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The map label for this gene is pleD [H]

Identifier: 119945933

GI number: 119945933

Start: 2762256

End: 2762591

Strand: Reverse

Name: pleD [H]

Synonym: Ping_2273

Alternate gene names: 119945933

Gene position: 2762591-2762256 (Counterclockwise)

Preceding gene: 119945934

Following gene: 119945932

Centisome position: 60.59

GC content: 38.69

Gene sequence:

>336_bases
ATGTATGATCAATTGACTGGACTCTCAAACCGCAGAAAATTCATGGAATTTGGTCAAGAAGAGATTAAGCGCGCCCAACG
TACTAAAAATAAACTTTGGCTTTTGATTTTGGATATTGATTATTTTAAAAAAATTAACGATACCGAAGGTCATGATATGG
GTGATCAATTACTTGTCACATTAAGTCAAATGCTTGAAAATTCTGTCTGCAGCATTGACCTTGTTAGCTGTATCGGGGGA
GAAGAGTTTTCTATTATACTGCTTGATACCAACAGACAGGGAGCTGCGCGCGTCGCTGAGAGTATCCGTATGAAAATAGA
AACTTCCCCAGCATAG

Upstream 100 bases:

>100_bases
TCTGTTGGTTTATTATATCAATGGTGAGTATTTCGGCTATTCTTCAAGAAAAGATCGATATTGAGCGCTGGGAAGGAGAA
CGTTTACTTGCTCAGCCGCC

Downstream 100 bases:

>100_bases
AAGGTTGGACCGATAGGTATGGGCCGGTGACTGTTTCAATTGGATGCGCTGTCAATGATGGTAGCTGTTCGCTGGAAGAA
ACCCCTAAAGTGGCAGATCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 111; Mature: 111

Protein sequence:

>111_residues
MYDQLTGLSNRRKFMEFGQEEIKRAQRTKNKLWLLILDIDYFKKINDTEGHDMGDQLLVTLSQMLENSVCSIDLVSCIGG
EEFSIILLDTNRQGAARVAESIRMKIETSPA

Sequences:

>Translated_111_residues
MYDQLTGLSNRRKFMEFGQEEIKRAQRTKNKLWLLILDIDYFKKINDTEGHDMGDQLLVTLSQMLENSVCSIDLVSCIGG
EEFSIILLDTNRQGAARVAESIRMKIETSPA
>Mature_111_residues
MYDQLTGLSNRRKFMEFGQEEIKRAQRTKNKLWLLILDIDYFKKINDTEGHDMGDQLLVTLSQMLENSVCSIDLVSCIGG
EEFSIILLDTNRQGAARVAESIRMKIETSPA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=107, Percent_Identity=42.9906542056075, Blast_Score=85, Evalue=1e-18,
Organism=Escherichia coli, GI1787262, Length=105, Percent_Identity=39.0476190476191, Blast_Score=82, Evalue=7e-18,
Organism=Escherichia coli, GI1786584, Length=113, Percent_Identity=35.3982300884956, Blast_Score=76, Evalue=6e-16,
Organism=Escherichia coli, GI87081881, Length=105, Percent_Identity=38.0952380952381, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI87082007, Length=105, Percent_Identity=36.1904761904762, Blast_Score=69, Evalue=6e-14,
Organism=Escherichia coli, GI87081974, Length=105, Percent_Identity=32.3809523809524, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787802, Length=110, Percent_Identity=34.5454545454545, Blast_Score=66, Evalue=5e-13,
Organism=Escherichia coli, GI1788381, Length=101, Percent_Identity=33.6633663366337, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787816, Length=100, Percent_Identity=40, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1788085, Length=107, Percent_Identity=29.9065420560748, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 12686; Mature: 12686

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDQLTGLSNRRKFMEFGQEEIKRAQRTKNKLWLLILDIDYFKKINDTEGHDMGDQLLVT
CCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCCCCHHHHHHH
LSQMLENSVCSIDLVSCIGGEEFSIILLDTNRQGAARVAESIRMKIETSPA
HHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MYDQLTGLSNRRKFMEFGQEEIKRAQRTKNKLWLLILDIDYFKKINDTEGHDMGDQLLVT
CCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCCCCHHHHHHH
LSQMLENSVCSIDLVSCIGGEEFSIILLDTNRQGAARVAESIRMKIETSPA
HHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA