCACCTCTAAAGCCTTTGCTTTAAC
GGTTGCAATCAGTTTGGTGGATCTGTCCAATCCTTTTTTTATTATGCTTGCCGAAGAGATTTCTGCACAGTTAGAGGCTC
AGGTTGAAGGCCAGTTAACTATTTATGTGCATTCAAGTGCCTACGATCTTAATCAGCAAAACCAGCAAATTAAAGATTTT
ATTAACAAAGAGGTGGATATCATTTTCATCTCTGCTTCTGCCAGTGATGCTATTGAACCGAGTATAATAAGCGCTCGCGA
AAAAGGAATAATAGTCGCCGCAGTAGATATTGAATCACGGGGGGCTGATATCAGCATTACCTCTGATAATTTTCAGGCGG
GTCAGTTATCCTGCCAATATCTAGTGGATAGGCTTGGGGGAAAGGGTGAAATTGCAATTATCAATGGTTGGACAACAATA
TCATCAACCAGTAATCGTGTCGCAGGCTGCCGTGATCTATTAAAAAAATATCCCGGAATCAAGCTTGTTTCATACTCTCA
CAATGGCAGCGGAAGTTTCAGTGGCGGACTGGAAAGTATGACTTATCTTATGCTCGAATATCCGAATCTAGAGGCGGTAT
TTGCCATTAATGATCTCGCCGCCTTAGGCGCAGAAGAAGCGGCCAAACAGTCCGGAAACAATAAGATCATAATAGCTTCT
GTGGATGGCTCCCCTTTGTTCTTAGAACACCTTAAATTAAAAGACAGTCTATTAGTCGCCACCGCTGCACAGTTTCCTCG
CGCGATAGCCCAAAAAGCAGTTATTCTCGCGTTACAAAAACTCAGAGAAACATCCGCAGGCCATACGACAGAGTTAATAC
CTACTGAATTAGTGACCAAACAGAATGTCAGTCAATTTAGCAACTGGGATAAAACAAAATGA

Upstream 100 bases:

>100_bases
GTAAGATTGAATGTAATTTTATTAATAAACCAGTGACTTATAAAGACATAGCTAACATCATTATTGGTACAAATGCAGAT
AATGACGTAAAAGGAGAGGT

Downstream 100 bases:

>100_bases
AAGATTATGACCAACTTGCCGATCAGCTACGCGACAAATCATTAAAATTAAGGGCTGGAGCGCAATACTGGATTGCATTA
GCAGGCGCTCCGGGCAGTGG

Product: transcriptional regulator, periplasmic binding protein of LacI family protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKNIISKIMIAGIVLLGSVTSKAFALTVAISLVDLSNPFFIMLAEEISAQLEAQVEGQLTIYVHSSAYDLNQQNQQIKDF
INKEVDIIFISASASDAIEPSIISAREKGIIVAAVDIESRGADISITSDNFQAGQLSCQYLVDRLGGKGEIAIINGWTTI
SSTSNRVAGCRDLLKKYPGIKLVSYSHNGSGSFSGGLESMTYLMLEYPNLEAVFAINDLAALGAEEAAKQSGNNKIIIAS
VDGSPLFLEHLKLKDSLLVATAAQFPRAIAQKAVILALQKLRETSAGHTTELIPTELVTKQNVSQFSNWDKTK

Sequences:

>Translated_313_residues
MKNIISKIMIAGIVLLGSVTSKAFALTVAISLVDLSNPFFIMLAEEISAQLEAQVEGQLTIYVHSSAYDLNQQNQQIKDF
INKEVDIIFISASASDAIEPSIISAREKGIIVAAVDIESRGADISITSDNFQAGQLSCQYLVDRLGGKGEIAIINGWTTI
SSTSNRVAGCRDLLKKYPGIKLVSYSHNGSGSFSGGLESMTYLMLEYPNLEAVFAINDLAALGAEEAAKQSGNNKIIIAS
VDGSPLFLEHLKLKDSLLVATAAQFPRAIAQKAVILALQKLRETSAGHTTELIPTELVTKQNVSQFSNWDKTK
>Mature_313_residues
MKNIISKIMIAGIVLLGSVTSKAFALTVAISLVDLSNPFFIMLAEEISAQLEAQVEGQLTIYVHSSAYDLNQQNQQIKDF
INKEVDIIFISASASDAIEPSIISAREKGIIVAAVDIESRGADISITSDNFQAGQLSCQYLVDRLGGKGEIAIINGWTTI
SSTSNRVAGCRDLLKKYPGIKLVSYSHNGSGSFSGGLESMTYLMLEYPNLEAVFAINDLAALGAEEAAKQSGNNKIIIAS
VDGSPLFLEHLKLKDSLLVATAAQFPRAIAQKAVILALQKLRETSAGHTTELIPTELVTKQNVSQFSNWDKTK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=300, Percent_Identity=27, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790526, Length=264, Percent_Identity=29.5454545454545, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787906, Length=281, Percent_Identity=26.6903914590747, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790674, Length=217, Percent_Identity=28.110599078341, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33669; Mature: 33669

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIISKIMIAGIVLLGSVTSKAFALTVAISLVDLSNPFFIMLAEEISAQLEAQVEGQLT
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEHHHHHHHEEEECCEEE
IYVHSSAYDLNQQNQQIKDFINKEVDIIFISASASDAIEPSIISAREKGIIVAAVDIESR
EEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEEEEECCC
GADISITSDNFQAGQLSCQYLVDRLGGKGEIAIINGWTTISSTSNRVAGCRDLLKKYPGI
CCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCHHHHHHHHHHHCCCE
KLVSYSHNGSGSFSGGLESMTYLMLEYPNLEAVFAINDLAALGAEEAAKQSGNNKIIIAS
EEEEECCCCCCCCCCCHHHHEEEEEECCCCEEEEEEHHHHHCCHHHHHHHCCCCEEEEEE
VDGSPLFLEHLKLKDSLLVATAAQFPRAIAQKAVILALQKLRETSAGHTTELIPTELVTK
CCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHH
QNVSQFSNWDKTK
HHHHHHCCCCCCC
>Mature Secondary Structure
MKNIISKIMIAGIVLLGSVTSKAFALTVAISLVDLSNPFFIMLAEEISAQLEAQVEGQLT
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEHHHHHHHEEEECCEEE
IYVHSSAYDLNQQNQQIKDFINKEVDIIFISASASDAIEPSIISAREKGIIVAAVDIESR
EEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEEEEECCC
GADISITSDNFQAGQLSCQYLVDRLGGKGEIAIINGWTTISSTSNRVAGCRDLLKKYPGI
CCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCHHHHHHHHHHHCCCE
KLVSYSHNGSGSFSGGLESMTYLMLEYPNLEAVFAINDLAALGAEEAAKQSGNNKIIIAS
EEEEECCCCCCCCCCCHHHHEEEEEECCCCEEEEEEHHHHHCCHHHHHHHCCCCEEEEEE
VDGSPLFLEHLKLKDSLLVATAAQFPRAIAQKAVILALQKLRETSAGHTTELIPTELVTK
CCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHH
QNVSQFSNWDKTK
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]