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The map label for this gene is ycdT [H]

Identifier: 119945057

GI number: 119945057

Start: 1630651

End: 1632282

Strand: Direct

Name: ycdT [H]

Synonym: Ping_1313

Alternate gene names: 119945057

Gene position: 1630651-1632282 (Clockwise)

Preceding gene: 119945056

Following gene: 119945059

Centisome position: 35.76

GC content: 42.34

Gene sequence:

>1632_bases
ATGAGCTACATATACAGCATTAAAGGAAAATTGCTTTTGGTCTTTATCTCTGCAGTCCTGGTATTTAGCAGCGATGACTT
GCTAAAGATCTATTTTATTAAGCAGTTCACCTTCTCGATAGAAAAATTCGTTTCAACCGCAGTACCTATGATAAACGCGG
CCAACGAACTTGAAGTTATTGGGAATAGTATCGCGCTACAAACCAATCGTCTTATCGGGGCCCAACAACAAAAAAAATTG
ACGGATTCTTATATATCGATTGGCAAATTATTAGACGAATTGGAAATAAAAACCTCTGCCATCTCACTGGAAGGGGGAGA
TATTAATGTGCTAACAGTGAACAGTCTGAGCCAGTCAATCCGCTCCCTGTCACAAATTGTGTTTCAAGTTATCAATAGTA
GTTTTACAAAAGAAGATCAGGCGCGGAGTGTAGCTGCTTCGGAATTAGAGATTTTAGAGACTATACGCAACGACGTTCTT
GATGCTTACACACAACAAACTGCGACAGTGATAGATACTGCTCTTTTTGCGAGTATCCAAGACATCACCATCTGTATGGT
CGTTTCGATCGAACGCGCCCTTTATGCTGAAACGTTACAACAGCTAAAAAAACAAGAAGATGAATTCCTTCTTTGCAGTG
AAAAGCTAATGGCTGAAGTGAGCGAAAATAAGTCTTCGTTGGCACACTCCTCCACCATCGTGCAATGGCTGAATACGCCT
TCAGAACAAAGGAACAGAGTTTTTGAAATTAAACAGCGCGTATTGTTCTTGCAGGTGTCTGCTAAAAAATATCTAACAGA
ATTGGATCAACTTGCCGATCAGCTAACATCACTCACGTCAGATTATAGACAACGAATTTTTCGTCGCTTTCAAACCACTG
CCGATGCCGCAATCGTTCAAGAAAAAAGAGCCATCAATCTCACCGTGATCCTTAATATTATTGCCGCTACACTGCTGTTA
ATTTTTTACTGGATAATTGTCGTACGCGGCTTTGGTAACCGTCTGACGATAATCAGTCAGGCCATGCGTAGTATCCCTAC
CAGCGATTCAGATACCAAAATTCGGGTTCGGGGGCGTGATGAAATTGCTGCCATGGCACGCGCTTTAGAGCGATTACTAA
AGGAGGCTTTGCTGACACATCAGATGGCAACCATTGACCACCTAACACAGGTAAACAATCGGGCCAGCTTTTTTACTTAT
GCGGAAAAAGAGACTGAGCGCTCCAAAAGACGACCAAATAAAACCTGCATCATGATGATAGATATCGATCATTTTAAACA
TATCAATGACACCTACGGCCATGATGTTGGAGATAAACTTTTGTCCTTGGTGGCGGATAACTGCCTAAAGTGTACAAGAA
CTATTGATGTATTTGCCCGCCTGGGGGGTGACGAATTCACCTTGATGATGCCGGACACTGATCTTGTATCAGGAAGACTG
GCGGCAGAACGATTACGACAACGCATCGATGAGTTACAGCTGGAGCTTACACCTGACGTTATAATAAAAGTGACCACAAG
TATTGGGATATCTGTGGTCAAGCTAAATGAAACCCCCATTGAAACCGCATTAATTCAGGCCGATAAAGCACTCTATCTGG
CCAAAACTGGTGGACGGAATCAAAGTAAATAG

Upstream 100 bases:

>100_bases
ACTGACTCCTTTTGATCCAGATAACAACTACCGTCGATTTTATACAAAAATCTGGTTGGGTAAATAAGTGACTTTAATAA
CATGTGTAGCAGGAAACTGA

Downstream 100 bases:

>100_bases
CTGGACAGCCAAATTTTTTGAGTGTAGCGGGTTTAGAGCGTGAGGCTTGGTTAGCATTAGCATGCTATTTTGGCGAAAGT
ATCATGGCGCCGTGGGCTTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSYIYSIKGKLLLVFISAVLVFSSDDLLKIYFIKQFTFSIEKFVSTAVPMINAANELEVIGNSIALQTNRLIGAQQQKKL
TDSYISIGKLLDELEIKTSAISLEGGDINVLTVNSLSQSIRSLSQIVFQVINSSFTKEDQARSVAASELEILETIRNDVL
DAYTQQTATVIDTALFASIQDITICMVVSIERALYAETLQQLKKQEDEFLLCSEKLMAEVSENKSSLAHSSTIVQWLNTP
SEQRNRVFEIKQRVLFLQVSAKKYLTELDQLADQLTSLTSDYRQRIFRRFQTTADAAIVQEKRAINLTVILNIIAATLLL
IFYWIIVVRGFGNRLTIISQAMRSIPTSDSDTKIRVRGRDEIAAMARALERLLKEALLTHQMATIDHLTQVNNRASFFTY
AEKETERSKRRPNKTCIMMIDIDHFKHINDTYGHDVGDKLLSLVADNCLKCTRTIDVFARLGGDEFTLMMPDTDLVSGRL
AAERLRQRIDELQLELTPDVIIKVTTSIGISVVKLNETPIETALIQADKALYLAKTGGRNQSK

Sequences:

>Translated_543_residues
MSYIYSIKGKLLLVFISAVLVFSSDDLLKIYFIKQFTFSIEKFVSTAVPMINAANELEVIGNSIALQTNRLIGAQQQKKL
TDSYISIGKLLDELEIKTSAISLEGGDINVLTVNSLSQSIRSLSQIVFQVINSSFTKEDQARSVAASELEILETIRNDVL
DAYTQQTATVIDTALFASIQDITICMVVSIERALYAETLQQLKKQEDEFLLCSEKLMAEVSENKSSLAHSSTIVQWLNTP
SEQRNRVFEIKQRVLFLQVSAKKYLTELDQLADQLTSLTSDYRQRIFRRFQTTADAAIVQEKRAINLTVILNIIAATLLL
IFYWIIVVRGFGNRLTIISQAMRSIPTSDSDTKIRVRGRDEIAAMARALERLLKEALLTHQMATIDHLTQVNNRASFFTY
AEKETERSKRRPNKTCIMMIDIDHFKHINDTYGHDVGDKLLSLVADNCLKCTRTIDVFARLGGDEFTLMMPDTDLVSGRL
AAERLRQRIDELQLELTPDVIIKVTTSIGISVVKLNETPIETALIQADKALYLAKTGGRNQSK
>Mature_542_residues
SYIYSIKGKLLLVFISAVLVFSSDDLLKIYFIKQFTFSIEKFVSTAVPMINAANELEVIGNSIALQTNRLIGAQQQKKLT
DSYISIGKLLDELEIKTSAISLEGGDINVLTVNSLSQSIRSLSQIVFQVINSSFTKEDQARSVAASELEILETIRNDVLD
AYTQQTATVIDTALFASIQDITICMVVSIERALYAETLQQLKKQEDEFLLCSEKLMAEVSENKSSLAHSSTIVQWLNTPS
EQRNRVFEIKQRVLFLQVSAKKYLTELDQLADQLTSLTSDYRQRIFRRFQTTADAAIVQEKRAINLTVILNIIAATLLLI
FYWIIVVRGFGNRLTIISQAMRSIPTSDSDTKIRVRGRDEIAAMARALERLLKEALLTHQMATIDHLTQVNNRASFFTYA
EKETERSKRRPNKTCIMMIDIDHFKHINDTYGHDVGDKLLSLVADNCLKCTRTIDVFARLGGDEFTLMMPDTDLVSGRLA
AERLRQRIDELQLELTPDVIIKVTTSIGISVVKLNETPIETALIQADKALYLAKTGGRNQSK

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=37.9518072289157, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=36.4197530864198, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=33.125, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=36.8098159509202, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=34.4444444444444, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=34.1614906832298, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=32.7586206896552, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=219, Percent_Identity=29.2237442922374, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787816, Length=131, Percent_Identity=32.0610687022901, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=153, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=29.9363057324841, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61113; Mature: 60982

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIYSIKGKLLLVFISAVLVFSSDDLLKIYFIKQFTFSIEKFVSTAVPMINAANELEVI
CCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GNSIALQTNRLIGAQQQKKLTDSYISIGKLLDELEIKTSAISLEGGDINVLTVNSLSQSI
CCHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHH
RSLSQIVFQVINSSFTKEDQARSVAASELEILETIRNDVLDAYTQQTATVIDTALFASIQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DITICMVVSIERALYAETLQQLKKQEDEFLLCSEKLMAEVSENKSSLAHSSTIVQWLNTP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SEQRNRVFEIKQRVLFLQVSAKKYLTELDQLADQLTSLTSDYRQRIFRRFQTTADAAIVQ
CHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKRAINLTVILNIIAATLLLIFYWIIVVRGFGNRLTIISQAMRSIPTSDSDTKIRVRGRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEECCHH
EIAAMARALERLLKEALLTHQMATIDHLTQVNNRASFFTYAEKETERSKRRPNKTCIMMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCEEEEEE
DIDHFKHINDTYGHDVGDKLLSLVADNCLKCTRTIDVFARLGGDEFTLMMPDTDLVSGRL
ECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
AAERLRQRIDELQLELTPDVIIKVTTSIGISVVKLNETPIETALIQADKALYLAKTGGRN
HHHHHHHHHHHHHEECCCCEEEEEECCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCC
QSK
CCC
>Mature Secondary Structure 
SYIYSIKGKLLLVFISAVLVFSSDDLLKIYFIKQFTFSIEKFVSTAVPMINAANELEVI
CEEEECCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GNSIALQTNRLIGAQQQKKLTDSYISIGKLLDELEIKTSAISLEGGDINVLTVNSLSQSI
CCHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHH
RSLSQIVFQVINSSFTKEDQARSVAASELEILETIRNDVLDAYTQQTATVIDTALFASIQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DITICMVVSIERALYAETLQQLKKQEDEFLLCSEKLMAEVSENKSSLAHSSTIVQWLNTP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SEQRNRVFEIKQRVLFLQVSAKKYLTELDQLADQLTSLTSDYRQRIFRRFQTTADAAIVQ
CHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKRAINLTVILNIIAATLLLIFYWIIVVRGFGNRLTIISQAMRSIPTSDSDTKIRVRGRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEECCHH
EIAAMARALERLLKEALLTHQMATIDHLTQVNNRASFFTYAEKETERSKRRPNKTCIMMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCEEEEEE
DIDHFKHINDTYGHDVGDKLLSLVADNCLKCTRTIDVFARLGGDEFTLMMPDTDLVSGRL
ECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
AAERLRQRIDELQLELTPDVIIKVTTSIGISVVKLNETPIETALIQADKALYLAKTGGRN
HHHHHHHHHHHHHEECCCCEEEEEECCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCC
QSK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]