Click here to switch to the map view.

The map label for this gene is rsmA

Identifier: 119944805

GI number: 119944805

Start: 1319803

End: 1320615

Strand: Reverse

Name: rsmA

Synonym: Ping_1047

Alternate gene names: 119944805

Gene position: 1320615-1319803 (Counterclockwise)

Preceding gene: 119944806

Following gene: 119944804

Centisome position: 28.96

GC content: 40.1

Gene sequence:

>813_bases
ATGAATGATAAAACACACTTAGGTCATACCGCGCGTAAACGTTTTGGACAAAACTTCTTACACGATGATTATATTATTGA
TTCTATTGTTGGGGCGATCGCACCTCAATGGGAAGACAACATCGTTGAAATAGGCCCGGGCTTAGGCGCACTAACAGAGC
CGGTTGCAAGTAAAGTGAAGTGCTTAAATGTCATTGAGCTCGATAGAGATTTGGCTGCCCGTTTAGCAGAGCATCCCGTA
TTAGGTGATAAATTAAATATTACCCAAGCCGATGCGCTACAATTTGATTTTGGAAAGTTAGCCAGTACAGAAAGACCACT
GCGCGTTTTTGGTAACTTGCCCTATAATATTTCAACACCGCTGATGTTTCATTTGTTCGAATATGCAGACAAAATTTCTG
ATATGCATTTTATGCTCCAAAAAGAAGTGGTTAATCGTCTCTGTGCAGGTCCGAACTGTAAAGCCTACGGACGCTTAACC
GTTATGGCCCAATATTATTGCCGCATTATTCCTGTATTAGAAGTACCACCGACGGCTTTTAAACCGGCTCCTAAGGTTGA
TTCAGCTGTTGTACGTTTAGAGCCCTATGACACCCCACCCTTTATCGCTAAAAGCTTAAAATGCCTTACCCAAGTGTGTT
CCATGGCCTTTAATCAACGCCGTAAAACAATTCGTAATGGTTTGCGTGATTTGTTAAGTATTGAACAATTACAAGAAATT
GGCATTGATACAACCAAACGGGCAGAAAATATCAGCGTTGAAGAGTACGTTAATATTGCCAATTATGTGTTTGATTTAAA
AGAAAATAAATAA

Upstream 100 bases:

>100_bases
TAGTTAAATAACTGTTTTATTTTGCGGGTTAACTTTCCGAGCTAACCCTGAGCAGAACAGATAATTAATATTTTGGTATA
AGCAACAAGGCAAAATCAAT

Downstream 100 bases:

>100_bases
AGGTGGCTACCTATATAGTGGGTGATGTCCACGGCTGCTTCGATGAACTTCAAGCTTTGCTTGAACTCGCGCAATTTAAA
AAAAATAAAGATCAGCTTTG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNDKTHLGHTARKRFGQNFLHDDYIIDSIVGAIAPQWEDNIVEIGPGLGALTEPVASKVKCLNVIELDRDLAARLAEHPV
LGDKLNITQADALQFDFGKLASTERPLRVFGNLPYNISTPLMFHLFEYADKISDMHFMLQKEVVNRLCAGPNCKAYGRLT
VMAQYYCRIIPVLEVPPTAFKPAPKVDSAVVRLEPYDTPPFIAKSLKCLTQVCSMAFNQRRKTIRNGLRDLLSIEQLQEI
GIDTTKRAENISVEEYVNIANYVFDLKENK

Sequences:

>Translated_270_residues
MNDKTHLGHTARKRFGQNFLHDDYIIDSIVGAIAPQWEDNIVEIGPGLGALTEPVASKVKCLNVIELDRDLAARLAEHPV
LGDKLNITQADALQFDFGKLASTERPLRVFGNLPYNISTPLMFHLFEYADKISDMHFMLQKEVVNRLCAGPNCKAYGRLT
VMAQYYCRIIPVLEVPPTAFKPAPKVDSAVVRLEPYDTPPFIAKSLKCLTQVCSMAFNQRRKTIRNGLRDLLSIEQLQEI
GIDTTKRAENISVEEYVNIANYVFDLKENK
>Mature_270_residues
MNDKTHLGHTARKRFGQNFLHDDYIIDSIVGAIAPQWEDNIVEIGPGLGALTEPVASKVKCLNVIELDRDLAARLAEHPV
LGDKLNITQADALQFDFGKLASTERPLRVFGNLPYNISTPLMFHLFEYADKISDMHFMLQKEVVNRLCAGPNCKAYGRLT
VMAQYYCRIIPVLEVPPTAFKPAPKVDSAVVRLEPYDTPPFIAKSLKCLTQVCSMAFNQRRKTIRNGLRDLLSIEQLQEI
GIDTTKRAENISVEEYVNIANYVFDLKENK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=281, Percent_Identity=31.6725978647687, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI7657198, Length=250, Percent_Identity=31.6, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=67.0454545454545, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141369, Length=284, Percent_Identity=28.8732394366197, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=209, Percent_Identity=33.0143540669856, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=227, Percent_Identity=27.7533039647577, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358017, Length=271, Percent_Identity=29.1512915129151, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357273, Length=280, Percent_Identity=29.2857142857143, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PSYIN (A1STS1)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942485.1
- ProteinModelPortal:   A1STS1
- SMR:   A1STS1
- STRING:   A1STS1
- GeneID:   4623907
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1047
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   A1STS1
- BioCyc:   PING357804:PING_1047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30452; Mature: 30452

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 91-91 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKTHLGHTARKRFGQNFLHDDYIIDSIVGAIAPQWEDNIVEIGPGLGALTEPVASKVK
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHH
CLNVIELDRDLAARLAEHPVLGDKLNITQADALQFDFGKLASTERPLRVFGNLPYNISTP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHH
LMFHLFEYADKISDMHFMLQKEVVNRLCAGPNCKAYGRLTVMAQYYCRIIPVLEVPPTAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KPAPKVDSAVVRLEPYDTPPFIAKSLKCLTQVCSMAFNQRRKTIRNGLRDLLSIEQLQEI
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDTTKRAENISVEEYVNIANYVFDLKENK
CCCCHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDKTHLGHTARKRFGQNFLHDDYIIDSIVGAIAPQWEDNIVEIGPGLGALTEPVASKVK
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHH
CLNVIELDRDLAARLAEHPVLGDKLNITQADALQFDFGKLASTERPLRVFGNLPYNISTP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHH
LMFHLFEYADKISDMHFMLQKEVVNRLCAGPNCKAYGRLTVMAQYYCRIIPVLEVPPTAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KPAPKVDSAVVRLEPYDTPPFIAKSLKCLTQVCSMAFNQRRKTIRNGLRDLLSIEQLQEI
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDTTKRAENISVEEYVNIANYVFDLKENK
CCCCHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA