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The map label for this gene is rumA

Identifier: 119944302

GI number: 119944302

Start: 675332

End: 676666

Strand: Direct

Name: rumA

Synonym: Ping_0529

Alternate gene names: 119944302

Gene position: 675332-676666 (Clockwise)

Preceding gene: 119944300

Following gene: 119944303

Centisome position: 14.81

GC content: 40.82

Gene sequence:

>1335_bases
ATGGCCCAGTTCTTTAAAGCAGCACCCACAAATAGTATCAAAAATCATATTTTAAAAAACATAAAAGTTGAAAAATTAGA
TCACCGGGGACGTGGTTTAGCCTATTTTCAGAATAAACCTCTTTTTATTGATGGGGCGCTTGCAGGTGAATTACTTGAAG
TACAAATTGTAGAAAGTAAAAAACGCTATAGCAAAGGGAAGATTAAAAAGATCATCAAGGCCAGTGAGTTAAGAATTACT
GCCGCTTGTCCTCATTATCAGGAGTGCGGTGGCTGTGATCTGCAGCATCTAAACCAAGCTGCGCAGATCCAGATTAAATC
AGATGGGCTATTATCATTATTTCAACGTTTTGCTAAAAAAGTACCGCAACAACTGGAAAAACCGATTATTGATAAGGCAT
GGGAATATCGTCGTACAGCGCGTTTTGGTTTACAATTTGATAAAAAAAATAAACAGTTAAAAATGGGCCTTAGACGCGCC
CAAAGTAATGAGCTTATCGACCAAAAAGTTTGCCCTGTATTATTACCTGAATTAGAGTGTTTAATTCCGCCCCTTAAAAT
ATTATTAAATAGCTTACAATGCAAAGCCCATTTAGGGCATGTGGAGCTGCTTTACGCAGATCAAGGTGCGGTGGTTTTAT
TACGTCATATGAAAACCTTAACCAGCCCGGATTTACAACTAATAACGGCTTTTTCAGCGCTACAAAAAGTGAACTTTTTT
GGACAGGCTTCCTCTAACCAGTCTGTTTGTCTGGCCGGCGAGGCAAATCTAAGTTACCGGTTGCCGGAGTGGGATTGTTC
CTTGAGTTTTACACCCACCGATTTTTTACAAGTAAACAGTGATATTAATAAAAAAATGGTCAGTCAGGCTATGCAGTGGC
TCGCTTTAGAAAAAAACGACAGTGTCTTAGATCTTTTCTGCGGTTTAGGGAATTTCACTTTACCGATCGCGCGCCAGGTT
GAATCGGTAGTGGGTATTGAAGGGGTTCAACAAATGGTGGACCGTGCAACGGCCAATGCACAATTAAATAATTTACAGAA
TGCCCGATTTTATCAAGCCGACTTAAGCGGCGAAAATCTTATCGAGCAGGAGTGGGCCAACCAAAACTTTAATAAGGTTT
TATTAGATCCGGCGCGTGCTGGCGCGTTGGATTGTTTAGCTTTTATAGCACAAAAAAAACCATCGCATATCCTCTATGTA
TCCTGTGATCCGCTGACCCTGGCCCGGGATAGTCAAGTGTTGCTTGATAAGGGTTATAAACTGGATAAATTAGGTTTGTT
GGACATGTTCCCGCAAACGGCTCATATGGAATCGATGGCACTGTTTACTGGTTAG

Upstream 100 bases:

>100_bases
TTTATATAAATGAATGCTTTTATTTTTCATTGTGTGACAATAGTCTTATCGATAAATATCAGTCTATGATCGCATAGCTT
ACTTAACCTGTGAATCACTT

Downstream 100 bases:

>100_bases
ATTTATTTTTATTTTTTGGAGTTTCTTTTAATGGTTTCAGTCAGAGATACACATTTTTATCAAACCGCGCAAAAAAACCC
TAAAAAATGGGTCACTACGT

Product: RNA methyltransferase, TrmA family protein

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAQFFKAAPTNSIKNHILKNIKVEKLDHRGRGLAYFQNKPLFIDGALAGELLEVQIVESKKRYSKGKIKKIIKASELRIT
AACPHYQECGGCDLQHLNQAAQIQIKSDGLLSLFQRFAKKVPQQLEKPIIDKAWEYRRTARFGLQFDKKNKQLKMGLRRA
QSNELIDQKVCPVLLPELECLIPPLKILLNSLQCKAHLGHVELLYADQGAVVLLRHMKTLTSPDLQLITAFSALQKVNFF
GQASSNQSVCLAGEANLSYRLPEWDCSLSFTPTDFLQVNSDINKKMVSQAMQWLALEKNDSVLDLFCGLGNFTLPIARQV
ESVVGIEGVQQMVDRATANAQLNNLQNARFYQADLSGENLIEQEWANQNFNKVLLDPARAGALDCLAFIAQKKPSHILYV
SCDPLTLARDSQVLLDKGYKLDKLGLLDMFPQTAHMESMALFTG

Sequences:

>Translated_444_residues
MAQFFKAAPTNSIKNHILKNIKVEKLDHRGRGLAYFQNKPLFIDGALAGELLEVQIVESKKRYSKGKIKKIIKASELRIT
AACPHYQECGGCDLQHLNQAAQIQIKSDGLLSLFQRFAKKVPQQLEKPIIDKAWEYRRTARFGLQFDKKNKQLKMGLRRA
QSNELIDQKVCPVLLPELECLIPPLKILLNSLQCKAHLGHVELLYADQGAVVLLRHMKTLTSPDLQLITAFSALQKVNFF
GQASSNQSVCLAGEANLSYRLPEWDCSLSFTPTDFLQVNSDINKKMVSQAMQWLALEKNDSVLDLFCGLGNFTLPIARQV
ESVVGIEGVQQMVDRATANAQLNNLQNARFYQADLSGENLIEQEWANQNFNKVLLDPARAGALDCLAFIAQKKPSHILYV
SCDPLTLARDSQVLLDKGYKLDKLGLLDMFPQTAHMESMALFTG
>Mature_443_residues
AQFFKAAPTNSIKNHILKNIKVEKLDHRGRGLAYFQNKPLFIDGALAGELLEVQIVESKKRYSKGKIKKIIKASELRITA
ACPHYQECGGCDLQHLNQAAQIQIKSDGLLSLFQRFAKKVPQQLEKPIIDKAWEYRRTARFGLQFDKKNKQLKMGLRRAQ
SNELIDQKVCPVLLPELECLIPPLKILLNSLQCKAHLGHVELLYADQGAVVLLRHMKTLTSPDLQLITAFSALQKVNFFG
QASSNQSVCLAGEANLSYRLPEWDCSLSFTPTDFLQVNSDINKKMVSQAMQWLALEKNDSVLDLFCGLGNFTLPIARQVE
SVVGIEGVQQMVDRATANAQLNNLQNARFYQADLSGENLIEQEWANQNFNKVLLDPARAGALDCLAFIAQKKPSHILYVS
CDPLTLARDSQVLLDKGYKLDKLGLLDMFPQTAHMESMALFTG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=189, Percent_Identity=32.2751322751323, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI51173878, Length=189, Percent_Identity=32.2751322751323, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=43.2735426008969, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=30.0546448087432, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787083, Length=180, Percent_Identity=28.3333333333333, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24666579, Length=189, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_PSYIN (A1SSB8)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941982.1
- ProteinModelPortal:   A1SSB8
- SMR:   A1SSB8
- STRING:   A1SSB8
- GeneID:   4624841
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0529
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   FFRRRSH
- PhylomeDB:   A1SSB8
- BioCyc:   PING357804:PING_0529-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 49785; Mature: 49654

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 402-402 BINDING 277-277 BINDING 306-306 BINDING 311-311 BINDING 327-327 BINDING 354-354 BINDING 376-376

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAAPTNSIKNHILKNIKVEKLDHRGRGLAYFQNKPLFIDGALAGELLEVQIVESK
CCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEECCCCEEEECCCCCCEEEEEEEHHH
KRYSKGKIKKIIKASELRITAACPHYQECGGCDLQHLNQAAQIQIKSDGLLSLFQRFAKK
HHHHHHHHHHHHHHHHEEEEECCCCHHHCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHH
VPQQLEKPIIDKAWEYRRTARFGLQFDKKNKQLKMGLRRAQSNELIDQKVCPVLLPELEC
HHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LIPPLKILLNSLQCKAHLGHVELLYADQGAVVLLRHMKTLTSPDLQLITAFSALQKVNFF
HCCHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GQASSNQSVCLAGEANLSYRLPEWDCSLSFTPTDFLQVNSDINKKMVSQAMQWLALEKND
CCCCCCCCEEEEECCCCEEECCCCCCCEECCCHHHEEECCCHHHHHHHHHHHHHHCCCCC
SVLDLFCGLGNFTLPIARQVESVVGIEGVQQMVDRATANAQLNNLQNARFYQADLSGENL
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCHH
IEQEWANQNFNKVLLDPARAGALDCLAFIAQKKPSHILYVSCDPLTLARDSQVLLDKGYK
HHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEECCCCHHHHHCCCC
LDKLGLLDMFPQTAHMESMALFTG
CCCCCHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AQFFKAAPTNSIKNHILKNIKVEKLDHRGRGLAYFQNKPLFIDGALAGELLEVQIVESK
CCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEECCCCEEEECCCCCCEEEEEEEHHH
KRYSKGKIKKIIKASELRITAACPHYQECGGCDLQHLNQAAQIQIKSDGLLSLFQRFAKK
HHHHHHHHHHHHHHHHEEEEECCCCHHHCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHH
VPQQLEKPIIDKAWEYRRTARFGLQFDKKNKQLKMGLRRAQSNELIDQKVCPVLLPELEC
HHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LIPPLKILLNSLQCKAHLGHVELLYADQGAVVLLRHMKTLTSPDLQLITAFSALQKVNFF
HCCHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GQASSNQSVCLAGEANLSYRLPEWDCSLSFTPTDFLQVNSDINKKMVSQAMQWLALEKND
CCCCCCCCEEEEECCCCEEECCCCCCCEECCCHHHEEECCCHHHHHHHHHHHHHHCCCCC
SVLDLFCGLGNFTLPIARQVESVVGIEGVQQMVDRATANAQLNNLQNARFYQADLSGENL
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCHH
IEQEWANQNFNKVLLDPARAGALDCLAFIAQKKPSHILYVSCDPLTLARDSQVLLDKGYK
HHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEECCCCHHHHHCCCC
LDKLGLLDMFPQTAHMESMALFTG
CCCCCHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA