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The map label for this gene is ppdK [H]

Identifier: 119946388

GI number: 119946388

Start: 3388349

End: 3391009

Strand: Reverse

Name: ppdK [H]

Synonym: Ping_2762

Alternate gene names: 119946388

Gene position: 3391009-3388349 (Counterclockwise)

Preceding gene: 119946389

Following gene: 119946387

Centisome position: 74.37

GC content: 46.15

Gene sequence:

>2661_bases
ATGGCAAAATATGTTTATTTATTTACTAAAGGCCAAGCCGACGGTGATCGCTCACAACGCGAGTTACTTGGTGGAAAGGG
GGCTAATCTCGCTGAAATGGCTGCAATCGGCCTGCCCGTTCCTTCCGGATTCACTTTAACAACTGAAGTCTGTAAGTCAT
TTTATCAACACGATCGTCATTACCCTGAAGGAGTGGAGTCGCAGGTTAATAATGCACTTAAAAAGCTTGAGCAGGAGATG
GCAAAAGGTTTTGGTGATCCGGTAAACCCTTTATTGTTATCAGTACGCTCCGGTGCGCCTACCTCTATGCCCGGCATGAT
GGACACCGTACTTAATTTGGGTCTCAATGACCAGACGGTGCAGGGACTTATTAACCTGAGCGGCTCACCGCGTTTTGCCT
ATGACTCATATCGGCGCTTTATCCAGATGTACGCTAATGTTGTTAAGGGAGTAGATGGTGATATTTTTGAAACGTTATTA
GAGGATAAAAAACGCGCGAAAGGTATTGAAAATGATAACGAGTTGAGTGCGGAAGACCTGCAGCAGTTAATTACCGAATT
CAAAGACAGCTATCGGCAAGTGCTTAATCTTGATTTTCCAACTGATCCGCAGCAGCAACTTTGGGAGGCTATTAGTGCTG
TATTTGGATCTTGGATGAATCAGCGAGCGGTAACTTATCGCAGCTTAAATAACATTCCATCCCATTGGGGAACAGCTGTC
AATGTGCAGGCCATGGTATTTGGCAATATGGGCAATGATTGTGCGACCGGCGTTGCCTTCACGCGTGATCCCTCAACAGG
AGAAAATTATTTTTATGGCGAATTTTTAGTCAATGCTCAGGGGGAAGATGTTGTTGCTGGGATCCGGACACCACAACCTA
TCAATAAGGCTAAATATAAACCGGGCGACTTGCCGGCATTGGAGGATGTTTTGCCGGGGTGTTACGCACAGTTATTGGAT
CACCGTAATACGCTGGAAAAACATTTTCGTGATATGCAGGATATTGAGTTCACTATTGAATCTGGCAAACTGTATATGCT
ACAAACCCGAACGGGTAAAAGAACTGCCCAAGCTGCGATCCGGATTGCTGTGGAGATGGTTGGTGAGAAACTGATCAGTA
AAAAAGAGGCAGTATTAAGAGTCTTACCGGAACAGCTCGAGCAATTACTACACCCTTCTATTGACCCTTCTGTTGTCAAA
AATATAATCGCCAAGGGTTTACCCGCCTCTCCTGGTGCTTGTAGTGGCAAGGTGGTCTTTAATGCCGATGAAGCTGAACA
ACAAAACAAATTGAAAGAGAAAGTCATTCTTGTTCGGATTGAAACCAGCCCTGAGGATATTCATGGTATGAACGCCGCAC
AGGGCATTCTAACCTCGCGTGGCGGAATGACGTCACATGCGGCTGTTGTCGCTCGGGGGATGGGTAAAAGCTGCGTTGCA
GGTTGTGGAGATCTGCATATTGATTACGAAAAACAGGTAATGGTGACTAAGTCGGGACATACCATCAAGCGCGGGGATAT
TATTACCCTGGATGGAGCAGCTGGTGAAGTTATTCTGGGTGATGTAGCAAAAGTACAACCCGCCGTCAGTGGAGACTTTG
GTACATTAATGAGTTGGGCCGACACATTCCGTACACTTAATGTAAGAGCCAATGCAGATACTCCTCATGATGCCAAGATT
GCGCGAGAATTTGGCGCTGAGGGAATAGGTTTATGTCGTACTGAACATATGTTTTTCGATGTTAAACGGATCCAAGCGGT
GCGTGAAATGATTGTGTCAGCCGATCTGCAAGGTCGGGAAAGGGCGCTTTCTAAAATCATGCCGATGCAGAAAAGTGATT
TTATCGGGCTGTTCCGCGAGATGAGAGGGGTGCCTGTCACCATTCGATTACTGGATCCTCCACTGCACGAATTTTTACCA
CAGAATAACGATGATATTGCCGATCTTGCGGCCCTTGCCGGAATGCGTTTCTCGGAAGTGAAAGAGCATGTTGAAGAACT
GCATGAATTCAATCCGATGCTCGGTCATCGTGGTTGTCGACTCGGTCTTACTTATCCGGAGGTTTACGATATGCAGGTGC
GAGCTATTATGGAAGCCGCTTGTGAACTGGTTAAAAATGAGCATTATAAAATTGTTCCGGAGATCATGATTCCGCTTGTG
GCTGATGTTAATGAGTTGAAGGTGCTGCGCGAAAAAGCGATCCAGGTAGCAGAACAGGTAATGGCTACTTATGGCGTTAA
AGTTGACTACCAAATCGGCACTATGATTGAATTGCCACGGGCAGCCCTATTGGCTGATCTGATTGCAGAGCAAGCTGATT
TCTTTTCCTTTGGCACTAATGATCTCACTCAGACTACCTATGGTTTGTCGCGCGATGATGCTGGACGCTTTTTGCCTTAT
TATATAGAAAATGGGCTATTTTTAAGTGATCCCTTTATTAAATTGGATCGTGCCGGGGTTGGCCAACTGGTAAAAATGGG
CACTGAAAAGGGCAGACAGACCCGGCCGGGTTTGAAAGTTGGCATCTGCGGAGAGCATGGTGGAGAGCCTTCTAGTGTTG
AATTTTGTCATCAAATTGGTCTCGATTATGTTTCCTGTTCGCCATATCGTGTTCCCATTGCGCGCTTAGCTGCCGCCCAC
GCAGCTCTAAAGGATGAATAG

Upstream 100 bases:

>100_bases
ATAAACAGATCAAATAGTTAATAATTAATTCATCTTAAAATTACTTTTTTAAAATAGAGCGTGCTCTATTTTTCTGCTTA
TATGCTAAGAGGCAATGATC

Downstream 100 bases:

>100_bases
TAAATAGTCAATAACCTAAACTTAAAACGCTTGGTAATCTCTTTACCCGGCGTTTTTTGACAGTACTGAACGCTATAAAG
GCAATAATGGATATGTATAT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 886; Mature: 885

Protein sequence:

>886_residues
MAKYVYLFTKGQADGDRSQRELLGGKGANLAEMAAIGLPVPSGFTLTTEVCKSFYQHDRHYPEGVESQVNNALKKLEQEM
AKGFGDPVNPLLLSVRSGAPTSMPGMMDTVLNLGLNDQTVQGLINLSGSPRFAYDSYRRFIQMYANVVKGVDGDIFETLL
EDKKRAKGIENDNELSAEDLQQLITEFKDSYRQVLNLDFPTDPQQQLWEAISAVFGSWMNQRAVTYRSLNNIPSHWGTAV
NVQAMVFGNMGNDCATGVAFTRDPSTGENYFYGEFLVNAQGEDVVAGIRTPQPINKAKYKPGDLPALEDVLPGCYAQLLD
HRNTLEKHFRDMQDIEFTIESGKLYMLQTRTGKRTAQAAIRIAVEMVGEKLISKKEAVLRVLPEQLEQLLHPSIDPSVVK
NIIAKGLPASPGACSGKVVFNADEAEQQNKLKEKVILVRIETSPEDIHGMNAAQGILTSRGGMTSHAAVVARGMGKSCVA
GCGDLHIDYEKQVMVTKSGHTIKRGDIITLDGAAGEVILGDVAKVQPAVSGDFGTLMSWADTFRTLNVRANADTPHDAKI
AREFGAEGIGLCRTEHMFFDVKRIQAVREMIVSADLQGRERALSKIMPMQKSDFIGLFREMRGVPVTIRLLDPPLHEFLP
QNNDDIADLAALAGMRFSEVKEHVEELHEFNPMLGHRGCRLGLTYPEVYDMQVRAIMEAACELVKNEHYKIVPEIMIPLV
ADVNELKVLREKAIQVAEQVMATYGVKVDYQIGTMIELPRAALLADLIAEQADFFSFGTNDLTQTTYGLSRDDAGRFLPY
YIENGLFLSDPFIKLDRAGVGQLVKMGTEKGRQTRPGLKVGICGEHGGEPSSVEFCHQIGLDYVSCSPYRVPIARLAAAH
AALKDE

Sequences:

>Translated_886_residues
MAKYVYLFTKGQADGDRSQRELLGGKGANLAEMAAIGLPVPSGFTLTTEVCKSFYQHDRHYPEGVESQVNNALKKLEQEM
AKGFGDPVNPLLLSVRSGAPTSMPGMMDTVLNLGLNDQTVQGLINLSGSPRFAYDSYRRFIQMYANVVKGVDGDIFETLL
EDKKRAKGIENDNELSAEDLQQLITEFKDSYRQVLNLDFPTDPQQQLWEAISAVFGSWMNQRAVTYRSLNNIPSHWGTAV
