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The map label for this gene is rlmL

Identifier: 119945943

GI number: 119945943

Start: 2772555

End: 2774804

Strand: Reverse

Name: rlmL

Synonym: Ping_2283

Alternate gene names: 119945943

Gene position: 2774804-2772555 (Counterclockwise)

Preceding gene: 119945944

Following gene: 119945942

Centisome position: 60.86

GC content: 40.67

Gene sequence:

>2250_bases
ATGTTAAGTTATTTTATCCCTACCGCTAAAGGGTTGGCCCCTCTGCTTGAAGTTGAGCTAAAAGAGATGGGTATTGAAAA
TCCGCAACAAATGAATGGTGGCGTGAAATTCGAGGGCACCTTAGAGCAAGGGTATAAAGTTTGTTTATGGTCTCGTTTTG
CCAGCCGAGTATTGTTAAAGCTTTCTGAATTCAAAGTGTTAGATTCAATGGATCTGTATTTAGGCTGCAGCAATATTCCC
TGGGAAACGCATTTTGATGTTGATAAAACCTTCTCTATCGATTTTTCCGGTACCAACGATGAAATTCGTAATACTCAGTT
TGGTGCGTTAAAAATTAAAGATGCGATTGTGGATCGTTTCCGTAAGCACTTTGATGAGCGTCCAAATGTTCAAAAACGTG
ATGCTGATATTCGTTTTAACGGCCGTTTATGGAAAGACAAGGCGACTATTTATTTAGACCTGTCGGGTTCGCCGCTGCAC
CTTCGTGGTTACCGTACTATCGCAGGCGAAGCGCCACTGCGTGAGACCTTAGCTGCTGGTATTATTAAACGCAGTGGTTG
GCAGGGTGAAGCTCTTTTAGACCCAATGTGTGGCTCAGGTACTTTGGTTATAGAAGCGGCCATGATGGCGTTAAATATAG
CGCCAGGCTCGTTGCGTGAAACCTTTGGTTTTGAAAAGTGGAAAAAACACGATCAGGAATGCTGGCAAACGCTTAAAACA
TCGGCACAAGTTTATGGACGTCGCGCAGTCAATCAGTGTGAAACACGTTTTTACGGATCTGATTTAAGCAAAGATATGAT
TGAAATTGCCCGCAAAAATGCACAACGGGCAGGGGTTGGCGAAGTAATTGAATTTTCTGTGACCGATGCTAAAAAAGTAC
TACCACCCGAAGAACTTGAAACCGGAATGTTAATTACTAACCCTCCCTATGGTGAACGTCTAGGCAGTTTCAGCGATACC
ATCACACTTTATACTGAGCTTGGTTATCATTTTAAAGATGCCTTTGCCGGTTGGAATTTATCTATGTTTGCCATGGACAC
TGAATTATTAAGTTGTTTAGGGATGCGTGCGGGTAAAAGTTTTAAATTCTTTAATGGACCGATTGAGTGTGTGCTTAAGA
ATTACCGTATTTCGCCAAAACGTCCGGCCGTAGAAGTTGAACCGGTAGCCCCGAAAAAGACAAACAAAGAAACCCCTGAG
CCTATCAATCCCTGGACCATGGGTCAAAATGAAAGTGTTGTTGAAGCCGCAATCGAAAATGAGTCTGAGCTTGTTTTTAA
AGAGGTTCAACAAATAAAACCCGCTATTTATGCGGAAGAATTTGCTAACCGTTTACTTAAAAACCTAAAACAACTGGAAA
AATGGGCAAAACGTGAAGGGGTTGAATGTTACCGTTTATATGATGCCGATTTACCTGAATATAACGTCGCTATTGACCGT
TATGCTGAATATATTATTATTCAAGAATACCGTGCTCCCAAAGAGATTGAGACACAAAAAGTACGTCGCCGCTTCTTAGA
TGTTGTTTCTACAGTACGCTACGTATTAAATCTCCCCGATGACAAATTAGTTATTAAAGTACGTGAACGTCAGAAAGGGC
GTCAGCAATATGAAAAATTGGATACCAAAAAACAGTCATTAGTCGTCCATGAAGGGCAGGCCAAAATGCTGGTTAATTTG
CAGGATTATTTGGATACTGGCCTGTTTTTAGATCATAGACCAACCCGTTTACTGATTGGCAACATGGCTAAAGGCAAAGA
TTTTTTAAATCTATTTTGTTATACGGCAACGGCTTCTGTGCATGCTGCACTGGGTGGTGCTAAATCAACAACCAGTGTTG
ATATGTCTAAGACTTATCTCGCCTGGGGCGAAGATAACTTTGCAGAAAACGGCATTAAAGGTAAACATGAGTTTATTCAG
CAGGACTGTATTAAATGGCTACAGCATGCCCACGAGATGTATGACCTTATTTTTATTGATCCGCCAACCTTCTCAAATTC
CAAACGTATGAATGATGTGTTTGACGTGCAGGAGGATCATGTTGCACTGTTAACCTCTGCCAGTCAGCGTTTAAATAAGC
AGGGCGAAATTATTTTCTCAAATAATAAACGTGGTTTTAAATTAGATGTTGATGCTATTAAAGCATTAGGTTTTTATATT
AAAGATATCAGCCAGTCCTCCATACCGGAAGACTTTAGACGCAATAAAAAAATTCATCAGTGCTGGATTTTAACTAAGCA
TACTGATTAA

Upstream 100 bases:

>100_bases
CGACAGCTAACAACCGCTCGATATTAATTATGTTGGGATAATCGAATTTTAAGGGTATAATACGTCCCATTTTATTTTCT
ATAATCCCGTGAGATCCTAT

Downstream 100 bases:

>100_bases
GAGAAATAATAGTGCAGAAGCATTTTTTATTATATTTTACGGACGGCTGCCATTTATGTGATGATGCACAGAGATTATTG
CAGCAGACGTCTGTTTCATA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MLSYFIPTAKGLAPLLEVELKEMGIENPQQMNGGVKFEGTLEQGYKVCLWSRFASRVLLKLSEFKVLDSMDLYLGCSNIP
WETHFDVDKTFSIDFSGTNDEIRNTQFGALKIKDAIVDRFRKHFDERPNVQKRDADIRFNGRLWKDKATIYLDLSGSPLH
LRGYRTIAGEAPLRETLAAGIIKRSGWQGEALLDPMCGSGTLVIEAAMMALNIAPGSLRETFGFEKWKKHDQECWQTLKT
SAQVYGRRAVNQCETRFYGSDLSKDMIEIARKNAQRAGVGEVIEFSVTDAKKVLPPEELETGMLITNPPYGERLGSFSDT
ITLYTELGYHFKDAFAGWNLSMFAMDTELLSCLGMRAGKSFKFFNGPIECVLKNYRISPKRPAVEVEPVAPKKTNKETPE
PINPWTMGQNESVVEAAIENESELVFKEVQQIKPAIYAEEFANRLLKNLKQLEKWAKREGVECYRLYDADLPEYNVAIDR
YAEYIIIQEYRAPKEIETQKVRRRFLDVVSTVRYVLNLPDDKLVIKVRERQKGRQQYEKLDTKKQSLVVHEGQAKMLVNL
QDYLDTGLFLDHRPTRLLIGNMAKGKDFLNLFCYTATASVHAALGGAKSTTSVDMSKTYLAWGEDNFAENGIKGKHEFIQ
QDCIKWLQHAHEMYDLIFIDPPTFSNSKRMNDVFDVQEDHVALLTSASQRLNKQGEIIFSNNKRGFKLDVDAIKALGFYI
KDISQSSIPEDFRRNKKIHQCWILTKHTD

Sequences:

>Translated_749_residues
MLSYFIPTAKGLAPLLEVELKEMGIENPQQMNGGVKFEGTLEQGYKVCLWSRFASRVLLKLSEFKVLDSMDLYLGCSNIP
WETHFDVDKTFSIDFSGTNDEIRNTQFGALKIKDAIVDRFRKHFDERPNVQKRDADIRFNGRLWKDKATIYLDLSGSPLH
LRGYRTIAGEAPLRETLAAGIIKRSGWQGEALLDPMCGSGTLVIEAAMMALNIAPGSLRETFGFEKWKKHDQECWQTLKT
SAQVYGRRAVNQCETRFYGSDLSKDMIEIARKNAQRAGVGEVIEFSVTDAKKVLPPEELETGMLITNPPYGERLGSFSDT
ITLYTELGYHFKDAFAGWNLSMFAMDTELLSCLGMRAGKSFKFFNGPIECVLKNYRISPKRPAVEVEPVAPKKTNKETPE
PINPWTMGQNESVVEAAIENESELVFKEVQQIKPAIYAEEFANRLLKNLKQLEKWAKREGVECYRLYDADLPEYNVAIDR
YAEYIIIQEYRAPKEIETQKVRRRFLDVVSTVRYVLNLPDDKLVIKVRERQKGRQQYEKLDTKKQSLVVHEGQAKMLVNL
QDYLDTGLFLDHRPTRLLIGNMAKGKDFLNLFCYTATASVHAALGGAKSTTSVDMSKTYLAWGEDNFAENGIKGKHEFIQ
QDCIKWLQHAHEMYDLIFIDPPTFSNSKRMNDVFDVQEDHVALLTSASQRLNKQGEIIFSNNKRGFKLDVDAIKALGFYI
KDISQSSIPEDFRRNKKIHQCWILTKHTD
>Mature_749_residues
MLSYFIPTAKGLAPLLEVELKEMGIENPQQMNGGVKFEGTLEQGYKVCLWSRFASRVLLKLSEFKVLDSMDLYLGCSNIP
WETHFDVDKTFSIDFSGTNDEIRNTQFGALKIKDAIVDRFRKHFDERPNVQKRDADIRFNGRLWKDKATIYLDLSGSPLH
LRGYRTIAGEAPLRETLAAGIIKRSGWQGEALLDPMCGSGTLVIEAAMMALNIAPGSLRETFGFEKWKKHDQECWQTLKT
SAQVYGRRAVNQCETRFYGSDLSKDMIEIARKNAQRAGVGEVIEFSVTDAKKVLPPEELETGMLITNPPYGERLGSFSDT
ITLYTELGYHFKDAFAGWNLSMFAMDTELLSCLGMRAGKSFKFFNGPIECVLKNYRISPKRPAVEVEPVAPKKTNKETPE
PINPWTMGQNESVVEAAIENESELVFKEVQQIKPAIYAEEFANRLLKNLKQLEKWAKREGVECYRLYDADLPEYNVAIDR
YAEYIIIQEYRAPKEIETQKVRRRFLDVVSTVRYVLNLPDDKLVIKVRERQKGRQQYEKLDTKKQSLVVHEGQAKMLVNL
QDYLDTGLFLDHRPTRLLIGNMAKGKDFLNLFCYTATASVHAALGGAKSTTSVDMSKTYLAWGEDNFAENGIKGKHEFIQ
QDCIKWLQHAHEMYDLIFIDPPTFSNSKRMNDVFDVQEDHVALLTSASQRLNKQGEIIFSNNKRGFKLDVDAIKALGFYI
KDISQSSIPEDFRRNKKIHQCWILTKHTD

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=748, Percent_Identity=49.8663101604278, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=234, Percent_Identity=28.2051282051282, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_PSYIN (A1SX09)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943623.1
- ProteinModelPortal:   A1SX09
- STRING:   A1SX09
- GeneID:   4626812
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2283
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   A1SX09
- ProtClustDB:   PRK11783
- BioCyc:   PING357804:PING_2283-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 85601; Mature: 85601

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYFIPTAKGLAPLLEVELKEMGIENPQQMNGGVKFEGTLEQGYKVCLWSRFASRVLLK
CCCEECCCHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCHHCCHHHHHHHHHHHHHHHH
LSEFKVLDSMDLYLGCSNIPWETHFDVDKTFSIDFSGTNDEIRNTQFGALKIKDAIVDRF
HHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCEEEHHHHHHHHH
RKHFDERPNVQKRDADIRFNGRLWKDKATIYLDLSGSPLHLRGYRTIAGEAPLRETLAAG
HHHHCCCCCCCCCCCCEEECCEEEECCEEEEEEECCCCEEEECEEEECCCCCHHHHHHHH
IIKRSGWQGEALLDPMCGSGTLVIEAAMMALNIAPGSLRETFGFEKWKKHDQECWQTLKT
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
SAQVYGRRAVNQCETRFYGSDLSKDMIEIARKNAQRAGVGEVIEFSVTDAKKVLPPEELE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCEEEEEECCHHHCCCHHHHC
TGMLITNPPYGERLGSFSDTITLYTELGYHFKDAFAGWNLSMFAMDTELLSCLGMRAGKS
CCEEEECCCCHHHHCCCCCCEEEEEHHCCHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCC
FKFFNGPIECVLKNYRISPKRPAVEVEPVAPKKTNKETPEPINPWTMGQNESVVEAAIEN
EEECCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
ESELVFKEVQQIKPAIYAEEFANRLLKNLKQLEKWAKREGVECYRLYDADLPEYNVAIDR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
YAEYIIIQEYRAPKEIETQKVRRRFLDVVSTVRYVLNLPDDKLVIKVRERQKGRQQYEKL
HHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHH
DTKKQSLVVHEGQAKMLVNLQDYLDTGLFLDHRPTRLLIGNMAKGKDFLNLFCYTATASV
CCHHHEEEEECCCCEEEEEHHHHHCCCCEECCCCCEEEECCCCCCCHHHHEEHHHHHHHH
HAALGGAKSTTSVDMSKTYLAWGEDNFAENGIKGKHEFIQQDCIKWLQHAHEMYDLIFID
HHHHCCCCCCCEECCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
PPTFSNSKRMNDVFDVQEDHVALLTSASQRLNKQGEIIFSNNKRGFKLDVDAIKALGFYI
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHH
KDISQSSIPEDFRRNKKIHQCWILTKHTD
HHCCCCCCCHHHHHCCCEEEEEEEEECCC
>Mature Secondary Structure
MLSYFIPTAKGLAPLLEVELKEMGIENPQQMNGGVKFEGTLEQGYKVCLWSRFASRVLLK
CCCEECCCHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCHHCCHHHHHHHHHHHHHHHH
LSEFKVLDSMDLYLGCSNIPWETHFDVDKTFSIDFSGTNDEIRNTQFGALKIKDAIVDRF
HHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCEEEHHHHHHHHH
RKHFDERPNVQKRDADIRFNGRLWKDKATIYLDLSGSPLHLRGYRTIAGEAPLRETLAAG
HHHHCCCCCCCCCCCCEEECCEEEECCEEEEEEECCCCEEEECEEEECCCCCHHHHHHHH
IIKRSGWQGEALLDPMCGSGTLVIEAAMMALNIAPGSLRETFGFEKWKKHDQECWQTLKT
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
SAQVYGRRAVNQCETRFYGSDLSKDMIEIARKNAQRAGVGEVIEFSVTDAKKVLPPEELE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCEEEEEECCHHHCCCHHHHC
TGMLITNPPYGERLGSFSDTITLYTELGYHFKDAFAGWNLSMFAMDTELLSCLGMRAGKS
CCEEEECCCCHHHHCCCCCCEEEEEHHCCHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCC
FKFFNGPIECVLKNYRISPKRPAVEVEPVAPKKTNKETPEPINPWTMGQNESVVEAAIEN
EEECCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
ESELVFKEVQQIKPAIYAEEFANRLLKNLKQLEKWAKREGVECYRLYDADLPEYNVAIDR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
YAEYIIIQEYRAPKEIETQKVRRRFLDVVSTVRYVLNLPDDKLVIKVRERQKGRQQYEKL
HHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHH
DTKKQSLVVHEGQAKMLVNLQDYLDTGLFLDHRPTRLLIGNMAKGKDFLNLFCYTATASV
CCHHHEEEEECCCCEEEEEHHHHHCCCCEECCCCCEEEECCCCCCCHHHHEEHHHHHHHH
HAALGGAKSTTSVDMSKTYLAWGEDNFAENGIKGKHEFIQQDCIKWLQHAHEMYDLIFID
HHHHCCCCCCCEECCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
PPTFSNSKRMNDVFDVQEDHVALLTSASQRLNKQGEIIFSNNKRGFKLDVDAIKALGFYI
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHH
KDISQSSIPEDFRRNKKIHQCWILTKHTD
HHCCCCCCCHHHHHCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA