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The map label for this gene is nspS [H]

Identifier: 119944987

GI number: 119944987

Start: 1544631

End: 1545725

Strand: Direct

Name: nspS [H]

Synonym: Ping_1238

Alternate gene names: 119944987

Gene position: 1544631-1545725 (Clockwise)

Preceding gene: 119944986

Following gene: 119944988

Centisome position: 33.88

GC content: 37.99

Gene sequence:

>1095_bases
ATGATAAAAAAATTAATAGAGAGGTCTTACCTTAAACAGCCTGTTTTTAACAGTTTGTTATCTGTTAAAAACAGGCTGGT
GGTTTTATGTTTATGTTTTTTTGTTGTGCCCCTTTATGGGCAGGAGATAAAAATATTAACCTGGGGAGACTACCTTTCAG
AATCCGTCGTTAAGACGTTTACAAAAAAAACGGGTCATACTGTTAAGCTTTATTATTATGATAGCGGGCCTGAGCTTAGT
GCCATGTTAACTAATGGTCAGGGCAGTCAATTTGATCTGGTACTGGCGGATAATGAAGTAACTGGACACTACGGAAAAGA
GGGACTATTTCAATCTCTAACGTCTATTTCCATCGATAACGTGCAATATAATAATTTGCAATCGCAACAAGCCTGCAGTC
AATACGGTATTCCCTATGCACAGGGCACAATGGGTATTATTTATCGTAGCTCAATATCTAAAACAGTTATCGATTCCTGG
CAAAATCTTTTATCGCCTCCAAAGGAACATGTGGGGACCACAATGATGTTTAAGGATGATATTGATACTATTGCCATTGC
TTTGCTTGCTCAGGGATTAGATCCCTTCTCTGTCGATGAAGATGAATTAAAATCTGCTTATTTATTACTGAGTGCACAAA
CAAAAGATCTGTTGCGCTATGGTTATTCTGTTAGTTATGTTTTACGGCAAGGAAAATCGTCACAACTCACTCTTGCGCCA
AGCTATTCAGATGATTTAAAAACGATAAAAAGGGCTTCCAGTCAAAATGATTGGAAATATGTTGTTCCAAAAGAGGGAAC
ACTTTTTTTTGTAGATTGTTTTACGGCCCCATCGGATGCTGTTGTAAAAGAAGGGACGAAAGCATTTTTGGCGTTTATTA
ATGATCCAGTCATGGCCTCTAAAAACGCGCAGGATATCGGGTTTTATACCACTAATGAAGCCGCTCTGTTAATCTCAAGT
GATGAGTATAAAAATGACCGGGAAATTTCACCGAGTGCAGAGATTCTAAAGCGTAGTTATAAATATAAACGCCTCCCTGA
AAGCGATGTTATTATCCGTAACAAAATGGTCACATTATTACAGACTCAAGAGTAG

Upstream 100 bases:

>100_bases
GGCCTGTTAAAACCATTTTTAATTAAGCTAGTTGGTTAGATTTTGGCATTGTTAGGTGTGTATTAGCACGTCTAATTAAC
CTTTTGGTGTGATCCTTATA

Downstream 100 bases:

>100_bases
CTGAATTATGACGCTTAGAAAAAAAATAAGGTATATTATATTGCCGGTGATCATCGCTCTTTCTGCCATGATTTCTTTGC
TTTATTATCATCTTTATAAG

Product: spermidine/putrescine-binding periplasmic protein-like protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: Norspermidine-binding protein [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MIKKLIERSYLKQPVFNSLLSVKNRLVVLCLCFFVVPLYGQEIKILTWGDYLSESVVKTFTKKTGHTVKLYYYDSGPELS
AMLTNGQGSQFDLVLADNEVTGHYGKEGLFQSLTSISIDNVQYNNLQSQQACSQYGIPYAQGTMGIIYRSSISKTVIDSW
QNLLSPPKEHVGTTMMFKDDIDTIAIALLAQGLDPFSVDEDELKSAYLLLSAQTKDLLRYGYSVSYVLRQGKSSQLTLAP
SYSDDLKTIKRASSQNDWKYVVPKEGTLFFVDCFTAPSDAVVKEGTKAFLAFINDPVMASKNAQDIGFYTTNEAALLISS
DEYKNDREISPSAEILKRSYKYKRLPESDVIIRNKMVTLLQTQE

Sequences:

>Translated_364_residues
MIKKLIERSYLKQPVFNSLLSVKNRLVVLCLCFFVVPLYGQEIKILTWGDYLSESVVKTFTKKTGHTVKLYYYDSGPELS
AMLTNGQGSQFDLVLADNEVTGHYGKEGLFQSLTSISIDNVQYNNLQSQQACSQYGIPYAQGTMGIIYRSSISKTVIDSW
QNLLSPPKEHVGTTMMFKDDIDTIAIALLAQGLDPFSVDEDELKSAYLLLSAQTKDLLRYGYSVSYVLRQGKSSQLTLAP
SYSDDLKTIKRASSQNDWKYVVPKEGTLFFVDCFTAPSDAVVKEGTKAFLAFINDPVMASKNAQDIGFYTTNEAALLISS
DEYKNDREISPSAEILKRSYKYKRLPESDVIIRNKMVTLLQTQE
>Mature_364_residues
MIKKLIERSYLKQPVFNSLLSVKNRLVVLCLCFFVVPLYGQEIKILTWGDYLSESVVKTFTKKTGHTVKLYYYDSGPELS
AMLTNGQGSQFDLVLADNEVTGHYGKEGLFQSLTSISIDNVQYNNLQSQQACSQYGIPYAQGTMGIIYRSSISKTVIDSW
QNLLSPPKEHVGTTMMFKDDIDTIAIALLAQGLDPFSVDEDELKSAYLLLSAQTKDLLRYGYSVSYVLRQGKSSQLTLAP
SYSDDLKTIKRASSQNDWKYVVPKEGTLFFVDCFTAPSDAVVKEGTKAFLAFINDPVMASKNAQDIGFYTTNEAALLISS
DEYKNDREISPSAEILKRSYKYKRLPESDVIIRNKMVTLLQTQE

Specific function: Acts as a sensor of norspermidine and enhances biofilm formation. When complexed to norspermidine, could interact with the periplasmic portion of mbaA to regulate its enzymatic activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=300, Percent_Identity=25.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787078, Length=325, Percent_Identity=24, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40882; Mature: 40882

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLIERSYLKQPVFNSLLSVKNRLVVLCLCFFVVPLYGQEIKILTWGDYLSESVVKTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
TKKTGHTVKLYYYDSGPELSAMLTNGQGSQFDLVLADNEVTGHYGKEGLFQSLTSISIDN
HHCCCCEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCEECC
VQYNNLQSQQACSQYGIPYAQGTMGIIYRSSISKTVIDSWQNLLSPPKEHVGTTMMFKDD
EECCCCCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHCCEEEEECC
IDTIAIALLAQGLDPFSVDEDELKSAYLLLSAQTKDLLRYGYSVSYVLRQGKSSQLTLAP
HHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEHHHHHHHHHCCHHEEHHHCCCCCEEEECC
SYSDDLKTIKRASSQNDWKYVVPKEGTLFFVDCFTAPSDAVVKEGTKAFLAFINDPVMAS
CCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCC
KNAQDIGFYTTNEAALLISSDEYKNDREISPSAEILKRSYKYKRLPESDVIIRNKMVTLL
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEHHHEEEEE
QTQE
ECCC
>Mature Secondary Structure
MIKKLIERSYLKQPVFNSLLSVKNRLVVLCLCFFVVPLYGQEIKILTWGDYLSESVVKTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
TKKTGHTVKLYYYDSGPELSAMLTNGQGSQFDLVLADNEVTGHYGKEGLFQSLTSISIDN
HHCCCCEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCEECC
VQYNNLQSQQACSQYGIPYAQGTMGIIYRSSISKTVIDSWQNLLSPPKEHVGTTMMFKDD
EECCCCCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHCCEEEEECC
IDTIAIALLAQGLDPFSVDEDELKSAYLLLSAQTKDLLRYGYSVSYVLRQGKSSQLTLAP
HHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEHHHHHHHHHCCHHEEHHHCCCCCEEEECC
SYSDDLKTIKRASSQNDWKYVVPKEGTLFFVDCFTAPSDAVVKEGTKAFLAFINDPVMAS
CCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCC
KNAQDIGFYTTNEAALLISSDEYKNDREISPSAEILKRSYKYKRLPESDVIIRNKMVTLL
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEHHHEEEEE
QTQE
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]