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The map label for this gene is lacI [H]

Identifier: 119945696

GI number: 119945696

Start: 2448811

End: 2449881

Strand: Reverse

Name: lacI [H]

Synonym: Ping_2013

Alternate gene names: 119945696

Gene position: 2449881-2448811 (Counterclockwise)

Preceding gene: 119945703

Following gene: 119945693

Centisome position: 53.73

GC content: 44.35

Gene sequence:

>1071_bases
ATGAATGTGACATTTAAGGACGTAGCTGAGCTGGCTAACGTATCAACGCAAACTGTTTCGCGTGTTACCAACGGTTCGCT
GAACGTTGCCCCTGAAACCAGAGAGAAAGTTAATGCGGCTATTAAACAATTGGGATATATCCCTAATAAAGGTGCGCAAA
TATTAGGGCGGGCAAAGTCAAAAATGATTGGTCTCGTGACCCTGGATATTTTGTTACATGGCGCATCATTGCTTGCCAAT
GGTGTGCGTAAGCAGGCAAAAAATCTGGGTTACGGTACTTCCTTATCCGTTATTGCTGATCCTGCTATTGATAAAATAAC
GACAGCAGTCGATGATCTCAAAGCGCAACGTGTGGAATTTATTATTATGAATATTCCACTGAGCAGTGATAATGCTGAAA
TGTTGGTTGTGCAGTATTCGAGTATTCATTTTGTGTTTATTGATGTGCCACCAGACAGCCAGGTCAACTCGGTATCAGGC
GCTAGCTATAATGGTGCAAAAATAGCGGCTGAATTGATGCTATCGTTGTGCCGAAAACGCCTATTATTCATTAACGGTCC
TCAGGGCTCCAGTGCATCGCGACTTCGCCTGCAGGGCTGGTTAGATGTTTTAAAAGAGGATGCGGACAGCAAAATTATTA
ATCAGTTTGAAGGAGACTGGCGAGCTAGCAGCGGTTATCGGTGCATGCGTCAAGCGCTCAGCGACGGTCACGATTTCGAC
GGTGTGCTGGTGGCCAATGACCAAATGGCGCTGGGTGTACTAAGGGCATTAAGTGAATTTAATTTAAGCGTACCCCAGCA
AGTATCCGTTATTGGTTTCGATGATACTGTCGATAGCGCTTATTTCACGCCACCATTGACCACTATTAAGCAGGACTTTT
TAACCATTGGCGAACGCGCTGTTTTACTTGCCTTTAGCGTGGCGGATAAAGCAAAAAAATCCTACCGGCATGAGTCTATT
GTGACCGAGTTGATTGAGCGAAAAAGTACGCAAGCTTATCGACCCCCGCAGGCTAATGAGCAAAAAATTAAGGTGCTGCT
TAAGCAAATTGAAAAGTTACTTATTGAATAA

Upstream 100 bases:

>100_bases
AGGAACGCTATAATTGCAAGCAATACAGTGTTAAGTGCTCGTCTCGAGCAGGGATCAATATTATAATTTTTCATGATTTT
AAGAGGGTTGGAGGCAGATA

Downstream 100 bases:

>100_bases
CCTGTATGAGTCAAACAAAAAATAAAGCGGATTGAGTCGCCATTAGGCTACTCATCTGCAAGCCCTTTTACTTTTATAAC
ATCGGCGTCGCTATTGAAAT

Product: lac repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNVTFKDVAELANVSTQTVSRVTNGSLNVAPETREKVNAAIKQLGYIPNKGAQILGRAKSKMIGLVTLDILLHGASLLAN
GVRKQAKNLGYGTSLSVIADPAIDKITTAVDDLKAQRVEFIIMNIPLSSDNAEMLVVQYSSIHFVFIDVPPDSQVNSVSG
ASYNGAKIAAELMLSLCRKRLLFINGPQGSSASRLRLQGWLDVLKEDADSKIINQFEGDWRASSGYRCMRQALSDGHDFD
GVLVANDQMALGVLRALSEFNLSVPQQVSVIGFDDTVDSAYFTPPLTTIKQDFLTIGERAVLLAFSVADKAKKSYRHESI
VTELIERKSTQAYRPPQANEQKIKVLLKQIEKLLIE

Sequences:

>Translated_356_residues
MNVTFKDVAELANVSTQTVSRVTNGSLNVAPETREKVNAAIKQLGYIPNKGAQILGRAKSKMIGLVTLDILLHGASLLAN
GVRKQAKNLGYGTSLSVIADPAIDKITTAVDDLKAQRVEFIIMNIPLSSDNAEMLVVQYSSIHFVFIDVPPDSQVNSVSG
ASYNGAKIAAELMLSLCRKRLLFINGPQGSSASRLRLQGWLDVLKEDADSKIINQFEGDWRASSGYRCMRQALSDGHDFD
GVLVANDQMALGVLRALSEFNLSVPQQVSVIGFDDTVDSAYFTPPLTTIKQDFLTIGERAVLLAFSVADKAKKSYRHESI
VTELIERKSTQAYRPPQANEQKIKVLLKQIEKLLIE
>Mature_356_residues
MNVTFKDVAELANVSTQTVSRVTNGSLNVAPETREKVNAAIKQLGYIPNKGAQILGRAKSKMIGLVTLDILLHGASLLAN
GVRKQAKNLGYGTSLSVIADPAIDKITTAVDDLKAQRVEFIIMNIPLSSDNAEMLVVQYSSIHFVFIDVPPDSQVNSVSG
ASYNGAKIAAELMLSLCRKRLLFINGPQGSSASRLRLQGWLDVLKEDADSKIINQFEGDWRASSGYRCMRQALSDGHDFD
GVLVANDQMALGVLRALSEFNLSVPQQVSVIGFDDTVDSAYFTPPLTTIKQDFLTIGERAVLLAFSVADKAKKSYRHESI
VTELIERKSTQAYRPPQANEQKIKVLLKQIEKLLIE

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=357, Percent_Identity=37.2549019607843, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1790194, Length=303, Percent_Identity=32.3432343234323, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=31.7647058823529, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788474, Length=339, Percent_Identity=28.9085545722714, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789068, Length=300, Percent_Identity=30.6666666666667, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=28, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=28.7833827893175, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=24.6056782334385, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=25.077399380805, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786268, Length=311, Percent_Identity=22.508038585209, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38953; Mature: 38953

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTFKDVAELANVSTQTVSRVTNGSLNVAPETREKVNAAIKQLGYIPNKGAQILGRAKS
CCCCHHHHHHHHCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KMIGLVTLDILLHGASLLANGVRKQAKNLGYGTSLSVIADPAIDKITTAVDDLKAQRVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHEEECCHHHHHHHHHHHHHHHEEEE
IIMNIPLSSDNAEMLVVQYSSIHFVFIDVPPDSQVNSVSGASYNGAKIAAELMLSLCRKR
EEEEECCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LLFINGPQGSSASRLRLQGWLDVLKEDADSKIINQFEGDWRASSGYRCMRQALSDGHDFD
EEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
GVLVANDQMALGVLRALSEFNLSVPQQVSVIGFDDTVDSAYFTPPLTTIKQDFLTIGERA
CEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHH
VLLAFSVADKAKKSYRHESIVTELIERKSTQAYRPPQANEQKIKVLLKQIEKLLIE
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVTFKDVAELANVSTQTVSRVTNGSLNVAPETREKVNAAIKQLGYIPNKGAQILGRAKS
CCCCHHHHHHHHCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KMIGLVTLDILLHGASLLANGVRKQAKNLGYGTSLSVIADPAIDKITTAVDDLKAQRVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHEEECCHHHHHHHHHHHHHHHEEEE
IIMNIPLSSDNAEMLVVQYSSIHFVFIDVPPDSQVNSVSGASYNGAKIAAELMLSLCRKR
EEEEECCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LLFINGPQGSSASRLRLQGWLDVLKEDADSKIINQFEGDWRASSGYRCMRQALSDGHDFD
EEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
GVLVANDQMALGVLRALSEFNLSVPQQVSVIGFDDTVDSAYFTPPLTTIKQDFLTIGERA
CEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHH
VLLAFSVADKAKKSYRHESIVTELIERKSTQAYRPPQANEQKIKVLLKQIEKLLIE
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]