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The map label for this gene is pleD [H]

Identifier: 119944737

GI number: 119944737

Start: 1228926

End: 1229894

Strand: Reverse

Name: pleD [H]

Synonym: Ping_0978

Alternate gene names: 119944737

Gene position: 1229894-1228926 (Counterclockwise)

Preceding gene: 119944738

Following gene: 119944736

Centisome position: 26.97

GC content: 38.7

Gene sequence:

>969_bases
ATGCCTGAGAAAGTAAAACAAAAAGTATTAATTGTCGATGATATCAAAGATAATATTAAGGTATTAATCGATCTGCTGAA
ACCAGAATATAAAACATTTTTTGCGACTAATGGTGAAAAAGCCCTTGAATTAGCTCAAAATATGTCCCCTGATATTATCT
TACTGGATATTGTAATGCCAGAAATGGATGGCTTTGAAGTATGCCAGAAATTGAAATCTAACCCCATCACCTGCGATATT
CCTGTTATCTTCATATCGGCAATGAGCGATATTGGGGATGAAACAAAGGGCTTAGAAGTCGGTGCGGTGGATTACATTAC
CAAGCCAATTAGTCCGGCGATCGTTAAAGCCAGGGTGAAAAATCATTTAAAACTTCAGGAAGCCATGCAGGAACTGAAAC
GACTATACAACACAGCACTTGATTCCAATCCCAATACCGGTCTTCCTGGTAATAACAGTGTTGCAAAACAGATTAAAAGT
GCATTGGATACCCAAGAAAATTTGTGCGTTATTTATTCTGATTTGGACAATTTTAAAGCATTCAATGATAAATATGGCTT
TGCTTTGGGGGATGAAGTTATCAAGTTTACCTGCCAGGTATTTCAAGATGTTATCGGTGAGCTAAATATCAAGGATGCAT
TTATCGGTCATATTGGGGGGGACGACTTTGTTCTGATTGTGCCGTCTGACCTGACTCAAACAGTTGCTGAAAAAATCATT
CAACGGTTCGATCATGGTGTTATTAAGTTTTATTCCAGCAAAGATGCAGAAATCAAGTGTATCCAGTCTGTTGACCGCCA
AGGTGAAAGCCAGATCTTCCCGATAATGTCAATCAGTTTGGCAGGTGTTGATTTGGCACATGGCATCTACAAGGAGTACA
TGGAAGTGAATGATGTCTGTGCGACCACCAAAAAAATAGCGAAATCAATGCCAGGGAGTAGCTTTTTTTTAAACAGACGA
GATAAATGA

Upstream 100 bases:

>100_bases
AGAAATTGAGGATCTGGTAAATGATTTACAATTTGAAGAAGCAGCCCTAAAATTGAAAGAGCTGTGCAGTGCACTTGCTC
TTTCGACTTAAGGTGTAATT

Downstream 100 bases:

>100_bases
GTCTGTTGATTTACTGGAATTTTGGTTAGAGAACAACAAAAGATGGGACCCCCAAACCTTTTTTTGAATACCGTACTGGT
TATCTGTAAGCTTTCGCTTG

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPEKVKQKVLIVDDIKDNIKVLIDLLKPEYKTFFATNGEKALELAQNMSPDIILLDIVMPEMDGFEVCQKLKSNPITCDI
PVIFISAMSDIGDETKGLEVGAVDYITKPISPAIVKARVKNHLKLQEAMQELKRLYNTALDSNPNTGLPGNNSVAKQIKS
ALDTQENLCVIYSDLDNFKAFNDKYGFALGDEVIKFTCQVFQDVIGELNIKDAFIGHIGGDDFVLIVPSDLTQTVAEKII
QRFDHGVIKFYSSKDAEIKCIQSVDRQGESQIFPIMSISLAGVDLAHGIYKEYMEVNDVCATTKKIAKSMPGSSFFLNRR
DK

Sequences:

>Translated_322_residues
MPEKVKQKVLIVDDIKDNIKVLIDLLKPEYKTFFATNGEKALELAQNMSPDIILLDIVMPEMDGFEVCQKLKSNPITCDI
PVIFISAMSDIGDETKGLEVGAVDYITKPISPAIVKARVKNHLKLQEAMQELKRLYNTALDSNPNTGLPGNNSVAKQIKS
ALDTQENLCVIYSDLDNFKAFNDKYGFALGDEVIKFTCQVFQDVIGELNIKDAFIGHIGGDDFVLIVPSDLTQTVAEKII
QRFDHGVIKFYSSKDAEIKCIQSVDRQGESQIFPIMSISLAGVDLAHGIYKEYMEVNDVCATTKKIAKSMPGSSFFLNRR
DK
>Mature_321_residues
PEKVKQKVLIVDDIKDNIKVLIDLLKPEYKTFFATNGEKALELAQNMSPDIILLDIVMPEMDGFEVCQKLKSNPITCDIP
VIFISAMSDIGDETKGLEVGAVDYITKPISPAIVKARVKNHLKLQEAMQELKRLYNTALDSNPNTGLPGNNSVAKQIKSA
LDTQENLCVIYSDLDNFKAFNDKYGFALGDEVIKFTCQVFQDVIGELNIKDAFIGHIGGDDFVLIVPSDLTQTVAEKIIQ
RFDHGVIKFYSSKDAEIKCIQSVDRQGESQIFPIMSISLAGVDLAHGIYKEYMEVNDVCATTKKIAKSMPGSSFFLNRRD
K

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=35.9375, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788550, Length=150, Percent_Identity=32.6666666666667, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790860, Length=120, Percent_Identity=34.1666666666667, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786599, Length=107, Percent_Identity=35.5140186915888, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789149, Length=169, Percent_Identity=30.1775147928994, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=123, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082012, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=31.404958677686, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=34.6153846153846, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35839; Mature: 35708

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKVKQKVLIVDDIKDNIKVLIDLLKPEYKTFFATNGEKALELAQNMSPDIILLDIVMP
CCCHHHCCEEEEECCHHHHHHHHHHHCCHHHHEEECCCHHHHHHHHCCCCCEEEEEEECC
EMDGFEVCQKLKSNPITCDIPVIFISAMSDIGDETKGLEVGAVDYITKPISPAIVKARVK
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCEECHHHHHHCCCCHHHHHHHHH
NHLKLQEAMQELKRLYNTALDSNPNTGLPGNNSVAKQIKSALDTQENLCVIYSDLDNFKA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHH
FNDKYGFALGDEVIKFTCQVFQDVIGELNIKDAFIGHIGGDDFVLIVPSDLTQTVAEKII
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCEEEEECCHHHHHHHHHHH
QRFDHGVIKFYSSKDAEIKCIQSVDRQGESQIFPIMSISLAGVDLAHGIYKEYMEVNDVC
HHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATTKKIAKSMPGSSFFLNRRDK
HHHHHHHHHCCCCCEECCCCCC
>Mature Secondary Structure 
PEKVKQKVLIVDDIKDNIKVLIDLLKPEYKTFFATNGEKALELAQNMSPDIILLDIVMP
CCHHHCCEEEEECCHHHHHHHHHHHCCHHHHEEECCCHHHHHHHHCCCCCEEEEEEECC
EMDGFEVCQKLKSNPITCDIPVIFISAMSDIGDETKGLEVGAVDYITKPISPAIVKARVK
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCEECHHHHHHCCCCHHHHHHHHH
NHLKLQEAMQELKRLYNTALDSNPNTGLPGNNSVAKQIKSALDTQENLCVIYSDLDNFKA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHH
FNDKYGFALGDEVIKFTCQVFQDVIGELNIKDAFIGHIGGDDFVLIVPSDLTQTVAEKII
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCEEEEECCHHHHHHHHHHH
QRFDHGVIKFYSSKDAEIKCIQSVDRQGESQIFPIMSISLAGVDLAHGIYKEYMEVNDVC
HHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATTKKIAKSMPGSSFFLNRRDK
HHHHHHHHHCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA