Click here to switch to the map view.

The map label for this gene is rlmG

Identifier: 119945956

GI number: 119945956

Start: 2790187

End: 2791335

Strand: Direct

Name: rlmG

Synonym: Ping_2296

Alternate gene names: 119945956

Gene position: 2790187-2791335 (Clockwise)

Preceding gene: 119945954

Following gene: 119945963

Centisome position: 61.19

GC content: 37.6

Gene sequence:

>1149_bases
ATGAATGAAGCACTGAACATTGCAGACCAAACTTTGGTTCTGCATCGTCACCCAAGAATAAAAAATGAAACATTACAAGC
ATGGGATTCTGCTGATGAATACCTCATCAATTACTTCTCTGAAAATGAACTGCAAAAACAGCTAAATGAAAATGAGCAAA
TATTGCTTGTTAACGACAGCTTCGGCGCACTGACTTGTTACCTGCAAGCATTTCAGCGTACTGTTGTTTCAGACTCATAC
TTATCCATTAATTCAATCAAGCTAAATATGCAGCGTAATCGTTGCCCGGAAGAAAATCTTCTTTTACAGGACAGTCTGCA
GCAGTTTCCTGAAAACGTAAAAATTGTCTTGATTAAAATACCTAAAACACTTTCTTACCTTGAAGAAATGTTACAAAAAT
TACATGCAGTTATAACGCCTGAAACTATCGTTGTGGCGGCCGGTAAAGTCAATATGATTCATAACTCTACTCTAGATTTA
TTTGAAAAATATATCGGCACAACCAAAACATCATTGGCGAAAAAGAAATCACGTTTAATTTTCAGCTTACCAATAATTAA
AGAAGTACCCGAAAAAGAGATAGCAATTACCTGGGAAATTGAAAAGCTTGATTGGAAAATCCATAACCATGCCAATGTAT
TTTCTCGTAACCACTTAGATATTGGCGGTCGTTTCTTAATGGATAATCTGCCTAAAGGCGATTTTTCTAAGGTTGTAGAC
TTAGGTTGTGGTAACGGAATTATTGGTATGGCGGCGTCTGCCGCATATCCGAAAGCACAAATCACCTTTATTGATGAGTC
CTACATGTCGATTGATTCTGCCCGTATTAACATGCAGAAAAATTTACCTGAAGAACAAGCTGAAAATGCGCGATTTGTGG
TTAATAACGGATTAGTGGGTTTCAAACCCCGCAGCTATGATCTGATTTTATGTAACCCGCCTTTTCATCAGCAACAAACA
ATCACCGATCAGATTGCCTGGAGCATGTTTAATGATGCCCATTTCTGTTTAGTCGATAATGGTGAATTAGTGATAGTGGG
TAACCACCATCTTAACTATCAGGACAAACTCGAGCGTATCTTTGGCAACTGTGAAATCGTTAGCCAGAACAAAAAGTTTG
TTATTTTACGTGCAGTAAAAATGGATTAG

Upstream 100 bases:

>100_bases
TAATTATGCCGCAAATGACAATATTACTTACCTAATGGGCTGGTTCACGTTAAAATGGATTTTTTATATTGACACTATTT
TAAACACTGAGCAAAATCCT

Downstream 100 bases:

>100_bases
TTTTCAAAAAAATGTGCACAATAAAAAAGCAGCTCAATGAGCTGCTTTTTTTTGTTAAAAAAAACCGTTTGGTTTTACCC
AAACGGTTTTTATCTATTGC

Product: rRNA (guanine-N(2)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNEALNIADQTLVLHRHPRIKNETLQAWDSADEYLINYFSENELQKQLNENEQILLVNDSFGALTCYLQAFQRTVVSDSY
LSINSIKLNMQRNRCPEENLLLQDSLQQFPENVKIVLIKIPKTLSYLEEMLQKLHAVITPETIVVAAGKVNMIHNSTLDL
FEKYIGTTKTSLAKKKSRLIFSLPIIKEVPEKEIAITWEIEKLDWKIHNHANVFSRNHLDIGGRFLMDNLPKGDFSKVVD
LGCGNGIIGMAASAAYPKAQITFIDESYMSIDSARINMQKNLPEEQAENARFVVNNGLVGFKPRSYDLILCNPPFHQQQT
ITDQIAWSMFNDAHFCLVDNGELVIVGNHHLNYQDKLERIFGNCEIVSQNKKFVILRAVKMD

Sequences:

