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The map label for this gene is gsp [H]

Identifier: 119944984

GI number: 119944984

Start: 1539855

End: 1541744

Strand: Direct

Name: gsp [H]

Synonym: Ping_1235

Alternate gene names: 119944984

Gene position: 1539855-1541744 (Clockwise)

Preceding gene: 119944983

Following gene: 119944985

Centisome position: 33.77

GC content: 43.6

Gene sequence:

>1890_bases
TTGAATTATAAATCCCCTTCTGTATTTGGTACGTTACTCGGTTATGCGCCAGGTGGTGTTGCTGCCTACTCCTCTGATTA
TGATACAGCCAGCGATATAAAATACCCCAAACGAAGCGCCTTTCGCAGTTATTTTAATGGTATTTATATGGGCTATAAAT
GGCAGTGTGTTGAATTTGCCCGGCGTTGGATGTATATCAATAAGGGGACTCTTTTTGATGATGTGGCCATGGCCTATGAG
ATATTTGACTTACGTACGGTGAGAAATCTTGCAACTAATAGCCTGCTGCCCTTACAGGCATTTTGTAATGGCAGTAAAAG
CTTGCCGCAGCCGGGCAGTTTGTTAATTTGGGATCAGGGGGGAGAGTTCGCAGAAACCGGTCATGTTGCCATTGTTACCG
AGGTATTTCCCGATAAATTACGTATTGTGGAGCAAAATTTAGATCACCAAATTTGGCCTGAGGGGCACTCTTTTAGCCGA
GAACTTCCCGCGACGACTACCGAAGAAGGTGAGTATTGGTTAAAATGCTCCTTTAATGATGCCACTATTTTAGGCTGGAT
GACACAAACCGAGGATGATCGCGATGCCGAGCCGACTCTAGAAACCAATAAGCAGTTATTTAATTTAATCAGCCGCCGGG
CAAAAGAGCAGCCCAACCCGCAAAAATCTTGGCTTAATGTTGCTAATGAGGATGAGGCCGCTTATGTGAAGGCGATGCAT
GGCCATAAACTTAGCGCGGTTACAGCGGATCAATATCGTTATTATGTTTTGTCGGAAACCGCACAGCATGCATTACAGCG
AGCTGCCAATGAGTTGCATGCTTTATTTATGCATGCCACGGATTATGTGTTGGAAAACCAAGCGCTATTAAATAAATTTA
ATTTACCTGTCGCGATTTTACCCAAAATACGTCAGTCATGGGATAACCGTTTAAATGAACTAATTACCAGTCGTTTTGAC
TTTGCCATGACCCCACAAGGGTTAAAGGTCTATGAGTACAATTGTGATTCAGCCTCCTGTTATATGGAAAGTGCCAAAAT
TCAGGGTAAATGGGCTCGCCATTATGGTGTTCAGGAAGGACAGGACAGTGGCAAAGATCTTTTTAATCATCTGGTTAACG
CATGGCGTAAAAGTCAGGCAAAAGGACTGGTGCATATTTTGCATGATAATGACCCAGAAGAAAAATACCATGCGTTATTT
ATACAGCAAACATTAAAATCGGCAGGGCTCGAGAGTAAGCTTATCTCTGCTATAAGCAGTCTGCGATGGAATAGTTCGGG
TGATATCTTAGATGAGGAAGGGCAATTAATTCACTGGGTGTGGAAAACCTGGTCCTGGGAAACGGCTCTGGGTCAAATTC
GTGCACAATGTGAAGTGGATCAAGCGCTTGCGGAAAAATATCAACCTCTGTGGCAACAGGTTAGTCCCCCGCGTCTGGCG
GATGTATTACTGCATAAAAATATTATGATTTTTGAGCCGTTATGGACCCTCATCCCCAGTAATAAAGCAATTCTTCCTGT
GCTTTGGTCACTCTTCCCAAATCATCCTCTGTTATTGCATACTAATTTTGTTTTAAATGCTGATTTACAGGCGAGCGGTT
ATGTCAGTAAGCCAATTGTTGGGCGCTGCGGGGCCAACATTCAGGTGGTAAATCATCACGAAGATATTCTTGAGGAGACG
GGAGGGCAATTTTCGGCACAGGATCAAATCTATCAGGCATTATTTCCACTGCCACTGGTGGATGGTTATTATGTACAGGT
CTGCGCCTTTACGGCCGCTGGTAAATATGCGGGCAGTTGTCTGCGTGTTGACTCCTCGATGATCATCAGTAAAGACAGTG
ATTGTATGGCATTACGTTTTGTAAATGATGATCATATTCTAAGTCAATAA

Upstream 100 bases:

>100_bases
ATTGAACTTGAGCTAAGGCTTTAAAATGGTGTCAATTTATATTGCCAATGCAAATTGACTTATTTATATTTTTTGTTAAT
ACCTGATATAGGCTAAAATT

Downstream 100 bases:

>100_bases
TAGGGTATTGATGACATTTTTTACAATACTTCCAGGGTGATTATGGGCTTAGCACTGGGTATTGCCAACAAAAATTCCAT
TAGTTTATAGCGAATAATCT

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MNYKSPSVFGTLLGYAPGGVAAYSSDYDTASDIKYPKRSAFRSYFNGIYMGYKWQCVEFARRWMYINKGTLFDDVAMAYE
IFDLRTVRNLATNSLLPLQAFCNGSKSLPQPGSLLIWDQGGEFAETGHVAIVTEVFPDKLRIVEQNLDHQIWPEGHSFSR
ELPATTTEEGEYWLKCSFNDATILGWMTQTEDDRDAEPTLETNKQLFNLISRRAKEQPNPQKSWLNVANEDEAAYVKAMH
GHKLSAVTADQYRYYVLSETAQHALQRAANELHALFMHATDYVLENQALLNKFNLPVAILPKIRQSWDNRLNELITSRFD
FAMTPQGLKVYEYNCDSASCYMESAKIQGKWARHYGVQEGQDSGKDLFNHLVNAWRKSQAKGLVHILHDNDPEEKYHALF
IQQTLKSAGLESKLISAISSLRWNSSGDILDEEGQLIHWVWKTWSWETALGQIRAQCEVDQALAEKYQPLWQQVSPPRLA
DVLLHKNIMIFEPLWTLIPSNKAILPVLWSLFPNHPLLLHTNFVLNADLQASGYVSKPIVGRCGANIQVVNHHEDILEET
GGQFSAQDQIYQALFPLPLVDGYYVQVCAFTAAGKYAGSCLRVDSSMIISKDSDCMALRFVNDDHILSQ

Sequences:

>Translated_629_residues
MNYKSPSVFGTLLGYAPGGVAAYSSDYDTASDIKYPKRSAFRSYFNGIYMGYKWQCVEFARRWMYINKGTLFDDVAMAYE
IFDLRTVRNLATNSLLPLQAFCNGSKSLPQPGSLLIWDQGGEFAETGHVAIVTEVFPDKLRIVEQNLDHQIWPEGHSFSR
ELPATTTEEGEYWLKCSFNDATILGWMTQTEDDRDAEPTLETNKQLFNLISRRAKEQPNPQKSWLNVANEDEAAYVKAMH
GHKLSAVTADQYRYYVLSETAQHALQRAANELHALFMHATDYVLENQALLNKFNLPVAILPKIRQSWDNRLNELITSRFD
FAMTPQGLKVYEYNCDSASCYMESAKIQGKWARHYGVQEGQDSGKDLFNHLVNAWRKSQAKGLVHILHDNDPEEKYHALF
IQQTLKSAGLESKLISAISSLRWNSSGDILDEEGQLIHWVWKTWSWETALGQIRAQCEVDQALAEKYQPLWQQVSPPRLA
DVLLHKNIMIFEPLWTLIPSNKAILPVLWSLFPNHPLLLHTNFVLNADLQASGYVSKPIVGRCGANIQVVNHHEDILEET
GGQFSAQDQIYQALFPLPLVDGYYVQVCAFTAAGKYAGSCLRVDSSMIISKDSDCMALRFVNDDHILSQ
>Mature_629_residues
MNYKSPSVFGTLLGYAPGGVAAYSSDYDTASDIKYPKRSAFRSYFNGIYMGYKWQCVEFARRWMYINKGTLFDDVAMAYE
IFDLRTVRNLATNSLLPLQAFCNGSKSLPQPGSLLIWDQGGEFAETGHVAIVTEVFPDKLRIVEQNLDHQIWPEGHSFSR
ELPATTTEEGEYWLKCSFNDATILGWMTQTEDDRDAEPTLETNKQLFNLISRRAKEQPNPQKSWLNVANEDEAAYVKAMH
GHKLSAVTADQYRYYVLSETAQHALQRAANELHALFMHATDYVLENQALLNKFNLPVAILPKIRQSWDNRLNELITSRFD
FAMTPQGLKVYEYNCDSASCYMESAKIQGKWARHYGVQEGQDSGKDLFNHLVNAWRKSQAKGLVHILHDNDPEEKYHALF
IQQTLKSAGLESKLISAISSLRWNSSGDILDEEGQLIHWVWKTWSWETALGQIRAQCEVDQALAEKYQPLWQQVSPPRLA
DVLLHKNIMIFEPLWTLIPSNKAILPVLWSLFPNHPLLLHTNFVLNADLQASGYVSKPIVGRCGANIQVVNHHEDILEET
GGQFSAQDQIYQALFPLPLVDGYYVQVCAFTAAGKYAGSCLRVDSSMIISKDSDCMALRFVNDDHILSQ

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=613, Percent_Identity=47.6345840130506, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789416, Length=364, Percent_Identity=26.6483516483516, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790629, Length=382, Percent_Identity=25.6544502617801, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 71402; Mature: 71402

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKSPSVFGTLLGYAPGGVAAYSSDYDTASDIKYPKRSAFRSYFNGIYMGYKWQCVEFA
CCCCCCHHHHHHHHCCCCCCEEECCCCCCHHCCCCCHHHHHHHHHCCEECCCEEHHHHHH
RRWMYINKGTLFDDVAMAYEIFDLRTVRNLATNSLLPLQAFCNGSKSLPQPGSLLIWDQG
HHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEECCC
GEFAETGHVAIVTEVFPDKLRIVEQNLDHQIWPEGHSFSRELPATTTEEGEYWLKCSFND
CCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
ATILGWMTQTEDDRDAEPTLETNKQLFNLISRRAKEQPNPQKSWLNVANEDEAAYVKAMH
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHC
GHKLSAVTADQYRYYVLSETAQHALQRAANELHALFMHATDYVLENQALLNKFNLPVAIL
CCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEH
PKIRQSWDNRLNELITSRFDFAMTPQGLKVYEYNCDSASCYMESAKIQGKWARHYGVQEG
HHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCC
QDSGKDLFNHLVNAWRKSQAKGLVHILHDNDPEEKYHALFIQQTLKSAGLESKLISAISS
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LRWNSSGDILDEEGQLIHWVWKTWSWETALGQIRAQCEVDQALAEKYQPLWQQVSPPRLA
HCCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
DVLLHKNIMIFEPLWTLIPSNKAILPVLWSLFPNHPLLLHTNFVLNADLQASGYVSKPIV
HHHHHCCEEEECHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCH
GRCGANIQVVNHHEDILEETGGQFSAQDQIYQALFPLPLVDGYYVQVCAFTAAGKYAGSC
HHCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHHCCHHCCCE
LRVDSSMIISKDSDCMALRFVNDDHILSQ
EEECCCEEEECCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MNYKSPSVFGTLLGYAPGGVAAYSSDYDTASDIKYPKRSAFRSYFNGIYMGYKWQCVEFA
CCCCCCHHHHHHHHCCCCCCEEECCCCCCHHCCCCCHHHHHHHHHCCEECCCEEHHHHHH
RRWMYINKGTLFDDVAMAYEIFDLRTVRNLATNSLLPLQAFCNGSKSLPQPGSLLIWDQG
HHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEECCC
GEFAETGHVAIVTEVFPDKLRIVEQNLDHQIWPEGHSFSRELPATTTEEGEYWLKCSFND
CCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
ATILGWMTQTEDDRDAEPTLETNKQLFNLISRRAKEQPNPQKSWLNVANEDEAAYVKAMH
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHC
GHKLSAVTADQYRYYVLSETAQHALQRAANELHALFMHATDYVLENQALLNKFNLPVAIL
CCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEH
PKIRQSWDNRLNELITSRFDFAMTPQGLKVYEYNCDSASCYMESAKIQGKWARHYGVQEG
HHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCC
QDSGKDLFNHLVNAWRKSQAKGLVHILHDNDPEEKYHALFIQQTLKSAGLESKLISAISS
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LRWNSSGDILDEEGQLIHWVWKTWSWETALGQIRAQCEVDQALAEKYQPLWQQVSPPRLA
HCCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
DVLLHKNIMIFEPLWTLIPSNKAILPVLWSLFPNHPLLLHTNFVLNADLQASGYVSKPIV
HHHHHCCEEEECHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCH
GRCGANIQVVNHHEDILEETGGQFSAQDQIYQALFPLPLVDGYYVQVCAFTAAGKYAGSC
HHCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHHCCHHCCCE
LRVDSSMIISKDSDCMALRFVNDDHILSQ
EEECCCEEEECCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]