Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 119944741

GI number: 119944741

Start: 1238080

End: 1238742

Strand: Reverse

Name: rluE [H]

Synonym: Ping_0982

Alternate gene names: 119944741

Gene position: 1238742-1238080 (Counterclockwise)

Preceding gene: 119944743

Following gene: 119944740

Centisome position: 27.17

GC content: 40.42

Gene sequence:

>663_bases
GTGGTTAAAACTAAAAATTCCAATAAAAAATCCATCTGGTTTTCGAAAGCTAAAATAGGCAATGCAGCAAAGCACCCTTC
GGTCAAAAACAAAATAAATACACAACAGCTGCCGCCCGTAAGCGATGCAACACGCAAAATACTTTTATTTAATAAACCCT
TTAATGTATTAAGTCAGTTTACTGATCAGGACAATCGTCAAACATTAAAAGACTATATTGATATTCCTGAGGTCTATGCT
GCCGGTCGATTAGATCGTGATAGTGAGGGATTATTATTATTGACCAATGATGGTAAATTACAAGCCAAGATCACCGAACC
CAACGCTAAAACCAGTAAAACTTATTGGGTACAAGTAGAAGGCAGACCGACTGAAGCGGATCTGCAAAAATTACGTGATG
GCGTTAATCTTAAAGATGGTTTAACCCTGCCGGCCGGTATTGAGTTAATGGAACAGCCCGCTATTTGGGCGCGTGTACCC
CCTATTCGCGAACGAGCCAATATTCCGACTACCTGGTTAAGCATTACTATTAGTGAAGGCCGTAATCGTCAGGTACGGCG
TATGACGGCGCATATTGGTTTTCCAACCTTACGGTTGATTCGTTATCGGATTGGTGATTGGACAATTGATGATATTGATA
ATGGGCAATATAAATTGCTATAG

Upstream 100 bases:

>100_bases
ACCCTTTTATATAATAGACAAAGCAGTATGGCTTCCCTTGTTTTGTCTATTAAACCTCTCTTCTGTTACAATTCATTTTT
ATTTATTTTAAGGTGTTTTC

Downstream 100 bases:

>100_bases
GTTTTCTGTTTAGCTTTTACTTGTTGTTTTGATCGACAAATAAACTACACCTCATCGTCATTCTCTCAATGAGTTTGTAA
CCTAGCTGATTTGGTGTGAT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MVKTKNSNKKSIWFSKAKIGNAAKHPSVKNKINTQQLPPVSDATRKILLFNKPFNVLSQFTDQDNRQTLKDYIDIPEVYA
AGRLDRDSEGLLLLTNDGKLQAKITEPNAKTSKTYWVQVEGRPTEADLQKLRDGVNLKDGLTLPAGIELMEQPAIWARVP
PIRERANIPTTWLSITISEGRNRQVRRMTAHIGFPTLRLIRYRIGDWTIDDIDNGQYKLL

Sequences:

>Translated_220_residues
MVKTKNSNKKSIWFSKAKIGNAAKHPSVKNKINTQQLPPVSDATRKILLFNKPFNVLSQFTDQDNRQTLKDYIDIPEVYA
AGRLDRDSEGLLLLTNDGKLQAKITEPNAKTSKTYWVQVEGRPTEADLQKLRDGVNLKDGLTLPAGIELMEQPAIWARVP
PIRERANIPTTWLSITISEGRNRQVRRMTAHIGFPTLRLIRYRIGDWTIDDIDNGQYKLL
>Mature_220_residues
MVKTKNSNKKSIWFSKAKIGNAAKHPSVKNKINTQQLPPVSDATRKILLFNKPFNVLSQFTDQDNRQTLKDYIDIPEVYA
AGRLDRDSEGLLLLTNDGKLQAKITEPNAKTSKTYWVQVEGRPTEADLQKLRDGVNLKDGLTLPAGIELMEQPAIWARVP
PIRERANIPTTWLSITISEGRNRQVRRMTAHIGFPTLRLIRYRIGDWTIDDIDNGQYKLL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=174, Percent_Identity=63.2183908045977, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI1788510, Length=181, Percent_Identity=34.2541436464088, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787524, Length=198, Percent_Identity=31.3131313131313, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1790453, Length=206, Percent_Identity=29.6116504854369, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25048; Mature: 25048

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKTKNSNKKSIWFSKAKIGNAAKHPSVKNKINTQQLPPVSDATRKILLFNKPFNVLSQF
CCCCCCCCCCEEEEEHHHCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
TDQDNRQTLKDYIDIPEVYAAGRLDRDSEGLLLLTNDGKLQAKITEPNAKTSKTYWVQVE
CCCCCHHHHHHHHCCCHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCEEEEEEEC
GRPTEADLQKLRDGVNLKDGLTLPAGIELMEQPAIWARVPPIRERANIPTTWLSITISEG
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEECCCHHHHCCCCEEEEEEEECCC
RNRQVRRMTAHIGFPTLRLIRYRIGDWTIDDIDNGQYKLL
CCCHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCEEEEC
>Mature Secondary Structure
MVKTKNSNKKSIWFSKAKIGNAAKHPSVKNKINTQQLPPVSDATRKILLFNKPFNVLSQF
CCCCCCCCCCEEEEEHHHCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
TDQDNRQTLKDYIDIPEVYAAGRLDRDSEGLLLLTNDGKLQAKITEPNAKTSKTYWVQVE
CCCCCHHHHHHHHCCCHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCEEEEEEEC
GRPTEADLQKLRDGVNLKDGLTLPAGIELMEQPAIWARVPPIRERANIPTTWLSITISEG
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEECCCHHHHCCCCEEEEEEEECCC
RNRQVRRMTAHIGFPTLRLIRYRIGDWTIDDIDNGQYKLL
CCCHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA