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The map label for this gene is ywbI [H]

Identifier: 119946364

GI number: 119946364

Start: 3356552

End: 3357436

Strand: Reverse

Name: ywbI [H]

Synonym: Ping_2736

Alternate gene names: 119946364

Gene position: 3357436-3356552 (Counterclockwise)

Preceding gene: 119946368

Following gene: 119946363

Centisome position: 73.63

GC content: 41.58

Gene sequence:

>885_bases
ATGGATTTTAAAAGACTGTATTATTTTACTGTATTAGCGCAAACCGGAAACTTTACTAAAGCTGCTGAGGAATTAGGGAT
TGCACAGTCAGCATTGAGTATGAGTATTCAGAAGTTGGAGAAACAACTGGCACTGAAGCTGATTAATAGAGCTGAACGCA
AGATGAGTATTACCAGTGATGGGCAAGTATTGCTGAAGCATGCGCAGAAAATTTTAGACAATGTTAAACAAGCAAAAATA
CAACTGCAAGAACTTAAGGGACTTGAGTCCGGCACCATCAACCTCGGTACTCCTGCGATGATCGGCTCCTACTATCTGCC
TGATACGCTGGCATTATTTAAACAGCGCTACCCGGGTATTCAAATTAATATTCATGAAGCGGGTACGGCGACTTTAGCCA
ATATGCTGCTTGATGGTGAATTAGATCTGGCGATGATCCGCGCCGATAGACACCATGACAATATTCGCTGTATTACCTTA
GCTAAAGAGCAAATAGTGGCTTGTGTACCGCGGGAACATGAATTTGCCAAATGCTATAACATCACACTCGAGCAATTTTG
CAACCAGCCTTTGGTTCTGTTCCGTGAAGGTTATTTTCTGCGCGAAGCGGTGAGCAGTTATTGCCAGCAACATGGAATTA
ATCCTGATATTCGTTTTGAAACGAATTTAATTGAATTATTGAAATCGTTAATCCGCAAGAAAATAGGTATCTCGACTTGT
TTACCTATTATTTTGACCGAAGATAGTGAGCTTGTGACTGTGCCTTTTAATCCCCCTATTCCAATACATTTGGGGATAGG
GTGGAAAGCAAGTCACTACTTATCCCAAGCATCCCAAGCATTGGTTACATTTCTACAAGAAGAAATGGATGGCGGACAGG
GCTAA

Upstream 100 bases:

>100_bases
CCCCTGCTGTGTTGATCCGTGACGCTATAGTCGAGCTACAGTATTGATAATTTAACCGCTGTGTCGTAGCAGTATTGAAA
TGTAATAAGGACCTTGAGTA

Downstream 100 bases:

>100_bases
TGTTTGTCTGACTTCCCGTTAGTGCATCACTAACGGGAAGTTAGAAGCTATTATTGCGCCTTTGGTAATATAGAAAGATC
ACTTGGCCTTTATTTCTACA

Product: transcriptional regulator, substrate-binding of LysR family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDFKRLYYFTVLAQTGNFTKAAEELGIAQSALSMSIQKLEKQLALKLINRAERKMSITSDGQVLLKHAQKILDNVKQAKI
QLQELKGLESGTINLGTPAMIGSYYLPDTLALFKQRYPGIQINIHEAGTATLANMLLDGELDLAMIRADRHHDNIRCITL
AKEQIVACVPREHEFAKCYNITLEQFCNQPLVLFREGYFLREAVSSYCQQHGINPDIRFETNLIELLKSLIRKKIGISTC
LPIILTEDSELVTVPFNPPIPIHLGIGWKASHYLSQASQALVTFLQEEMDGGQG

Sequences:

>Translated_294_residues
MDFKRLYYFTVLAQTGNFTKAAEELGIAQSALSMSIQKLEKQLALKLINRAERKMSITSDGQVLLKHAQKILDNVKQAKI
QLQELKGLESGTINLGTPAMIGSYYLPDTLALFKQRYPGIQINIHEAGTATLANMLLDGELDLAMIRADRHHDNIRCITL
AKEQIVACVPREHEFAKCYNITLEQFCNQPLVLFREGYFLREAVSSYCQQHGINPDIRFETNLIELLKSLIRKKIGISTC
LPIILTEDSELVTVPFNPPIPIHLGIGWKASHYLSQASQALVTFLQEEMDGGQG
>Mature_294_residues
MDFKRLYYFTVLAQTGNFTKAAEELGIAQSALSMSIQKLEKQLALKLINRAERKMSITSDGQVLLKHAQKILDNVKQAKI
QLQELKGLESGTINLGTPAMIGSYYLPDTLALFKQRYPGIQINIHEAGTATLANMLLDGELDLAMIRADRHHDNIRCITL
AKEQIVACVPREHEFAKCYNITLEQFCNQPLVLFREGYFLREAVSSYCQQHGINPDIRFETNLIELLKSLIRKKIGISTC
LPIILTEDSELVTVPFNPPIPIHLGIGWKASHYLSQASQALVTFLQEEMDGGQG

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=258, Percent_Identity=29.0697674418605, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=25.3378378378378, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787879, Length=241, Percent_Identity=26.9709543568465, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=25, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=26.7796610169492, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693105, Length=280, Percent_Identity=24.2857142857143, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=22.6190476190476, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI2367136, Length=240, Percent_Identity=25.8333333333333, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=34.6938775510204, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=23.8255033557047, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=32.1167883211679, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789506, Length=294, Percent_Identity=22.4489795918367, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787806, Length=217, Percent_Identity=26.2672811059908, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788296, Length=241, Percent_Identity=23.6514522821577, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786401, Length=151, Percent_Identity=31.1258278145695, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082132, Length=305, Percent_Identity=23.6065573770492, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081904, Length=237, Percent_Identity=23.2067510548523, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789639, Length=171, Percent_Identity=25.1461988304094, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788706, Length=220, Percent_Identity=27.7272727272727, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787589, Length=148, Percent_Identity=32.4324324324324, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790262, Length=250, Percent_Identity=22.8, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=124, Percent_Identity=26.6129032258064, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786448, Length=199, Percent_Identity=28.643216080402, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789173, Length=119, Percent_Identity=33.6134453781513, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789204, Length=297, Percent_Identity=21.5488215488215, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33002; Mature: 33002

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKRLYYFTVLAQTGNFTKAAEELGIAQSALSMSIQKLEKQLALKLINRAERKMSITSD
CCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GQVLLKHAQKILDNVKQAKIQLQELKGLESGTINLGTPAMIGSYYLPDTLALFKQRYPGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCCCCCHHHHHHHHHHCCCE
QINIHEAGTATLANMLLDGELDLAMIRADRHHDNIRCITLAKEQIVACVPREHEFAKCYN
EEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEHHHHHHHCCCCCHHHHHHH
ITLEQFCNQPLVLFREGYFLREAVSSYCQQHGINPDIRFETNLIELLKSLIRKKIGISTC
HHHHHHCCCCEEEHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCHHHC
LPIILTEDSELVTVPFNPPIPIHLGIGWKASHYLSQASQALVTFLQEEMDGGQG
CEEEEECCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDFKRLYYFTVLAQTGNFTKAAEELGIAQSALSMSIQKLEKQLALKLINRAERKMSITSD
CCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GQVLLKHAQKILDNVKQAKIQLQELKGLESGTINLGTPAMIGSYYLPDTLALFKQRYPGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCCCCCHHHHHHHHHHCCCE
QINIHEAGTATLANMLLDGELDLAMIRADRHHDNIRCITLAKEQIVACVPREHEFAKCYN
EEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEHHHHHHHCCCCCHHHHHHH
ITLEQFCNQPLVLFREGYFLREAVSSYCQQHGINPDIRFETNLIELLKSLIRKKIGISTC
HHHHHHCCCCEEEHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCHHHC
LPIILTEDSELVTVPFNPPIPIHLGIGWKASHYLSQASQALVTFLQEEMDGGQG
CEEEEECCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]