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The map label for this gene is opuD [H]

Identifier: 119944728

GI number: 119944728

Start: 1216460

End: 1218037

Strand: Reverse

Name: opuD [H]

Synonym: Ping_0969

Alternate gene names: 119944728

Gene position: 1218037-1216460 (Counterclockwise)

Preceding gene: 119944729

Following gene: 119944724

Centisome position: 26.71

GC content: 43.09

Gene sequence:

>1578_bases
ATGAAGACAAATAAGGATAAATACAGTATAGATAATACCGATTATACTGTCGGACAGGATAACGTTCAAAAATGGGGTTT
TGATATACATAACCCTGTATTTGGTGCTAGTGCAGGTTTAATCTCACTCTTTTTAATTACAATTTTAGTTATCGATCCGG
CAATATCAAAAGATTTTTTAGATGGTTTAAAATGGAACATTATAGGAACATTTGATAGCTTCTTTATGTGGTCGGCCAAT
TTTTTTGTAATCTTCTGTTTGGCATTAATTGTCTCTCCTTTCGGTAAAATTCGCTTAGGTGGCGACGATGCAAAAGCGGA
TCATTCAAGACTTTCCTGGATAGCGATGCTTTTTGCTGCAGGGATGGGGATTGGTTTGATGTTTTGGGGTGTTGCTGAGC
CCGCAGCGTATTACACAGGCTGGTATGAAACGCCACTAAATGTAGTCGCTAATACACCGGCTGCAGCAGAGCTTGCATTA
GGTGCAACTATGTACCACTGGGGATTACACCCTTGGGCGATTTATGGCGTAGTGGCCTTATCCTTATCATTCTTTGCTTA
TAATAAAGGCTTGCCGCTTTCAATACGATCTGCTTTTTATCCGATATTGGGAGACCGTACTTGGGGCTGGCCGGGACATA
TCATTGATGTGTTAGCCGTATTAGCCACATTATTTGGTCTAGCAACATCACTAGGCTTAGGCGCGCAGCAAGCTGCTGGC
GGAATCCAGCATGTATTTGGCATTGAAGCCGACCTCACCCTGCAAATCACTATTATTGTGGTGGTGACTATGCTGGCTAT
TGTCTCGGTGGTACGTGGCCTTGAAGGTGGTGTTAAATTAATCAGTAATGTCAATATGTTAATTGCTGTTGTTTTAGTCG
TTTTTGTTGCACTCGTGACCCTGGCGGTTTCGATCGGCTCTATCCCCACAACTATAATGGGTTATGTTAAGAATATTATC
CCCTTGAGTAATCCAATGGGTCGCGATGACGAAGCCTGGATGCAAGGTTGGACGGTCTTTTATTGGGCATGGTGGATTTC
ATGGTCTCCTTTTGTGGGTATGTTTATTGCGCGGATATCGCGCGGCCGTACGGTACGTGAATTTCTTACCGCAGTAATGA
TTATACCAACAGCAGTCACTATTTTATGGATGTCTGTATTTGGTGGTGTCGCTATTGACCAAATTGTCAATAATGTCGGC
ATATTGGGGAGTGAAGGTTTAACGGACGTTTCGCTTGCCATGTTCCAGATGTTTGATGCTTTGCCATTAGGTAGCATCTT
ATCAATGATAGCTGTGCTACTGGTAATGGTTTTCTTTATTACCTCTTCCGATTCCGGTTCGCTGGTTATAGACAGTATTA
CTGCGGGTGGTAAAATTGATGCGCCGATTCCACAACGTATTTTTTGGGCAACAATCGAGGGTGCAGTTGCGGCTGTTTTA
CTCTGGGTTGGTGGCACTGAGGCGATTGAGGCATTACAAGCGGGTGCTATTTCGACTGGCCTACCCTTTACCATTATTCT
ATTAGTAATGTGTGTCAGTTTGTTGATGGGTATGCGCACAGAAAAAAATAAAAGCTAG

Upstream 100 bases:

>100_bases
GAAATAAGCCTGATATTATCATCTGATTTTGTTAAAATATTTTTGGAAAATTCAATCGACTGTTACATTAACAGAGCCAT
GTCGCTCTAAGCGAGGATGT

Downstream 100 bases:

>100_bases
TTTTTACTCGCGATAAAAAAAGCCGATGAATTATTCATCGGCTTTTTTTTTACCTATTAATCTTCGCTAATCAGCTTAGA
CTTTATAACCATAATTTTTT

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKTNKDKYSIDNTDYTVGQDNVQKWGFDIHNPVFGASAGLISLFLITILVIDPAISKDFLDGLKWNIIGTFDSFFMWSAN
FFVIFCLALIVSPFGKIRLGGDDAKADHSRLSWIAMLFAAGMGIGLMFWGVAEPAAYYTGWYETPLNVVANTPAAAELAL
GATMYHWGLHPWAIYGVVALSLSFFAYNKGLPLSIRSAFYPILGDRTWGWPGHIIDVLAVLATLFGLATSLGLGAQQAAG
GIQHVFGIEADLTLQITIIVVVTMLAIVSVVRGLEGGVKLISNVNMLIAVVLVVFVALVTLAVSIGSIPTTIMGYVKNII
PLSNPMGRDDEAWMQGWTVFYWAWWISWSPFVGMFIARISRGRTVREFLTAVMIIPTAVTILWMSVFGGVAIDQIVNNVG
ILGSEGLTDVSLAMFQMFDALPLGSILSMIAVLLVMVFFITSSDSGSLVIDSITAGGKIDAPIPQRIFWATIEGAVAAVL
LWVGGTEAIEALQAGAISTGLPFTIILLVMCVSLLMGMRTEKNKS

Sequences:

>Translated_525_residues
MKTNKDKYSIDNTDYTVGQDNVQKWGFDIHNPVFGASAGLISLFLITILVIDPAISKDFLDGLKWNIIGTFDSFFMWSAN
FFVIFCLALIVSPFGKIRLGGDDAKADHSRLSWIAMLFAAGMGIGLMFWGVAEPAAYYTGWYETPLNVVANTPAAAELAL
GATMYHWGLHPWAIYGVVALSLSFFAYNKGLPLSIRSAFYPILGDRTWGWPGHIIDVLAVLATLFGLATSLGLGAQQAAG
GIQHVFGIEADLTLQITIIVVVTMLAIVSVVRGLEGGVKLISNVNMLIAVVLVVFVALVTLAVSIGSIPTTIMGYVKNII
PLSNPMGRDDEAWMQGWTVFYWAWWISWSPFVGMFIARISRGRTVREFLTAVMIIPTAVTILWMSVFGGVAIDQIVNNVG
ILGSEGLTDVSLAMFQMFDALPLGSILSMIAVLLVMVFFITSSDSGSLVIDSITAGGKIDAPIPQRIFWATIEGAVAAVL
LWVGGTEAIEALQAGAISTGLPFTIILLVMCVSLLMGMRTEKNKS
>Mature_525_residues
MKTNKDKYSIDNTDYTVGQDNVQKWGFDIHNPVFGASAGLISLFLITILVIDPAISKDFLDGLKWNIIGTFDSFFMWSAN
FFVIFCLALIVSPFGKIRLGGDDAKADHSRLSWIAMLFAAGMGIGLMFWGVAEPAAYYTGWYETPLNVVANTPAAAELAL
GATMYHWGLHPWAIYGVVALSLSFFAYNKGLPLSIRSAFYPILGDRTWGWPGHIIDVLAVLATLFGLATSLGLGAQQAAG
GIQHVFGIEADLTLQITIIVVVTMLAIVSVVRGLEGGVKLISNVNMLIAVVLVVFVALVTLAVSIGSIPTTIMGYVKNII
PLSNPMGRDDEAWMQGWTVFYWAWWISWSPFVGMFIARISRGRTVREFLTAVMIIPTAVTILWMSVFGGVAIDQIVNNVG
ILGSEGLTDVSLAMFQMFDALPLGSILSMIAVLLVMVFFITSSDSGSLVIDSITAGGKIDAPIPQRIFWATIEGAVAAVL
LWVGGTEAIEALQAGAISTGLPFTIILLVMCVSLLMGMRTEKNKS

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=515, Percent_Identity=35.1456310679612, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1786224, Length=441, Percent_Identity=30.1587301587302, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1788102, Length=438, Percent_Identity=30.1369863013699, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56660; Mature: 56660

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNKDKYSIDNTDYTVGQDNVQKWGFDIHNPVFGASAGLISLFLITILVIDPAISKDFL
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
DGLKWNIIGTFDSFFMWSANFFVIFCLALIVSPFGKIRLGGDDAKADHSRLSWIAMLFAA
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHH
GMGIGLMFWGVAEPAAYYTGWYETPLNVVANTPAAAELALGATMYHWGLHPWAIYGVVAL
HCCHHHHHHHHCCCHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SLSFFAYNKGLPLSIRSAFYPILGDRTWGWPGHIIDVLAVLATLFGLATSLGLGAQQAAG
HHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
GIQHVFGIEADLTLQITIIVVVTMLAIVSVVRGLEGGVKLISNVNMLIAVVLVVFVALVT
CHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAVSIGSIPTTIMGYVKNIIPLSNPMGRDDEAWMQGWTVFYWAWWISWSPFVGMFIARIS
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHH
RGRTVREFLTAVMIIPTAVTILWMSVFGGVAIDQIVNNVGILGSEGLTDVSLAMFQMFDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
LPLGSILSMIAVLLVMVFFITSSDSGSLVIDSITAGGKIDAPIPQRIFWATIEGAVAAVL
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
LWVGGTEAIEALQAGAISTGLPFTIILLVMCVSLLMGMRTEKNKS
HHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKTNKDKYSIDNTDYTVGQDNVQKWGFDIHNPVFGASAGLISLFLITILVIDPAISKDFL
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
DGLKWNIIGTFDSFFMWSANFFVIFCLALIVSPFGKIRLGGDDAKADHSRLSWIAMLFAA
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHH
GMGIGLMFWGVAEPAAYYTGWYETPLNVVANTPAAAELALGATMYHWGLHPWAIYGVVAL
HCCHHHHHHHHCCCHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SLSFFAYNKGLPLSIRSAFYPILGDRTWGWPGHIIDVLAVLATLFGLATSLGLGAQQAAG
HHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
GIQHVFGIEADLTLQITIIVVVTMLAIVSVVRGLEGGVKLISNVNMLIAVVLVVFVALVT
CHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAVSIGSIPTTIMGYVKNIIPLSNPMGRDDEAWMQGWTVFYWAWWISWSPFVGMFIARIS
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHH
RGRTVREFLTAVMIIPTAVTILWMSVFGGVAIDQIVNNVGILGSEGLTDVSLAMFQMFDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
LPLGSILSMIAVLLVMVFFITSSDSGSLVIDSITAGGKIDAPIPQRIFWATIEGAVAAVL
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
LWVGGTEAIEALQAGAISTGLPFTIILLVMCVSLLMGMRTEKNKS
HHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]