| Definition | Psychromonas ingrahamii 37, complete genome. |
|---|---|
| Accession | NC_008709 |
| Length | 4,559,598 |
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The map label for this gene is cysD
Identifier: 119944559
GI number: 119944559
Start: 997166
End: 998074
Strand: Direct
Name: cysD
Synonym: Ping_0796
Alternate gene names: 119944559
Gene position: 997166-998074 (Clockwise)
Preceding gene: 119944558
Following gene: 119944560
Centisome position: 21.87
GC content: 43.34
Gene sequence:
>909_bases ATGAAAAAAAATAGATTAACTCACCTCAAGGCGCTTGAGGCTGAATCAATTCAAATTATGCGTGAGGTCGCGGCTGAATT TGATAACCCGGTGATGCTGTATTCTGTCGGTAAAGATTCTTCAGTATTACTGCATTTGGCACGCAAAGCATTTTACCCAG GGAAAATTCCGTTTCCGTTATTGCATGTTGATACCAACTGGAAATTCAAAGAGATGATTGCATTTCGCGATAACATAGCA AAAAAATATGATTTTGATTTGCTGGTTCATAAAAACCCGCGCGGCATGGAGATGGGGATCAGTCCTTTTGAACACGGTAG TGCTAAACATACGGACATTATGAAAACCGAAGGCTTAAAACAAGCTTTGGATAAATACGGTTTTGATGCGGCCTTCGGCG GCGCACGTCGTGATGAAGAGAAATCTCGTGCAAAAGAGCGTGTTTATTCTTTCCGTGATAAAAAACATCGCTGGGATCCA AAAAACCAGCGTCCTGAATTATGGAATATTTATAACGCTAAAGTGGATCGCGGTGAAAGTATTCGTGTGTTCCCGCTGTC GAACTGGACAGAGCTTGATATCTGGCAGTATATCTATCAGGAAGATATCGAAATGGTGCCTTTATACTTTGCCAAAAAAC GCCCGGTCGTTGAACGTGATGGTGCACTTATTATGGTCGATGATGAACGTATGCCGTTAAAAGAAGGTGAAGTGCCGGAA ATGAAAATGGTCCGTTTCCGTACTCTAGGTTGTTACCCGTTAACCGGTGCGATTGAATCGCAAGCGACAACCCTGCCAGA AATTATCCAGGAAATGCTGTTAACCACCACCTCCGAGCGCCAAGGCCGAGTGATAGACAATGACTCTGCGGGATCTATGG AAAAGAAAAAGATGGAGGGATATTTCTAG
Upstream 100 bases:
>100_bases TCGCTTATTACTTATAACTTTTTATCCCTTAATTATCAGAATATATTGATGCATAATGTGTGATGCATAAAATTAAATAG TGTTAACGCGAAGGAATATC
Downstream 100 bases:
>100_bases CGCTCCGCGCTAGAAATAGCTGTCGGCTTTCAGCGGTCGGCTGTCGGTTTTTAAACAACCTTATCAATTAATATGTGCTA GACCATATAGAGAGATCAAA
Product: sulfate adenylyltransferase subunit 2
Products: NA
Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT
Number of amino acids: Translated: 302; Mature: 302
Protein sequence:
>302_residues MKKNRLTHLKALEAESIQIMREVAAEFDNPVMLYSVGKDSSVLLHLARKAFYPGKIPFPLLHVDTNWKFKEMIAFRDNIA KKYDFDLLVHKNPRGMEMGISPFEHGSAKHTDIMKTEGLKQALDKYGFDAAFGGARRDEEKSRAKERVYSFRDKKHRWDP KNQRPELWNIYNAKVDRGESIRVFPLSNWTELDIWQYIYQEDIEMVPLYFAKKRPVVERDGALIMVDDERMPLKEGEVPE MKMVRFRTLGCYPLTGAIESQATTLPEIIQEMLLTTTSERQGRVIDNDSAGSMEKKKMEGYF
Sequences:
>Translated_302_residues MKKNRLTHLKALEAESIQIMREVAAEFDNPVMLYSVGKDSSVLLHLARKAFYPGKIPFPLLHVDTNWKFKEMIAFRDNIA KKYDFDLLVHKNPRGMEMGISPFEHGSAKHTDIMKTEGLKQALDKYGFDAAFGGARRDEEKSRAKERVYSFRDKKHRWDP KNQRPELWNIYNAKVDRGESIRVFPLSNWTELDIWQYIYQEDIEMVPLYFAKKRPVVERDGALIMVDDERMPLKEGEVPE MKMVRFRTLGCYPLTGAIESQATTLPEIIQEMLLTTTSERQGRVIDNDSAGSMEKKKMEGYF >Mature_302_residues MKKNRLTHLKALEAESIQIMREVAAEFDNPVMLYSVGKDSSVLLHLARKAFYPGKIPFPLLHVDTNWKFKEMIAFRDNIA KKYDFDLLVHKNPRGMEMGISPFEHGSAKHTDIMKTEGLKQALDKYGFDAAFGGARRDEEKSRAKERVYSFRDKKHRWDP KNQRPELWNIYNAKVDRGESIRVFPLSNWTELDIWQYIYQEDIEMVPLYFAKKRPVVERDGALIMVDDERMPLKEGEVPE MKMVRFRTLGCYPLTGAIESQATTLPEIIQEMLLTTTSERQGRVIDNDSAGSMEKKKMEGYF
Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]
COG id: COG0175
COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PAPS reductase family. CysD subfamily
Homologues:
Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=76.158940397351, Blast_Score=494, Evalue=1e-141,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): CYSD_PSYIN (A1ST25)
Other databases:
- EMBL: CP000510 - RefSeq: YP_942239.1 - ProteinModelPortal: A1ST25 - SMR: A1ST25 - STRING: A1ST25 - GeneID: 4626369 - GenomeReviews: CP000510_GR - KEGG: pin:Ping_0796 - eggNOG: COG0175 - HOGENOM: HBG758022 - OMA: GMIRFRT - PhylomeDB: A1ST25 - ProtClustDB: PRK05253 - BioCyc: PING357804:PING_0796-MONOMER - HAMAP: MF_00064 - InterPro: IPR002500 - InterPro: IPR014729 - InterPro: IPR011784 - Gene3D: G3DSA:3.40.50.620 - PIRSF: PIRSF002936 - TIGRFAMs: TIGR02039
Pfam domain/function: PF01507 PAPS_reduct
EC number: =2.7.7.4
Molecular weight: Translated: 35165; Mature: 35165
Theoretical pI: Translated: 8.78; Mature: 8.78
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 5.0 %Met (Translated Protein) 5.3 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 5.0 %Met (Mature Protein) 5.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKNRLTHLKALEAESIQIMREVAAEFDNPVMLYSVGKDSSVLLHLARKAFYPGKIPFPL CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE LHVDTNWKFKEMIAFRDNIAKKYDFDLLVHKNPRGMEMGISPFEHGSAKHTDIMKTEGLK EEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH QALDKYGFDAAFGGARRDEEKSRAKERVYSFRDKKHRWDPKNQRPELWNIYNAKVDRGES HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCE IRVFPLSNWTELDIWQYIYQEDIEMVPLYFAKKRPVVERDGALIMVDDERMPLKEGEVPE EEEEECCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCEECCCCEEEECCCCCCCCCCCCCC MKMVRFRTLGCYPLTGAIESQATTLPEIIQEMLLTTTSERQGRVIDNDSAGSMEKKKMEG HHHHHHHCCCCEECCCCCCHHHCHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHCC YF CC >Mature Secondary Structure MKKNRLTHLKALEAESIQIMREVAAEFDNPVMLYSVGKDSSVLLHLARKAFYPGKIPFPL CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE LHVDTNWKFKEMIAFRDNIAKKYDFDLLVHKNPRGMEMGISPFEHGSAKHTDIMKTEGLK EEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH QALDKYGFDAAFGGARRDEEKSRAKERVYSFRDKKHRWDPKNQRPELWNIYNAKVDRGES HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCE IRVFPLSNWTELDIWQYIYQEDIEMVPLYFAKKRPVVERDGALIMVDDERMPLKEGEVPE EEEEECCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCEECCCCEEEECCCCCCCCCCCCCC MKMVRFRTLGCYPLTGAIESQATTLPEIIQEMLLTTTSERQGRVIDNDSAGSMEKKKMEG HHHHHHHCCCCEECCCCCCHHHCHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHCC YF CC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA