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The map label for this gene is trmJ [H]

Identifier: 119945066

GI number: 119945066

Start: 1641670

End: 1642434

Strand: Direct

Name: trmJ [H]

Synonym: Ping_1322

Alternate gene names: 119945066

Gene position: 1641670-1642434 (Clockwise)

Preceding gene: 119945062

Following gene: 119945067

Centisome position: 36.0

GC content: 41.05

Gene sequence:

>765_bases
ATGCTAGAAAACGTGCGCATTATACTCATTGGCACTTCTCATCCCGGTAATGTCGGCAGTGCAGCAAGAGCAATGAAAAC
AATGGGGTTAACAAAACTAGTACTTGTCACGCCTGAATGTGAAATTGATGGAAAATCCATTGCACTTTCTGCGGGTGCTT
CGGATGTATTAAAAAATCATAAAGTTTGCAATACGTTAGATGATGCAATTGCTGATTGTGGTTTAGTGATTGGTGCAAGT
GCTCGCTCACGTACTTTGGACTGGCCTATGTTGGATCCTCGAGAAATGGGTCAAAAAGTTGTCGAAGAAGGCAATAAACA
TCCTGTGGCATTAGTATTTGGTCGTGAAAATAGTGGTTTGACTAATGATGAGCTACAAAAGTGTCATTTTCATGTGTTTA
TTCCTGCAAATCCTGACTATAGCTCATTAAATTTAGCAATGGCTGTACAAACTCTGAGCTATGAAGTTCGCATGGAATTT
TTGGCATCTAAGGCTTACCCTGAGCAGCCACAGGAATATCCTCTTGCTGACGAAATCGAAGGATTGTTTAACCACCTAGA
ACAAACTTTAAAAGATACCGGCTTTATCGTTCCCGCACATCCGGGATTGGTAATGAATAAATTACGCCGCTTTTTCAACC
GCGCTCGTCCTGAAGTCAAAGAGCTGAGAATGTGGCGGGGGATTTTAAGTAGCGTCCAAAAATCAGTGAGTTCAGCAGAA
AAAGAAGTTAATCAATCGATGCAGGCAAAGAATAAAATCTCATAA

Upstream 100 bases:

>100_bases
AAAATAGATAATTAAACAGCGTTAATAGAACTATTTTTTGTAATTTAATTAGTGGTAAACTTCGGGCACTTAAATTCGTC
TTAAACATAAAGGAATACAA

Downstream 100 bases:

>100_bases
AGTGCATTATTTTTACCTGACTATTTTTGTCAGGTATTTTTACTTGACTATTTTTGTCAGGTATTTATAATAACACAATG
AAGTCCACGGAAAGTGTTGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLENVRIILIGTSHPGNVGSAARAMKTMGLTKLVLVTPECEIDGKSIALSAGASDVLKNHKVCNTLDDAIADCGLVIGAS
ARSRTLDWPMLDPREMGQKVVEEGNKHPVALVFGRENSGLTNDELQKCHFHVFIPANPDYSSLNLAMAVQTLSYEVRMEF
LASKAYPEQPQEYPLADEIEGLFNHLEQTLKDTGFIVPAHPGLVMNKLRRFFNRARPEVKELRMWRGILSSVQKSVSSAE
KEVNQSMQAKNKIS

Sequences:

>Translated_254_residues
MLENVRIILIGTSHPGNVGSAARAMKTMGLTKLVLVTPECEIDGKSIALSAGASDVLKNHKVCNTLDDAIADCGLVIGAS
ARSRTLDWPMLDPREMGQKVVEEGNKHPVALVFGRENSGLTNDELQKCHFHVFIPANPDYSSLNLAMAVQTLSYEVRMEF
LASKAYPEQPQEYPLADEIEGLFNHLEQTLKDTGFIVPAHPGLVMNKLRRFFNRARPEVKELRMWRGILSSVQKSVSSAE
KEVNQSMQAKNKIS
>Mature_254_residues
MLENVRIILIGTSHPGNVGSAARAMKTMGLTKLVLVTPECEIDGKSIALSAGASDVLKNHKVCNTLDDAIADCGLVIGAS
ARSRTLDWPMLDPREMGQKVVEEGNKHPVALVFGRENSGLTNDELQKCHFHVFIPANPDYSSLNLAMAVQTLSYEVRMEF
LASKAYPEQPQEYPLADEIEGLFNHLEQTLKDTGFIVPAHPGLVMNKLRRFFNRARPEVKELRMWRGILSSVQKSVSSAE
KEVNQSMQAKNKIS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=62.6050420168067, Blast_Score=306, Evalue=7e-85,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=35.9477124183007, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28073; Mature: 28073

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENVRIILIGTSHPGNVGSAARAMKTMGLTKLVLVTPECEIDGKSIALSAGASDVLKNH
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCHHHHHHCC
KVCNTLDDAIADCGLVIGASARSRTLDWPMLDPREMGQKVVEEGNKHPVALVFGRENSGL
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCC
TNDELQKCHFHVFIPANPDYSSLNLAMAVQTLSYEVRMEFLASKAYPEQPQEYPLADEIE
CHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
GLFNHLEQTLKDTGFIVPAHPGLVMNKLRRFFNRARPEVKELRMWRGILSSVQKSVSSAE
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KEVNQSMQAKNKIS
HHHHHHHHHHHCCC
>Mature Secondary Structure
MLENVRIILIGTSHPGNVGSAARAMKTMGLTKLVLVTPECEIDGKSIALSAGASDVLKNH
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCHHHHHHCC
KVCNTLDDAIADCGLVIGASARSRTLDWPMLDPREMGQKVVEEGNKHPVALVFGRENSGL
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCC
TNDELQKCHFHVFIPANPDYSSLNLAMAVQTLSYEVRMEFLASKAYPEQPQEYPLADEIE
CHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
GLFNHLEQTLKDTGFIVPAHPGLVMNKLRRFFNRARPEVKELRMWRGILSSVQKSVSSAE
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KEVNQSMQAKNKIS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA