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The map label for this gene is ubiF [H]

Identifier: 119944309

GI number: 119944309

Start: 684895

End: 686052

Strand: Direct

Name: ubiF [H]

Synonym: Ping_0536

Alternate gene names: 119944309

Gene position: 684895-686052 (Clockwise)

Preceding gene: 119944303

Following gene: 119944315

Centisome position: 15.02

GC content: 43.96

Gene sequence:

>1158_bases
ATGCAACGCAGAGAAATAATTGTCGTCGGTGGAGGGATGGTCGGGGCCCTAAGTGCACTATTGTTAGCTAATCAGGGGTT
TTTTATTCATATAGTGGAGAAAAGCCCCTGTATCACACCCGCGGCTAACGCACCCTTTGATTTGCGCGTGTCAGCTTTTA
GTGCTCAGAGTAAACGTTTGCTTGAGCTTGCTGGCGTGTGGCAGAACCTTCCGCCAAACCGTTTGTGTGCTTATCAGGGA
TTACAAACCTGGGAAAAGGGCAGTCAAAAACTGAAATTTGACAGTCAGGAGATTGGTATAAATGCATTAGGCTACATTGC
GGAAAATAACTGGGTGCAAGCCGTCTTATGGCAGGCATTAAAAAAACTTTCGAACGTCACCTGTTATGAAAATAGAGAAG
TTAAGCGCATTATTAATTCAGATAAAAGCGTATTGATTAGTTTAGATAATGAGGAGCAGATTAAAGCGGAATTATTAATC
GCCTGTGACGGGGCTAATTCAGCTGTTCGCCAACTGCTGGATATTGGTATTACTGCTTGGGATTATCGTCAGGAATGTAT
GTTGGTAACTATTAAAACCGATTGCCCGCAGCAAAATATTACCTGGCAGGAATTTCGTGAAACCGGTCCCTGTGCCTTTT
TGCCGCTGGCAGAAAACAATGCCAGCTTAGTTTGGTACCATAATCCACAAAAAATCGGCCATTTAAAATCGCTCTCCAAT
GCGCAGTTAAAAGAGGTCATTAAAGATGAATTCCCGCCTCTGGATTTTGACTTTGAGATCGTAGATAAAGGGTCTTTCCC
GCTTACCCGTCGGCATGCACAATCTTATTTTAAAGGATCCTGCGTTTTACTGGGAGATGCCGCACATACTATTAATCCAC
TGGCGGGCCAGGGGGTAAACCTGGGGTTTAAAGATGTTGAGTGTTTAGTTGATTTATTGCTGAATTGCTCAGATTTACCG
ATGCAAACTTTGTTAAAACGTTACCAGTGGCAGCGTAAACCGGCTAATTTATTGATGCAGAGCGCAATGGACGTATTTTA
TAAAGTTAGTAAGAGTGACCTTGGCCCGGTGCGTTTTATGCGCCGCGGCGTGCTAGGATTGGCGCAGAAAAGTGGGCCGC
TTAAGAATAAAGTCATGAAGTATGCAATGGGGTTGTAA

Upstream 100 bases:

>100_bases
TCCTTTTTTAAGATTAATAATGGTTTGTATTTTGTGATTTTAAGGATAATTTAAGTACAATAAAAATCGTGCTATAAAGA
AAATAACGGAGGGAATAAAA

Downstream 100 bases:

>100_bases
GCGCAATGGAGGTATTTCATAACGCAAGCAATAGTGGCCTTGGCTCGTCGTTGCGTTTAATACGGCTAGGAATGTTAGGA
TTGGCGCAGAGAGGCGGGCC

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase UbiF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MQRREIIVVGGGMVGALSALLLANQGFFIHIVEKSPCITPAANAPFDLRVSAFSAQSKRLLELAGVWQNLPPNRLCAYQG
LQTWEKGSQKLKFDSQEIGINALGYIAENNWVQAVLWQALKKLSNVTCYENREVKRIINSDKSVLISLDNEEQIKAELLI
ACDGANSAVRQLLDIGITAWDYRQECMLVTIKTDCPQQNITWQEFRETGPCAFLPLAENNASLVWYHNPQKIGHLKSLSN
AQLKEVIKDEFPPLDFDFEIVDKGSFPLTRRHAQSYFKGSCVLLGDAAHTINPLAGQGVNLGFKDVECLVDLLLNCSDLP
MQTLLKRYQWQRKPANLLMQSAMDVFYKVSKSDLGPVRFMRRGVLGLAQKSGPLKNKVMKYAMGL

Sequences:

>Translated_385_residues
MQRREIIVVGGGMVGALSALLLANQGFFIHIVEKSPCITPAANAPFDLRVSAFSAQSKRLLELAGVWQNLPPNRLCAYQG
LQTWEKGSQKLKFDSQEIGINALGYIAENNWVQAVLWQALKKLSNVTCYENREVKRIINSDKSVLISLDNEEQIKAELLI
ACDGANSAVRQLLDIGITAWDYRQECMLVTIKTDCPQQNITWQEFRETGPCAFLPLAENNASLVWYHNPQKIGHLKSLSN
AQLKEVIKDEFPPLDFDFEIVDKGSFPLTRRHAQSYFKGSCVLLGDAAHTINPLAGQGVNLGFKDVECLVDLLLNCSDLP
MQTLLKRYQWQRKPANLLMQSAMDVFYKVSKSDLGPVRFMRRGVLGLAQKSGPLKNKVMKYAMGL
>Mature_385_residues
MQRREIIVVGGGMVGALSALLLANQGFFIHIVEKSPCITPAANAPFDLRVSAFSAQSKRLLELAGVWQNLPPNRLCAYQG
LQTWEKGSQKLKFDSQEIGINALGYIAENNWVQAVLWQALKKLSNVTCYENREVKRIINSDKSVLISLDNEEQIKAELLI
ACDGANSAVRQLLDIGITAWDYRQECMLVTIKTDCPQQNITWQEFRETGPCAFLPLAENNASLVWYHNPQKIGHLKSLSN
AQLKEVIKDEFPPLDFDFEIVDKGSFPLTRRHAQSYFKGSCVLLGDAAHTINPLAGQGVNLGFKDVECLVDLLLNCSDLP
MQTLLKRYQWQRKPANLLMQSAMDVFYKVSKSDLGPVRFMRRGVLGLAQKSGPLKNKVMKYAMGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=414, Percent_Identity=26.8115942028986, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI32967307, Length=348, Percent_Identity=27.0114942528736, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786883, Length=388, Percent_Identity=43.0412371134021, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI1789273, Length=397, Percent_Identity=33.5012594458438, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789274, Length=339, Percent_Identity=27.7286135693215, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25146631, Length=439, Percent_Identity=28.0182232346241, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321694, Length=450, Percent_Identity=26.4444444444444, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19920760, Length=384, Percent_Identity=26.8229166666667, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43104; Mature: 43104

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRREIIVVGGGMVGALSALLLANQGFFIHIVEKSPCITPAANAPFDLRVSAFSAQSKRL
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHH
LELAGVWQNLPPNRLCAYQGLQTWEKGSQKLKFDSQEIGINALGYIAENNWVQAVLWQAL
HHHHHHHHCCCCCHHHHHHCHHHHHCCCCEECCCHHHCCCEEEEEEECCCHHHHHHHHHH
KKLSNVTCYENREVKRIINSDKSVLISLDNEEQIKAELLIACDGANSAVRQLLDIGITAW
HHHCCCCCCCCCHHHHHHCCCCEEEEEECCCHHEEEEEEEEECCCCHHHHHHHHCCCCEE
DYRQECMLVTIKTDCPQQNITWQEFRETGPCAFLPLAENNASLVWYHNPQKIGHLKSLSN
CCCCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCEEEEEECCHHHHHHHHCCH
AQLKEVIKDEFPPLDFDFEIVDKGSFPLTRRHAQSYFKGSCVLLGDAAHTINPLAGQGVN
HHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCC
LGFKDVECLVDLLLNCSDLPMQTLLKRYQWQRKPANLLMQSAMDVFYKVSKSDLGPVRFM
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
RRGVLGLAQKSGPLKNKVMKYAMGL
HHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQRREIIVVGGGMVGALSALLLANQGFFIHIVEKSPCITPAANAPFDLRVSAFSAQSKRL
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHH
LELAGVWQNLPPNRLCAYQGLQTWEKGSQKLKFDSQEIGINALGYIAENNWVQAVLWQAL
HHHHHHHHCCCCCHHHHHHCHHHHHCCCCEECCCHHHCCCEEEEEEECCCHHHHHHHHHH
KKLSNVTCYENREVKRIINSDKSVLISLDNEEQIKAELLIACDGANSAVRQLLDIGITAW
HHHCCCCCCCCCHHHHHHCCCCEEEEEECCCHHEEEEEEEEECCCCHHHHHHHHCCCCEE
DYRQECMLVTIKTDCPQQNITWQEFRETGPCAFLPLAENNASLVWYHNPQKIGHLKSLSN
CCCCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCEEEEEECCHHHHHHHHCCH
AQLKEVIKDEFPPLDFDFEIVDKGSFPLTRRHAQSYFKGSCVLLGDAAHTINPLAGQGVN
HHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCC
LGFKDVECLVDLLLNCSDLPMQTLLKRYQWQRKPANLLMQSAMDVFYKVSKSDLGPVRFM
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
RRGVLGLAQKSGPLKNKVMKYAMGL
HHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]