Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 119945190

GI number: 119945190

Start: 1792206

End: 1793834

Strand: Reverse

Name: eptA [H]

Synonym: Ping_1454

Alternate gene names: 119945190

Gene position: 1793834-1792206 (Counterclockwise)

Preceding gene: 119945206

Following gene: 119945189

Centisome position: 39.34

GC content: 36.16

Gene sequence:

>1629_bases
ATGGTGTTTTTGATCACCCTTCTTTCAATATACTATGGCTTTGTTTTCAATTACCCTATTATCGATAAAATCTATATTTT
ATCGGACAATCAGAGTGTCTTTTATTTTCTGTCACCGCTCGTTTTAACTTTTGCATTTATTATTATTTTTTCAATACTCG
CCCTGCCCTATCTATTTAAAGCAATACAAATCCCACTGCTGATAACATCTGCATTAGCTTTTTATGCCAGTATCAAATAT
AACGTCATGTTTGACTACTCTATGATAGAAAATATTTTTGAAACAGATACGGGGGAAGCTTTTACTTATATAAACTTTGG
GTCAATTTCATACTTTATATTCTTAGGCCTACTACCTGCATTGATTGTTTTTAAAACAAAAATAACCTATGCAAAAAGTA
TTACCAGAGAAATAATTAATCGGCTTTTATTGGTTATTGTTGCCGTTGTTGGGATTTTGTTTATCGCCTTTTTTTATTAC
AAAGATTATGCCTCTATAGGGCGAAATAACAGCTATCTAAACAAAATGATTAACCCGGCTCATGCATACAATTCATATAA
GTATCTGAAGATAACTTATTTAACAAAAGAATTGGCTTACAAAAAAATTGGCCAGGATGCGAGTCTGGAAAACAGCAGTA
ATGACAAACCTACTTTAATGATTTTAGTCGTGGGTGAGACAGCACGTTCACAAAATATTTTTTACAATGGTTATGCGCGT
AATACCAATCCCCATACACAAGATATGGGGATAATTTCTTTTCAAGATGTTTCCTCATGCGGCACAGCGACTGCACACTC
GTTACCCTGTATGTTTTCAAATATGAACCGCTCTAATTACGACAAAAATCGTGCAATTACCCAGGATAATGCTCTGGATG
TTATTTCTCATGCAGGCGTGGATATGCTCTGGATTGAAAATGATGGCGGTGATAAAAGAGTCGCTGACAATTTTTCAAAA
ATAGAGATCGCAGCGGAAGACAGCCCGGAGTTATGTGATGCAGGATCCTGTTACGATGAGGTTTTACTGCAAAATCTAGA
CGAAAAAATTCAAAAAAATAAAGGTAATCAATTGATCGCTTTACATATTAAAGGCAGTCATGGACCGACCTACTGGCAGC
GCTATCCGGAAAGTAAAGCACGATTCACCCCCGCTTGTAATCAAAGTGATATTGAACACTGCAGCGATCAAGAGATAGTC
AATGTTTATGATAATACCCTTGTTTATACGGATTATATTATTGCCCAGACCATTAAAAAATTAACAGAATATTCGAAGCA
ATATAATGTTGCCTTAATGTATATCTCAGACCATGGCGAATCGCTTGGTGAAAATGGCTTATATCTGCATGGTGCTCCTT
ATATGATTGCCCCTGAAGAACAAACTCAGGTTCCCTGGTTTATGTGGTTAGGAAATGGTTATGCCGCTGCAAAAGGGATC
GATAAACAGTGTCTGCTTGATAAAGCAAACAATGATACCTTTTCTCACGATAATCTGTTCCATACTCTCCTCGGATTCTA
TGGTGTTGATTCAACAGCAAAAGATAGTCAACTGGATATTATTGCCAGTTGCAAACAGAGCATAAGTAATATTGCAAAAA
CGAAGTCAGTCGATCCTGAAATAAATTAG

Upstream 100 bases:

>100_bases
TTTAAAAAAGTATACTATTACTTATTATTAACCCATCGGGATAACCACCTTGAAATCGACTTTGTTAAAGACCATTAAAA
ATATCAATCTCTCCTCAAAT

Downstream 100 bases:

>100_bases
CACTGAAGGCTAAATAACTCTCCCGCGAGGAAGTAAGATGATACCAGTAACGTACTCTGATTATTAAGTCGTTAATACGG
CCTTGGAATGATATCCATGG

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MVFLITLLSIYYGFVFNYPIIDKIYILSDNQSVFYFLSPLVLTFAFIIIFSILALPYLFKAIQIPLLITSALAFYASIKY
NVMFDYSMIENIFETDTGEAFTYINFGSISYFIFLGLLPALIVFKTKITYAKSITREIINRLLLVIVAVVGILFIAFFYY
KDYASIGRNNSYLNKMINPAHAYNSYKYLKITYLTKELAYKKIGQDASLENSSNDKPTLMILVVGETARSQNIFYNGYAR
NTNPHTQDMGIISFQDVSSCGTATAHSLPCMFSNMNRSNYDKNRAITQDNALDVISHAGVDMLWIENDGGDKRVADNFSK
IEIAAEDSPELCDAGSCYDEVLLQNLDEKIQKNKGNQLIALHIKGSHGPTYWQRYPESKARFTPACNQSDIEHCSDQEIV
NVYDNTLVYTDYIIAQTIKKLTEYSKQYNVALMYISDHGESLGENGLYLHGAPYMIAPEEQTQVPWFMWLGNGYAAAKGI
DKQCLLDKANNDTFSHDNLFHTLLGFYGVDSTAKDSQLDIIASCKQSISNIAKTKSVDPEIN

Sequences:

>Translated_542_residues
MVFLITLLSIYYGFVFNYPIIDKIYILSDNQSVFYFLSPLVLTFAFIIIFSILALPYLFKAIQIPLLITSALAFYASIKY
NVMFDYSMIENIFETDTGEAFTYINFGSISYFIFLGLLPALIVFKTKITYAKSITREIINRLLLVIVAVVGILFIAFFYY
KDYASIGRNNSYLNKMINPAHAYNSYKYLKITYLTKELAYKKIGQDASLENSSNDKPTLMILVVGETARSQNIFYNGYAR
NTNPHTQDMGIISFQDVSSCGTATAHSLPCMFSNMNRSNYDKNRAITQDNALDVISHAGVDMLWIENDGGDKRVADNFSK
IEIAAEDSPELCDAGSCYDEVLLQNLDEKIQKNKGNQLIALHIKGSHGPTYWQRYPESKARFTPACNQSDIEHCSDQEIV
NVYDNTLVYTDYIIAQTIKKLTEYSKQYNVALMYISDHGESLGENGLYLHGAPYMIAPEEQTQVPWFMWLGNGYAAAKGI
DKQCLLDKANNDTFSHDNLFHTLLGFYGVDSTAKDSQLDIIASCKQSISNIAKTKSVDPEIN
>Mature_542_residues
MVFLITLLSIYYGFVFNYPIIDKIYILSDNQSVFYFLSPLVLTFAFIIIFSILALPYLFKAIQIPLLITSALAFYASIKY
NVMFDYSMIENIFETDTGEAFTYINFGSISYFIFLGLLPALIVFKTKITYAKSITREIINRLLLVIVAVVGILFIAFFYY
KDYASIGRNNSYLNKMINPAHAYNSYKYLKITYLTKELAYKKIGQDASLENSSNDKPTLMILVVGETARSQNIFYNGYAR
NTNPHTQDMGIISFQDVSSCGTATAHSLPCMFSNMNRSNYDKNRAITQDNALDVISHAGVDMLWIENDGGDKRVADNFSK
IEIAAEDSPELCDAGSCYDEVLLQNLDEKIQKNKGNQLIALHIKGSHGPTYWQRYPESKARFTPACNQSDIEHCSDQEIV
NVYDNTLVYTDYIIAQTIKKLTEYSKQYNVALMYISDHGESLGENGLYLHGAPYMIAPEEQTQVPWFMWLGNGYAAAKGI
DKQCLLDKANNDTFSHDNLFHTLLGFYGVDSTAKDSQLDIIASCKQSISNIAKTKSVDPEIN

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=503, Percent_Identity=39.5626242544732, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI87082286, Length=487, Percent_Identity=26.2833675564682, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082223, Length=457, Percent_Identity=24.72647702407, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790392, Length=522, Percent_Identity=22.7969348659004, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61247; Mature: 61247

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFLITLLSIYYGFVFNYPIIDKIYILSDNQSVFYFLSPLVLTFAFIIIFSILALPYLFK
CCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQIPLLITSALAFYASIKYNVMFDYSMIENIFETDTGEAFTYINFGSISYFIFLGLLPA
HHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHH
LIVFKTKITYAKSITREIINRLLLVIVAVVGILFIAFFYYKDYASIGRNNSYLNKMINPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHH
HAYNSYKYLKITYLTKELAYKKIGQDASLENSSNDKPTLMILVVGETARSQNIFYNGYAR
HHCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECEEC
NTNPHTQDMGIISFQDVSSCGTATAHSLPCMFSNMNRSNYDKNRAITQDNALDVISHAGV
CCCCCCCCCCEEEECCHHCCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCE
DMLWIENDGGDKRVADNFSKIEIAAEDSPELCDAGSCYDEVLLQNLDEKIQKNKGNQLIA
EEEEEECCCCCCHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
LHIKGSHGPTYWQRYPESKARFTPACNQSDIEHCSDQEIVNVYDNTLVYTDYIIAQTIKK
EEEECCCCCHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCEEEEHHHHHHHHHHH
LTEYSKQYNVALMYISDHGESLGENGLYLHGAPYMIAPEEQTQVPWFMWLGNGYAAAKGI
HHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCCCCCCCEEEEECCCCHHHCCC
DKQCLLDKANNDTFSHDNLFHTLLGFYGVDSTAKDSQLDIIASCKQSISNIAKTKSVDPE
CHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IN
CC
>Mature Secondary Structure
MVFLITLLSIYYGFVFNYPIIDKIYILSDNQSVFYFLSPLVLTFAFIIIFSILALPYLFK
CCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQIPLLITSALAFYASIKYNVMFDYSMIENIFETDTGEAFTYINFGSISYFIFLGLLPA
HHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHH
LIVFKTKITYAKSITREIINRLLLVIVAVVGILFIAFFYYKDYASIGRNNSYLNKMINPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHH
HAYNSYKYLKITYLTKELAYKKIGQDASLENSSNDKPTLMILVVGETARSQNIFYNGYAR
HHCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECEEC
NTNPHTQDMGIISFQDVSSCGTATAHSLPCMFSNMNRSNYDKNRAITQDNALDVISHAGV
CCCCCCCCCCEEEECCHHCCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCE
DMLWIENDGGDKRVADNFSKIEIAAEDSPELCDAGSCYDEVLLQNLDEKIQKNKGNQLIA
EEEEEECCCCCCHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
LHIKGSHGPTYWQRYPESKARFTPACNQSDIEHCSDQEIVNVYDNTLVYTDYIIAQTIKK
EEEECCCCCHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCEEEEHHHHHHHHHHH
LTEYSKQYNVALMYISDHGESLGENGLYLHGAPYMIAPEEQTQVPWFMWLGNGYAAAKGI
HHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCCCCCCCEEEEECCCCHHHCCC
DKQCLLDKANNDTFSHDNLFHTLLGFYGVDSTAKDSQLDIIASCKQSISNIAKTKSVDPE
CHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]