>Translated_382_residues
MNEALNIADQTLVLHRHPRIKNETLQAWDSADEYLINYFSENELQKQLNENEQILLVNDSFGALTCYLQAFQRTVVSDSY
LSINSIKLNMQRNRCPEENLLLQDSLQQFPENVKIVLIKIPKTLSYLEEMLQKLHAVITPETIVVAAGKVNMIHNSTLDL
FEKYIGTTKTSLAKKKSRLIFSLPIIKEVPEKEIAITWEIEKLDWKIHNHANVFSRNHLDIGGRFLMDNLPKGDFSKVVD
LGCGNGIIGMAASAAYPKAQITFIDESYMSIDSARINMQKNLPEEQAENARFVVNNGLVGFKPRSYDLILCNPPFHQQQT
ITDQIAWSMFNDAHFCLVDNGELVIVGNHHLNYQDKLERIFGNCEIVSQNKKFVILRAVKMD
>Mature_382_residues
MNEALNIADQTLVLHRHPRIKNETLQAWDSADEYLINYFSENELQKQLNENEQILLVNDSFGALTCYLQAFQRTVVSDSY
LSINSIKLNMQRNRCPEENLLLQDSLQQFPENVKIVLIKIPKTLSYLEEMLQKLHAVITPETIVVAAGKVNMIHNSTLDL
FEKYIGTTKTSLAKKKSRLIFSLPIIKEVPEKEIAITWEIEKLDWKIHNHANVFSRNHLDIGGRFLMDNLPKGDFSKVVD
LGCGNGIIGMAASAAYPKAQITFIDESYMSIDSARINMQKNLPEEQAENARFVVNNGLVGFKPRSYDLILCNPPFHQQQT
ITDQIAWSMFNDAHFCLVDNGELVIVGNHHLNYQDKLERIFGNCEIVSQNKKFVILRAVKMD

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=376, Percent_Identity=44.4148936170213, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1790830, Length=168, Percent_Identity=32.7380952380952, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_PSYIN (A1SX22)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943636.1
- ProteinModelPortal:   A1SX22
- STRING:   A1SX22
- GeneID:   4624614
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2296
- eggNOG:   COG2813
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- PhylomeDB:   A1SX22
- BioCyc:   PING357804:PING_2296-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 43653; Mature: 43653

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEALNIADQTLVLHRHPRIKNETLQAWDSADEYLINYFSENELQKQLNENEQILLVNDS
CCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
FGALTCYLQAFQRTVVSDSYLSINSIKLNMQRNRCPEENLLLQDSLQQFPENVKIVLIKI
CCHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHCCCCCCCCHHHHHHHHCCCCEEEEEEEC
PKTLSYLEEMLQKLHAVITPETIVVAAGKVNMIHNSTLDLFEKYIGTTKTSLAKKKSRLI
CHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHHHHHCCCHHHHHHHHCCEE
FSLPIIKEVPEKEIAITWEIEKLDWKIHNHANVFSRNHLDIGGRFLMDNLPKGDFSKVVD
EEECHHHHCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHC
LGCGNGIIGMAASAAYPKAQITFIDESYMSIDSARINMQKNLPEEQAENARFVVNNGLVG
CCCCCCHHHHCCCCCCCCEEEEEEEHHHHHCCHHEEEEHHCCCHHHHCCCEEEEECCEEE
FKPRSYDLILCNPPFHQQQTITDQIAWSMFNDAHFCLVDNGELVIVGNHHLNYQDKLERI
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCCHHHHHHHH
FGNCEIVSQNKKFVILRAVKMD
HCCCEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MNEALNIADQTLVLHRHPRIKNETLQAWDSADEYLINYFSENELQKQLNENEQILLVNDS
CCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
FGALTCYLQAFQRTVVSDSYLSINSIKLNMQRNRCPEENLLLQDSLQQFPENVKIVLIKI
CCHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHCCCCCCCCHHHHHHHHCCCCEEEEEEEC
PKTLSYLEEMLQKLHAVITPETIVVAAGKVNMIHNSTLDLFEKYIGTTKTSLAKKKSRLI
CHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHHHHHCCCHHHHHHHHCCEE
FSLPIIKEVPEKEIAITWEIEKLDWKIHNHANVFSRNHLDIGGRFLMDNLPKGDFSKVVD
EEECHHHHCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHC
LGCGNGIIGMAASAAYPKAQITFIDESYMSIDSARINMQKNLPEEQAENARFVVNNGLVG
CCCCCCHHHHCCCCCCCCEEEEEEEHHHHHCCHHEEEEHHCCCHHHHCCCEEEEECCEEE
FKPRSYDLILCNPPFHQQQTITDQIAWSMFNDAHFCLVDNGELVIVGNHHLNYQDKLERI
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCCHHHHHHHH
FGNCEIVSQNKKFVILRAVKMD
HCCCEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA