Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 119946331

GI number: 119946331

Start: 3313510

End: 3315894

Strand: Reverse

Name: dosC [H]

Synonym: Ping_2702

Alternate gene names: 119946331

Gene position: 3315894-3313510 (Counterclockwise)

Preceding gene: 119946336

Following gene: 119946330

Centisome position: 72.72

GC content: 34.55

Gene sequence:

>2385_bases
TTGCCTAATATTATAAGAGTTTTTGTTTGTTTATGTACCTTGCTGAGTCAGCAATTATTCGCTGACGAGCTAAAAAAGGT
AACACTGCAACCCTCCTGGTTCGAACAATTCCAATCTGCCGGTTATTATATTGCTCAAGAAAAGGGTTTTTATAATGACG
TCGGATTAGACGTTACTATCAAAGATTATCAGTTTGGGAGCGATGCTGTACAAAAAATCAATGATGGTGAAATTGATTTT
GCCGTCGGTTACGAGAGCATAATACTTGGTAAGGCCAACCATAAAAAAATTGTCATTTTATATGCCCTTTTTCAATCAAG
TCCGCTTGTCCTGCTCAGTACCAAAGCATCAAAAATCAATAGCATCAGTCAGTTTGTTGGTAAAAAAATAATGACCACAA
ACAATGATTTTGCTCAAGCCTCATTAAAAGCAATGCTCAATTCAAATTATGTAGATTTGGAGAATGTAAATCTACTGCCC
CATTCGCACAATATAAATGATTTGGTCAATAAAAAAACAGATCTGATCAGCGCTTATATATCAAAAGCACCCTACGAGTT
AGATAAAATGGGGGTTACATATAATATTTTTGACCCTAAAGATTATGGTTTTGATATGTACAGTGATTTTTTATATACCA
GTGAAGCATTGATTGCAAAAGATATCACCACGGTTAAGGCATTTAAAGCGGCTTCATTAAAAGGTTGGCAATACGCATTT
ACCCATATAACTGAAACGGCCGATTTAATCTTTGCTAAATATAATGAGCAAAATTTAACCAAAGAAGAGCTTGTGTTCGA
AGGAAAAAAGCTTAAAAAGTTAGCATTTTTTAACACCGATCAGATAGGTGAAATAGATAACAATAAATTAAAAAGAATTT
TAGAACTATACAAAGTATTAGGCGTGACAAAAGGAAATGTTGATTTTACACAGCTTGTTTTTGACGAAAAAAATCCCAGA
TTAATTTTAACCGCAGAAGAACGTACTTATTTGAATCAGAAAAAACAGATTACTATGTGTATTGACCCCAATTGGCTGCC
CTATGAAGGTTTTGATCAACAAGGCAAGCATATTGGTTTAAATAGAGAATTTATTGATATTTTTAGAAAGCAGCTACCGA
TTCCTATTGAAATAGTCCAAACCAGCTCCTGGAGTGAGTCTCTGCAATTTGCAGAAGAGAGAAAATGTGACCTGCTATCT
TTAGCTGCAAAAACTGAAGAGAGAACAAAATATCTCAATTTCACCTCTCCTTATTTAGTCACCCCGCGTGTTTTAGTCAC
TAAAACTCATATCCCATTTATAGATAATTTTACGAATCTGTCCAATAAAAAAATAGGCGTGCCAAAAGGTTATGAACAGG
AAGAAATCATTCGACAAGATTATCCAAATATCATCATAGTAGAAGTTAATACGATAAAAGACGGGCTTGAAAAAGTGGAA
AATGGCGAACTTTATGGACTGATAGGCGGGCTCGACACTGTTGGTCACCTATTACAGGGTCGTTTTATTGGGCAACTCAA
AGTCAGTGGAAAACTTGATGAAAAAATGGAAGTTGGCATTGGGGTCAGAAATGATGACCTATTACTTTTGCAAGTCTCTG
AAAAACTAGTCAATAATTTATCAGAAGAAATAAAACAAAGTATCATTAATAACAGCAGTGCGATTAAATATAAAGAAGAG
TTTAATTATAAATTATTATGGCGGGTGCTGTTTGTTGTCTTGCTGTTGATTGCTTTTTTCTCATATCGGCAATGGCTGCT
AACAAAGTTAAATAAGACGCTAAATAAAAAAATAAATCAGAAAACTGCAGCATTACAGGAGCTAAATGAAAGCCTTGAAA
GAAGAATAAAGGAGAGGACCCAAAAGATAGAACATTCAAAAATGCTGTTGCAAGATGTCGCTTTTAAAGATAATTTAACC
AGTATTTTTAACCGGCATTACCTTTTTGAAATATCACCGATGCTTTTAACTGTATCTGAAGAGACGCAGACACCTTTATC
ACTGTTGTTAATTGATGTTGATCACTTCAAAAAAATCAATGATACCTATGGCCATTTGACTGGCGATAATATATTGAAAT
TTATTGTGAAAAATATTCAGGCAATATTAAGAGCAGATGATATTTTTGTGCGCTTTGGCGGGGAAGAGTTTATTATTTTA
CTGCCTAAAGCAAACCTTGAAGAAAGTGTGATGGTTGCAGAAAAACTACGACTCAGTGTGGAGCAAAATAATTACCACAA
AAACGGTATGGATATTTCTATCACCGTTAGTATTGGGGCCAGCCAGTACCAGCATACTGAGACCTTAGAAAAATTAATCA
GTCGGGCTGATTTGGCATTATATAGCGCAAAAGAAAAGGGGCGAAATCAAGTTAAAAAAAATTAA

Upstream 100 bases:

>100_bases
GTGTTTATAATGGTTGATTTTTCTTTAACTCATTTTACCTCGCAAAATAAACCGATTTGTCTATTCCTGCCAGCGCCTTA
ATATTTAAAAAGGGTTTTTT

Downstream 100 bases:

>100_bases
CTTAAATACAACGAATTAAAATTTTTCTGTTTCGCAATGTACTATATTTAAAATACGGGTTAAACAGAAAAGGATTTGAT
TTATGAAACAGCCACTACAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MPNIIRVFVCLCTLLSQQLFADELKKVTLQPSWFEQFQSAGYYIAQEKGFYNDVGLDVTIKDYQFGSDAVQKINDGEIDF
AVGYESIILGKANHKKIVILYALFQSSPLVLLSTKASKINSISQFVGKKIMTTNNDFAQASLKAMLNSNYVDLENVNLLP
HSHNINDLVNKKTDLISAYISKAPYELDKMGVTYNIFDPKDYGFDMYSDFLYTSEALIAKDITTVKAFKAASLKGWQYAF
THITETADLIFAKYNEQNLTKEELVFEGKKLKKLAFFNTDQIGEIDNNKLKRILELYKVLGVTKGNVDFTQLVFDEKNPR
LILTAEERTYLNQKKQITMCIDPNWLPYEGFDQQGKHIGLNREFIDIFRKQLPIPIEIVQTSSWSESLQFAEERKCDLLS
LAAKTEERTKYLNFTSPYLVTPRVLVTKTHIPFIDNFTNLSNKKIGVPKGYEQEEIIRQDYPNIIIVEVNTIKDGLEKVE
NGELYGLIGGLDTVGHLLQGRFIGQLKVSGKLDEKMEVGIGVRNDDLLLLQVSEKLVNNLSEEIKQSIINNSSAIKYKEE
FNYKLLWRVLFVVLLLIAFFSYRQWLLTKLNKTLNKKINQKTAALQELNESLERRIKERTQKIEHSKMLLQDVAFKDNLT
SIFNRHYLFEISPMLLTVSEETQTPLSLLLIDVDHFKKINDTYGHLTGDNILKFIVKNIQAILRADDIFVRFGGEEFIIL
LPKANLEESVMVAEKLRLSVEQNNYHKNGMDISITVSIGASQYQHTETLEKLISRADLALYSAKEKGRNQVKKN

Sequences:

>Translated_794_residues
MPNIIRVFVCLCTLLSQQLFADELKKVTLQPSWFEQFQSAGYYIAQEKGFYNDVGLDVTIKDYQFGSDAVQKINDGEIDF
AVGYESIILGKANHKKIVILYALFQSSPLVLLSTKASKINSISQFVGKKIMTTNNDFAQASLKAMLNSNYVDLENVNLLP
HSHNINDLVNKKTDLISAYISKAPYELDKMGVTYNIFDPKDYGFDMYSDFLYTSEALIAKDITTVKAFKAASLKGWQYAF
THITETADLIFAKYNEQNLTKEELVFEGKKLKKLAFFNTDQIGEIDNNKLKRILELYKVLGVTKGNVDFTQLVFDEKNPR
LILTAEERTYLNQKKQITMCIDPNWLPYEGFDQQGKHIGLNREFIDIFRKQLPIPIEIVQTSSWSESLQFAEERKCDLLS
LAAKTEERTKYLNFTSPYLVTPRVLVTKTHIPFIDNFTNLSNKKIGVPKGYEQEEIIRQDYPNIIIVEVNTIKDGLEKVE
NGELYGLIGGLDTVGHLLQGRFIGQLKVSGKLDEKMEVGIGVRNDDLLLLQVSEKLVNNLSEEIKQSIINNSSAIKYKEE
FNYKLLWRVLFVVLLLIAFFSYRQWLLTKLNKTLNKKINQKTAALQELNESLERRIKERTQKIEHSKMLLQDVAFKDNLT
SIFNRHYLFEISPMLLTVSEETQTPLSLLLIDVDHFKKINDTYGHLTGDNILKFIVKNIQAILRADDIFVRFGGEEFIIL
LPKANLEESVMVAEKLRLSVEQNNYHKNGMDISITVSIGASQYQHTETLEKLISRADLALYSAKEKGRNQVKKN
>Mature_793_residues
PNIIRVFVCLCTLLSQQLFADELKKVTLQPSWFEQFQSAGYYIAQEKGFYNDVGLDVTIKDYQFGSDAVQKINDGEIDFA
VGYESIILGKANHKKIVILYALFQSSPLVLLSTKASKINSISQFVGKKIMTTNNDFAQASLKAMLNSNYVDLENVNLLPH
SHNINDLVNKKTDLISAYISKAPYELDKMGVTYNIFDPKDYGFDMYSDFLYTSEALIAKDITTVKAFKAASLKGWQYAFT
HITETADLIFAKYNEQNLTKEELVFEGKKLKKLAFFNTDQIGEIDNNKLKRILELYKVLGVTKGNVDFTQLVFDEKNPRL
ILTAEERTYLNQKKQITMCIDPNWLPYEGFDQQGKHIGLNREFIDIFRKQLPIPIEIVQTSSWSESLQFAEERKCDLLSL
AAKTEERTKYLNFTSPYLVTPRVLVTKTHIPFIDNFTNLSNKKIGVPKGYEQEEIIRQDYPNIIIVEVNTIKDGLEKVEN
GELYGLIGGLDTVGHLLQGRFIGQLKVSGKLDEKMEVGIGVRNDDLLLLQVSEKLVNNLSEEIKQSIINNSSAIKYKEEF
NYKLLWRVLFVVLLLIAFFSYRQWLLTKLNKTLNKKINQKTAALQELNESLERRIKERTQKIEHSKMLLQDVAFKDNLTS
IFNRHYLFEISPMLLTVSEETQTPLSLLLIDVDHFKKINDTYGHLTGDNILKFIVKNIQAILRADDIFVRFGGEEFIILL
PKANLEESVMVAEKLRLSVEQNNYHKNGMDISITVSIGASQYQHTETLEKLISRADLALYSAKEKGRNQVKKN

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=43.9490445859873, Blast_Score=128, Evalue=2e-30,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=41.8181818181818, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=39.6341463414634, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=37.6543209876543, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787802, Length=172, Percent_Identity=37.2093023255814, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1787816, Length=126, Percent_Identity=38.8888888888889, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=32.183908045977, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=30.5555555555556, Blast_Score=93, Evalue=8e-20,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=35.8024691358025, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=30, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787056, Length=125, Percent_Identity=38.4, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=181, Percent_Identity=30.3867403314917, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 90996; Mature: 90865

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNIIRVFVCLCTLLSQQLFADELKKVTLQPSWFEQFQSAGYYIAQEKGFYNDVGLDVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEEEE
KDYQFGSDAVQKINDGEIDFAVGYESIILGKANHKKIVILYALFQSSPLVLLSTKASKIN
ECCCCCCHHHHHCCCCCEEEEECCCEEEEECCCCCEEEEEEEECCCCCEEEEECCHHHHH
SISQFVGKKIMTTNNDFAQASLKAMLNSNYVDLENVNLLPHSHNINDLVNKKTDLISAYI
HHHHHHCCCEECCCCHHHHHHHHHHHCCCCEEECCCEECCCCCCHHHHHHHHHHHHHHHH
SKAPYELDKMGVTYNIFDPKDYGFDMYSDFLYTSEALIAKDITTVKAFKAASLKGWQYAF
HHCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHH
THITETADLIFAKYNEQNLTKEELVFEGKKLKKLAFFNTDQIGEIDNNKLKRILELYKVL
HHHHHHHHEEEEECCCCCCCHHHHHHCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHH
GVTKGNVDFTQLVFDEKNPRLILTAEERTYLNQKKQITMCIDPNWLPYEGFDQQGKHIGL
CCCCCCCCEEEEEEECCCCEEEEEECHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEECC
NREFIDIFRKQLPIPIEIVQTSSWSESLQFAEERKCDLLSLAAKTEERTKYLNFTSPYLV
CHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCEEE
TPRVLVTKTHIPFIDNFTNLSNKKIGVPKGYEQEEIIRQDYPNIIIVEVNTIKDGLEKVE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHC
NGELYGLIGGLDTVGHLLQGRFIGQLKVSGKLDEKMEVGIGVRNDDLLLLQVSEKLVNNL
CCCEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCEEEEEEHHHHHHHH
SEEIKQSIINNSSAIKYKEEFNYKLLWRVLFVVLLLIAFFSYRQWLLTKLNKTLNKKINQ
HHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTAALQELNESLERRIKERTQKIEHSKMLLQDVAFKDNLTSIFNRHYLFEISPMLLTVSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEEEECCEEEEECC
ETQTPLSLLLIDVDHFKKINDTYGHLTGDNILKFIVKNIQAILRADDIFVRFGGEEFIIL
CCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEE
LPKANLEESVMVAEKLRLSVEQNNYHKNGMDISITVSIGASQYQHTETLEKLISRADLAL
ECCCCCCHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
YSAKEKGRNQVKKN
HHHHHHHHHHCCCC
>Mature Secondary Structure 
PNIIRVFVCLCTLLSQQLFADELKKVTLQPSWFEQFQSAGYYIAQEKGFYNDVGLDVTI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEEEE
KDYQFGSDAVQKINDGEIDFAVGYESIILGKANHKKIVILYALFQSSPLVLLSTKASKIN
ECCCCCCHHHHHCCCCCEEEEECCCEEEEECCCCCEEEEEEEECCCCCEEEEECCHHHHH
SISQFVGKKIMTTNNDFAQASLKAMLNSNYVDLENVNLLPHSHNINDLVNKKTDLISAYI
HHHHHHCCCEECCCCHHHHHHHHHHHCCCCEEECCCEECCCCCCHHHHHHHHHHHHHHHH
SKAPYELDKMGVTYNIFDPKDYGFDMYSDFLYTSEALIAKDITTVKAFKAASLKGWQYAF
HHCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHH
THITETADLIFAKYNEQNLTKEELVFEGKKLKKLAFFNTDQIGEIDNNKLKRILELYKVL
HHHHHHHHEEEEECCCCCCCHHHHHHCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHH
GVTKGNVDFTQLVFDEKNPRLILTAEERTYLNQKKQITMCIDPNWLPYEGFDQQGKHIGL
CCCCCCCCEEEEEEECCCCEEEEEECHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEECC
NREFIDIFRKQLPIPIEIVQTSSWSESLQFAEERKCDLLSLAAKTEERTKYLNFTSPYLV
CHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCEEE
TPRVLVTKTHIPFIDNFTNLSNKKIGVPKGYEQEEIIRQDYPNIIIVEVNTIKDGLEKVE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHC
NGELYGLIGGLDTVGHLLQGRFIGQLKVSGKLDEKMEVGIGVRNDDLLLLQVSEKLVNNL
CCCEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCEEEEEEHHHHHHHH
SEEIKQSIINNSSAIKYKEEFNYKLLWRVLFVVLLLIAFFSYRQWLLTKLNKTLNKKINQ
HHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTAALQELNESLERRIKERTQKIEHSKMLLQDVAFKDNLTSIFNRHYLFEISPMLLTVSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEEEECCEEEEECC
ETQTPLSLLLIDVDHFKKINDTYGHLTGDNILKFIVKNIQAILRADDIFVRFGGEEFIIL
CCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEE
LPKANLEESVMVAEKLRLSVEQNNYHKNGMDISITVSIGASQYQHTETLEKLISRADLAL
ECCCCCCHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
YSAKEKGRNQVKKN
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]