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The map label for this gene is pstA [H]

Identifier: 119946229

GI number: 119946229

Start: 3177676

End: 3178521

Strand: Direct

Name: pstA [H]

Synonym: Ping_2590

Alternate gene names: 119946229

Gene position: 3177676-3178521 (Clockwise)

Preceding gene: 119946228

Following gene: 119946230

Centisome position: 69.69

GC content: 41.13

Gene sequence:

>846_bases
ATGTTAACGCGAAAAACTCAAGATAAGTTTTTATTAGCAGCAACTTGGGCTGCCGCCACTGTTACGATCCTGTTTCTGCT
ATGGGTTGTTTGGCACGTTTTAAGTAATGGTTTAAGCTTTGTAACCTGGGAATTTATCACAAGTTCTTACACGCGAATTG
GCGAAGCATCAGGCATCTGGCCAATGATCGTATCAACCGTTTATATGGTTGTTCTGACGCTGTCTATTGCGACCCCTATT
GGTATAATGACGGCTATTTACTTAACTGAATATGCCAAACCGGGCAGTAAAATAGTACAAGTAATACGTTTTTGTATCGA
ATCGCTAGCAGGTATTCCCTCAATCGTTTACGGTCTATTTGGTATGACCTTTTTTGTTACCACATTAGGGCTGGGATTCT
CCATTTTAGCCGGTTCCTTAACCTTATCGATACTTATCTTACCGGTTATTATCCGTACCACCGAAGAGGCGCTAATGGCG
GTACCGATGAGTTATCGTGAAGGTGGTTATGCTTTAGGCAGCTCTAAAATATATACTATTTGGCGTTTAATTTTACCCAG
TGCCATGCCAGGTATTATCACTGCGATTATCCTAAGTATCGGCCGTGTTATTGGTGAATCTGCACCGGTATTCTTAACCG
CAGGTATGGTCACTAAAATTCCTGAATCGGTATTGGACTCAGGCAGAACATTAACAGTACACTTGTACAAATTAACCCAA
GAGCTCTTTAGCCAAAATGAATGGGATCAGGCTTATGCAACGGCAACCGTTCTGATTGTGTTAGTGCTTTTCTTAAATTT
AATCACGAAACTCATTGCTACCCGCTACGCTAAAAAATCTTACTAA

Upstream 100 bases:

>100_bases
TACATGCCAGCGCACTTTATGCGACCGGTATTGTTTTATTGGTATTTATCGTCATATTGAATGCAGTTATTTTATACATT
AACAAAAAGAGAGCCTACTA

Downstream 100 bases:

>100_bases
AGGCCATATAAAATGAACAAGCTTGAAATAAACAACCTGAATCTTTTCTATGGCAAAAACCATGCATTAAAAGATATTTC
ATTTATCATTCCCGAGCGCA

Product: ABC phosphate transporter inner membrane protein PstA

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLTRKTQDKFLLAATWAAATVTILFLLWVVWHVLSNGLSFVTWEFITSSYTRIGEASGIWPMIVSTVYMVVLTLSIATPI
GIMTAIYLTEYAKPGSKIVQVIRFCIESLAGIPSIVYGLFGMTFFVTTLGLGFSILAGSLTLSILILPVIIRTTEEALMA
VPMSYREGGYALGSSKIYTIWRLILPSAMPGIITAIILSIGRVIGESAPVFLTAGMVTKIPESVLDSGRTLTVHLYKLTQ
ELFSQNEWDQAYATATVLIVLVLFLNLITKLIATRYAKKSY

Sequences:

>Translated_281_residues
MLTRKTQDKFLLAATWAAATVTILFLLWVVWHVLSNGLSFVTWEFITSSYTRIGEASGIWPMIVSTVYMVVLTLSIATPI
GIMTAIYLTEYAKPGSKIVQVIRFCIESLAGIPSIVYGLFGMTFFVTTLGLGFSILAGSLTLSILILPVIIRTTEEALMA
VPMSYREGGYALGSSKIYTIWRLILPSAMPGIITAIILSIGRVIGESAPVFLTAGMVTKIPESVLDSGRTLTVHLYKLTQ
ELFSQNEWDQAYATATVLIVLVLFLNLITKLIATRYAKKSY
>Mature_281_residues
MLTRKTQDKFLLAATWAAATVTILFLLWVVWHVLSNGLSFVTWEFITSSYTRIGEASGIWPMIVSTVYMVVLTLSIATPI
GIMTAIYLTEYAKPGSKIVQVIRFCIESLAGIPSIVYGLFGMTFFVTTLGLGFSILAGSLTLSILILPVIIRTTEEALMA
VPMSYREGGYALGSSKIYTIWRLILPSAMPGIITAIILSIGRVIGESAPVFLTAGMVTKIPESVLDSGRTLTVHLYKLTQ
ELFSQNEWDQAYATATVLIVLVLFLNLITKLIATRYAKKSY

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=280, Percent_Identity=31.0714285714286, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790164, Length=235, Percent_Identity=27.6595744680851, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30854; Mature: 30854

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKTQDKFLLAATWAAATVTILFLLWVVWHVLSNGLSFVTWEFITSSYTRIGEASGIW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
PMIVSTVYMVVLTLSIATPIGIMTAIYLTEYAKPGSKIVQVIRFCIESLAGIPSIVYGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
GMTFFVTTLGLGFSILAGSLTLSILILPVIIRTTEEALMAVPMSYREGGYALGSSKIYTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEECCCHHHHHH
WRLILPSAMPGIITAIILSIGRVIGESAPVFLTAGMVTKIPESVLDSGRTLTVHLYKLTQ
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHCCHHHHCCCCEEEHHHHHHHH
ELFSQNEWDQAYATATVLIVLVLFLNLITKLIATRYAKKSY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTRKTQDKFLLAATWAAATVTILFLLWVVWHVLSNGLSFVTWEFITSSYTRIGEASGIW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
PMIVSTVYMVVLTLSIATPIGIMTAIYLTEYAKPGSKIVQVIRFCIESLAGIPSIVYGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
GMTFFVTTLGLGFSILAGSLTLSILILPVIIRTTEEALMAVPMSYREGGYALGSSKIYTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEECCCHHHHHH
WRLILPSAMPGIITAIILSIGRVIGESAPVFLTAGMVTKIPESVLDSGRTLTVHLYKLTQ
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHCCHHHHCCCCEEEHHHHHHHH
ELFSQNEWDQAYATATVLIVLVLFLNLITKLIATRYAKKSY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]