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The map label for this gene is msbB [H]

Identifier: 119945661

GI number: 119945661

Start: 2401486

End: 2402451

Strand: Reverse

Name: msbB [H]

Synonym: Ping_1974

Alternate gene names: 119945661

Gene position: 2402451-2401486 (Counterclockwise)

Preceding gene: 119945662

Following gene: 229358029

Centisome position: 52.69

GC content: 38.51

Gene sequence:

>966_bases
ATGACTCAATACCAATCTAGATTTGACACCTCCTTGAAAAAACAGCACTTTCTTCCAAAATATTGGGGAACATGGCTTTT
GCTGGGTTTATTATTTCTATTCAGTTATTTACCCATAAAATGGCGAGATTGTTTTGCCACATTTATTGCTAATAAATTAT
ATGACACTAAATTTCTAAATAAACGCAAAAAAATTGCATTTATCAATTTGGGATTATGTTTTCCGGAATATAACGAAGCT
GACAAAGATGCGCTGATGCGTGAAAACTTCCGCGCGGTTACACAAATCTTATTATCACTCGGTGAACTTGCTTTTCGCAG
TCAGGCATTTTTAATTAAACGCATCAATTTTATTGGTGAGCATTATATTAAAGAAGCTGAAGCACAGGATAAAGCCATTA
TTTTCTTGGCACCGCATTGTTACGGTTTAGATTTTGCAGGGGCAGCCGCTATTTCCGCAAGAGGTTACCCGCTATCAAGT
ATGTTTAAAGATTATAATAATCCGATCTTTGACTGGTTTGTAACGGGTTATCGAACTCGTTTTACCAAGCTTGCCGGAAA
GGGAACCTTGTATCATCGTTCTGAAGGTTTAAAGCCTGCGATTAAAAGTTTACGCGATAAAGCCCATTTTTATTATTTAC
CCGATGAAGATCATGGGCGCGAAAACAGTATTTTTACTGATTTTTATGCTACTAAAAAAGCCACCCTGCCAGTGATTGGG
CGATTGGCTAAATTAGGGCGTGCTGTGGTCATTCCTGTTTATACTTCATACAATGCAAACACCTCAAAAATTGATGTCAT
TTGCCATGCCCCGCTGCAAAATTTCCCCCAGGGTGATGAACTGCAACAGACCGAGTTAATGAATCGTGCCATTGAAAAAG
TGATCGATGAAAACAGAGCGCAGTACATGTGGACACTTAAGCTATTAAAAACCCGTCCTGAGGAAGGAGTCGATATCTAC
AAATAA

Upstream 100 bases:

>100_bases
GGCAGCAGCCATAGCAAGCGATTCAAGTGCCGAGGGTTAGCTGGGAAGTCTACCACCAACATCCCCCTAAAATAATACCT
TGTTTCCCGAAGGTCTAATA

Downstream 100 bases:

>100_bases
GTTTTTGGGGGGCGGTTCAAAATACCCCCTTTTTTGTTTTTGTACTTATCTAAACCGTTAATTCTCAGACAGTAATATAG
CTTTACTGTGCATTGATTCG

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTQYQSRFDTSLKKQHFLPKYWGTWLLLGLLFLFSYLPIKWRDCFATFIANKLYDTKFLNKRKKIAFINLGLCFPEYNEA
DKDALMRENFRAVTQILLSLGELAFRSQAFLIKRINFIGEHYIKEAEAQDKAIIFLAPHCYGLDFAGAAAISARGYPLSS
MFKDYNNPIFDWFVTGYRTRFTKLAGKGTLYHRSEGLKPAIKSLRDKAHFYYLPDEDHGRENSIFTDFYATKKATLPVIG
RLAKLGRAVVIPVYTSYNANTSKIDVICHAPLQNFPQGDELQQTELMNRAIEKVIDENRAQYMWTLKLLKTRPEEGVDIY
K

Sequences:

>Translated_321_residues
MTQYQSRFDTSLKKQHFLPKYWGTWLLLGLLFLFSYLPIKWRDCFATFIANKLYDTKFLNKRKKIAFINLGLCFPEYNEA
DKDALMRENFRAVTQILLSLGELAFRSQAFLIKRINFIGEHYIKEAEAQDKAIIFLAPHCYGLDFAGAAAISARGYPLSS
MFKDYNNPIFDWFVTGYRTRFTKLAGKGTLYHRSEGLKPAIKSLRDKAHFYYLPDEDHGRENSIFTDFYATKKATLPVIG
RLAKLGRAVVIPVYTSYNANTSKIDVICHAPLQNFPQGDELQQTELMNRAIEKVIDENRAQYMWTLKLLKTRPEEGVDIY
K
>Mature_320_residues
TQYQSRFDTSLKKQHFLPKYWGTWLLLGLLFLFSYLPIKWRDCFATFIANKLYDTKFLNKRKKIAFINLGLCFPEYNEAD
KDALMRENFRAVTQILLSLGELAFRSQAFLIKRINFIGEHYIKEAEAQDKAIIFLAPHCYGLDFAGAAAISARGYPLSSM
FKDYNNPIFDWFVTGYRTRFTKLAGKGTLYHRSEGLKPAIKSLRDKAHFYYLPDEDHGRENSIFTDFYATKKATLPVIGR
LAKLGRAVVIPVYTSYNANTSKIDVICHAPLQNFPQGDELQQTELMNRAIEKVIDENRAQYMWTLKLLKTRPEEGVDIYK

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=320, Percent_Identity=34.6875, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1787292, Length=312, Percent_Identity=29.4871794871795, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082094, Length=311, Percent_Identity=27.0096463022508, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37231; Mature: 37100

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQYQSRFDTSLKKQHFLPKYWGTWLLLGLLFLFSYLPIKWRDCFATFIANKLYDTKFLN
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
KRKKIAFINLGLCFPEYNEADKDALMRENFRAVTQILLSLGELAFRSQAFLIKRINFIGE
CCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYIKEAEAQDKAIIFLAPHCYGLDFAGAAAISARGYPLSSMFKDYNNPIFDWFVTGYRTR
HHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
FTKLAGKGTLYHRSEGLKPAIKSLRDKAHFYYLPDEDHGRENSIFTDFYATKKATLPVIG
HHHHHCCCCEEECCCCCHHHHHHHHHCCEEEECCCCCCCCCCCEEEHHHHCCCCHHHHHH
RLAKLGRAVVIPVYTSYNANTSKIDVICHAPLQNFPQGDELQQTELMNRAIEKVIDENRA
HHHHCCCEEEEEEEECCCCCCCEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHHCCCH
QYMWTLKLLKTRPEEGVDIYK
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TQYQSRFDTSLKKQHFLPKYWGTWLLLGLLFLFSYLPIKWRDCFATFIANKLYDTKFLN
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
KRKKIAFINLGLCFPEYNEADKDALMRENFRAVTQILLSLGELAFRSQAFLIKRINFIGE
CCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYIKEAEAQDKAIIFLAPHCYGLDFAGAAAISARGYPLSSMFKDYNNPIFDWFVTGYRTR
HHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
FTKLAGKGTLYHRSEGLKPAIKSLRDKAHFYYLPDEDHGRENSIFTDFYATKKATLPVIG
HHHHHCCCCEEECCCCCHHHHHHHHHCCEEEECCCCCCCCCCCEEEHHHHCCCCHHHHHH
RLAKLGRAVVIPVYTSYNANTSKIDVICHAPLQNFPQGDELQQTELMNRAIEKVIDENRA
HHHHCCCEEEEEEEECCCCCCCEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHHCCCH
QYMWTLKLLKTRPEEGVDIYK
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]