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The map label for this gene is ldhA [C]

Identifier: 119946420

GI number: 119946420

Start: 3439115

End: 3440041

Strand: Reverse

Name: ldhA [C]

Synonym: Ping_2794

Alternate gene names: 119946420

Gene position: 3440041-3439115 (Counterclockwise)

Preceding gene: 119946421

Following gene: 119946416

Centisome position: 75.45

GC content: 38.4

Gene sequence:

>927_bases
ATGAAAATTGTATTATTGGATGCAGAAACATTAAGTGATAGTGATTTATCTCCATTTGAAAATTTGGGTGATTTTATAAG
CTATCCGCTGACCAGCAAGGAACAAAGATTATTACATATTGGCGACCGGAATGTCATTATCACCAATAAGGTTGTTATTG
ATCAGGAAATTATAGCGGCAAATCCACAATTGAAATTAATCTGTATTGCAGCTACGGGAATGAACAATGTTGATTTAATT
GCTGCTGAAAAAGCCGGTATAATGGTTAAAAATGTGTCTGGCTATTCTACCGAGAGTGTCGCCCAATCTACTTTTTCAAT
GCTCTTTCAGTTAATACATCAAAGCCGTTATTATGATCATTACACACAGTCTAAAGAATGGTGTAACAGCCCCACCTTTA
CGCATCTCAGCAAACCCTTTGGTGAAATAAAGGGTAAACGTTGGGGCATTATCGGCATGGGCGAAATTGGTCGACGAGTT
GCTGAAATTGCAAGCGTATTTGGATGTGAACTTTGTTATTTTTCTGCTTCGGGTAAAAATAATCAGCAAGATTATCCGCA
GGTTGATTTGGCAACGTTATTATCTACCTGTGACATTATTAGTATTCATGCACCGCTTAATGAACATACGCATAATTTAA
TTGATAAACAGGCGCTAAAAAAACTAAAAGCAGGGGCTGTTCTACTTAATATGGGCCGCGGTGGGATAGTCAATGAAGAT
GCAATGGCTGAAGCGCTTGATCAGTCGGATATTTATCATGGTACTGATGTGCTCGAGATTGAGCCTATGATTCAAGACCA
TCCCTATTTATCTGTGCAGGCAAAAGAGCGCTTGGTGATGACTCCCCATACTGCATGGGCCAGTCAAGAAGCAAGAACAA
AACTTATCAATATGATTGTTGATAATATTAAATCTGCGAATTTTTAG

Upstream 100 bases:

>100_bases
TGATTAACTTCTATGTTATTTTTTTGAGTCTATTTATTAGGCACCCAAACCTAATGGAGCTTCAGGTTTGGGGTTATCAA
TATTAACATTAGGGAAGAGT

Downstream 100 bases:

>100_bases
CATTGGTCTTAATCTGTAGCAGCTCATTTATTGAGTTATAAAGATTGTTACAGCAGAAAAATAAAAAACACCCTTTATGC
AAATAAAGGGTGTTTTTTTT

Product: 2-hydroxyacid dehydrogenase

Products: H+; Nicotinamide adenine dinucleotide - reduced; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKIVLLDAETLSDSDLSPFENLGDFISYPLTSKEQRLLHIGDRNVIITNKVVIDQEIIAANPQLKLICIAATGMNNVDLI
AAEKAGIMVKNVSGYSTESVAQSTFSMLFQLIHQSRYYDHYTQSKEWCNSPTFTHLSKPFGEIKGKRWGIIGMGEIGRRV
AEIASVFGCELCYFSASGKNNQQDYPQVDLATLLSTCDIISIHAPLNEHTHNLIDKQALKKLKAGAVLLNMGRGGIVNED
AMAEALDQSDIYHGTDVLEIEPMIQDHPYLSVQAKERLVMTPHTAWASQEARTKLINMIVDNIKSANF

Sequences:

>Translated_308_residues
MKIVLLDAETLSDSDLSPFENLGDFISYPLTSKEQRLLHIGDRNVIITNKVVIDQEIIAANPQLKLICIAATGMNNVDLI
AAEKAGIMVKNVSGYSTESVAQSTFSMLFQLIHQSRYYDHYTQSKEWCNSPTFTHLSKPFGEIKGKRWGIIGMGEIGRRV
AEIASVFGCELCYFSASGKNNQQDYPQVDLATLLSTCDIISIHAPLNEHTHNLIDKQALKKLKAGAVLLNMGRGGIVNED
AMAEALDQSDIYHGTDVLEIEPMIQDHPYLSVQAKERLVMTPHTAWASQEARTKLINMIVDNIKSANF
>Mature_308_residues
MKIVLLDAETLSDSDLSPFENLGDFISYPLTSKEQRLLHIGDRNVIITNKVVIDQEIIAANPQLKLICIAATGMNNVDLI
AAEKAGIMVKNVSGYSTESVAQSTFSMLFQLIHQSRYYDHYTQSKEWCNSPTFTHLSKPFGEIKGKRWGIIGMGEIGRRV
AEIASVFGCELCYFSASGKNNQQDYPQVDLATLLSTCDIISIHAPLNEHTHNLIDKQALKKLKAGAVLLNMGRGGIVNED
AMAEALDQSDIYHGTDVLEIEPMIQDHPYLSVQAKERLVMTPHTAWASQEARTKLINMIVDNIKSANF

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580575, Length=273, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4557497, Length=280, Percent_Identity=29.2857142857143, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI145580578, Length=255, Percent_Identity=30.1960784313725, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4557499, Length=255, Percent_Identity=30.1960784313725, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI61743967, Length=280, Percent_Identity=29.2857142857143, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI23308577, Length=277, Percent_Identity=29.6028880866426, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI6912396, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787645, Length=257, Percent_Identity=30.3501945525292, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082289, Length=263, Percent_Identity=27.3764258555133, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=252, Percent_Identity=28.1746031746032, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87081824, Length=187, Percent_Identity=28.8770053475936, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=272, Percent_Identity=29.0441176470588, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25147481, Length=233, Percent_Identity=24.4635193133047, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324055, Length=334, Percent_Identity=27.5449101796407, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=277, Percent_Identity=26.7148014440433, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320925, Length=315, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322116, Length=315, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24646446, Length=268, Percent_Identity=29.8507462686567, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646448, Length=268, Percent_Identity=29.8507462686567, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646452, Length=268, Percent_Identity=29.8507462686567, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646450, Length=268, Percent_Identity=29.8507462686567, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI62472511, Length=268, Percent_Identity=29.8507462686567, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574286, Length=287, Percent_Identity=29.616724738676, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585514, Length=255, Percent_Identity=27.0588235294118, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28574282, Length=255, Percent_Identity=27.0588235294118, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28574284, Length=255, Percent_Identity=27.0588235294118, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45551003, Length=252, Percent_Identity=26.5873015873016, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45552429, Length=255, Percent_Identity=27.0588235294118, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28571528, Length=272, Percent_Identity=28.6764705882353, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19921140, Length=262, Percent_Identity=24.8091603053435, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585516, Length=253, Percent_Identity=24.5059288537549, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28 [C]

Molecular weight: Translated: 34184; Mature: 34184

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLLDAETLSDSDLSPFENLGDFISYPLTSKEQRLLHIGDRNVIITNKVVIDQEIIAA
CEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEECHHHHCC
NPQLKLICIAATGMNNVDLIAAEKAGIMVKNVSGYSTESVAQSTFSMLFQLIHQSRYYDH
CCCEEEEEEEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YTQSKEWCNSPTFTHLSKPFGEIKGKRWGIIGMGEIGRRVAEIASVFGCELCYFSASGKN
HHCCHHHCCCCCHHHHHCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCC
NQQDYPQVDLATLLSTCDIISIHAPLNEHTHNLIDKQALKKLKAGAVLLNMGRGGIVNED
CCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHH
AMAEALDQSDIYHGTDVLEIEPMIQDHPYLSVQAKERLVMTPHTAWASQEARTKLINMIV
HHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCEEEECCCHHHCCHHHHHHHHHHHH
DNIKSANF
HHHHCCCC
>Mature Secondary Structure
MKIVLLDAETLSDSDLSPFENLGDFISYPLTSKEQRLLHIGDRNVIITNKVVIDQEIIAA
CEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEECHHHHCC
NPQLKLICIAATGMNNVDLIAAEKAGIMVKNVSGYSTESVAQSTFSMLFQLIHQSRYYDH
CCCEEEEEEEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YTQSKEWCNSPTFTHLSKPFGEIKGKRWGIIGMGEIGRRVAEIASVFGCELCYFSASGKN
HHCCHHHCCCCCHHHHHCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCC
NQQDYPQVDLATLLSTCDIISIHAPLNEHTHNLIDKQALKKLKAGAVLLNMGRGGIVNED
CCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHH
AMAEALDQSDIYHGTDVLEIEPMIQDHPYLSVQAKERLVMTPHTAWASQEARTKLINMIV
HHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCEEEECCCHHHCCHHHHHHHHHHHH
DNIKSANF
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Lactate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Lactate + Nicotinamide adenine dinucleotide <==> H+ + Nicotinamide adenine dinucleotide - reduced + Pyruvate [C]

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]