NVQAMVFGNMGNDCATGVAFTRDPSTGENYFYGEFLVNAQGEDVVAGIRTPQPINKAKYKPGDLPALEDVLPGCYAQLLD
HRNTLEKHFRDMQDIEFTIESGKLYMLQTRTGKRTAQAAIRIAVEMVGEKLISKKEAVLRVLPEQLEQLLHPSIDPSVVK
NIIAKGLPASPGACSGKVVFNADEAEQQNKLKEKVILVRIETSPEDIHGMNAAQGILTSRGGMTSHAAVVARGMGKSCVA
GCGDLHIDYEKQVMVTKSGHTIKRGDIITLDGAAGEVILGDVAKVQPAVSGDFGTLMSWADTFRTLNVRANADTPHDAKI
AREFGAEGIGLCRTEHMFFDVKRIQAVREMIVSADLQGRERALSKIMPMQKSDFIGLFREMRGVPVTIRLLDPPLHEFLP
QNNDDIADLAALAGMRFSEVKEHVEELHEFNPMLGHRGCRLGLTYPEVYDMQVRAIMEAACELVKNEHYKIVPEIMIPLV
ADVNELKVLREKAIQVAEQVMATYGVKVDYQIGTMIELPRAALLADLIAEQADFFSFGTNDLTQTTYGLSRDDAGRFLPY
YIENGLFLSDPFIKLDRAGVGQLVKMGTEKGRQTRPGLKVGICGEHGGEPSSVEFCHQIGLDYVSCSPYRVPIARLAAAH
AALKDE
>Mature_885_residues
AKYVYLFTKGQADGDRSQRELLGGKGANLAEMAAIGLPVPSGFTLTTEVCKSFYQHDRHYPEGVESQVNNALKKLEQEMA
KGFGDPVNPLLLSVRSGAPTSMPGMMDTVLNLGLNDQTVQGLINLSGSPRFAYDSYRRFIQMYANVVKGVDGDIFETLLE
DKKRAKGIENDNELSAEDLQQLITEFKDSYRQVLNLDFPTDPQQQLWEAISAVFGSWMNQRAVTYRSLNNIPSHWGTAVN
VQAMVFGNMGNDCATGVAFTRDPSTGENYFYGEFLVNAQGEDVVAGIRTPQPINKAKYKPGDLPALEDVLPGCYAQLLDH
RNTLEKHFRDMQDIEFTIESGKLYMLQTRTGKRTAQAAIRIAVEMVGEKLISKKEAVLRVLPEQLEQLLHPSIDPSVVKN
IIAKGLPASPGACSGKVVFNADEAEQQNKLKEKVILVRIETSPEDIHGMNAAQGILTSRGGMTSHAAVVARGMGKSCVAG
CGDLHIDYEKQVMVTKSGHTIKRGDIITLDGAAGEVILGDVAKVQPAVSGDFGTLMSWADTFRTLNVRANADTPHDAKIA
REFGAEGIGLCRTEHMFFDVKRIQAVREMIVSADLQGRERALSKIMPMQKSDFIGLFREMRGVPVTIRLLDPPLHEFLPQ
NNDDIADLAALAGMRFSEVKEHVEELHEFNPMLGHRGCRLGLTYPEVYDMQVRAIMEAACELVKNEHYKIVPEIMIPLVA
DVNELKVLREKAIQVAEQVMATYGVKVDYQIGTMIELPRAALLADLIAEQADFFSFGTNDLTQTTYGLSRDDAGRFLPYY
IENGLFLSDPFIKLDRAGVGQLVKMGTEKGRQTRPGLKVGICGEHGGEPSSVEFCHQIGLDYVSCSPYRVPIARLAAAHA
ALKDE

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=459, Percent_Identity=25.9259259259259, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787994, Length=744, Percent_Identity=26.0752688172043, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI48994992, Length=454, Percent_Identity=25.5506607929515, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788726, Length=589, Percent_Identity=23.9388794567063, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1789193, Length=484, Percent_Identity=24.1735537190083, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97643; Mature: 97512

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYVYLFTKGQADGDRSQRELLGGKGANLAEMAAIGLPVPSGFTLTTEVCKSFYQHDRH
CCEEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHCCC
YPEGVESQVNNALKKLEQEMAKGFGDPVNPLLLSVRSGAPTSMPGMMDTVLNLGLNDQTV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCHHHHHHHHCCCCHHHH
QGLINLSGSPRFAYDSYRRFIQMYANVVKGVDGDIFETLLEDKKRAKGIENDNELSAEDL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
QQLITEFKDSYRQVLNLDFPTDPQQQLWEAISAVFGSWMNQRAVTYRSLNNIPSHWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEE
NVQAMVFGNMGNDCATGVAFTRDPSTGENYFYGEFLVNAQGEDVVAGIRTPQPINKAKYK
EEEEEEECCCCCHHHCCCEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCC
PGDLPALEDVLPGCYAQLLDHRNTLEKHFRDMQDIEFTIESGKLYMLQTRTGKRTAQAAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCEEEEEEECCCCHHHHHHH
RIAVEMVGEKLISKKEAVLRVLPEQLEQLLHPSIDPSVVKNIIAKGLPASPGACSGKVVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEE
NADEAEQQNKLKEKVILVRIETSPEDIHGMNAAQGILTSRGGMTSHAAVVARGMGKSCVA
CCHHHHHHHHHHHEEEEEEEECCCHHHCCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHC
GCGDLHIDYEKQVMVTKSGHTIKRGDIITLDGAAGEVILGDVAKVQPAVSGDFGTLMSWA
CCCCEEECCCCEEEEEECCCEEECCCEEEECCCCCCEEECCHHHHCCCCCCCHHHHHHHH
DTFRTLNVRANADTPHDAKIAREFGAEGIGLCRTEHMFFDVKRIQAVREMIVSADLQGRE
HHHHEEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
RALSKIMPMQKSDFIGLFREMRGVPVTIRLLDPPLHEFLPQNNDDIADLAALAGMRFSEV
HHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHCCHHHHH
KEHVEELHEFNPMLGHRGCRLGLTYPEVYDMQVRAIMEAACELVKNEHYKIVPEIMIPLV
HHHHHHHHHCCHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHH
ADVNELKVLREKAIQVAEQVMATYGVKVDYQIGTMIELPRAALLADLIAEQADFFSFGTN
HCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DLTQTTYGLSRDDAGRFLPYYIENGLFLSDPFIKLDRAGVGQLVKMGTEKGRQTRPGLKV
CHHHHHCCCCCCCCCCCCHHHHCCCEEECCCCCEECCCCHHHHHHHHHHCCCCCCCCCEE
GICGEHGGEPSSVEFCHQIGLDYVSCSPYRVPIARLAAAHAALKDE
EECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKYVYLFTKGQADGDRSQRELLGGKGANLAEMAAIGLPVPSGFTLTTEVCKSFYQHDRH
CEEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHCCC
YPEGVESQVNNALKKLEQEMAKGFGDPVNPLLLSVRSGAPTSMPGMMDTVLNLGLNDQTV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCHHHHHHHHCCCCHHHH
QGLINLSGSPRFAYDSYRRFIQMYANVVKGVDGDIFETLLEDKKRAKGIENDNELSAEDL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
QQLITEFKDSYRQVLNLDFPTDPQQQLWEAISAVFGSWMNQRAVTYRSLNNIPSHWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEE
NVQAMVFGNMGNDCATGVAFTRDPSTGENYFYGEFLVNAQGEDVVAGIRTPQPINKAKYK
EEEEEEECCCCCHHHCCCEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCC
PGDLPALEDVLPGCYAQLLDHRNTLEKHFRDMQDIEFTIESGKLYMLQTRTGKRTAQAAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCEEEEEEECCCCHHHHHHH
RIAVEMVGEKLISKKEAVLRVLPEQLEQLLHPSIDPSVVKNIIAKGLPASPGACSGKVVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEE
NADEAEQQNKLKEKVILVRIETSPEDIHGMNAAQGILTSRGGMTSHAAVVARGMGKSCVA
CCHHHHHHHHHHHEEEEEEEECCCHHHCCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHC
GCGDLHIDYEKQVMVTKSGHTIKRGDIITLDGAAGEVILGDVAKVQPAVSGDFGTLMSWA
CCCCEEECCCCEEEEEECCCEEECCCEEEECCCCCCEEECCHHHHCCCCCCCHHHHHHHH
DTFRTLNVRANADTPHDAKIAREFGAEGIGLCRTEHMFFDVKRIQAVREMIVSADLQGRE
HHHHEEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
RALSKIMPMQKSDFIGLFREMRGVPVTIRLLDPPLHEFLPQNNDDIADLAALAGMRFSEV
HHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHCCHHHHH
KEHVEELHEFNPMLGHRGCRLGLTYPEVYDMQVRAIMEAACELVKNEHYKIVPEIMIPLV
HHHHHHHHHCCHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHH
ADVNELKVLREKAIQVAEQVMATYGVKVDYQIGTMIELPRAALLADLIAEQADFFSFGTN
HCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DLTQTTYGLSRDDAGRFLPYYIENGLFLSDPFIKLDRAGVGQLVKMGTEKGRQTRPGLKV
CHHHHHCCCCCCCCCCCCHHHHCCCEEECCCCCEECCCCHHHHHHHHHHCCCCCCCCCEE
GICGEHGGEPSSVEFCHQIGLDYVSCSPYRVPIARLAAAHAALKDE
EECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]