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The map label for this gene is iscS

Identifier: 119945068

GI number: 119945068

Start: 1643086

End: 1644297

Strand: Direct

Name: iscS

Synonym: Ping_1324

Alternate gene names: 119945068

Gene position: 1643086-1644297 (Clockwise)

Preceding gene: 119945067

Following gene: 119945069

Centisome position: 36.04

GC content: 42.08

Gene sequence:

>1212_bases
ATGAAATTACCAATTTACCTTGATTACTCTTCAACCACACCGGTTGACCCACGTGTTGCAGAAAAAATGATGCAATATCT
AACCCCAGGCGGCGAATTCGGTAATCCAGCTTCCCGTTCGCATCGTTTTGGTTGGCATGCCGAGGAAGCGGTAGATGTCG
CTCGTGAACAAATCGCCGATTTAATCAGTGGAGATCCTCGTGAAATTGTTTTCACCTCCGGCGCGACAGAATCAAACAAT
CTTGCTATTAAAGGTGCTGCGCATTTTTATGTTAAAAAAGGCAAGCACATTATTACCGCAAAAACTGAGCATAAAGCAGT
ATTAGACACTTGTCGTCAGCTTGAGCGTGAAGGTTACGAAGTGACTTACCTTGATCCTAAGGAAGATGGCATCGTAACAC
CACAACAATTAAAAGAGGTATTACGTCCAGACACTGTGCTGGTGAGTTTAATGCACGTTAATAATGAAATTGGCGTTATT
CAAGATATCGCAGGATTTGGCGAACTATGCCGTGCAAATAAAACTATCTTTCATGTTGATGCGTCGCAAAGTGTCGGCAA
AATAGAGATTGATACCCAAGCTTTAAAAGTAGACTTAATGTCATTTTCAGCGCATAAGATTTACGGTCCTAAGGGTATTG
GCGCACTATATGTCAGTCGTAAACCCCGCGTGCGTTTAGAAGCACAAATGCACGGTGGCGGACACGAACGTGGTATGCGC
AGTGGTACATTGCCAACACACCAAATAGCCGCTATGGGCGAAGCTTTCCGTATTGCCAAGGAAGAAATGGCTTCTGATGA
GGTACGTATTTTGGCATTAAGAACGCGTTTATTAAATGGCGTTAATGATATGGAAGAAGTGTATGTCAACGGCTCTCTAG
AAAACCGTATTGCGGGTAATATCAACATTAGTTTTAACTATGTTGAAGGTGAGTCTTTGGTGATGGCGCTTAAAGATTTA
GCGGTTTCTTCAGGCAGTGCATGCACCTCGGCAAGTTTAGAACCTTCTTATGTTTTACGTGCAATAGGCCGTGATGATGA
GTTGGCGCACAGTTCAATTCGTTTCAGTATTGGCCGTTTCACTACTGAAGAAGAAATTGATTACGCAATTAATATTATTC
GTAAAAATATTGGACGCTTACGTGATATGTCTCCTTTATGGGATATGTATAAAGAAGGCATCGATATTAAGAGTATCGAG
TGGTCACATTAA

Upstream 100 bases:

>100_bases
TACAGCATATTTCTGGAATTCAAAATAATAAATTTAACCAAACTGAAAAGCTAAATGTTAAGTCACACTAATACGTTTAT
ATAGCACAGCGGAGATACAA

Downstream 100 bases:

>100_bases
TAGGGTTGATTGAGCTTTTTAGATAATAGTACAAGGCTGATAAAAGCAGCATTGTATTTTTATCAAAGAGAACGAACACT
AAATTACATTCACAAACGAT

Product: cysteine desulfurase IscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKLPIYLDYSSTTPVDPRVAEKMMQYLTPGGEFGNPASRSHRFGWHAEEAVDVAREQIADLISGDPREIVFTSGATESNN
LAIKGAAHFYVKKGKHIITAKTEHKAVLDTCRQLEREGYEVTYLDPKEDGIVTPQQLKEVLRPDTVLVSLMHVNNEIGVI
QDIAGFGELCRANKTIFHVDASQSVGKIEIDTQALKVDLMSFSAHKIYGPKGIGALYVSRKPRVRLEAQMHGGGHERGMR
SGTLPTHQIAAMGEAFRIAKEEMASDEVRILALRTRLLNGVNDMEEVYVNGSLENRIAGNINISFNYVEGESLVMALKDL
AVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFSIGRFTTEEEIDYAINIIRKNIGRLRDMSPLWDMYKEGIDIKSIE
WSH

Sequences:

>Translated_403_residues
MKLPIYLDYSSTTPVDPRVAEKMMQYLTPGGEFGNPASRSHRFGWHAEEAVDVAREQIADLISGDPREIVFTSGATESNN
LAIKGAAHFYVKKGKHIITAKTEHKAVLDTCRQLEREGYEVTYLDPKEDGIVTPQQLKEVLRPDTVLVSLMHVNNEIGVI
QDIAGFGELCRANKTIFHVDASQSVGKIEIDTQALKVDLMSFSAHKIYGPKGIGALYVSRKPRVRLEAQMHGGGHERGMR
SGTLPTHQIAAMGEAFRIAKEEMASDEVRILALRTRLLNGVNDMEEVYVNGSLENRIAGNINISFNYVEGESLVMALKDL
AVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFSIGRFTTEEEIDYAINIIRKNIGRLRDMSPLWDMYKEGIDIKSIE
WSH
>Mature_403_residues
MKLPIYLDYSSTTPVDPRVAEKMMQYLTPGGEFGNPASRSHRFGWHAEEAVDVAREQIADLISGDPREIVFTSGATESNN
LAIKGAAHFYVKKGKHIITAKTEHKAVLDTCRQLEREGYEVTYLDPKEDGIVTPQQLKEVLRPDTVLVSLMHVNNEIGVI
QDIAGFGELCRANKTIFHVDASQSVGKIEIDTQALKVDLMSFSAHKIYGPKGIGALYVSRKPRVRLEAQMHGGGHERGMR
SGTLPTHQIAAMGEAFRIAKEEMASDEVRILALRTRLLNGVNDMEEVYVNGSLENRIAGNINISFNYVEGESLVMALKDL
AVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFSIGRFTTEEEIDYAINIIRKNIGRLRDMSPLWDMYKEGIDIKSIE
WSH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=401, Percent_Identity=57.85536159601, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=29.9043062200957, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI48994898, Length=403, Percent_Identity=76.9230769230769, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=180, Percent_Identity=31.6666666666667, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789175, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=402, Percent_Identity=57.9601990049751, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=32.0872274143302, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=55.6390977443609, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI20129463, Length=401, Percent_Identity=57.1072319201995, Blast_Score=480, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_PSYIN (A1SUI4)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942748.1
- ProteinModelPortal:   A1SUI4
- SMR:   A1SUI4
- STRING:   A1SUI4
- GeneID:   4626437
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1324
- eggNOG:   COG1104
- HOGENOM:   HBG635316
- OMA:   DMSPLWD
- PhylomeDB:   A1SUI4
- BioCyc:   PING357804:PING_1324-MONOMER
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44766; Mature: 44766

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSSTTPVDPRVAEKMMQYLTPGGEFGNPASRSHRFGWHAEEAVDVAREQIAD
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LISGDPREIVFTSGATESNNLAIKGAAHFYVKKGKHIITAKTEHKAVLDTCRQLEREGYE
HHCCCCCEEEEECCCCCCCCEEEEECEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCE
VTYLDPKEDGIVTPQQLKEVLRPDTVLVSLMHVNNEIGVIQDIAGFGELCRANKTIFHVD
EEEECCCCCCCCCHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
ASQSVGKIEIDTQALKVDLMSFSAHKIYGPKGIGALYVSRKPRVRLEAQMHGGGHERGMR
CCCCCCEEEECHHHEEEEEECCCCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHCCCC
SGTLPTHQIAAMGEAFRIAKEEMASDEVRILALRTRLLNGVNDMEEVYVNGSLENRIAGN
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHEEECCCCCCCEEEE
INISFNYVEGESLVMALKDLAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFSIGRF
EEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHEEEECCC
TTEEEIDYAINIIRKNIGRLRDMSPLWDMYKEGIDIKSIEWSH
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCC
>Mature Secondary Structure
MKLPIYLDYSSTTPVDPRVAEKMMQYLTPGGEFGNPASRSHRFGWHAEEAVDVAREQIAD
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LISGDPREIVFTSGATESNNLAIKGAAHFYVKKGKHIITAKTEHKAVLDTCRQLEREGYE
HHCCCCCEEEEECCCCCCCCEEEEECEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCE
VTYLDPKEDGIVTPQQLKEVLRPDTVLVSLMHVNNEIGVIQDIAGFGELCRANKTIFHVD
EEEECCCCCCCCCHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
ASQSVGKIEIDTQALKVDLMSFSAHKIYGPKGIGALYVSRKPRVRLEAQMHGGGHERGMR
CCCCCCEEEECHHHEEEEEECCCCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHCCCC
SGTLPTHQIAAMGEAFRIAKEEMASDEVRILALRTRLLNGVNDMEEVYVNGSLENRIAGN
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHEEECCCCCCCEEEE
INISFNYVEGESLVMALKDLAVSSGSACTSASLEPSYVLRAIGRDDELAHSSIRFSIGRF
EEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHEEEECCC
TTEEEIDYAINIIRKNIGRLRDMSPLWDMYKEGIDIKSIEWSH
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA