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The map label for this gene is pfaD [H]

Identifier: 119945398

GI number: 119945398

Start: 2059702

End: 2061297

Strand: Direct

Name: pfaD [H]

Synonym: Ping_1687

Alternate gene names: 119945398

Gene position: 2059702-2061297 (Clockwise)

Preceding gene: 119945397

Following gene: 119945400

Centisome position: 45.17

GC content: 48.75

Gene sequence:

>1596_bases
ATGTCAGCTTTAGGTTTTAATACAGACAATCAAATTAACTGGGTATGGAAAGTCAGTCCGGCAACCATAAACAGTGATCC
GCAGGCTATTAAAGATTGCTTAATGGATTTGCACAAACCCGTCTACCTTGTACAACAGGCTGAAAGTTTAGGGGTAAGTC
AGCAGATTGACGCCGATGGCAGTGAGGCAGTACTCGCCTTTGCACAGGCGCTTAATCCGGAAGATCTCGGCGATCCGGCC
TTTAAGAAGAGCTACGGTCTAAAATATGCCTATCACGGCGGGGCGATGGCCAATGGTATCTCTTCCGTAGAATTAGTCAA
GGCCCTCGGCAAAGCAGGATTTTTATGCTCCTTTGGTGCGGCGGGTTTAGTGCCCGACGCGCTTGAAAAGGCAATCAAAG
CTATTCAAACGGCCTTACCAAACGGCCCCTTTGCGGTCAACCTGATTCATGCTCCCAGCGAAGAAGCCTTAGAGCGCGGC
GCCGTTGAACGTTTTCTAAAACTCGGTGTTAAAGTGGTCGAAGCATCGGCCTATTTAGCCTTAACTGAGCATATTGTCTA
CTACCGTTTAGCCGGTTTATCGGAAAATGCCGATGGCAGCGTTAATATCGGCAACAAAGTGATTGCCAAGGTATCGCGCA
CTGAAGTCGGACGGCGTTTTATGGAGCCGGCACCGAAAAAGTTGGTTGATAAATTACTTGTACAGGGCAAAATTACACCT
CTGCAGGCAGCGCTTTCAGAGCGTGTGCCGATGGCCGACGACATCACCGCCGAAGCTGATTCCGGCGGGCACACGGATAA
CCGTCCGTTTGTAACTTTGCTACCTTCTATTATCGCTCTGCGTGATGAAATTCAGGAAAAATACAATTTTTCACCTGCTC
TGCGGATTGGCGCTGGTGGGGGAATAGGTACCCCGCAGGCAACTCTGGCGGCGTTTAATATGGGGGCTGCCTACATTGTT
GTCGGCAGTGTTAATCAGGCCTGTATTGAAGCTGGCGCCTCGGAGCATACGCGCAAATTATTAGCCGATGTTGAGATGGC
CGATGTGACCATGGCGCCCGCCGCAGATATGTTTGAAATGGGGGTTAAATTACAAGTGGTTAAACGTGGCTCGATGTTTG
CCATGCGCGCCAATAAACTCTATGACTTGTATATGGCTTATCCGTCAATCGAAGCGATTCCAACCCTTGAGCGTGAGAAA
ATTGAAAAACAGATTTTTCGCAAAAACTTAGATGCAGTGTGGGCAGATACTATCGCTTTTTTCCACGAGCGCGATCCCGC
CATGCTCGAGCGCGCGCAAACTAACCCTAAACGTAAAATGGCACTGATTTTCCGCTGGTACTTAGGACTTTCATCGCGTT
GGTCGAACGAAGGAGAAGCTGGCCGTGAAATGGATTATCAAATATGGGCAGGCCCAAGTTTAGGTGCGTTTAATAGTTGG
GTGAAGGGCAGTTATTTAGAGGACTACACACAGCGTAAAGCGGTTGATGTGGCGCTGCATCTGTTAAAAGGCAGTGCCTA
TTTACAACGAATTAATCAGCTTAAACTGCAGGGCGTTACATTAGCGCAAAGTTTGTCGGGGTATTACCCGAGCTAA

Upstream 100 bases:

>100_bases
ATCCTCAGCAGACTGTTTGCTGGAATCTTAAAACGTTTTATATGACGTTAAATTACAAGCACCAAACGCAGGTCAATCCT
GCTTCGTTTAAGGTACAAAT

Downstream 100 bases:

>100_bases
AAACTGCTTTGTGGTGTGCTGATTTATCAGCACAGGGTGATCTAAAAAGCACTCATAAATTTATTGTTAGCCTCAAGTTG
TTTAATGCTTGGGGTTTTTT

Product: polyunsaturated fatty acid synthase PfaD

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSALGFNTDNQINWVWKVSPATINSDPQAIKDCLMDLHKPVYLVQQAESLGVSQQIDADGSEAVLAFAQALNPEDLGDPA
FKKSYGLKYAYHGGAMANGISSVELVKALGKAGFLCSFGAAGLVPDALEKAIKAIQTALPNGPFAVNLIHAPSEEALERG
AVERFLKLGVKVVEASAYLALTEHIVYYRLAGLSENADGSVNIGNKVIAKVSRTEVGRRFMEPAPKKLVDKLLVQGKITP
LQAALSERVPMADDITAEADSGGHTDNRPFVTLLPSIIALRDEIQEKYNFSPALRIGAGGGIGTPQATLAAFNMGAAYIV
VGSVNQACIEAGASEHTRKLLADVEMADVTMAPAADMFEMGVKLQVVKRGSMFAMRANKLYDLYMAYPSIEAIPTLEREK
IEKQIFRKNLDAVWADTIAFFHERDPAMLERAQTNPKRKMALIFRWYLGLSSRWSNEGEAGREMDYQIWAGPSLGAFNSW
VKGSYLEDYTQRKAVDVALHLLKGSAYLQRINQLKLQGVTLAQSLSGYYPS

Sequences:

>Translated_531_residues
MSALGFNTDNQINWVWKVSPATINSDPQAIKDCLMDLHKPVYLVQQAESLGVSQQIDADGSEAVLAFAQALNPEDLGDPA
FKKSYGLKYAYHGGAMANGISSVELVKALGKAGFLCSFGAAGLVPDALEKAIKAIQTALPNGPFAVNLIHAPSEEALERG
AVERFLKLGVKVVEASAYLALTEHIVYYRLAGLSENADGSVNIGNKVIAKVSRTEVGRRFMEPAPKKLVDKLLVQGKITP
LQAALSERVPMADDITAEADSGGHTDNRPFVTLLPSIIALRDEIQEKYNFSPALRIGAGGGIGTPQATLAAFNMGAAYIV
VGSVNQACIEAGASEHTRKLLADVEMADVTMAPAADMFEMGVKLQVVKRGSMFAMRANKLYDLYMAYPSIEAIPTLEREK
IEKQIFRKNLDAVWADTIAFFHERDPAMLERAQTNPKRKMALIFRWYLGLSSRWSNEGEAGREMDYQIWAGPSLGAFNSW
VKGSYLEDYTQRKAVDVALHLLKGSAYLQRINQLKLQGVTLAQSLSGYYPS
>Mature_530_residues
SALGFNTDNQINWVWKVSPATINSDPQAIKDCLMDLHKPVYLVQQAESLGVSQQIDADGSEAVLAFAQALNPEDLGDPAF
KKSYGLKYAYHGGAMANGISSVELVKALGKAGFLCSFGAAGLVPDALEKAIKAIQTALPNGPFAVNLIHAPSEEALERGA
VERFLKLGVKVVEASAYLALTEHIVYYRLAGLSENADGSVNIGNKVIAKVSRTEVGRRFMEPAPKKLVDKLLVQGKITPL
QAALSERVPMADDITAEADSGGHTDNRPFVTLLPSIIALRDEIQEKYNFSPALRIGAGGGIGTPQATLAAFNMGAAYIVV
GSVNQACIEAGASEHTRKLLADVEMADVTMAPAADMFEMGVKLQVVKRGSMFAMRANKLYDLYMAYPSIEAIPTLEREKI
EKQIFRKNLDAVWADTIAFFHERDPAMLERAQTNPKRKMALIFRWYLGLSSRWSNEGEAGREMDYQIWAGPSLGAFNSWV
KGSYLEDYTQRKAVDVALHLLKGSAYLQRINQLKLQGVTLAQSLSGYYPS

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 57797; Mature: 57666

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALGFNTDNQINWVWKVSPATINSDPQAIKDCLMDLHKPVYLVQQAESLGVSQQIDADG
CCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
SEAVLAFAQALNPEDLGDPAFKKSYGLKYAYHGGAMANGISSVELVKALGKAGFLCSFGA
HHHHHHHHHHCCCCCCCCCHHHHHCCCEEEECCCHHHCCCHHHHHHHHHCCCCCEECCCC
AGLVPDALEKAIKAIQTALPNGPFAVNLIHAPSEEALERGAVERFLKLGVKVVEASAYLA
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
LTEHIVYYRLAGLSENADGSVNIGNKVIAKVSRTEVGRRFMEPAPKKLVDKLLVQGKITP
HHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
LQAALSERVPMADDITAEADSGGHTDNRPFVTLLPSIIALRDEIQEKYNFSPALRIGAGG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GIGTPQATLAAFNMGAAYIVVGSVNQACIEAGASEHTRKLLADVEMADVTMAPAADMFEM
CCCCCHHHHHHHCCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHC
GVKLQVVKRGSMFAMRANKLYDLYMAYPSIEAIPTLEREKIEKQIFRKNLDAVWADTIAF
CCEEEEEECCCEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FHERDPAMLERAQTNPKRKMALIFRWYLGLSSRWSNEGEAGREMDYQIWAGPSLGAFNSW
HHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHH
VKGSYLEDYTQRKAVDVALHLLKGSAYLQRINQLKLQGVTLAQSLSGYYPS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SALGFNTDNQINWVWKVSPATINSDPQAIKDCLMDLHKPVYLVQQAESLGVSQQIDADG
CCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
SEAVLAFAQALNPEDLGDPAFKKSYGLKYAYHGGAMANGISSVELVKALGKAGFLCSFGA
HHHHHHHHHHCCCCCCCCCHHHHHCCCEEEECCCHHHCCCHHHHHHHHHCCCCCEECCCC
AGLVPDALEKAIKAIQTALPNGPFAVNLIHAPSEEALERGAVERFLKLGVKVVEASAYLA
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
LTEHIVYYRLAGLSENADGSVNIGNKVIAKVSRTEVGRRFMEPAPKKLVDKLLVQGKITP
HHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
LQAALSERVPMADDITAEADSGGHTDNRPFVTLLPSIIALRDEIQEKYNFSPALRIGAGG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GIGTPQATLAAFNMGAAYIVVGSVNQACIEAGASEHTRKLLADVEMADVTMAPAADMFEM
CCCCCHHHHHHHCCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHC
GVKLQVVKRGSMFAMRANKLYDLYMAYPSIEAIPTLEREKIEKQIFRKNLDAVWADTIAF
CCEEEEEECCCEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FHERDPAMLERAQTNPKRKMALIFRWYLGLSSRWSNEGEAGREMDYQIWAGPSLGAFNSW
HHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHH
VKGSYLEDYTQRKAVDVALHLLKGSAYLQRINQLKLQGVTLAQSLSGYYPS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]