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The map label for this gene is rotA [H]

Identifier: 119944838

GI number: 119944838

Start: 1351088

End: 1351630

Strand: Reverse

Name: rotA [H]

Synonym: Ping_1080

Alternate gene names: 119944838

Gene position: 1351630-1351088 (Counterclockwise)

Preceding gene: 119944839

Following gene: 119944834

Centisome position: 29.64

GC content: 37.94

Gene sequence:

>543_bases
ATGAAACACTTAATATCTATTTTTCTGCTCTTTCTTAGCTTCTCTGTATTTTCAGACGAAAATCCAACTGTTTTAATGGA
AACATCCCAAGGCCCCATTATTATTGAATTGTATCCAACACTAGCCCCCAAAACAGTGGACAATTTTTTAAAATATGTAC
AAACAGATGGTTTTAAGCAAACTACCTTCCACCGTATTATTAATAATTTTATGATCCAGGGAGGAGGTTTTGAGGTGTCT
GGAAAAAAAGCGGTCACTTTTGCAGCAATAGAGAATGAATCCCGCAATGGTTTAAGCAACAAACGCGGCACCATTGCCAT
GGCAAGAACCAACAATCCGCATTCTGCCACTCGCCAATTTTTTATTAATCACAATGATAATCATTTTTTAGATGCACAAG
ATGAACGCTGGGGTTACAGTGTATTCGGCAAAGTGATTAAAGGGATGGAGGTTGTCGATAAAATAGCAATGGTCAAGACA
GGCGCTGGCGATAAACCCATTCAGACTGTTATTATTAATCGCATCTCAGCAGGTAAAAAATAA

Upstream 100 bases:

>100_bases
TAATAAGCAGTTATCACTATTGTTAACTGAAATAGTGATAGATCCAGAACTTAATACCAAGCTGCAAAAATTCTAATTTA
TATCAATACAAGGAAACACC

Downstream 100 bases:

>100_bases
GTATAAAGAATCAGCACTTAAGTAAGTGCTGATTGGAAATTTTTTATACTCAAAGCAGGGAGTTAGCAAACTAACTCCCT
GCTAGTTTAGTATAATGGAC

Product: peptidyl-prolyl cis-trans isomerase, cyclophilin type

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MKHLISIFLLFLSFSVFSDENPTVLMETSQGPIIIELYPTLAPKTVDNFLKYVQTDGFKQTTFHRIINNFMIQGGGFEVS
GKKAVTFAAIENESRNGLSNKRGTIAMARTNNPHSATRQFFINHNDNHFLDAQDERWGYSVFGKVIKGMEVVDKIAMVKT
GAGDKPIQTVIINRISAGKK

Sequences:

>Translated_180_residues
MKHLISIFLLFLSFSVFSDENPTVLMETSQGPIIIELYPTLAPKTVDNFLKYVQTDGFKQTTFHRIINNFMIQGGGFEVS
GKKAVTFAAIENESRNGLSNKRGTIAMARTNNPHSATRQFFINHNDNHFLDAQDERWGYSVFGKVIKGMEVVDKIAMVKT
GAGDKPIQTVIINRISAGKK
>Mature_180_residues
MKHLISIFLLFLSFSVFSDENPTVLMETSQGPIIIELYPTLAPKTVDNFLKYVQTDGFKQTTFHRIINNFMIQGGGFEVS
GKKAVTFAAIENESRNGLSNKRGTIAMARTNNPHSATRQFFINHNDNHFLDAQDERWGYSVFGKVIKGMEVVDKIAMVKT
GAGDKPIQTVIINRISAGKK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7706339, Length=158, Percent_Identity=34.8101265822785, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI4758950, Length=185, Percent_Identity=33.5135135135135, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI5454154, Length=174, Percent_Identity=32.183908045977, Blast_Score=81, Evalue=5e-16,
Organism=Homo sapiens, GI24308049, Length=158, Percent_Identity=35.4430379746835, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI19557636, Length=158, Percent_Identity=32.2784810126582, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4505991, Length=155, Percent_Identity=33.5483870967742, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI22547212, Length=154, Percent_Identity=31.1688311688312, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI7657473, Length=154, Percent_Identity=31.1688311688312, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI22547215, Length=154, Percent_Identity=31.1688311688312, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI6631100, Length=153, Percent_Identity=32.6797385620915, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI42560244, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1786736, Length=160, Percent_Identity=50.625, Blast_Score=150, Evalue=4e-38,
Organism=Escherichia coli, GI1789763, Length=140, Percent_Identity=53.5714285714286, Blast_Score=143, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17552780, Length=148, Percent_Identity=35.8108108108108, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71980590, Length=148, Percent_Identity=35.1351351351351, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71980594, Length=148, Percent_Identity=35.1351351351351, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17532643, Length=169, Percent_Identity=31.9526627218935, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17539496, Length=151, Percent_Identity=31.1258278145695, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17506307, Length=148, Percent_Identity=33.7837837837838, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17532641, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565860, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551282, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551284, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559074, Length=157, Percent_Identity=29.9363057324841, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539498, Length=148, Percent_Identity=32.4324324324324, Blast_Score=66, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17506311, Length=162, Percent_Identity=32.0987654320988, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321848, Length=196, Percent_Identity=31.1224489795918, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320510, Length=190, Percent_Identity=31.0526315789474, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323562, Length=151, Percent_Identity=34.4370860927152, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320359, Length=164, Percent_Identity=27.4390243902439, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17986117, Length=151, Percent_Identity=34.4370860927152, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922376, Length=152, Percent_Identity=30.2631578947368, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI20130249, Length=148, Percent_Identity=35.1351351351351, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24586038, Length=174, Percent_Identity=29.8850574712644, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17647301, Length=163, Percent_Identity=32.5153374233129, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136360, Length=152, Percent_Identity=29.6052631578947, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20129; Mature: 20129

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLISIFLLFLSFSVFSDENPTVLMETSQGPIIIELYPTLAPKTVDNFLKYVQTDGFKQ
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCH
TTFHRIINNFMIQGGGFEVSGKKAVTFAAIENESRNGLSNKRGTIAMARTNNPHSATRQF
HHHHHHHHHHEECCCCEEECCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHEEE
FINHNDNHFLDAQDERWGYSVFGKVIKGMEVVDKIAMVKTGAGDKPIQTVIINRISAGKK
EEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKHLISIFLLFLSFSVFSDENPTVLMETSQGPIIIELYPTLAPKTVDNFLKYVQTDGFKQ
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCH
TTFHRIINNFMIQGGGFEVSGKKAVTFAAIENESRNGLSNKRGTIAMARTNNPHSATRQF
HHHHHHHHHHEECCCCEEECCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHEEE
FINHNDNHFLDAQDERWGYSVFGKVIKGMEVVDKIAMVKTGAGDKPIQTVIINRISAGKK
EEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]