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The map label for this gene is grcA

Identifier: 119945027

GI number: 119945027

Start: 1588287

End: 1588664

Strand: Reverse

Name: grcA

Synonym: Ping_1281

Alternate gene names: 119945027

Gene position: 1588664-1588287 (Counterclockwise)

Preceding gene: 119945028

Following gene: 119945025

Centisome position: 34.84

GC content: 42.59

Gene sequence:

>378_bases
ATGATCCAAGGCATTCAAATTACAAAATCAGATAACGAGAACTTATTAAACTCTATCTGGCTGATAGATAACGAAAAGAA
TGAAGCTCGCTGTTTAGTTGCTCCTGCTCAATACAAAGAAGATCAGGTTGTTCCTGCTTCTGAGCTAGGTGAATACGAGT
CTCGTGAAGTGGCTATCGAAGCGCCTAAAGCACAGGAAGGCGGTCAACACCTGAACGTAAACGTTCTGAAAAGAGAGACG
TTAGAAGATGCTATTAAGCACCCAGAAAACTACCCTCAGTTGACTATTCGTGTATCGGGTTATGCTGTACGCTTCAACTC
TCTGACGCCAGAGCAACAGCAAGACGTATTAACACGTACCTTTACAGAAAGCCTTTAA

Upstream 100 bases:

>100_bases
TAAAACCATAAGGTAAGCACATGTTTTTTGTTAGTCATCGTTTTCAAGATATAAGCATGTTAAGTCATAACAAATCAAAC
CACAAATAGGAGAGAAAATC

Downstream 100 bases:

>100_bases
TAGGTTTACTTAAGTTAATACCAATAAAAGCTGAATAAATTATTAATGCCGATCAGTTAATGTGAACGGCATTTTTTAAA
GCGAATACCCATTTTTAGAA

Product: autonomous glycyl radical cofactor GrcA

Products: CoA; pyruvate

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MIQGIQITKSDNENLLNSIWLIDNEKNEARCLVAPAQYKEDQVVPASELGEYESREVAIEAPKAQEGGQHLNVNVLKRET
LEDAIKHPENYPQLTIRVSGYAVRFNSLTPEQQQDVLTRTFTESL

Sequences:

>Translated_125_residues
MIQGIQITKSDNENLLNSIWLIDNEKNEARCLVAPAQYKEDQVVPASELGEYESREVAIEAPKAQEGGQHLNVNVLKRET
LEDAIKHPENYPQLTIRVSGYAVRFNSLTPEQQQDVLTRTFTESL
>Mature_125_residues
MIQGIQITKSDNENLLNSIWLIDNEKNEARCLVAPAQYKEDQVVPASELGEYESREVAIEAPKAQEGGQHLNVNVLKRET
LEDAIKHPENYPQLTIRVSGYAVRFNSLTPEQQQDVLTRTFTESL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=73.2283464566929, Blast_Score=192, Evalue=5e-51,
Organism=Escherichia coli, GI1787131, Length=60, Percent_Identity=80, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI48994926, Length=60, Percent_Identity=78.3333333333333, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1787044, Length=53, Percent_Identity=47.1698113207547, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRCA_PSYIN (A1SUE3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942707.1
- ProteinModelPortal:   A1SUE3
- SMR:   A1SUE3
- STRING:   A1SUE3
- GeneID:   4626759
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1281
- eggNOG:   COG3445
- HOGENOM:   HBG297207
- OMA:   GQFEYRE
- PhylomeDB:   A1SUE3
- ProtClustDB:   PRK11127
- BioCyc:   PING357804:PING_1281-MONOMER
- HAMAP:   MF_00806
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140
- PIRSF:   PIRSF000378

Pfam domain/function: PF01228 Gly_radical

EC number: 2.3.1.54

Molecular weight: Translated: 14167; Mature: 14167

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGIQITKSDNENLLNSIWLIDNEKNEARCLVAPAQYKEDQVVPASELGEYESREVAIE
CCCCEEEECCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCEEEEE
APKAQEGGQHLNVNVLKRETLEDAIKHPENYPQLTIRVSGYAVRFNSLTPEQQQDVLTRT
CCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTESL
HHHCC
>Mature Secondary Structure
MIQGIQITKSDNENLLNSIWLIDNEKNEARCLVAPAQYKEDQVVPASELGEYESREVAIE
CCCCEEEECCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCEEEEE
APKAQEGGQHLNVNVLKRETLEDAIKHPENYPQLTIRVSGYAVRFNSLTPEQQQDVLTRT
CCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTESL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA