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The map label for this gene is norM [H]

Identifier: 119946004

GI number: 119946004

Start: 2854807

End: 2856189

Strand: Direct

Name: norM [H]

Synonym: Ping_2346

Alternate gene names: 119946004

Gene position: 2854807-2856189 (Clockwise)

Preceding gene: 119945997

Following gene: 119946008

Centisome position: 62.61

GC content: 42.88

Gene sequence:

>1383_bases
GTGCCTTACTCCCGAGAAATAAGAAAAATACTCAAGCTAAGTTTCCCTATTTTTATCGCACAATTTGCACAAACGGCCAT
GGGTTTTGTAGATACTGTGATGGCCGGTGGTGTTAGCGCAAATGACATGGCTGCAGTAGCTATTGCCACCAGCATTTGGT
TACCCTGTATTTTGTTTGGCGTCGGTGTGTTAATGGCACTTATTCCCCTCACGGCGCAAGCCCATGGTGCCAACGATATA
GAAAATATTCCCAGTATTGGCCAACAGGGGTTGTATTTGGCTTTATTACTCAGCATCCCCACCTTCTTGATTTTATATAA
TGCCAGTTACCTTATCAATATCATGGATATGGATCCCATTTTAAAAGATATTACTACTCGCTATCTGTACGCTATTTTAT
CCGCCATACCGGCTTTTTTATTATTCCAGGCACTGCGTAACTATATAGAAGGTCTGTCACTGACGAAACCTGCAATGGTG
ATAGGTTTTATGGGATTAATCATTAATATTCCACTGAACTGGATTTTTGTCTATGGGAAATTAGGGCTTCCGGCAATGGG
TGGTGCAGGATGTGGCGTCGCAACTAGCCTCGTTTATTGGCTAATGCTGTCGGCGCTCACGGGTTATGTATGGCATACTA
AAAAACTTCAGTATACCCCTTTATTTAAACGCTTCTCTGCCATTAACTTGAGAACACAATTAGCCATCTTTAGCCTCGGT
TTACCCGTTGCAATCGCATTATTTTTTGAGATCTCTATTTTTGCGATCGTCGCGCTATTAATCGCACCATTAGGCGCAAA
CATAGTGGCGGCCCATCAAATTGCGATCAATTTTTCCAGCATGGTCTTTATGCTCCCCATGAGCATCGCCAATGCGGTCA
GTATTAGGGTTGGTTTTAACTTAGGACAAAAAAATAAAGCAGAGGCCAAAAATGCCAGTTATGCTGGGTTTATTGTCGGT
TTATCGCTCTCAGTGATAACCGCATTATTAACCTTGATATTCAAGGAGCAGATCGCACAGCTTTACACGGATGACCCGAA
GGTGATTTTATTAGCCGTTACCCTAATAATATTTTCTGCAATGTACCAGTGTGTTGATGCCATTCAGGCGGTAGCAGCTG
GTGCCCTGCGTGGTTACAAAGAGATGAAGGCGATATTTTTACGTACCTTTATTGCGTATTGGATGATTGGATTACCCCTT
GGTTATAGCCTGGCAATGACGGATTTATTTATGCCCGCACTTGGCGCAAAAGGGTTTTGGATTGGTATTACGGCAGGTTT
AACGTCTGCCGCTATATTATTAGGAAGGCGTCTCTGGGTCGTGCAAAAGTGCTCAGCAGAACTGTGCCAAGCAGATAAAT
CTGTTGCGAATAATTGTTATTAA

Upstream 100 bases:

>100_bases
ACTTATCCTATAATTTTTGCACTCGCTAAGATTGCAATAGCCCCCCCTAACGCGTATAAATCAACTGTAAATTTTATCCT
TTATTAAATTGGAAAGTTTT

Downstream 100 bases:

>100_bases
GCTTATTACTTATACCAAACACATTGAAATTTCAAGAATTAAAAAGACAGCAGCCGCTAAATATAACAGAGCAAAAAGGC
AACCGTTCGATTATTTAACA

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MPYSREIRKILKLSFPIFIAQFAQTAMGFVDTVMAGGVSANDMAAVAIATSIWLPCILFGVGVLMALIPLTAQAHGANDI
ENIPSIGQQGLYLALLLSIPTFLILYNASYLINIMDMDPILKDITTRYLYAILSAIPAFLLFQALRNYIEGLSLTKPAMV
IGFMGLIINIPLNWIFVYGKLGLPAMGGAGCGVATSLVYWLMLSALTGYVWHTKKLQYTPLFKRFSAINLRTQLAIFSLG
LPVAIALFFEISIFAIVALLIAPLGANIVAAHQIAINFSSMVFMLPMSIANAVSIRVGFNLGQKNKAEAKNASYAGFIVG
LSLSVITALLTLIFKEQIAQLYTDDPKVILLAVTLIIFSAMYQCVDAIQAVAAGALRGYKEMKAIFLRTFIAYWMIGLPL
GYSLAMTDLFMPALGAKGFWIGITAGLTSAAILLGRRLWVVQKCSAELCQADKSVANNCY

Sequences:

>Translated_460_residues
MPYSREIRKILKLSFPIFIAQFAQTAMGFVDTVMAGGVSANDMAAVAIATSIWLPCILFGVGVLMALIPLTAQAHGANDI
ENIPSIGQQGLYLALLLSIPTFLILYNASYLINIMDMDPILKDITTRYLYAILSAIPAFLLFQALRNYIEGLSLTKPAMV
IGFMGLIINIPLNWIFVYGKLGLPAMGGAGCGVATSLVYWLMLSALTGYVWHTKKLQYTPLFKRFSAINLRTQLAIFSLG
LPVAIALFFEISIFAIVALLIAPLGANIVAAHQIAINFSSMVFMLPMSIANAVSIRVGFNLGQKNKAEAKNASYAGFIVG
LSLSVITALLTLIFKEQIAQLYTDDPKVILLAVTLIIFSAMYQCVDAIQAVAAGALRGYKEMKAIFLRTFIAYWMIGLPL
GYSLAMTDLFMPALGAKGFWIGITAGLTSAAILLGRRLWVVQKCSAELCQADKSVANNCY
>Mature_459_residues
PYSREIRKILKLSFPIFIAQFAQTAMGFVDTVMAGGVSANDMAAVAIATSIWLPCILFGVGVLMALIPLTAQAHGANDIE
NIPSIGQQGLYLALLLSIPTFLILYNASYLINIMDMDPILKDITTRYLYAILSAIPAFLLFQALRNYIEGLSLTKPAMVI
GFMGLIINIPLNWIFVYGKLGLPAMGGAGCGVATSLVYWLMLSALTGYVWHTKKLQYTPLFKRFSAINLRTQLAIFSLGL
PVAIALFFEISIFAIVALLIAPLGANIVAAHQIAINFSSMVFMLPMSIANAVSIRVGFNLGQKNKAEAKNASYAGFIVGL
SLSVITALLTLIFKEQIAQLYTDDPKVILLAVTLIIFSAMYQCVDAIQAVAAGALRGYKEMKAIFLRTFIAYWMIGLPLG
YSLAMTDLFMPALGAKGFWIGITAGLTSAAILLGRRLWVVQKCSAELCQADKSVANNCY

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=457, Percent_Identity=24.2888402625821, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI153792564, Length=427, Percent_Identity=24.824355971897, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI38261960, Length=463, Percent_Identity=23.5421166306695, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI48994890, Length=442, Percent_Identity=50.4524886877828, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI226510954, Length=465, Percent_Identity=21.7204301075269, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320545, Length=438, Percent_Identity=23.7442922374429, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321821, Length=329, Percent_Identity=23.1003039513678, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49867; Mature: 49736

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYSREIRKILKLSFPIFIAQFAQTAMGFVDTVMAGGVSANDMAAVAIATSIWLPCILFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VGVLMALIPLTAQAHGANDIENIPSIGQQGLYLALLLSIPTFLILYNASYLINIMDMDPI
HHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEECCCCHH
LKDITTRYLYAILSAIPAFLLFQALRNYIEGLSLTKPAMVIGFMGLIINIPLNWIFVYGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHEEEEEEC
LGLPAMGGAGCGVATSLVYWLMLSALTGYVWHTKKLQYTPLFKRFSAINLRTQLAIFSLG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPVAIALFFEISIFAIVALLIAPLGANIVAAHQIAINFSSMVFMLPMSIANAVSIRVGFN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
LGQKNKAEAKNASYAGFIVGLSLSVITALLTLIFKEQIAQLYTDDPKVILLAVTLIIFSA
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MYQCVDAIQAVAAGALRGYKEMKAIFLRTFIAYWMIGLPLGYSLAMTDLFMPALGAKGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEE
IGITAGLTSAAILLGRRLWVVQKCSAELCQADKSVANNCY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PYSREIRKILKLSFPIFIAQFAQTAMGFVDTVMAGGVSANDMAAVAIATSIWLPCILFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VGVLMALIPLTAQAHGANDIENIPSIGQQGLYLALLLSIPTFLILYNASYLINIMDMDPI
HHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEECCCCHH
LKDITTRYLYAILSAIPAFLLFQALRNYIEGLSLTKPAMVIGFMGLIINIPLNWIFVYGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHEEEEEEC
LGLPAMGGAGCGVATSLVYWLMLSALTGYVWHTKKLQYTPLFKRFSAINLRTQLAIFSLG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPVAIALFFEISIFAIVALLIAPLGANIVAAHQIAINFSSMVFMLPMSIANAVSIRVGFN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
LGQKNKAEAKNASYAGFIVGLSLSVITALLTLIFKEQIAQLYTDDPKVILLAVTLIIFSA
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MYQCVDAIQAVAAGALRGYKEMKAIFLRTFIAYWMIGLPLGYSLAMTDLFMPALGAKGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEE
IGITAGLTSAAILLGRRLWVVQKCSAELCQADKSVANNCY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA