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The map label for this gene is sgcA [H]

Identifier: 119945729

GI number: 119945729

Start: 2504743

End: 2505186

Strand: Reverse

Name: sgcA [H]

Synonym: Ping_2057

Alternate gene names: 119945729

Gene position: 2505186-2504743 (Counterclockwise)

Preceding gene: 119945731

Following gene: 119945728

Centisome position: 54.94

GC content: 40.54

Gene sequence:

>444_bases
ATGCTTAAAAAACTGCTTACAGAAGACGTTATAAGTATTTATGAAAGCGCTGAAAACTGGCAGCAGGCTATTAAGTTTGC
ATGTCAACCGCTGCTTGATAACGGCGCGATAGAGCCAAGTTATGTAGATGCGATTTTTAAGTGCCATCAGGAAATCGGTC
CTTATTATGTTATTGGTCCTGGCATTGCGATGCCTCATGCAAGACCGGAAGACGGTGTTAATAGACTGTCTTTAGGTTTA
ACGGTTATTAAAAATGGCGTTAACTTTAATAATGAAGAGAACGATCCGGTAAAGCTGTTAGTGACGCTCGCGGCGACAGA
CAGTGACAGTCACGTTATAGCCATTCAGCAGTTGATGGAATTATTTATGAACGAAGAGCATATCGAAGCAATCTGCAATG
CATCAAATGTTGCTGAAATTCGCAGTATTATAGAGCAGTATTAA

Upstream 100 bases:

>100_bases
ATAGCGCTATCAAATGTGATTTTAGTCACAAAACGAATCTATAATAGGTTTAAAGTTACGCCATTAATCAACCACAGCTT
AACAACAGGAGCATAAAAAC

Downstream 100 bases:

>100_bases
TCAGTTTCTTGTCAATAATGACAGATTAGTTAACAACACTAAGTTAAAAACAATAGAGGTATAAATTATGAACATTTTAG
TTGTATGTGGGAATGGTCTT

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLKKLLTEDVISIYESAENWQQAIKFACQPLLDNGAIEPSYVDAIFKCHQEIGPYYVIGPGIAMPHARPEDGVNRLSLGL
TVIKNGVNFNNEENDPVKLLVTLAATDSDSHVIAIQQLMELFMNEEHIEAICNASNVAEIRSIIEQY

Sequences:

>Translated_147_residues
MLKKLLTEDVISIYESAENWQQAIKFACQPLLDNGAIEPSYVDAIFKCHQEIGPYYVIGPGIAMPHARPEDGVNRLSLGL
TVIKNGVNFNNEENDPVKLLVTLAATDSDSHVIAIQQLMELFMNEEHIEAICNASNVAEIRSIIEQY
>Mature_147_residues
MLKKLLTEDVISIYESAENWQQAIKFACQPLLDNGAIEPSYVDAIFKCHQEIGPYYVIGPGIAMPHARPEDGVNRLSLGL
TVIKNGVNFNNEENDPVKLLVTLAATDSDSHVIAIQQLMELFMNEEHIEAICNASNVAEIRSIIEQY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790755, Length=122, Percent_Identity=41.8032786885246, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI2367359, Length=137, Percent_Identity=37.2262773722628, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1789302, Length=145, Percent_Identity=42.0689655172414, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=29.6551724137931, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16372; Mature: 16372

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLLTEDVISIYESAENWQQAIKFACQPLLDNGAIEPSYVDAIFKCHQEIGPYYVIGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECC
GIAMPHARPEDGVNRLSLGLTVIKNGVNFNNEENDPVKLLVTLAATDSDSHVIAIQQLME
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
LFMNEEHIEAICNASNVAEIRSIIEQY
HHCCHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLKKLLTEDVISIYESAENWQQAIKFACQPLLDNGAIEPSYVDAIFKCHQEIGPYYVIGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECC
GIAMPHARPEDGVNRLSLGLTVIKNGVNFNNEENDPVKLLVTLAATDSDSHVIAIQQLME
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
LFMNEEHIEAICNASNVAEIRSIIEQY
HHCCHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]