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The map label for this gene is rsmE [H]

Identifier: 119945034

GI number: 119945034

Start: 1593516

End: 1594244

Strand: Reverse

Name: rsmE [H]

Synonym: Ping_1288

Alternate gene names: 119945034

Gene position: 1594244-1593516 (Counterclockwise)

Preceding gene: 119945040

Following gene: 119945033

Centisome position: 34.96

GC content: 43.62

Gene sequence:

>729_bases
ATGCGAATTCCTCGTTTTTATGATCCACAAACATTAACCGTTGCCACTGAAGTCAATCTTTCAAAAGATGCGATCCAACA
TATCCATGTTTTACGCTTAAGACCTGGCGCACAAATAATTCTGTTTAATGGCCTTGGAGGAGAGGTTCAGGCGACTTTGA
CTAGTGCGCAGAAACGCAGTTGCACGGCGTTGATAAATTCCTTTAATCAAACGGATAATGAAAGTCCATTACATCTGCAC
TTAGGGCAAGTCATTTCGCGTGGCGATCGCATGGAGTTTGTTATTCAAAAAGCGGTAGAGTTAGGTGTTAATGAAATAAC
ACCGTTATTAAGCGAGCGTTGTGGTGTTAAACTCGATGATCAGCGCATGGCTAAAAAACAGCAGCAGTGGCAAAAAATAG
CGCTAGCTGCCTGCGAGCAATCAGGTCGTAATATCATTCCGGTGATTCATGAAACCGAAGCATTAACAACTTGGTGCGAA
AGTGCGGAGCAGGCCACCAAACTGACACTGCATCCACGCGCTAAATATTCAATTAATACCTTACCGCCGGAAATCAAACG
TATTCGTTTACTGATCGGCCCAGAAGGTGGTTTAAGCGATCAAGAAGTAACCTTGACCGAAACATTAAACTTCACAGAAA
CATTATTAGGCCCGCGTATTTTACGCACAGAAACCGCTGCACTCAGTGCAATTACTGCATTACAATGTCGTTTTGGCGAC
CTTGCATAG

Upstream 100 bases:

>100_bases
TTTATCGGAGTTGATTAGATAAAAAAATTTTTAGCATAGTTTTATCGCCTCTTTTCACGTTATACTTGGTACTATTTCCC
GCTACCTAAACATAATAATT

Downstream 100 bases:

>100_bases
AATGCTCAGCAAGACTGATAAATAGGAAATAAAATGACCATTAAAATTGGGATCGTAATGGATCCAATCAAAGATATTAA
AATTCATAAAGACTCTAGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRIPRFYDPQTLTVATEVNLSKDAIQHIHVLRLRPGAQIILFNGLGGEVQATLTSAQKRSCTALINSFNQTDNESPLHLH
LGQVISRGDRMEFVIQKAVELGVNEITPLLSERCGVKLDDQRMAKKQQQWQKIALAACEQSGRNIIPVIHETEALTTWCE
SAEQATKLTLHPRAKYSINTLPPEIKRIRLLIGPEGGLSDQEVTLTETLNFTETLLGPRILRTETAALSAITALQCRFGD
LA

Sequences:

>Translated_242_residues
MRIPRFYDPQTLTVATEVNLSKDAIQHIHVLRLRPGAQIILFNGLGGEVQATLTSAQKRSCTALINSFNQTDNESPLHLH
LGQVISRGDRMEFVIQKAVELGVNEITPLLSERCGVKLDDQRMAKKQQQWQKIALAACEQSGRNIIPVIHETEALTTWCE
SAEQATKLTLHPRAKYSINTLPPEIKRIRLLIGPEGGLSDQEVTLTETLNFTETLLGPRILRTETAALSAITALQCRFGD
LA
>Mature_242_residues
MRIPRFYDPQTLTVATEVNLSKDAIQHIHVLRLRPGAQIILFNGLGGEVQATLTSAQKRSCTALINSFNQTDNESPLHLH
LGQVISRGDRMEFVIQKAVELGVNEITPLLSERCGVKLDDQRMAKKQQQWQKIALAACEQSGRNIIPVIHETEALTTWCE
SAEQATKLTLHPRAKYSINTLPPEIKRIRLLIGPEGGLSDQEVTLTETLNFTETLLGPRILRTETAALSAITALQCRFGD
LA

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=57.6131687242798, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26869; Mature: 26869

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRFYDPQTLTVATEVNLSKDAIQHIHVLRLRPGAQIILFNGLGGEVQATLTSAQKRS
CCCCCCCCCCEEEEEEECCCCHHHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHH
CTALINSFNQTDNESPLHLHLGQVISRGDRMEFVIQKAVELGVNEITPLLSERCGVKLDD
HHHHHHHHCCCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCH
QRMAKKQQQWQKIALAACEQSGRNIIPVIHETEALTTWCESAEQATKLTLHPRAKYSINT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHEEEECCCCCEECCC
LPPEIKRIRLLIGPEGGLSDQEVTLTETLNFTETLLGPRILRTETAALSAITALQCRFGD
CCCCCEEEEEEECCCCCCCCCEEEEEHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LA
CC
>Mature Secondary Structure
MRIPRFYDPQTLTVATEVNLSKDAIQHIHVLRLRPGAQIILFNGLGGEVQATLTSAQKRS
CCCCCCCCCCEEEEEEECCCCHHHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHH
CTALINSFNQTDNESPLHLHLGQVISRGDRMEFVIQKAVELGVNEITPLLSERCGVKLDD
HHHHHHHHCCCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCH
QRMAKKQQQWQKIALAACEQSGRNIIPVIHETEALTTWCESAEQATKLTLHPRAKYSINT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHEEEECCCCCEECCC
LPPEIKRIRLLIGPEGGLSDQEVTLTETLNFTETLLGPRILRTETAALSAITALQCRFGD
CCCCCEEEEEEECCCCCCCCCEEEEEHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]